subcellular localization 中文意思是什麼
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In our experiment, after light and dark adaptation, the retina of the macrobrachium rosenbergi was respective incubated in high calcium solution, physiological solution and low calcium solution. we studied the effect of calcium concentration on the content and subcellular localization of gq protein a subunit in the photoreceptor cell of macrobrachium rosenbergi on light adaptation and dark adaptation by sds - page technology and imunoelectron microscopy technology. our study results indicated : 一 、 effects of calcium concentration on the soluble gq protein a subunit in the photoreceptor cell of macrobrachium rosenbergi on light adaptation and dark adaptation
而鈣離子對gq蛋白亞基活性有無影響還未見報道。我們以光適應和暗適應條件下的羅氏沼蝦復眼視網膜為材料，分別用高鈣溶液、生理溶液、低鈣溶液孵育后，通過sds ? page電泳技術及免疫膠體金電鏡技術，研究鈣離子濃度對光暗適應時羅氏沼蝦感光細胞gq蛋白亞基含量的影響及亞基亞細胞定位的影響。 -
Prediction of subcellular localization of proteins for gram - negative bacteria
革蘭氏陰性菌中蛋白質亞細胞定位預測 -
An unusual rice calmodulin isoform, oscam61, was first obtained in our lab, which contains an n - terminal cam domain and a c - terminal basic extension with a potential prenylation site. in vitro activity assays confirm oscam61 as a functional calmodulin. using the green fluorescent protein ( gfp ) as a visual marker, we further studied subcellular localization of oscam61 in stably transformed tobacco cells
利用綠色熒光蛋白（ greenfluorescentprotein ， gfp ）作為標記，研究了oscam61在煙草細胞中的定位， gfp - oscam61融合蛋白（具有開放的異戊烯化修飾位點）定位於細胞質膜和細胞器膜上，而oscam61 - gfp （異戊烯化修飾位點被gfp封閉）定位於細胞核的核質中。 -
Subcellular localization by immunofluorescence revealed that two rat isoforms are associated with the autophagic pathway. these results are help to discover the mechanisms and functions of autophagy in vivo
另外，通過熒光定位以及與mdc的共定位分析發現兩個基因與大鼠的lc3相似，也是定位在自噬體上。 -
Then using ecbp21 antibody and immunogold transmission electron microscopy method, we studied the subcellular localization of ecbp21. the results indicated that the gold particles were mainly localized in the cell wall in callus cells and rachis cells of angelica dahurica. these results indicated that ecbp21 mainly localized in cell wall, which provide a direct evidence of the extracellular existence of ecbp21. furthermore, using ecbp21 antibody and immunohistochemical method, we studied the organic specially distribution of ecbp21, the results indicated that ecbp21 distributed in all organize, but it distributed more in leave n flower rachis than in leafstalk and root
首先，構建了ecbp21表達載體，誘導了重組蛋白的表達，並通過膠回收法獲得了大量純化重組ecbp21蛋白，制備了高效價、高特異性抗體；隨后，利用ecbp21抗體，結合免疫膠體金電鏡定位技術進行了ecbp21亞細胞定位研究，結果顯示：在白芷愈傷組織細胞和花序軸細胞中金顆粒主要分佈在細胞壁區域，而在細胞內未發現或僅有少量金顆粒分佈，表明ecbp21蛋白主要定位於細胞壁區域，這為細胞外cambp （ ecbp21 ）的胞外存在提供了直接證據：進一步，利用ecbp21抗體，通過免疫組織化學分析研究了ecbp21組織特異性分佈狀況，結果表明ecbp21在白芷各組織中均有分佈，但在葉、花、花序軸中分佈較多，而在葉柄、根中分佈較少。 -
Studies on the subcellular localization of gfp - trpt1 / pit13 showed the trpt1 and pit 13 proteins localized predominantly to nuclear bodies. this is consistent with where the trpt1 functions
Trpti與gfp融合蛋白在細胞內的表達結果，顯示trpti主要分佈在細胞核內，這與其具有的參與trna剪接成熟的功能是相符合的。 -
三 、 effects of calcium concentration on subcellular localization of gq protein a subunit in the photoreceptor cell of macrobrachium rosenbergi on light adaptation and dark adaptation. effects of calcium concentration on subcellular localization of gq protein a subunit in the photoreceptor cell of macrobrachium rosenbergi on light adaptation and dark adaptation were studied using immunogold electronmicroscopy
三、鈣離子濃度對光暗適應羅氏沼蝦感光細胞gq蛋白q亞基亞細胞定位的影響採用免疫膠體金電鏡技術，研究光暗適應條件下不同鈣離子濃度對羅氏沼蝦感光細胞中gq蛋白。 -
The cellular localization of hse was done by using in situ hybridization, the results showed that primary spermtatocytes and spermatids in seminiferous tubular have positive signals. the subcellular localizations of hsei and hseii were identified by gfp fusion protein. hsei - gfp fusion protein was evenly distributing in cytoplasm, while hseii - gfp was not evenly distributing in cytoplasm and there were many bright spots in cytosol
用gfp高合蛋白的技術確定hsei和hsell在細胞中勺定位，結果顯示hsei gfp均勻地分佈於細胞漿中，而hsell0fp則分佈不均勻，在胞漿有聚集成許多熒光信號很強的點。 -
In order to understand the apoplast cam gene and the relationship between the apoplast cam and cam isoforms, we intended to get transgenic plants harboring soybean calmodulin isoform genes ( scams ) fused to gfp as a reporter and study the subcellular localization of scams
為了進一步利用分子生物學方法在基因水平上研究胞外cam的存在，同時研究胞外cam與cam亞型之間的關系，本論文利用綠色熒光蛋白（ gfp ）作為報告基因研究了大豆cam基因家族（ scams ）的亞細胞定位。 -
Subcellular localization by cell fractionation, immunofluorescence revealed that three human isoforms were associated with membranes involved in the autophagic pathway. the glycine and lysine respectively decided the location of autophagosome of human map1lc3a and map1lc3b. in addition, our results indicated that other proteins were involved in the post tranlational modification of human map1lc3b and this modication could be induced by amino acid starvation
人lc3a的膜定位與gly120位點的切割和修飾緊密相關，而人lc3b的膜定位是與lys122相關，進一步證明了修飾方式的特異性，對人lc3b的進一步研究表明參與其修飾的分子也與其它基因不同，並且饑餓可以誘導其修飾的發生。 -
For further identification the existence of the extracellular ecbp21, we used immunogold localization technology to study the subcellular localization of ecbp21
為了進一步確定ecbp21的胞外存在，本實驗利用免疫金標定位技術，研究了ecbp21亞細胞定位狀況。 -
To study the biological function of sh2a gene, we constructed its recombinant expression vec - tor, and investigated its function by cell transfection, kinase assay, subcellular localization and expression analysis. materials and methods 1
我們構建了sh _ 2a基因的真核重組表達載體，通過細胞轉染、激酶活性檢測、亞細胞定位、表達分析和流式細胞儀等方法對其功能進行了初步的研究。
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