targeting sequence 中文意思是什麼
targeting sequence
解釋
導肽-
This sequence emergences fourteen times from 1000 ests library indicts that it is a middle affluently gene in cdna library. the cdna of 634 basepairs contains an open reading frame of 339 nucleotides encoding a novel nonspecific lipid transfer protein. the first 23 amino acids constitute the putative signal peptide, characteristic for targeting to the secretory pathway
測得th - nsltp序列全長為634bp ，含有一個非特異性脂轉移蛋白與植物耐逆性的相關性研究編碼112個氨基酸的閱讀框架， n端的23個氨基酸組成一段信號肽序列，表明它可能和分泌有關。 -
According to the gene sequence and secondary structure of hcv ns5b, we design the sirnas targeting ns5b gene following with the requirement for sirnas design from tuschl et. al and synthesize it from dharmacon company ; hepg2 cell stably expressing ns5b - egfp protein was trasfected by synthesized sirnas with electroportion, the non - transfected cell and non - specific sirnas transfected cell are c onsidered as control group ; inhibitory effect of sirnas was investigated by fluorescence microscope with dapi dyeing and by semi - quantitative rt - pcr
然後根據dsrna設計原則，結合nssb基因的序列特徵，藉助生物信息學軟體設計了針對nssb基因的sirnas ，並交由公司化學合成；電穿孔法轉染上述穩定轉染的細胞克隆，同時分別以非特異的sirnas轉染組和空白轉染組為對照， dapi染色后通過熒光顯微鏡和內標化rtpcr檢測，初步證實了化學合成的sirnas可以特異阻斷nssb基因的表達。 -
The gene targeting vector was planning to be constructed by using thrombopoietin gene ( tpo ) gene as purpose gene, as 1 - casein gene as targeting gene, the 5 " sequence of - casein gene as promotor
以綿羊-酪蛋白基因的5調控區為啟動子，人血小板生成素基因為目的基因，奶牛s1 -酪蛋白基因為靶向基因。 -
And the fusion protein could been purified quickly by thiobond ? resin. this was key step to produce cmiv largely by fermentation. meanwhile, the structure of the mutation ii of cmiv showed a new way to design peptide of cecropin which have the targeting sequence to tumor cell
以上研究的意義： 1 、初步解決了抗菌肽cmiv突變體在大腸桿菌中高效可溶性表達的問題，為進一步大量生產cmiv基因突變體打下基礎； 2 、為進一步設計具有特異導向性的抗菌抗腫瘤cmiv突變體基因和表達以及以後進行動植物轉化打下基礎。 -
3. the mechanism of dna integration mediated by homologous recombination in chloroplast transformation was tentatively explored. we isolated two chloroplast dna fragments from brassica napus and used them as targeting sequence to integr
成功地通過h次轉化獲得整合併表達多基因的轉基因煙草，縮短了研究周期，對相關轉基因植物的研究有一定參考價值。 -
The targeting sequence should take
瞄準定位大約需要
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