t-protein 中文意思是什麼

t-protein 解釋
t-蛋白
  • t : 中世紀羅馬數字的160。
  • protein : n. 【化學】朊，蛋白（質）。
  1. The total rna was isolated from pokeweed ( phytolacca americana ) leaves using the method of guanidine isothiocyante and used as template to amplify the total length and deleted mutant pokeweed antiviral protein ( pap ) gene by rt - pcr and then the pap gene was cloned into pgem - t vector. the sequencing results showed that pap gene had 99. 9 % identity comparing with the pap gene nucleotide sequence reported by lin et al ( 1991 ). the iptg - inducible expression vector containing the pap gene was constructed and transferred into e. coli bl21 ( de3 ) - plyss

    將缺失型pap基因克隆到植物表達載體pbi121中，通過液氮冷凍法將重組質粒轉入農桿菌lba4404細胞中，然後採用葉盤法，在該農桿菌的介導下將pap基因導入普通煙草中，經過卡那黴素抗性篩選，最後獲得了轉pap基因的工程煙草植株，摩擦接種煙草花葉病毒（ tmv ） ，與非轉基因煙草相比，能夠推遲癥狀表現達2月之久，說明pap基因能夠在其它植物體內產生有活性的高抗病毒的蛋白質。
  2. Results : after cryofixation, basement membranes of skeletal muscle consisted of only one electron dense layer, t tubules were round, core cylinders were observed in terminal cisternae and there were thread - like protein particles on the membranes of terminal cisternae

    化學固定后，縫匠肌基膜由兩層組成：一層電子密度低，另一層電子密度高；橫小管為扁平狀或啞鈴狀；終池內僅有一些散在的電子密度高的顆粒，終池膜上有幾個腳狀突起伸向橫小管。
  3. 17 - hsd8 / pcdna3. 1 recombinant protein expressed in hek 293 cells and it can slightly interconvert estrone to estradiol. moreover, it can catalyze the conversion from testosterone to androstenedione. however, 17 - hsd3 did n ' t show any conversion to all the substrates checked in this study

    而17p一hsds轉染hek293細胞表達的17p一hsds / pcdna3 . 1重組蛋白能微弱地催化el和ez之間的相互轉化，同時也能催化從t到a的氧化反應。
  4. In this paper, first strand cdna of 3abc gene was synthesized using template rna extracted from cells infected with fmdv. the complete 3abc gene about isoobp was amplified by pcr and ligated into pgem - t easy vector. after transforming e. coli dh5 a, ampicillin resistant colonies were isolated and plasmid dna was prepared and analyzed by restriction analysis and pcr. presence of the full length 3abc gene was verified by nucleotide sequence analysis and the plasmid containing the expected sequence was named as pgem - 3abc. comparing the aquired sequence of 3abc with that of reference strains, the homology is more than 99 percent. the pgem - 3abc was digested with sal i and bgl ii and ligated into xho i and bgl ii - digested expression vector ptriex - 4 neo. lt was identified by restriction analysis and pcr and sequencing that this fragment had a 17bp deletion hi the nucleotide sequence 708bp of 3abc gene, which happened to form a terminator codon behind 3ab gene, but it contained the complete open reading frame ( orf ) of 3ab gene. positive clones were selected and induced with lmmol / l isopropyl - d - galactoside ( iptg ), bacteria were detected by sds - page and western blotting after properly treated. the results showed that the 3ab gene expressed successfully in e. coli and 33. 5ku fusion protein can be recognized by the positive bovine serum of fmdv. the amount of target protein is over 26 % of the total bacteria protein by gel thin layer scanning analysis

