terminal tag 中文意思是什麼
terminal tag
解釋
終端標牌-
Soldering - tag terminal strip
釬焊片端子板 -
Both the mature genes of gloshedobin and gussurobin were cloned into the vector pet - 32a ( + ), strain bl21 ( de3 ), to study their expression in prokaryotic cell. the gene was expressed under t7 promoter with a fusion protein partner of thx. tag and a 6x his. tag at its n - terminal. having been induced by iptg for 4 hours, the recombinant enzyme was examined in the cytoplasm by sds - page analysis
將大連蛇島蝮蛇和長白山白眉蝮蛇毒類凝血酶基因分別克隆到大腸桿菌表達載體pet - 32 （ a ） +中，在t7啟動子下表達出融合蛋白，融合部分為硫氧還蛋白，位於類凝血酶基因上游，並在其n端帶6xhistag標簽以利於表達產物的分離純化，經熱激轉化至宿主菌bl21 （ de3 ）中， iptg誘導斗小時后收獲菌體。 -
The former expresses recombinant proteins with a 6xhis tag at n - terminal. the fore mentioned four fragments were all used for expression in this system and the mature protein and the conservative domain were effectively expressed while the expression of the other two was unobvious
前者表達n ?末端加6 his標記的融合蛋白，克隆到的4個基因片段均進行了表達，其中成熟肽和活性區域得到了大量表達，蛋白全長和末端缺失片段表達不明顯。 -
A human lymphotoxin deletion gene fragment which lacking n - terminal 27 amino acid residues of the protein was pcr amplified using a pair of designed primers and cloned into expression vector pet36b ( + ) to construct a fusion protein with cbd tag, resulting a recombinant plasmid pet36b - lt 27. the recombinant plasmid was transformed into host e. coli bl21 ( de3 ) plyss
採用pcr的方法擴增出人淋巴毒素n端缺失27個氨基酸的缺失體基因片段，將此基因片段克隆至表達載體pet36b （ + ）上，與t7lac啟動子控制下的cbdtag構建成表達融合蛋白的重組質粒pe736b - lt 27 。 -
The sequences flanking tn5 in magnetosome deleted mutant nm4 was cloned by anchored pcr, and was used as tag to extend the 5 ' and 3 ' terminal sequences of the target dna fragment by anchored pcr constinuously
以tn5為標簽，用錨定pcr法克隆出突變株nm4的tn5側翼序列。並以此側翼序列為標簽繼續用錨定pcr法向兩邊延伸，克隆出一個5045bp的dna片段。 -
Hegf gene with his - tag at the end, which was derived from pet22 - egf, was in - frame fused to the carboxy - terminal of polyhedrin ( ph ) gene, which included the amino - terminal 116aa coding region. the polyhedrin - egf fusion gene ( named ph - egf ) was then cut out with ecorv and ecori, and was cloned between ecorv and ecori sites of pbacpak. 8 ( the result plasmid was named pbacph - egf and the ph - egf fusing gene was right under the control of ph promoter ). the pbacph - egf structure was verified with restriction enzymes digestion and pcr
從pet22 - egf質粒中分離出末端帶his - tag的egf基因，對位融合於多角體蛋白n端116個氨基酸基因序列的下游（命名為ph - egf ） ，並在兩段基因間設計了凝血酶xa蛋白酶切位點，經過酶切、測序等鑒定正確后，克隆至pbacpak8中，使ph - egf融合基因置於多角體蛋白（ polyhedrin ， ph ）基因啟動子控制之下，構建成重組轉移載體pbacph - egf 。 -
Purification was conducted with ni - nta resin as there ' s a 6 x his tag at the n - terminal of the recombinant protein. after stepwise washing and elution at purification procedure, sublimated protein was obtained with the full - length ns5 accounting for more than 90 % and the aborted translated protein about 6. 5 %
用ni一nta樹脂親和純化表達產物，經過梯度漂洗和洗脫，獲得純度90 %以上的全長表達產物，另有6 . 5 %的非全長翻譯產物（約70kda ） 。
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