target gene 中文意思是什麼
target gene
解釋
目的基因-
Screening target ' gene of artemisinin antimalarials using drug - western from cdna expressing library : 12 b - deoxoartemisinyl - ( 4 ' - oxyacetic acid ) phenyl ether was linked to bsa by using of edc cross ! inker and the product acted as drug - probe
對重組pmd一18一t克隆載體及pqe一30表達載體雙酶切，提取tctp基因和pqe一30空載體並使二者重組，然後轉化m15 ，挑取陽 -
The target gene was then subcloned into plasmid vector and induced by iptg for its expression. after that, the recombinant protein was purified and used for the identification and characterization of its immunogenicity. the effect of the induced specific ige antibody on the hepatic granuloma formation and fibrosis after challenge infection with schistosome cercariae was evaluated
Niptg誘導表達，產物沉澱中於約45kda處顯見高合蛋白表達帶， westernblot分析表明，該蛋白帶可被篩庫血清中特異性lge抗體識別；而載體本身表達的26gst蛋白帶則否。 -
According to these problems, we adopt to the method of mending material, optimize to fermentation media and partly ferment condition. finally, we excogitate a kind of fermentation technology that is suitable for target gene efficiency expressed and is advantageous of product purified. with the plasmid pbv220 - ifnr, pbv220 - hgfa, pbv220 - hgfb, pbv220 - hpk5 that expresses serve as the model, adopting the biostat - c15l of b. braun company, utilize the method of mending material to ferment, through optimization fermentation media and optimization partly ferment condition ( ventilate quantity, stir speed, mend material speed ), eventually establishment a kind of fermentation technology that is suitable for target gene efficiency expressed and is advantageous of product purified
以我室構建並穩定表達的重組質粒pbv220 - - ifn 、 pbv220 - hgf 、 pbv220 - hgf 、 pbv220 - hpk5為模型，分別從不同的表達宿主菌中篩選出一種適合大規模生產的菌種bl21 （ de3 ） ，該工程菌株連續傳代100代表達質粒不丟失，表達量穩定；採用b . braun公司的biostat - c15l自控發酵罐，運用分批補料技術分別進行四種工程菌的高密度發酵，通過優化工程菌發酵的培養基配方及優化部分發酵條件（通氣量、攪拌速度、補料速度） ，最終建立一種適于目的基因高效表達的高密度發酵工藝模式。 -
These results suggested that dgp1 could drive target gene expressed mainly in guard cells when plant is subjected to drought stress
這些結果證明，在植物遭遇乾旱脅迫時， dgp1啟動子可以驅動目的基因在保衛細胞中特異性表達。 -
Detection of transgenic plants. the pcr assay of kan - resistant plants showed that the target gene had been integrated into tobacco accompanying with npt ii gene
轉基因pcr檢測pcr擴增npt11基因，證明thaumatin基因己導入煙草中，轉化率為引 -
Methods : ( 1 ) the segregation of foreign target gene in the t1 by histochmical gus assays ; ( 2 ) identification of pure line from transgenic tomatoes ( tl ) through examining gus expression in pollens in conjunction with pcr analysis of marker gene ( npt ) ; ( 3 ) the transcript levels of leetrl or leetr2 in anti - sense transgenic plants ; ( 4 ) the phenotypes of the transgenic plants in tomato during whole life cycle under ethylene - treated and non - treated conditions
本研究以反義乙烯受體leetr1 ， leetr2基因番茄t _ 0代種子為實驗材料，利用gus基因表達研究外源基因的遺傳規律，並藉助于pcr技術對目的和標記基因的鑒定獲得轉基因t _ 1代材料。利用gus基因在t1花粉中的表達鑒定獲得轉基因純合植株。研究了轉基因後代的生長發育模式、對外源乙烯敏感性，以及靶基因的表達特性，初步探明了它們在乙烯受體系統中的功能。 -
The positive colonies that grew on the ampicillin ( amp ) plate ( lb agar medium contaning 100 g / ml amp ) were screened and identified. sds - page and western blot analysis were performed to study the expression profiles of target gene cein in e. coli