    擴增產物連接到pgem - teasy載體中，轉化大腸桿菌dh5菌株，篩選氨芐青霉素抗性菌落，提取質粒經酶切鑒定、 pcr分析以及確證性測序證明，所克隆的1500bp左右的片段含有完整的3abc基因，與國外參考序列相比，同源性在99以上。將重組質粒pgem - 3abc和表達載體ptriex - 4neo分別用sal和bgl與xho和bgl消化后，亞克隆3abc基因至原核表達載體ptriex - 4neo中，通過酶切鑒定、 pcr擴增以及序列分析，發現克隆到ptriex - 4neo載體上的片段於3abc基因708bp處出現了17bp的缺失，碰巧在3ab基因后形成一終止密碼子，但3ab基因的閱讀框架完整，選出含有3ab基因完整閱讀框架的陽性克隆，用iptg誘導表達，收集菌液進行sds - page電泳、 westernblotting分析，結果表明， 3ab基因在大腸桿菌中成功表達，其表達產物為分子量33 . 5ku的融合蛋白，並能被口蹄疫病毒陽性血清識別。經薄層掃描分析，表達量占總蛋白量的26以上。
  5. Inspection for the biological activities of recombination protein of ? ctla4 extracellular domain in vitro : we investigated the role of ctla4e in t cell proliferation of mlr with 3h - tdr incorporation

    重組人ctla4胞外區蛋白體內生物學活性研究： l ）觀察ctla4胞外區蛋白在小鼠變應性鼻炎中的作用。
  6. However, kit protein doesn " t exist in oogenesis after birth. there are also c - myc positive protein granules in the spermatomeres from metaphase i to telophase ii, in the spermatozoa and in the secretory cells of the spermatheca. c - myc protein expresses not in the normal follicles, but in the follicular cells of the apoptotic ones

    Myc在處于減數分裂的中期至末期的精母細胞的細胞質或細胞核內呈陽性表達，在減數分裂后形成的精細胞和受精囊囊壁的上皮細胞的細胞質內也有陽性表達：但在出生后的卵子正常發生過程中無陽性表達，然而在凋亡卵泡的濾泡細胞的細胞質內有陽性表達。
  7. Containing many t cell epi - positions and neutralizing antibody epi - positions, the core protein region and envelope protein 2 of hepatitis c virus ( hcv ) structural protein may induce protective immunity responsion in body

    丙型肝炎病毒（ hepatitiscvirus ， hcv ）結構蛋白的核心蛋白（ c ）和包膜蛋白2 （ e2 ）含有多個t細胞表位和中和抗體表位，可誘導機體產生保護性免疫應答。
  8. This modification includes : ( 1 ) selecting two important molecules as candidates, ( 2 ) choosing a promiscuous t - cell epitope, and two b - cell epitopes or conserved amino acid sequences from the two important molecules, ( 3 ) connecting them adequately through analysis by the molecule designing software. therefore, the synthetic new antigen may interfere with the process of fertilization by multiple ways and its contraceptive effects may be enhancing. based on the molecule designing methods, the b - lymphocyte cell epitope of sperm / testis specific protein sp17 and cyritestin which interfere with fertilization in mouse, as well as the promiscuous th cell epitope of the ribonuclease ( rnase ) in bovine were selected

    本研究以蛋白質分子設計的理論和方法研究避孕疫苗，將sp17和cyritestin關鍵表位和牛核糖核酸酶非選擇性th細胞表位合理組合，獲得新抗原- 35肽序列；並在合成、純化後分別與弗氏佐劑、免疫刺激復合物（ iscoms ）混合后免疫不同遺傳背景的雌性小鼠，觀察血清和生殖道內的特異性抗體滴度的動態變化、生育力的改變以及免疫后小鼠重要臟器的組織病理學改變：以及在ivf下，新抗原的特異性抗血清對精卵相互作用的影響及抗原在精子表面的特異性定位。
  9. Scolopendra multilan is a common species belonging to scolopendromorpha, chilopoda. the sequence of scolopendra multilan mtdna determined is 11700 bp in length, about 70 % of the complete sequence. the sequenced portion contains 2 rrna genes, 1 a + t - rich region, 8 protein - coding genes and 18 trna genes

