streptomyces avermitilis 中文意思是什麼

streptomyces avermitilis 解釋
及營養條件對阿佛曼菌
  • streptomyces : n. （pl. streptomyces ） 【生物學】鏈絲菌屬 （Streptomyces） 菌。
  1. In addition to avermectins, s. avermitilis produces oligomycin, a strongly toxic compound. gene deletion vector pxl05 was used to disrupt oligomycin polyketide synthase ( pks ) encoding genes ( olma ) in streptomyces avermitilis cz8 - 73, the producer of anthelmintic avermectins b and the cell growth inhibitor oligomycin. olma gene cluster in the chromosome was displaced by deletion allele on the plasmid via double crossover

    本研究以產阿維菌素b和寡黴素的阿維鏈黴菌cz8 - 73為出發菌株，構建了基因缺失載體pxl05 ，並將其轉入cz8 - 73中，通過缺失載體和染色體之間的同源雙交換，對染色體上長達90kb的寡黴素聚酮合酶（ pks ）基因簇（ olma ）進行了缺失。
  2. Avermectins are a series of 16 - membered macrocyclic lactones produced by streptomyces avermitilis with potent anthelmintic and insecticidal activity. they are the most effective agricultural pesticides and antiparasitic agents, and used widely in medical, veterinary and agricultural fields

    阿維鏈黴菌（ streptomycesavermitilis ）產生的阿維菌素（ avermectins ）是一組高效、低毒、廣譜的殺蟲抗生素，在醫藥、農業和畜牧業生產中具有良好的應用價值和廣闊的市場前景。
  3. Content : avermectins are a class of drugs which have been found wide usage against a board spectrum of nematodes and arthropods, many of which are important endoparasites and ectoparasites of animals. avermectin bi is a common agriculture pesticide produced by the actinomycete streptomyces avermitilis and registered for worldwide use

    正文：阿維菌素類藥物（ avermectins ， avms ）屬大環內酯類抗生素，由於其優異的驅蟲活性和較高的安全性，被視為目前最為優良、應用最廣泛的獸用驅蟲藥。
  4. Effect of avec gene deletion on avermectins production in streptomyces avermitilis

    基因缺失對產素調控的影響
  5. The transformants were cultivated and the harvested cells were used in the procedure of conjugation by which the recombinant plasmid pid03 and pc05 were introduced into streptomyces avermitilis s - 2 respectively

    經過在mym平板上的傳代和抗性標記的篩選分別得到了同源雙交換的菌株aved24和avecg 。
  6. In this study, the avermectin - producing strain streptomyces avermitilis was studied and the avermectin biosynthesis gene cluster in the genomic dna of streptomyces avermitilis s - 2 was altered by the method of gene engineering. insertion inactivation of aved gene in the cluster by introducing apramycin resistance gene into aved gene resulted in the disappearance of " a " components of avermectins. when avec gene was inactivated by the same way, four " 1 " components were lost and only " 2 " components, the potential precursor of ivermectin, were accumulated

    將該基因簇中的aved基因通過插入外源的安普黴素抗性基因片段使其失活，導致發酵產物中4個a組分（不需要的組分）的消失；將基因簇中的avec基因通過同樣手段，使其失活，導致發酵產物中4個「 1 」組分的消失，而主要積累「 2 」組分（進一步改造可成為伊維菌素的前體b _ 2組分） 。
  7. Four types of colony, powdery gray, gray with white blot, white and bald were isolated from streptomyces avermitilis among them only the powdery gray one produced the highest avermectin. the gray with white blot one only produced 70 % - 80 % less than the powdery gray. and the others produced none

    白色菌株和光禿型菌株經傳代培養比較穩定，灰色有白點的菌落在傳代過程中不斷分化出灰色、白色、光禿型菌落，直接造成遺傳的不穩定性，使生產效價不穩定，為生產的大害。
  8. In order to obtain a dna fragment containing aved gene, 5 ' end primer and 3 ' end primer, consisting of 18 and 20 nucleotides respectively, were designed and synthesized. a 2. 2 kb dna fragment containing aved gene was obtained by pcr when genomic dna of streptomyces avermitilis s - 2 was used as a template

    為了得到aved基因片段，首先根據已知的阿維菌素生物合成基因簇的核苷酸序列，設計併合成了由18和20個核苷酸組成的5端和3端引物；以streptomycesavermitiliss - 2基因組dna為模板，經pcr擴增得到了2 . 2kb的含有aved基因的dna片段。
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