thawing room解釋解凍室, 解凍間
Your lighter boxes of family papers went up - stairs into a barmecide room, that always had a great dining - table in it and never had a dinner, and where, even in the year one thousand seven hundred and eighty, the first letters written to you by your old love, or by your little children, were but newly released from the horror of being ogled through the windows, by the heads exposed on temple bar with an insensate brutality and ferocity worthy of abyssinia or ashantee
The results indicated that the synthetic mviia was reduced and oxidized in a buffer containing acetamide / guanidine hydrochloride by air oxidization in the room temperature and obtained a relatively well oxidization result, the synthetic mviia showed 100 % of physiological activity of native mviia, but obtained 1 % product of synthetic crude peptide
A small breakfast-room adjoined the drawing-room.
Unpacking area ( exempt if the operations not including unpacking ) unpacking area shall be adjacent to thawing room, cutting and packing room, and freezing room, but separated from cutting and packing room
Western blot analysis we treated 200 fertilized eggs in a series of steps including lysing them in l0ul of lysis buffer ( 50mm tris - hcl ph 7. 5, 250mm nacl, 5mm edta, imm dtt, 0. 1 % triton, 50mm sodium orthovanadate, looug / mg pmsf and tpck, 50ug / ml tlck, 1 ug / ml leupeptin pepstatin and aprotinin ), frozing them below - 70, and then thawing them at room temperature for 10 min
2 . westem印跡將處理組及對照組小鼠一細胞g2期受精卵各200個取出，轉移到ep - pendoff管中， 5000甲m離心4分鐘，棄去上清，加入ro川粉碎緩沖液，充分振蕩混勻，在液氮中經過2一3次的凍融循環，迫使卵細胞裂解，加人等量的樣品緩沖液沸水中煮5分鐘，用於westem印跡分析。