    本文對少棘蜈蚣的線粒體基因組進行了研究，已經測定的序列長11700bp ，約為全長序列的70 ，包含了2個rrna基因、 1個at富含區、 8個蛋白基因、 18個trna基因。
  10. Now it has been one of the most important aquatic products in the freshwater cultivation. however, this prawn ca n ' t survive at a water temperature lower than 14c, which has seriously limited its cultivation expanding. in order to obta in a new breed of this prawn with increased cold - resistance, we investigated the cloning of a synthetic gene ( sbwafp ) based on the primary sequence of the mature spruce budworm ( choristoneura fumiferana ) antifreeze protein ( sbwafp ) and the integration of sbwafp into the embryo genomes of giant freshwater prawn by spermatophore - microinjection ( smi ), a sperm - mediated gene transfer technique

    本研究的特色和創新之處在於，針對羅氏沼蝦不耐低溫，但體型相對較大，精莢明顯的特點，首次將目前已知具有最強抗凍活性的雲杉卷葉蛾（ sprucebudworm ， choristoneurafumiferana ）抗凍蛋白（ sbwafp ）基因（ sbwafp ） ，通過精子介導的轉基因技術整合到羅氏沼蝦的胚胎中，以期培育出耐低溫的羅氏沼蝦新品系。
  11. The quest continues for plasminogen activators with higher potency, specific thrombolytic activity, fibrin selectivity and longer half - life time. the recent progress in the protein engineering of plasminogen activators, including t - pa, u - pa, streptokinase, staphylokinase and other novel plasminogen activators, was presented in this article

    一些pa突變體及新型溶栓劑，如k2p tnk - pa和star等的臨床實驗結果表明它們在延長體內半壽期增強對血纖維蛋白選擇性和溶栓效率等方面有較大的改進。
  12. Objective to investigate the effects of total enteral nutrition ( ten ) vs. total parenteral nutrition ( tpn ) on protein metabolism, t - lymphocyte subsets and immune function of patient with major injuries

    目的觀察創傷后早期腸內和腸外營養對機體免疫和代謝功能的影響。
  13. Traditional nutritional protein markers ( albumin, transferrin and prealbumin ), immunoglobulin, t - lym - phocyte subsets, pha - stimulated lymphocyte proliferation, natural killer cell activity and nitrogen balance were measured before and after nutritional support

    結果較之腸外營養，腸內營養組營養前後前白蛋白變化明顯，淋巴細胞增殖率增加顯著。
  14. To investigate the mechanism of trefoil factor 3 on the gastric intestine epithelial restitution, the recombinant human trefoil factor 3 was added to human colonic tumor cell and the proliferation effect was examined by mtt assay. the recombinant protein didn t promote the proliferation of the hct cells at low density of 0. 010. 05 g l and only has weakly proliferation effect at density of 0. 10. 2 g l. 1 g l of the recombinant protein could significantly promote the cell migration of hct cells when added to the monolayers cells

    將重組人三葉因子3 trefoil factor 3 ， tff3作用於人結腸腫瘤細胞，研究重組蛋白對細胞增殖的影響，結果發現該蛋白在較低的濃度1050 mg l下對細胞的增殖基本沒有影響，在100200 mg l濃度下該蛋白對細胞僅有微弱的刺激作用，提高濃度對細胞增殖作用沒有改變。同時研究了tff3對損傷的單層結腸腫瘤細胞遷移的影響，發現tff3對細胞有明顯的促進遷移作用。
  15. Surprisingly, in both of archaebacteria and chloroplast, ftsz is the main component in the process of division but it is n ' t effective in mitochodria division, instead is a dynamin protein