從轉化的平板中篩選出陽性重組子，進行不同iptg濃度和不同誘導時間的表達研究，並利用sds - page電泳和westernblotting蛋白質印跡技術對外源基因cei _ （ 12 ）在大腸桿菌e -
We then consider cell - based peripheral clock models for target gene discovery, chemical biology and future drug discovery
於是我們認為以細胞為基礎的外圍時鐘適用於基因的發現，化學生物學和未來麻醉藥的發明的目標。 -
They contained an antisense constructed for the spruce gene encoding ccr ( cinnamoyl alcohol dehydrogenase ), an enzyme of monolignol synthesis. the antisense rna method is a technique for reducing the expression of a resident target gene. the transgenic sublines were produced by particle bombardment at the dept of forest genetics, swedish university of agricultural sciences
本項目研究的目的就是採用瑞典農業大學構建的反義ccr基因轉導的挪威雲杉細胞愈傷組織，通過誘導產生再生植株，並檢測證實該基因是否表達及其它相關基因的表達狀況，為培育出低木質素的轉基因挪威雲杉新品種奠定物質和理論基礎。 -
Salmonella typhimuriwn, one of the invasive bacterial species, can be attenuated without loss of invasiveness and thus used for delivery of eukaryotic expression vectors into host cells in vivo. the recombinant plasmid containing the target gene is released inside the host cells and gain entry into the nucleus, resulting in expression of encoded antigens and subsequent induction of humoral and cellular immune responses
沙門氏菌（ salmonellatyphimurium ）是一種較為常見的侵襲性胞內菌，通過基因工程方法減毒后對宿主致病性顯著降低，但仍保留良好的侵襲力，可直接將真核表達質粒攜帶進入動物細胞內表達相應的蛋白而誘導特異性的免疫應答反應。 -
Results showed that : ( 1 ) in general, the segregation ratios of target gene in some tl lines are conformed to 3 : 1, however, some are not possibly due to gene silence or missing in the self - pollinated progeny ; ( 2 ) two homozygous plants were identified from 10 putative transgenic plants
主要結果如下： （ 1 ）我們對外源基因在番茄體內的遺傳規律研究表明，在大多數情況下，單拷貝插入外源基因將導致轉基因後代按孟德爾單顯性基因3 1規律分離，但是部分轉化系比例偏低，可能發生了基因丟失或者沉默。 -
If we study on the phylogenetic relationship in intra - genus or intra - species using gapdh as a target gene, it may be make a discrepancy
對于距現今較近的動物，用其作為近親緣關系的屬內種間的標記基因，可能產生偏差。 -
The most efficient regulation of gene occurs at transcription level by regulating the interaction between transcription factors and upstream regulation sequence. thus, to investigate promoter of a target gene will be helpful to predicate the principle of molecule regulation, biological function of molecule and even involving pathogenesis of some diseases
轉錄水平是調控蛋白質表達效率最高的環節，通過影響相應的轉錄因子與啟動子和上游調控序列的相互作用調控目的基因的表達，因此研究基因的啟動子對于了解基因的表達調控規律、闡明分子的結構和生物學功能乃至疾病的發生都有重要的意義。 -
Expression of target gene in mesenchymal stem cells after transfection of basic fibroblast growth factor gene
堿性成纖維細胞生長因子基因轉染大鼠骨髓間充質幹細胞后目的基因的表達 -
Antisense nucleic acid technique is a method using single - stranded complementary sequences with specificity assigned by watson - crick base - pairing targeted specific messenger rna or dna to block expression of target gene
反義核酸技術是根據堿基互補原理，使用與目標靶的遺傳物質（ mrna或dna ）特定互補的核酸片段封閉基因表達的技術方法。 -
