tissue antigen 中文意思是什麼
tissue antigen
解釋
組織抗原-
The western blot analysis show that the antiserum induced by the new antigen plus hemocyanin could bind with the 22kd and 55kd proteins, which were existed in the testis tissue protein of mouse, rat and human. 2 ) the purified peptide emulsified in an equal volume of freund ' s adjuvant and immunized the female balb / c mice with 8 - 10 weeks old. the antiserums and the washings of vaginal membrane were detected by elisa, and shown the highest level of the specific antibody igg was 1 : 6000, while the iga was 1 : 300
二、 p3多肽與弗氏佐劑混懸后免疫近交系balb c小鼠后，在血清和陰道粘膜沖洗液中可檢測出特異性lgg 、 lga ，最高效價分別達到1 ： 6000和1 ： 300 ；免疫后的小鼠脾臟淋巴細胞增殖率升高，淋巴細胞培養上清液中分泌的il 4和inf y也升高，且以il 4更明顯。 -
The expression conditions of e2 gene in p. pastoris were optimized, the results indicated that the peak obtained after 72 hours ; pattern of inhibition / induction could improve expression level ; the best ph value were between 7. 5 and 8. 0 and the optimized methanol - induced concentration was 2 % - 3 % the e2 genes of the prevalent strain ( guangxi yulin strain ) and c strain derived from rabbit spleen tissue were amplified and cloned into e. coli the expression vector pproex - htb respectively, the recombinant plasmids pproex - gxyl and pproex - c were obtained and then were transformed into the dh5a e. coli competent bacteria respectively, the recombinant bacteria could express the major antigen region of e2 gene, the expression yields amount to 35 % and 38 % repectively
豬瘟病毒ez基因的原核表達： pcr擴增出當前豬瘟流行野毒株，中國豬瘟兔化弱毒（ c株）兔脾組織毒ez基因的主要抗原區，將其克隆到原核大腸桿菌表達載體pproex htb中誘導表達，經sds page檢測表明，重組質粒能表達ez基因主要區蛋白， westernblot檢測表明，誘導表達蛋白與豬瘟陽性血清發生特異性反應，表達量為35和38 ，可用於基因工程診斷抗原。 -
The high titer specific ndrg2 antibody is indispensable to reseach deeply the functions or the tissue and subcellular distribution features of ndrg2, ha order to prepare ndrg2 antibody, the whole ndrgl sequence was cloned into prset - a vector and two truncated sequences of ndrg2 were cloned into pgex - 4t - l vector. after induced by iptg, the fusion proteins were expressed in e. coli ; rabbits immunized with the whole length ndrg2 protein were reinforced with two shortened fragments of ndrg2 ; after immunization, rabbits produced high titer antiserum against ndrg2. then antisemm was absorbed using ndrg2 antigen immobilized on nc filters, the purified product of antiserum shows high special to ndrg2 protein, and the separated inclusion body of 6his - ndrg2 will be useful for the further reseach
為制備高效價的ndrgz抗體，分別構建了prset a雌、 pgex4t d唾倉和pgex4tl七三種原核重組表達質粒，並在大腸桿菌中誘導表達出相應的融合蛋白；用全長gstjqdrgz蛋白免疫兔，然後用gst ndrgz人和gstjqdrgze片段加強免疫，經免疫得到了較高效價的兔抗人ndrz多克隆抗血清，利用固定於硝酸纖維素膜上的ndrgz抗原親和吸附純化抗血清，提高了ndrgz抗體的特異性；並對包涵體形式表達的6his ndrgz進行初步的分離純化。 -
There were little difference in the amino acid composition of antigen domain of a, b and c between c strain from rabbit spleen tissue and virulent strains above. this indicated that the prevalence of csf and immunal failure may be not caused by the antigen variation of c stain vaccine virus. the e2 genes of 51 csfv prevalent virulent strains isolated from 12 provinces in china were amplified and sequencd
對c -株兔脾毒與c -株細胞毒、經典強毒e2上的a 、 b 、 c三個中和性抗原區的氨基酸組成進行了比較，其結果為： c -株兔脾毒與c -株疫苗細胞毒的差異很小甚至沒有差異。
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