terminal column 中文意思是什麼
terminal column
解釋
末列-
The hwtx - i gene was chemically synthesized according to its known cdna sequence, the gene was inserted into vector ppic9k which contained aoxj promotor and the sequence of a secreting signal peptide - a - factor, the cloning ppic9k / hwtx - i was constructed and confirmed by two - step pcr and dna sequence analysis, then it was transformed into host strain gs115, a his + muts cell line was screened and multicopy transformants were screened by various g418 concentrations, the multicopy transformant was named gh1. gh1 was cultivated in flasks. after 6 days of induction by 0. 5 % methanol, the supernatant was checked by 16. 5 % tricine - sds page, which showed there was a band in the position of 3. 5 - 6. 1kd, then it was isolated and desalted by ultrofiltration followed by ion exchange of cm column, after reverse phase hplc of ci8 and vacuum drying, the purified rhwtx - 1 was obtained which was proved to be correct recombinant hwtx - i by tricine sds - page, maldi - tof mass spectrometry, amino acid composition analysis, the n - terminal amino acid sequence and its biological activity, the final field of the purified rhwtx - i was about 80mg / l, accounting for 23. 6 % of it total secretory proteins
將帶有hwtx -基因的ppic9k經blgii線性化后,轉化酵母宿主菌gs115原生質體后經篩選陽性克隆並經表型鑒定為his ~ + mut ~ s酵母菌,進一步用遺傳毒素g418篩選多拷貝的轉化菌株,命名為gh1 ;將gh1甲醇酵母菌用0 . 5的甲醇誘導表達,發酵上清經90飽和度的( nh _ 4 ) _ 2so _ 4沉澱, yw - 3 ( mwc03000 )的超濾膜超濾,再經cm陽離子交換, c _ ( 18 )反相hplc純化得到分子量為4kd左右的組分,其中4289 . 05的組分經質譜鑒定,氨基酸組成分析和序列測定為正確的表達產物,生物學活性表明其活性為天然毒素活性70 % ,表達量為80mg / l 。 -
Judgment of burning failures for a column outlead terminal of traction transformer in ss3 electric locomotive
柱引出線接頭燒損故障的判斷 -
Full - scale test of battened tubular column in new beiyun international airport terminal
新白雲國際機場航站樓鋼管綴板柱足尺試驗 -
After sequencing the vectors were transformed into e. coli dh5 a and recombinant fusion protein was expressed via induction of iptg. fusion protein coded by carboxyl terminal gene sequence was purified through glutathione agarose column
經sds page分析,在相對分于質量( mr )為74000和43000處,各有1條特異的蛋白帶。 -
Mechanical standardization of semiconductor devices - general rules for the preparation of outline drawings of surface mounted semiconductor device packages - design guide for 1, 50 mm, 1, 27 mm and 1, 00 mm pitch ball and column terminal packages
半導體裝置的機械標準化.表面安裝的半導體裝置封裝外形圖繪制的一般規則. 1 . 5mm 1 . 27mm和1 . 00mm樹脂球和引線端子封裝的設計指南 -
Mechanical standardization of semiconductor devices - part 6 - 2 : general rules for the preparation of outline drawings of surface mounted semiconductor device packages ; design guide for 1, 50 mm, 1, 27 mm and 1, 00 mm pitch ball and column terminal packages
半導體器件的機械標準化.第6 - 2部分:繪製表面安裝半導體器件封裝外形圖的一般規則. 1 . 5mm 1 . 27mm和1 . 00mm樹脂球和引線端子封裝的設計指南 -
Mechnical standardization of semiconductor devices - part 6 - 2 : general rules for the preparation of outline drawings of surface mounted semiconductor device packages - design guide for 1, 50 mm, 1, 27 mm and 1, 00 mm pitch ball and column terminal packages
半導體器件的機械標準化.第6 - 2部分:半導體器件包裝用表面安裝略圖制備的一般規則. 1 . 5 mm 1 . 27 mm和1 . 00 mm螺距球面和柱狀終端包的設計指南
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