terminal gene 中文意思是什麼
terminal gene
解釋
末端基因-
The fusion protein was bactericidal active against staphylococcus aureus. in present study, we will truncate the none channel - forming do main, then attach the agrd to the pore - forming region ( k544 - i626 ) to construct a new engineered multidqmain protein machine - compact engineered peptide targeting staphylococcus aureus. such engineered peptide was constructed by linking the gene of staphylococcal agrd pheromone with the gene of c - terminal ( 1626 ) of colicin la pore - forming region ( k544 - i626 ) with site - directed mutation
利用點突變方法將金黃色葡萄球菌信息素agrd （ i型， ystcdfim ）的基因引入到大腸菌素fa梭基端1626基因上，並將限制性內切酶sacl酶切位點基因分別引入到大腸菌素fa的p4和k544上，通過酶切、膠回收、連接獲得含大腸菌素ia水性孔道結構域和金黃色葡萄球菌信息素agrd基因的重組質粒。 -
Distribution and source of calcitonin gene - related peptide immunoreactive nerve terminal in prepuce of penis and frenulum of prepuce of rats
大鼠陰莖包皮及包皮系帶內降鈣素基因相關肽免疫陽性神經末梢的分佈與起源 -
The extracellular 100pl ( ecl1 ) and ecl2 was linked by disulfide bond to maintenanced the stability of the protein secondary structure. in recent years, we showed that ccr5 as a co - receptor could interact with hiv - 1 infect ion. ccr5 was paid closed attention to since it was cloned in 1996. the aim is to - obtain the sequence of first extracillular domain of 3 chemokine receptor 5 ( ccr5 ) n - terminal gene fragment with high level expression in e. coli and to prepare its specific antibody f ( ab ' ) ;, and its detected method
Ccr5具有g蛋白偶聯受體家族（ g - proteincoupledreceptors ， gpcrs ）所特有的7個跨膜區（ transmembrinedomains ， tm ） ，呈螺旋， tm的氨基酸有很高的保守性，膜外第一襻（ extracellularloop1 ， ecl1 ）和ecl2之間有二硫鍵相連，以維持蛋白質二級結構的穩定性。 -
The hwtx - i gene was chemically synthesized according to its known cdna sequence, the gene was inserted into vector ppic9k which contained aoxj promotor and the sequence of a secreting signal peptide - a - factor, the cloning ppic9k / hwtx - i was constructed and confirmed by two - step pcr and dna sequence analysis, then it was transformed into host strain gs115, a his + muts cell line was screened and multicopy transformants were screened by various g418 concentrations, the multicopy transformant was named gh1. gh1 was cultivated in flasks. after 6 days of induction by 0. 5 % methanol, the supernatant was checked by 16. 5 % tricine - sds page, which showed there was a band in the position of 3. 5 - 6. 1kd, then it was isolated and desalted by ultrofiltration followed by ion exchange of cm column, after reverse phase hplc of ci8 and vacuum drying, the purified rhwtx - 1 was obtained which was proved to be correct recombinant hwtx - i by tricine sds - page, maldi - tof mass spectrometry, amino acid composition analysis, the n - terminal amino acid sequence and its biological activity, the final field of the purified rhwtx - i was about 80mg / l, accounting for 23. 6 % of it total secretory proteins
將帶有hwtx -基因的ppic9k經blgii線性化后，轉化酵母宿主菌gs115原生質體后經篩選陽性克隆並經表型鑒定為his ~ + mut ~ s酵母菌，進一步用遺傳毒素g418篩選多拷貝的轉化菌株，命名為gh1 ；將gh1甲醇酵母菌用0 . 5的甲醇誘導表達，發酵上清經90飽和度的（ nh _ 4 ） _ 2so _ 4沉澱， yw - 3 （ mwc03000 ）的超濾膜超濾，再經cm陽離子交換， c _ （ 18 ）反相hplc純化得到分子量為4kd左右的組分，其中4289 . 05的組分經質譜鑒定，氨基酸組成分析和序列測定為正確的表達產物，生物學活性表明其活性為天然毒素活性70 % ，表達量為80mg / l 。 -
Both the mature genes of gloshedobin and gussurobin were cloned into the vector pet - 32a ( + ), strain bl21 ( de3 ), to study their expression in prokaryotic cell. the gene was expressed under t7 promoter with a fusion protein partner of thx. tag and a 6x his. tag at its n - terminal. having been induced by iptg for 4 hours, the recombinant enzyme was examined in the cytoplasm by sds - page analysis
