t-dna 中文意思是什麼

t-dna 解釋
acid]轉運脫氧核糖核酸
  • t : 中世紀羅馬數字的160。
  • dna : 1. deoxyribonucleic acid 【生物化學】去[脫]氧核糖核酸。2. deoxypentose-nucleic acid 去[脫]氧戊糖核酸。
  1. Unlilie other cells that also use the salvage pathway for purine biosynhesis, prolfferating b and t cells are solely dependent on the de novo pathway for the generation of wsine. by blocking impdh, b and t cells cannot synthesise the necessary levels of ana and dna to molm a proliferathe response to ags and ndogens

    根據對balb c和c57bl 6小鼠互為供、受者進行移植的結果表明，在排斥發生時間上不同的鼠株移植組合存在差異b 0刀勻， balb兒小鼠的同種應答強度較c57bl 6小鼠為弱。
  2. Degenerate oligonucleotides to highly conserved regions of cucumis melo 1 - aminocyclopropane - 1 - carboxylic acid ( acc ) oxidase gene were used to prime the amplification of fragment of 128bp by ploymerase chain reaction ( pcr ) in samples of genomic dna from fruit of cucumis melo l. cv hetao flesh, which was cloned into plasmid vector pmd - 18 - t. the clon of antisense orientation were selected, and it was inserted downstream of camv35s promoter and enhancer " " of tmv into the plant expression vector pbinyxw, antisence expression vector pbinya was constructed. at the base that pollination and fertilization of cucumis melo l. cv hetao was studied, using pollen tube pathway transformate cucumis melo l. cv hetao, 76 fruit had been obtained, moreover, hardness and content of sugar were analysed

    本實驗以河套蜜瓜果肉基因組dna為模板，用甜瓜acc氧化酶基因特異寡核苷酸鏈為引物進行pcr擴增，得到128bp的擴增產物。將得到的擴增產物克隆到質粒載體pmd - 18 - t上，篩選反向克隆，然後將其反向構建到植物表達載體pbinyxw的camv35s啟動子和tmv增強子「 」的下游，構建成反義表達載體pbinya 。並在對河套蜜瓜授粉受精生物學研究的基礎上，通過花粉管通道法轉化河套蜜瓜，共獲76顆瓜，並進行了硬度和含糖量的分析。
  3. Two captive populations could n ' t be defined as separate evolutionary significant units ( esus ) because of lacking of genetic divergence between them, and should be considered as a single esu in the conservation of the species. by comparing the sequences of control region of mitochondrial dna from three species of crocodiles, it is revealed that the smallest genetic diversity exists between alligator sinensis and alligator mississipiensis. a portion of mitochondrial nd4 and cytochrome b gene of 3 species of crocodilian was sequenced

    百年來關于揚子鱷的分類地位存在著很多爭議，本文利用測得的揚子鱷（ alligatorsinensis ） 、暹羅鱷（ crocodlylussiamensis ）和灣鱷（ crocodylusporosus ）的mtdnand4和cytb基因序列，以及從genbank中獲得密西西比鱷、凱門鱷和海龜（ cheloniamydas ）的nd4基因和cytb基因相應片段，構建以海龜為外群的系統進化樹。
  4. Normal dna consists of four bases - adenine, cytosine, guanine and thymine ( known as a, c, g, t ) - molecules that spell out the genetic code in pairs

    正常dna由四種堿基組成腺嘌呤、胞嘧啶、鳥嘌呤、胸腺嘧啶，這些分子以成對的方式組成基因的密碼。
  5. In this paper, first strand cdna of 3abc gene was synthesized using template rna extracted from cells infected with fmdv. the complete 3abc gene about isoobp was amplified by pcr and ligated into pgem - t easy vector. after transforming e. coli dh5 a, ampicillin resistant colonies were isolated and plasmid dna was prepared and analyzed by restriction analysis and pcr. presence of the full length 3abc gene was verified by nucleotide sequence analysis and the plasmid containing the expected sequence was named as pgem - 3abc. comparing the aquired sequence of 3abc with that of reference strains, the homology is more than 99 percent. the pgem - 3abc was digested with sal i and bgl ii and ligated into xho i and bgl ii - digested expression vector ptriex - 4 neo. lt was identified by restriction analysis and pcr and sequencing that this fragment had a 17bp deletion hi the nucleotide sequence 708bp of 3abc gene, which happened to form a terminator codon behind 3ab gene, but it contained the complete open reading frame ( orf ) of 3ab gene. positive clones were selected and induced with lmmol / l isopropyl - d - galactoside ( iptg ), bacteria were detected by sds - page and western blotting after properly treated. the results showed that the 3ab gene expressed successfully in e. coli and 33. 5ku fusion protein can be recognized by the positive bovine serum of fmdv. the amount of target protein is over 26 % of the total bacteria protein by gel thin layer scanning analysis