    此外ftsz還是一種400個殘基的保守蛋白， ftsz與真核tubulin在結構、功能和進化上有一定的相似性及同源性，都具gtpase結構域，屬于g蛋白家族。
  16. After the recombinant plasmid pcdna3. 1 / ts87 was identified by digestion of hindlll and bamh i, it transformed into cos7 by lipofectamine. expression product was identified by immunohistochemical method, sds - page and western - blot. the immunocytochemistry result has shown that specific brown - staining grains were found in the cytoplasm of cells transformed by recombinant plasmid versus not seen in cells transformed by pcdna3. 1 or normal cells ; the sds - page result has revealed that a band about 3 8kb was found in cell lysis transformed by recombinant plasmid versus not in cells transformed by pcdnas. l or normal cells ; the western - blot result has showed that only the band about 38kd was recognized by sera from rabbit infected by t. s artificially and sera from rabbit immunized with soluble antigen of t. s and with protein expressed by ts87 gene and by a monoclonal antibody of t. s

    通過細胞的免疫組化，細胞裂解物的sds - page電泳， westem - blot分析檢測目的基因的表達情況。免疫組化結果顯示：重組質粒轉染的細胞質中有棕褐色顆粒，而空載體轉染細胞及正常細胞無此現象；細胞裂解物sds - page電泳結果顯示：只有重組質粒轉染的細胞在約38kd處有明顯的蛋白帶，這與理論計算的ts87基因表達蛋白的分子量為38kd基本一致； western - blot分析結果顯示：約38kd的蛋白帶能夠分別被旋毛蟲感染兔血清，成蟲蟲體可溶性抗原免疫兔血清， ts87基因原核表達蛋白免疫兔血清（ ts87血清）以及一株具保護性的旋毛蟲單抗特異識別。
  17. ? as same as most other schizothoracinae fishes, qinghai - lake nakked car ps may be tetraploid that already were diploidization ; ? qinghai - lake nakked carps arose from some primitive barbinae fishes, which gradually became special population adapting to high altitude environment by involvement and natural selection, on protein electrophoresis, out of 20 protein loci among 132 individuals in the present experiment, only t mdh > amy est and pod showed polymorphism

    在蛋白水平上，共檢測了132尾裸鯉的20個遺傳座位，僅發現tf ， mdh 、 amy 、 est和pod五個座位表現出多態性，其中， tf 、 mdh 、 amy和est四個座位均有一對等位基因；而pod座位表現出復雜的電泳圖譜，未進行深入分析。
  18. In this study, dna strand breaks in jm109 cells caused by - rays and the protective effect of lexa protein of deinococcus radiodurans have been studied by fluorometric assay of dna unwinding., the results indicated that the rate of dna strand breaks increased with the increase of - rays dose. however, when the concentration of lexa protein from 0. 1 g / ml to 10 g / ml, it still showed no protective effect on dna strand breaks, then lexa protein of deinococcus radiodurans was n ' t radioprotector

    本研究採用了dna解旋熒光法檢測由射線所致的dna鏈斷裂的損傷程度以及抗輻射菌lexa蛋白的輻射防護作用，在輻照前加入不同濃度的lexa蛋白，檢測dna鏈的斷裂，結果表明dna鏈的斷裂程度隨著射線輻照劑量的增加而增強，抗輻射菌lexa蛋白對射線所致dna鏈斷裂的修復不起作用，進一步說明抗輻射菌lexa蛋白不起輻射防護作用。
  19. The effect of 20 g / 100 g diet of lactalbumin ( l ), casein ( c ), soy ( s ) and wheat ( w ) protein on the immune responsiveness of c3h / hen mice has been investigated by measuring the humoral immune response to the t cell - independent antigen, tnp - ficoll

    摘要：本研究測量體液對t細胞抗原的免疫反應，探討不同的蛋白補充劑（包括乳白蛋白、酪蛋白、大豆蛋白及小麥蛋白）飼料（ 20克蛋白/ 100克飼料）對小鼠免疫反應的效果。
  20. High efficient expression and purification of the escherichia coli t - protein

    蛋白的高效表達和分離純化
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