將大連蛇島蝮蛇和長白山白眉蝮蛇毒類凝血酶基因分別克隆到大腸桿菌表達載體pet - 32 （ a ） +中，在t7啟動子下表達出融合蛋白，融合部分為硫氧還蛋白，位於類凝血酶基因上游，並在其n端帶6xhistag標簽以利於表達產物的分離純化，經熱激轉化至宿主菌bl21 （ de3 ）中， iptg誘導斗小時后收獲菌體。 -
An expression vector carrying a fragment encoding the amino - terminal part of an fr - 008 type i pks module, containing a keto - synthase ( ks ) and part of an acetyl - transferase ( at ) domain was constructed for trial expression of the extremely high g + c content ( 76 % ) pks gene in plant
為探索在植物中表達極高g + c含量的pks基因的可能性，構建了攜帶有編碼fr - 008型pks模塊氨基端部分的基因的表達質粒，包括一個酮基合酶（ ks ）和部分酰基轉移酶（ at ）活性結構域。 -
A pair of primers, based on the high homologous n - and c - terminal amino acids sequences of subtilisin of bacillus pumilus, were designed and used to amplify subtilisin gene from genomic dna of 4 different bacillus strains by polymerase chain reaction
Sds 、 edta和pmsf對酶活力有不同程度的抑制作用。以4株芽孢桿菌的總dna為模版，利用擴增短小芽孢桿菌堿性蛋白酶基因的引物對它們進行pcr擴增。 -
A pair of primers were designed according to the published sequence of gnrh2 ' s gene mrna and transporter gene with oligo version 4. 1 softwarre, they were annealed by their 3 ' terminal brief complementary base sequence to form small segment double chain, therery, they serve mutually as template and primer, and their extension were carried out to synthesize 90 bp " gnrh / trs
本研究根據genbank中已發表的人gnrh2基因mrna序列以及轉運肽（ transporter ， trs ）基因核苷酸序列，藉助oligo4 . 1設計了一對寡核苷酸引物，以引物3末端的短互補序列退火形成小段雙鏈，從而互為模板和引物，通過引導合成長達90bp的gnrh trs序列gnrh trs 。 -
Not only sequence information from the c - terminus of proteins and peptides is of especial interest in the investigation of n - terminally blocked proteins and peptides, but also c - terminal sequence analysis can facilitate the production of more specific probes for gene cloning
C端與n端一樣，在蛋白質分子結構分析中具有重要的地位，對其順序測定具有重要的意義，不僅可以對n端封閉的蛋白質進行序列測定，而且對基因克隆有指導意義。 -
A human lymphotoxin deletion gene fragment which lacking n - terminal 27 amino acid residues of the protein was pcr amplified using a pair of designed primers and cloned into expression vector pet36b ( + ) to construct a fusion protein with cbd tag, resulting a recombinant plasmid pet36b - lt 27. the recombinant plasmid was transformed into host e. coli bl21 ( de3 ) plyss
採用pcr的方法擴增出人淋巴毒素n端缺失27個氨基酸的缺失體基因片段，將此基因片段克隆至表達載體pet36b （ + ）上，與t7lac啟動子控制下的cbdtag構建成表達融合蛋白的重組質粒pe736b - lt 27 。 -
The n - terminal nucleotides 47 - 420 of the guangxi apmv - 1 isolates of different poultry species origin were amplified and sequenced. the alignment and phylogenetic analysis of the nucleotide sequences and deduced amino acid sequences of f gene of the guangxi isolates and other reference strains obtained from genbank were done
研究對雞、鵝、鴿三種禽源apmv刁廣西分離株f基因n與前段進行了rtpcr擴增及核茸酸序列測定，並用基因分析軟體dnastar進行分析並與已發表的其它參考毒株進行比較。 -
Major difference of f gene lies in no. 1 - no. 32 amino acid residues on n - terminal, which is the signal sequence of f protein and cut off short after the new peptide of f protein is combined with the membrane of vesicle. the sequence is not the composition of the f protein and the evolutronary force it bears is very small
氨基酸的差異主要集中在n端1 - 32殘基，該序列為f蛋白的信號序列，在f蛋白新生多肽與囊膜泡結合后不久被切下來，因此他不是功能性f蛋白的構成成分，所承受的進化強制就很小。 -
Sequence analysis showed highly similarity to other plants such as narcissus pseudonarcissus., and they all possess a short highly conserved n - terminal sequence encoding a dinucleotide cofactor binding site. thus it is a new member of phytoene desaturase gene family
