tmv 中文意思是什麼

tmv 解釋
TMV ＝tobacco mosaic virus 煙草花葉病毒。

  1. Degenerate oligonucleotides to highly conserved regions of cucumis melo 1 - aminocyclopropane - 1 - carboxylic acid ( acc ) oxidase gene were used to prime the amplification of fragment of 128bp by ploymerase chain reaction ( pcr ) in samples of genomic dna from fruit of cucumis melo l. cv hetao flesh, which was cloned into plasmid vector pmd - 18 - t. the clon of antisense orientation were selected, and it was inserted downstream of camv35s promoter and enhancer " " of tmv into the plant expression vector pbinyxw, antisence expression vector pbinya was constructed. at the base that pollination and fertilization of cucumis melo l. cv hetao was studied, using pollen tube pathway transformate cucumis melo l. cv hetao, 76 fruit had been obtained, moreover, hardness and content of sugar were analysed

    本實驗以河套蜜瓜果肉基因組dna為模板，用甜瓜acc氧化酶基因特異寡核苷酸鏈為引物進行pcr擴增，得到128bp的擴增產物。將得到的擴增產物克隆到質粒載體pmd - 18 - t上，篩選反向克隆，然後將其反向構建到植物表達載體pbinyxw的camv35s啟動子和tmv增強子「 」的下游，構建成反義表達載體pbinya 。並在對河套蜜瓜授粉受精生物學研究的基礎上，通過花粉管通道法轉化河套蜜瓜，共獲76顆瓜，並進行了硬度和含糖量的分析。
  2. Studies on virus resistance of transgenic tobacco with tmv replicase gene

    轉煙草花葉病毒復制酶基因煙草的病毒抗性研究
  3. It showed excellent anti - tmv activity. and the n - terminal 19 amino acid residues are " dinfslagadgqtyn tfia " ( accession number p83206 in swiss - prot )

    測得其n -端19個氨基酸序列： dinfslagadgqtyntfia ，與其它植物rip的同源性為10 73 ，與葫蘆科rip的同源性為37 73 。
  4. The resuh twed that mb designed in consisten the the complndny sequence of a sechon of the conservative regions of tmv - rna was suitable for the direet assay of tmv - rna. another contrl experiined had been pefformed to testify that our resuit was re1iable or not

    Of 2inm之間有線性響應，檢測下限達8pm ， t ： ；與共價交聯的固定方法相比，此傳感器具有較長的壽命和較好的穩定性。
  5. The native expressed product from e. coli bl21 ( de3 ) strain, however, showed weak activity against tmv. gp609 and zwemu were inserted into pemu - mcs - n, an expression vector for monocotyledon. and rhxjb was inserted into pkylx71 : 35s2

    將dna一8001連接到pet一sa表達載體上，在大腸桿菌blzi （ de3 ）菌株中實現天然表達，表達產物對tmv的抑制效果很差，其原因可能是c一末端延伸序列的存在抑制了抗tmv活性的發揮。
  6. Sds - page results showed that there was a clear target protein band in mut + recombinant supernatant after 48 hours of culturing, while a faint band only in muts recombinant after 72 hours. western - blotting result showed that there was no remarkable difference of yield between mut + and muts recombinants after 6 days induced. anti - virus activity tests revealed that culture supernatants of mut + and muts recombinants could inhibit tmv infection with high efficiency in the same concentration and there was no significant difference between them

    結果表明，誘導培養48小時后， mut ~ +重組菌株表達產物在sds - page膠上顯現出清晰的目的蛋白帶，而mut ~ s重組菌株培養72小時才能顯示微弱的目的帶； western - blotting雜交信號強度表明，同樣培養6天的mut ~ +和mut ~ s重組菌株表達產物在表達量上沒有明顯差別。
  7. Both lines showed resistance to tomv - 0, tomv - 2, tomv - 1, tmv - u, tmv - cg and susceptible to tomv - 2a. this result indicated that tm - 22 was functionally expression in receptor cell with distinct genetic background. virus specificity and gene express can function in distinct genus within a family

    為了了解tm - 2 ~ 2基因的特性、功能及抗病機制，研究工作從三個方面進行，首先將tm - 2 ~ 2基因轉化同科異屬的煙草sr1 ，以確定tm - 2 ~ 2基因在模式植物的表達的可能性和功能的完整性。
分享友人
分享到 Line

分享到臉書