transfer dna 中文意思是什麼
transfer dna
解釋
轉化-
Because of the special biological structure of bryology, it was very difficult to transfer foreign gene into the protonema or gametophyte by agrobacterium - mediated transformation. protoplasts as acceptor, using direct dna transfer methods such as microprojectile bombardment and peg - mediated transformation is becoming a good way
由於蘚類植物特殊的生物學結構用農桿菌侵染其原絲體或者莖葉體很難實現轉化，以原生質體作受體是蘚類植物轉化的常用途徑。 -
The recombinant plasmid puge dna and transfer vector pfastbacl dna were treated again in the same enzyme, were linked by means of t4 dna ligase and transformed into e. coli jm109 permissive cells, yielding recombinant transfer vector plasmid pfastbac - ge dna and were transformed into dhlobac containing vector bacmid
將重組質粒pugedna與轉移載體pfastbacldna用bamhi和ecori雙酶切處理， t _ 4dna連接酶連接，用連接產物轉化大腸桿菌jm109感受態細胞，得到重組轉移載體質粒pfastbac - gedna 。 -
Recent studies show that dna transfer and recombination technology provides great potential for genetic improvement of zoysiagrasses ; molecular linkage maps were constructed from zoysia japonica and its hybrids with zoysia matrella ; the researches on gene cloning and gene resource are in progress
最近的研究表明，基因轉移與重組技術在結縷草遺傳改良中具有巨大應用潛力；結縷草遺傳連鎖圖譜構建取得重要進展；基因克隆和基因資源研究正在展開。 -
The recombinant transfer vector pbacpak - hbmp was constructed by insertion of the hbmp coding sequences into the multiple cloning site of transfer vector pbacpak. 8. bmn cell line was co - transfected with pbacpak - hbmp plasmid and linearized baculovirus bacpak6 dna by dosper agent
將克隆到的hbmp基因通過適當的酶切插入到轉移載體質粒pbac - pak8的多克隆位點中，獲得重組轉移載體質粒pbacpak - hbmp 。 -
The electrochemical study showed that the interaction mode is mainly intercalative binding in ph 7. 4 phosphate buffer solution. the uv - vis spectroscopic study further demonstrated the above results. through the electrochemical parameters such as charge transfer coefficient and standard electron transfer rate constant ks in the absence and presence dna, it was found that the reaction of aloe - emodin with dna forms an electrochemical active supramolecular complex
本文對姜黃素的研究結果表明，在0 . 1m磷酸鹽緩沖液（ ph3 . 0 ）中，姜黃素于玻碳電極上存在可逆的單電子轉移過程，據此，本文建立了以差示脈沖伏安掃描法檢測姜黃素含量的新方法。 -
Different amount of copies in different tissues attribute to the different density of positive signals. the result of the experiment suggested that the transgenic animals can be produced by spermatozoa - mediated gene transfer after the entrapment of liposome. and because the exogenous dna occurs losing the segments. partly integration, or existin g outside of genome dna, the rate of chimerism is relatively high
結果表明： （ 1 ）脂質體包裹外源基因轉染精子的方法，可將外源基因導入受精卵中，能夠獲得轉基因動物，並得到了較高的轉基因陽性率； （ 2 ）精子攜帶的外源dna的整合過程是隨機的，在受精過程和胚胎早期分化過程中可能發生了片段丟失、不完全整合或游離于基因組存在而產生嵌合體。 -
The angiostatin baculovirus transfer vector was co - transfected with viral dna into sf9 cells according to the manufacturers protocol. to purify the recombinant virus, we used the plaque assay to screen the pure recombinant plaque and amplify it to generate p - 1 stock
構建重組病毒：用已經構建好的angiostatin桿狀病毒轉移載體pbluebachiszb和病毒dna共同轉染sfg細胞，通過蝕斑實驗篩選出純的重組斑點並擴增產生p二病毒貯存液。 -
With precious human eggs too few and far between, cibelli spent his time between deliveries practicing the transfer of nuclear dna from a half - dozen or so body cell donors into cow eggs just to perfect his techni * ue
由於人類卵子嬌貴而稀少，奇貝利通過將幾個人體細胞捐贈者的細胞核dna注入牛的卵子中來磨練技巧。 -
With precious human eggs too few and far between, cibelli spent his time between deliveries practicing the transfer of nuclear dna from a half - dozen or so body cell donors into cow eggs just to perfect his technique