由此可認為該番紅花八氫番茄紅素脫氫酶基因在結構上與其它植物八氫番茄紅素脫氫酶基因是同源的，是八氫番茄紅素脫氫酶基因家族中的一個新成員。 -
Hegf gene with his - tag at the end, which was derived from pet22 - egf, was in - frame fused to the carboxy - terminal of polyhedrin ( ph ) gene, which included the amino - terminal 116aa coding region. the polyhedrin - egf fusion gene ( named ph - egf ) was then cut out with ecorv and ecori, and was cloned between ecorv and ecori sites of pbacpak. 8 ( the result plasmid was named pbacph - egf and the ph - egf fusing gene was right under the control of ph promoter ). the pbacph - egf structure was verified with restriction enzymes digestion and pcr
從pet22 - egf質粒中分離出末端帶his - tag的egf基因，對位融合於多角體蛋白n端116個氨基酸基因序列的下游（命名為ph - egf ） ，並在兩段基因間設計了凝血酶xa蛋白酶切位點，經過酶切、測序等鑒定正確后，克隆至pbacpak8中，使ph - egf融合基因置於多角體蛋白（ polyhedrin ， ph ）基因啟動子控制之下，構建成重組轉移載體pbacph - egf 。 -
To avoid cross hybridzation due to the conservative myb - domain a fragment specific only for the c - terminal part of the gene was used as a probe. independent of the restrictase used for digestion single band was detected
實驗中為了避免由於保守的myb結構域而造成該基因和同類轉錄因於的雜交，所用探針僅用mxmybl基因c一端的特異性片段。 -
Major difference of hn proteins lies in no. 18 - no. 75 amino acid residues on n - terminal which includes three active sites of hn protein. the influence on biological action, caused by the difference of hn protein, is needed to study further. hn gene has only four glycosylation sites, which is 1 or 2 less on amount than that of other strains. so, this difference can be take on as a natural mark of ndv b95 strain furthermore, the fragment encoding hn gene was excised from the positive clone pgem - hn with sail and saci enzyme, and purified by agrose gel fraction method. then the fragment was subcloned into the pet - 28c expressing vector digested by sail and saci restriction enzyme
作者將正確的重組克隆質粒pgem - hn用saii 、 saci雙酶切，電泳回收目的片段，將其亞克隆到經saii 、 saci雙酶切處理的pet - 28c中，轉化大腸桿菌tgi感受態細胞，得到的轉化子經pcr鑒定和酶切分析，篩選出符合閱讀框的重組子，構建成重組表達質粒pet - hn ，並在大腸桿菌bl _ （ 21 ） （ de _ 3 ）宿主菌中成功地表達了含目的蛋白的融合蛋白，融合蛋白的分子量約66kd ，加入iptg誘導8h后，蛋白表達幾乎達到最高水平。 -
Expression vectors of whole il - 6r gene and its carboxyl terminal partial gene sequence were constructed by pcr and gene recombination technique, respectively
6r基因全長和矮基端部分編碼序列的表達載體，並導入大腸桿菌dhsq中，通過iptg誘導表達重組融合蛋白。 -
After sequencing the vectors were transformed into e. coli dh5 a and recombinant fusion protein was expressed via induction of iptg. fusion protein coded by carboxyl terminal gene sequence was purified through glutathione agarose column
經sds page分析，在相對分于質量（ mr ）為74000和43000處，各有1條特異的蛋白帶。 -
Identified as encoding protein that bind msx2, mint, human gene termed as sharp ( smrt / hdac1 associated represser protein ), is a large nascent polypeptide of 3576 amino acids and has three n - terminal rna recognition motifs ( rrms ) and four nuclear localization signals
Mint屬於一個新的spen （ splitends ）蛋白家族成員。 spen是果蠅中一個分子量為600kda的巨大核蛋白，參與果蠅發育過程中神經元蟬的決定和神經元突起延伸的調節。 -
Conclusion : in this paper, we show huccr5 n - terminal gene fragment was cloned successfully and its specific antibody was prpared. by these we not only introduced a simple and quick method to get a specific antibody f ( ab ' ) 2of certain functional domain but also a good idea and technique to study other high similar superfamily members
中有效表達ccrsn端膜外第一謬基因片段，並制備出抗hllccrsn端的特異性抗體f （ ah 』 ） 。 。本方法為一簡捷快速的特定功能結構域抗體廠（ ah 』 ） 。
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