This specific database will provide useful information for analysis of substantial equivalence of gm foods with its traditional counterparts in two aspects : ( 1 ) providing information on whether there is any toxin or anti - nutrient proteins existed in traditional crops ; ( 2 ) providing information on homology comparison of ammo acid sequence coding by target gene ( s ) with known toxin proteins or anti - nutrient factors to predict whether there is any possible risk for transgenic crops and its products
建立的數據庫可為轉基因食品的實質等同性分析提供兩方面的信息： （ 1 ）傳統食品中存在何種毒蛋白和抗營養因子蛋白； （ 2 ）已知毒蛋白和抗營養因子蛋白的氨基酸序列，可用於目的基因的同源性比較和分析預測。 -
The homologious comparison proved the cloned gene had 96 % homology to the sequence of the omp gene, and the alignment of the amino acid sequence was 98 %. the recombinant plasmid was constructed with the target gene and the expressing vector pgex - 4t - l and then was transformed into e. coli bl21 ( de3 ) the fusion protein was expressed under the iptg inducing condition, and exhibited about 62kda in size, very close to the predicted molecular weight of gst - momp. furthermore, the fusion protein was specifically recognized by anti - serum which raised against the major outer membrane protein of ahl316
Sds - page電泳分析顯示誘導表達的基因產物分子量約為62kda ，與預測的gst -外膜蛋白重組融合蛋白的分子量極為相似， western - blot進一步證實，表達產物能被嗜水氣單胞菌l316主要外膜蛋白特異性抗血清所識別，產生明顯的染色條帶，說明所表達的基因產物與天然的外膜蛋白抗原性一致。 -
Target gene was cloned into the procaryotic fusion expression vector pet28a ( + ), then subcloned into the eucaryote expression vector pcdna3. 1 ( + ) after sequence analysis
將halr基因克隆到原核融合表達載體pet28a （ + ）上，序列分析后亞克隆到真核表達載體pcdan3 . 1 （ + ）上。 -
Introduced infected by the turnip mosaic virus. there are many useful objective genes can be used in vegetable genetic transformation, but the research work of chinese cabbage genetic transformation is little. the reaserch on transforming chinese cabbage using agrobacterium - med ated method has n ' t been report at home and abroad. ln the test, tumv - cp gene was transferred into chinese cabbage ( brassica campestris l. ssp. pekinensis ) via agrobacterium - mediated method. a high effective regeneration and genetic transformatin system has been established, the detection by the method of molecular biology, has proved that the regenerative plants are transgenic plants and the target genes have been expressed transgenic plants partly. meanwhile transgenic progenies were traced and investigated so that heredity, stability and expression of target gene were researched. the virus resistant, stable plants were expected to obt ain so that theoretical base can be established for chinese cabbage breeding by gene engineering
利用農桿菌介導法轉化大白菜抗病基因的研究工作在國內外未見報道。本課試驗採用農桿菌介導法將tumv - cp基因導入大白菜中，建立了高效的大白菜離體再生、遺傳轉化體系，並對轉基因植株進行分子生物學檢測，證實得到的再生植株為轉基因植株，目的基因已在部分植株上表達。同時，對轉基因植株的後代進行檢測，分析該基因所控制性狀的遺傳穩定性以及基因表達情況，為大白菜基因工程抗病育種提供理論依據。 -
A surface antigen hemagglutinin - neuraminidase ( hn ) gene of the ndv b95 strain, was selected to study as target gene. referred to the reported sequence, rwo primers were designed and synthesized. hn gene of ndv b95 strain was amplified by rt - pcr, the pcr products were checked by agrose gel electrophoresis and purified by agrose gel fraction method
為此，從澳大利亞某大學獸醫病理系引進一株ndv熱穩定性天然弱毒b95株，並選取其表面抗原血凝素?神經氨酸酶的編碼基因為目的基因，根據國外已發表的序列，設計一對引物，利用rt - pcr技術擴增出了ndvb95株hn基因。
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