    擴增產物連接到pgem - teasy載體中，轉化大腸桿菌dh5菌株，篩選氨芐青霉素抗性菌落，提取質粒經酶切鑒定、 pcr分析以及確證性測序證明，所克隆的1500bp左右的片段含有完整的3abc基因，與國外參考序列相比，同源性在99以上。將重組質粒pgem - 3abc和表達載體ptriex - 4neo分別用sal和bgl與xho和bgl消化后，亞克隆3abc基因至原核表達載體ptriex - 4neo中，通過酶切鑒定、 pcr擴增以及序列分析，發現克隆到ptriex - 4neo載體上的片段於3abc基因708bp處出現了17bp的缺失，碰巧在3ab基因后形成一終止密碼子，但3ab基因的閱讀框架完整，選出含有3ab基因完整閱讀框架的陽性克隆，用iptg誘導表達，收集菌液進行sds - page電泳、 westernblotting分析，結果表明， 3ab基因在大腸桿菌中成功表達，其表達產物為分子量33 . 5ku的融合蛋白，並能被口蹄疫病毒陽性血清識別。經薄層掃描分析，表達量占總蛋白量的26以上。
  6. As analyzed, ( 1 ) the rapd technique is highly sensitive to investigating genetic diversity in t. lepturus and e. muticus. t. lepturus exhibits lower polymorphism and genetic diversity than e. muticus ; ( 2 ) according to the analysis of the partial mitochondrial 16s rrna gene sequences, a very low intraspecific variation and considerably high divergence among species were found, which reveals a dual nature of conservatism and variability in mitochondrial 16s rrna gene ; ( 3 ) five primers generate the species - speeific rapd sites and these sites can be served as the molecular markers for species identification and ( 4 ) it can be proved at dna variation level that t. lepturus and e. muticus are of two species respectively pertainiag to different genera, which supported the nelson taxonomic conclusion

    分析結果表明: （ 1 ） rapd技術研究黃海帶魚和小帶魚的遺傳多樣性具有較高的靈敏度和檢出率，帶魚的多態比例和遺傳多態度均較小帶魚的低; （ 2 ）線粒體165出兇a基因序列在分析兩物種遺傳變異時表現出保守和變異的雙重特性，種內變異極小而種間較大: （ 3 ） 5個隨機引物擴增出種特異的ra衛d帶，可作為種間分子鑒定標記; （ 4 ）研究證實帶魚和小帶魚是不同屬的兩個種，從而在分子水平上支持了nelson分類系統的觀點。
  7. At the same time, using the genomic dna of blast sensitive cultivar c039 as a template, two fragments were obtained that were the same large dna sequence as resistance cultivar c101a51. one sequence has conserved motifs of nbs - lrr type resistance genes, the other sequence can " t read through

    同時本研究還以感病品種c039的基因組為模板，也擴增得到與c101a51抗病品種中抗病基因同源序列同樣大小的dna片段，但一個有抗病基因的保守結構，另一個片段不能通讀。
  8. Secondly, the trends of dna and rna in the oocytes and follicle cells were displayed by histochemical method during oogenesis, which indicated that rna were synthesized at stage of the vitellogenic oocytes. but the quantity of dna was n ' t change

    並根據這八個階段對卵子發生進行了組織化學的初步研究，結果表明，在卵子發生過程中，卵母細胞遺傳物質dna的總量相對保持不變，保證了遺傳信息的。
  9. Four out of 150 random primers could detect dna polymorphism between the two mutants and the wild type arabidopsis thaliana, which provided a strong evidence for the truth of the mutants. only one primer gave a specific dna fragment about 1200bp which two mutants own but the parent do n ' t. we drew a preliminary conclusion that the fragment might be related to the salt - tolerance gene

    擬南芥抗鹽突變體的rapd分析結果表明， 150條引物中有四條引物在突變體和野生型擬南芥之間擴增出多態性，證明了dna水平突變的發生，其中1條引物在突變體的擴增產物比在野生型的擴增產物多出一個大小約為1200bp的片段，初步認為該片段可能與植物的抗鹽性有關。
  10. In this study, dna strand breaks in jm109 cells caused by - rays and the protective effect of lexa protein of deinococcus radiodurans have been studied by fluorometric assay of dna unwinding., the results indicated that the rate of dna strand breaks increased with the increase of - rays dose. however, when the concentration of lexa protein from 0. 1 g / ml to 10 g / ml, it still showed no protective effect on dna strand breaks, then lexa protein of deinococcus radiodurans was n ' t radioprotector

    本研究採用了dna解旋熒光法檢測由射線所致的dna鏈斷裂的損傷程度以及抗輻射菌lexa蛋白的輻射防護作用，在輻照前加入不同濃度的lexa蛋白，檢測dna鏈的斷裂，結果表明dna鏈的斷裂程度隨著射線輻照劑量的增加而增強，抗輻射菌lexa蛋白對射線所致dna鏈斷裂的修復不起作用，進一步說明抗輻射菌lexa蛋白不起輻射防護作用。
  11. " nobody knows about the dominican remains. because they have n ' t yet allowed dna analysis, " rickards told reuters

