sticky ends 中文意思是什麼
sticky ends
解釋
粘狀末端-
The pcr products were purified and linked with pmd 18 - t vector respectively. the positive recombinants were selected and digested with double restriction enzymes respectively. goat fsh - a and p subunit genes which had sticky ends were purified and linked respectively with pf which also had the corresbonding sticky ends and were purified
將所得的山羊fsh - 、亞基基因分別用加相應限制性內切酶酶切位點的引物大量擴增，回收后與pmd18 - t連接，篩選出陽性重組子，大量雙酶切回收獲得帶粘性末端的山羊fsh - 、亞基基因，將它們分別與同兩個酶大量雙酶切回收獲得的帶粘性末端的pf質粒進行連接，篩選陽性重組子。
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