terminal vector 中文意思是什麼
terminal vector
解釋
終末向量-
The hwtx - i gene was chemically synthesized according to its known cdna sequence, the gene was inserted into vector ppic9k which contained aoxj promotor and the sequence of a secreting signal peptide - a - factor, the cloning ppic9k / hwtx - i was constructed and confirmed by two - step pcr and dna sequence analysis, then it was transformed into host strain gs115, a his + muts cell line was screened and multicopy transformants were screened by various g418 concentrations, the multicopy transformant was named gh1. gh1 was cultivated in flasks. after 6 days of induction by 0. 5 % methanol, the supernatant was checked by 16. 5 % tricine - sds page, which showed there was a band in the position of 3. 5 - 6. 1kd, then it was isolated and desalted by ultrofiltration followed by ion exchange of cm column, after reverse phase hplc of ci8 and vacuum drying, the purified rhwtx - 1 was obtained which was proved to be correct recombinant hwtx - i by tricine sds - page, maldi - tof mass spectrometry, amino acid composition analysis, the n - terminal amino acid sequence and its biological activity, the final field of the purified rhwtx - i was about 80mg / l, accounting for 23. 6 % of it total secretory proteins
將帶有hwtx -基因的ppic9k經blgii線性化后，轉化酵母宿主菌gs115原生質體后經篩選陽性克隆並經表型鑒定為his ~ + mut ~ s酵母菌，進一步用遺傳毒素g418篩選多拷貝的轉化菌株，命名為gh1 ；將gh1甲醇酵母菌用0 . 5的甲醇誘導表達，發酵上清經90飽和度的（ nh _ 4 ） _ 2so _ 4沉澱， yw - 3 （ mwc03000 ）的超濾膜超濾，再經cm陽離子交換， c _ （ 18 ）反相hplc純化得到分子量為4kd左右的組分，其中4289 . 05的組分經質譜鑒定，氨基酸組成分析和序列測定為正確的表達產物，生物學活性表明其活性為天然毒素活性70 % ，表達量為80mg / l 。 -
Both the mature genes of gloshedobin and gussurobin were cloned into the vector pet - 32a ( + ), strain bl21 ( de3 ), to study their expression in prokaryotic cell. the gene was expressed under t7 promoter with a fusion protein partner of thx. tag and a 6x his. tag at its n - terminal. having been induced by iptg for 4 hours, the recombinant enzyme was examined in the cytoplasm by sds - page analysis
將大連蛇島蝮蛇和長白山白眉蝮蛇毒類凝血酶基因分別克隆到大腸桿菌表達載體pet - 32 （ a ） +中，在t7啟動子下表達出融合蛋白，融合部分為硫氧還蛋白，位於類凝血酶基因上游，並在其n端帶6xhistag標簽以利於表達產物的分離純化，經熱激轉化至宿主菌bl21 （ de3 ）中， iptg誘導斗小時后收獲菌體。 -
An expression vector carrying a fragment encoding the amino - terminal part of an fr - 008 type i pks module, containing a keto - synthase ( ks ) and part of an acetyl - transferase ( at ) domain was constructed for trial expression of the extremely high g + c content ( 76 % ) pks gene in plant
為探索在植物中表達極高g + c含量的pks基因的可能性，構建了攜帶有編碼fr - 008型pks模塊氨基端部分的基因的表達質粒，包括一個酮基合酶（ ks ）和部分酰基轉移酶（ at ）活性結構域。 -
Firstly the thesis aim at the principle design of the remote hand - writing data sampling system according to the practice demand and establish the software and hardware project to puts it into effect. vector dot matrix was used by rle in the data sampling terminal and ppp was adopted in data transferring
作者主要完成了以下幾方面的工作：第一，根據企業實際需要提出了手寫數據採集系統的原理方案，制定了相應的軟硬體詳細實施方案。第二，設計並實現了數據採集終端的控制軟體和硬體電路，對數據採集終端硬體電路進行了調試，實現了數據採集終端的各項功能。 -
A human lymphotoxin deletion gene fragment which lacking n - terminal 27 amino acid residues of the protein was pcr amplified using a pair of designed primers and cloned into expression vector pet36b ( + ) to construct a fusion protein with cbd tag, resulting a recombinant plasmid pet36b - lt 27. the recombinant plasmid was transformed into host e. coli bl21 ( de3 ) plyss
採用pcr的方法擴增出人淋巴毒素n端缺失27個氨基酸的缺失體基因片段，將此基因片段克隆至表達載體pet36b （ + ）上，與t7lac啟動子控制下的cbdtag構建成表達融合蛋白的重組質粒pe736b - lt 27 。 -
Its function is that the host computer receives alarm and orientation data from mt or sends commands to mt in virtue of modem. sccs makes different kinds of alarm sound according to receiving different kinds of alarm signal, reminding workers of dealing with corresponding alarm in time, and it transmits orientation data to vector map real - timely displaying currently running circumstances of terminal
功能是計算機藉助modem接收來自終端的報警和定位數據或發送命令數據，根據不同的報警類型發出不同的報警聲音，提醒工作人員及時處理警情，同時將定位數據實時傳輸給矢量化地圖顯示終端當前運行情況。 -
Major difference of hn proteins lies in no. 18 - no. 75 amino acid residues on n - terminal which includes three active sites of hn protein. the influence on biological action, caused by the difference of hn protein, is needed to study further. hn gene has only four glycosylation sites, which is 1 or 2 less on amount than that of other strains. so, this difference can be take on as a natural mark of ndv b95 strain furthermore, the fragment encoding hn gene was excised from the positive clone pgem - hn with sail and saci enzyme, and purified by agrose gel fraction method. then the fragment was subcloned into the pet - 28c expressing vector digested by sail and saci restriction enzyme
作者將正確的重組克隆質粒pgem - hn用saii 、 saci雙酶切，電泳回收目的片段，將其亞克隆到經saii 、 saci雙酶切處理的pet - 28c中，轉化大腸桿菌tgi感受態細胞，得到的轉化子經pcr鑒定和酶切分析，篩選出符合閱讀框的重組子，構建成重組表達質粒pet - hn ，並在大腸桿菌bl _ （ 21 ） （ de _ 3 ）宿主菌中成功地表達了含目的蛋白的融合蛋白，融合蛋白的分子量約66kd ，加入iptg誘導8h后，蛋白表達幾乎達到最高水平。 -
Optimal design of system efficiency parameters about terminal - sensitive projectiles based on support vector machine and genetic algorithm
基於支持向量機和遺傳演算法的末敏彈系統效能參數優化設計 -
In order to get small molecular g - protein rab3a, which serves to further investigate the interaction between rab3a and other proteins, we amplified the full coding region of rab3a cdna by polymerase chain reaction, using human placenta total cdna as template. the pcr products were recovered from gel electrophoresis and cloned into bamhi xhoi site of vector pyestrp2. the result of sequencing indicated that rab3a insertion fragment included its initiation and termination codons in 5 - and 3 - terminal, respectively
為了獲取全長的小分子g -蛋白rab3a ，以用於研究rab3a與其他蛋白相互作用關系，本實驗以人胎盤總cdna為模板， pcr擴增到人rab3a cdna全編碼區。產物回收后克隆于質粒pyestrp2的bamhi xhoi位點，測序結果表明，本實驗獲得的rab3a cdna包含了起始和終止密碼子。
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