    他說： 「如果分析結果證明哥倫布並非熱那亞人，那麼我們會授予他榮譽市民的稱號。 」
  12. In this article, the author amplified dna sequences encoding the mature peptides of human intact and truncated igf - 1 gene from human genomic dna by the new way of alternative pcr established and named as exons - piecing together method by this laboratory. the igf - 1 and des ( l - 3 ) igf - l gene were cloned in pgem - t vector and then subcloned in expression plasmid pgex - 3x. two recombinant constructs known as pgex - 3x - igf - l and pgex - 3x - des ( l - 3 ) igf - l were transformed to e. coli dh5 a respectively

    我們利用外顯子拼接法從人的基因組dna中擴增了編碼完整型及截短型人igf - 1基因成熟肽的dna片段，並將其克隆至pgem - t載體中；經測序證明正確后，又構建了表達載體pgex - 3x - igf - 1及pgex - 3x - des （ 1 - 3 ） igf - 1 ，在大腸桿菌中進行了表達研究。
  13. Because dna vaccine can not only induce protective humoral immunity but also induce strong t cell toxic response, its application on parasital diseases prevention is becoming more and more popular and studies on this area is gradually deepening. dna vaccine has been considered to be a potentially promising approach, which certainly throwed lights on t. s prevention

    它不但可以產生保護性體液免疫，並且可以誘發強烈的細胞毒性t細胞反應，因此普遍認為， dna疫苗極具發展潛力，它的問世將為免疫預防旋毛蟲病提供新的途徑。
  14. Hovever, the gene encoding the mature papain peptide was amplified using pcr from genomic dna extracted mid - development carica papaya fruit. about 98. 8 % of cdna nucleotide sequence reported in literature were homologous to the responding sequences of our study. there are three introns in the gene, in which the content of a and t is 86. 0 %, 79. 5 % and 90. 6 % respectively

    同時本研究以木瓜基因組dna為模板，通過pcr反應獲得了編碼木瓜蛋白酶成熟多肽部分的核苷酸序列，序列分析表明該基因含有三個內含子，其長度分別為157bp 、 266bp 、 88bp ， at含量分別為86 . 0 ， 79 . 5 、 90 . 6 。
  15. Pcr analysis of resistant seedlings with nptii gene primers showed that 6 out of 12 seedlings detected had the 700bp fragment specific to the plasmid pig121, indicating that t - dna had been integrated into the genome of sweet cherry

    L ，負壓的適宜處理是lmmxio次。 6 、通過p r擴增，初步證明證明外源基回己轉入甜櫻桃。
  16. Insertional mutagenesis using t - dna, transposons and plasmids axe commonly methods to creating a mutant library. here we summarize these strategies as well as the progress in the functional genomes research

    本文分別介紹三種方法的原理及其在功能基因組學研究中的應用和研究進展。
  17. 2. an anther specific chimaeric male sterile gene expression box with a enhanced promoter ( ta29 ) driving coda gene was constructed and the expression box was inserted into binary vector p3301 that contains a l - phosphinothricin ( ppt ) - resistant selective marker gene and - glucuronidase ( gus ) reporter gene in t - dna region

    以增強的ta29啟動子驅動克隆的coda基因，構建成花藥特異性嵌合基因表達盒；將此表達盒插入雙元載體p3301 ，構建成以ppt抗性基因為選擇標記，以gus為報告基因的植物表達載體。
  18. Mature embryo - derived calli of japonica rice ( oryza sativa l. ) cultivars nipponbare were transformed using agrobacterium tumefaciens strain agl1 carrying a binary vector pcas04 harboring the marker gene, neomycin phosphotransferase gene ( nptii ), driven by a promoter from the ubiquitin gene in maize, a promoterless p - glucuronidase gene near to the left border of t - dna for trapping gene and a strong promoter, rice actin - gb promoter, near to the right border of t - dna as activation tagging. in this system, co - cultivation was simplified, special selection stages and pre - regeneration stage were omitted, the whole process was almost under continuous light at 30 ? except co - cultivation and transgenic plants began to generate only after 7 weeks calli were induced

    在一步轉化系統中，光照高溫條件下培養的水稻愈傷組織從誘導開始經過4周時間就可以達到轉化實驗的要求，並且簡化、優化了整個共培養過程，省去了一篩、二篩和預分化步驟，只用7周的時間就可以初步得到再生轉化植株；共191塊愈傷組織得到125塊抗性愈傷組織，轉化頻率達到65 . 4 ，最後共得到99棵獨立來源的再生植株，抗性愈傷組織再生頻率達到79 . 2 。
  19. A single step transformation system for the generation of a large - scale t - dna insertional mutant population of rice was developed

    基於水稻大規模t - dna插入突變體庫的建立，我們發展了一個簡單、快速、高效的農桿菌介導的一步轉化系統。
  20. Totally 99 transgenic rice plants from 125 resistant calli of 191 calli were obtained and pcr assay showed that 80. 3 % of them were positive. the result of southern blotting analysis for primary plants revealed that each transgenic plant contained a average of 2. 5 copy of t - dna

    對抗性植株進行pcr擴增檢測，結果表明有80 . 3的抗性植株為陽性植株； southern雜交結果進一步證明了質粒的t - dna已經整合到水稻的基因組中，拷貝數為1 4個，平均每棵轉化植株有2 . 5個拷貝。
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