template reaction 中文意思是什麼
template reaction
解釋
模板反應-
Establishing snupe for 2 y - snp loci, we understand that the suitable thermostable dn a polymerase and proportion of template and primer are the keys to reaction of snupe
通過建立2個y - snp的snupe反應，了解snupe反應的一般規律，揭示單堿基引物延伸反應的關鍵是選擇合適的dna聚合酶及合適的引物和模板的比例。 -
2. cloning of structural genes of bacillus subtilis bio operon diluted the genomic dna of bacillus subtilis as the template, long pcr product ( 10. 3kb ) and three salvage pcr products were separately gained by optimization of reaction conditions of pcr
枯草桿菌生物素操縱子基因的克隆將枯草桿菌基因組dna稀釋后，通過pcr反應條件的優化，分別擴增得到了生物素操縱子基因的長距離pcr產物（ 10 . 3kb ）和3個分段pcr產物。 -
The genomic dna of ginkgo extracted by ctab method is light brown in color. it is neither digested with restriction endonucleases nor acted as template for polymerase chain reaction ( pcr )
常規的ctab法提取的銀杏基因組dna呈黃色，不易被內切酶所消化，也不能作為pcr模板。 -
To isolate hfl -, hf2 gene encoding flavonoid - 3 ', 5 ' - hydroxylase ( f3 ', 5 ' h ) from petunia hybrida of the purple flower, we used a polymerase chain reaction ( pcr ) amplification using the genomic dna as a template. the result indicated that the hfl dna was 2797bp in total length and encoded 506 amino - acid, containing three extron and two intron ; hf2 dna was 2223bp in total length and encoded 509 amino - acid, containing three extron and two intron. comparison of the sequence with genbank databases revealed that hfl dna and hf2 dna shared significant nucleotides identity ( 98. 97 % and 99. 04 % respectively ) and amino - acid identity ( 99. 41 % and 99. 41 % respectively )
Hf1dna全長2797bp ，包括3個外顯子， 2個內含子，其中一個開放閱讀框架（ orf ）編碼506個氨基酸，與文獻報道的序列相比較，具有98 . 97核苷酸同源性與99 . 41氨基酸同源性： hf2dna全長2223bp ，包括3個外顯子， 2個內含子，其中一個開放閱讀框架（ orf ）編碼509個氨基酸，與文獻報道的核苷酸同源率為99 . 04以及氨基酸同源率為99 . 41 。 -
The cdna encoding growth hormone ( gh ) peptide was amplified by reverse transcription polymerase chain reaction ( rt - pcr ) method using isolated total rna as template
應用逆轉錄-聚合酶鏈式反應（ rt - pcr ）技術克隆得到編碼草魚生長激素（ cgh ）的基因cdna ，並定向克隆到puc18載體上。 -
In order to get small molecular g - protein rab3a, which serves to further investigate the interaction between rab3a and other proteins, we amplified the full coding region of rab3a cdna by polymerase chain reaction, using human placenta total cdna as template. the pcr products were recovered from gel electrophoresis and cloned into bamhi xhoi site of vector pyestrp2. the result of sequencing indicated that rab3a insertion fragment included its initiation and termination codons in 5 - and 3 - terminal, respectively
為了獲取全長的小分子g -蛋白rab3a ，以用於研究rab3a與其他蛋白相互作用關系，本實驗以人胎盤總cdna為模板， pcr擴增到人rab3a cdna全編碼區。產物回收后克隆于質粒pyestrp2的bamhi xhoi位點，測序結果表明，本實驗獲得的rab3a cdna包含了起始和終止密碼子。 -
The interaction between template and functional monomer was proved by uv spectrophotometry and based on the results, appropriate reaction solvent was selected
紫外分光光度法證明了模板分子與功能單體之間存在相互作用，並據此選擇了聚合反應時合適的溶劑。
分享友人