由於人類卵子嬌貴而稀少，奇貝利通過將幾個人體細胞捐贈者的細胞核dna注入牛的卵子中來磨練技巧。 -
Coding mechanics of a hh neuron, electron transfer in a dna molecule, protein analysis, heartbeatgait time series
序列分析、神經元等細胞內信息編碼機制、 dna分子內電子傳輸、蛋白質分析、心律步伐序列分析等的應用。 -
In order to get the soluble recombinant eo protein and inspect the protein expression status convinently, the egfp and eo gene were ligated into baculovirus transfer vector. with the co - transfecting sf9 cells of baculovirus recombinant transfer vector and linearized viral dna, and plaque purification in the posttransfection procedure, the pure recombinant baculovirus were harvested, which infected the sf9 cells for amplifying to generate a p - l stock. in the meantime, the fluorescence microscopy detection indicated expressed egfp protein to confirm the heterogenous protein expression of recombinant baculovirus. the pi - stock from a pure plaque was used to generate a high liter p - 2 stock, which was determined in liter as 1. 14 107pfu / ml by performing a plaque assay. when a volume of p - 2 stock infected the sf9 cells with moi 5 - 10 for expression, the strong fluorescence was obeserved on the day 3 of postinfection
此外，為了得到可溶性重組eo蛋白並便於觀察重組蛋白的表達情況，我們將egfp基因與eo基因相連插入昆蟲桿狀病毒轉移載體中，與線性桿狀病毒dna共轉染sf9細胞后通過噬斑純化得到純的重組桿狀病毒，將其感染sf9細胞制備p1種子液，同時用熒光顯微鏡觀察綠色熒光蛋白的表達情況剔除表達效果差的重組桿狀病毒。再用p1種子液感染sf9細胞制備高效價的p2種子液。通過病毒液的梯度稀釋和噬斑測定，確定p2種子液的病毒滴度達1 . 14 10 ~ 7pfu ml 。 -
In this study, a 1. 7kb kpni fragment and a lacz gene expression cassette carrying the e. coli lacz gene under the control of sv40 promoter were inserted into the transfer vector pbdtk - uni ( a 277bp acci - accl fragment in the tk gene was deleted ). the new transfer vector was called puni - lacz. the transfer vector puni - lacz and purified genomic dna of strain bartha - k61 were used to cotransfect vero cells using lipofectin transfection procedure
本研究以呈ge ~ -表型的經典弱毒疫苗bartha - k61株為親本株，在通用prv轉移載體pbdtk - uni的基礎上，在其多克隆位點中插入由sv40早期啟動子控制下的lacz基因表達盒，同時將下游同源臂增加了一個1 . 7kb的kpni片段，使上下游同源臂的長度都超過了1kb ，構建了一個新的轉移載體puni - lacz 。 -
The new genetic laws observed recently in the ion - beam - induced wheat, and the advance in wheat breedings through ion - beam - mediated transfer of null dna molecule, were also presented thus pointing out the critically unsolved questions and perspectives of ion beam technology in the field of wheat breedings
還介紹了最近發現的離子束誘變小麥遺傳新規律和離子束輔助外源裸露大分子dna轉移在小麥育種中的新進展，提出了離子束生物技術在育種領域急待解決的問題和應用前景。 -
In this thesis, the energy transfer system of acridine orange ( ao ) - rhodamine b ( rb ) dimer was built for the determination of dna as fluorescence probe
本論文以吖啶橙?羅丹明b二聚體能量轉移體系作為熒光探針，建立了測定dna的新方法。 -
Then bm cell line was co - transfected with parental bm - npv dna and the transfer plasmid pvl - nk, the recombinant virus was then obtained subsequently with routine procedure. nattokinase was expressed in silkworm larva by injection with the recombinant virus
將nk基因克隆至轉移載體pvl - 1393中後用常規方法獲得重組病毒，感染家蠶后納豆激酶獲得正確表達。 -
Arginine rich human histone h3 is a kind of basic nucleosome protein. in the medium of physiological condition, by electrostatic interaction, histone h3 which arginine impart to it a positive charge binds to dna which phosphate groups impart to it a negative charge. in this paper, in order to establish a new technology of transfection, the functional domains la and ii gene segments of pea were lined with histone h3 gene segment, then a recombinant fusion protein was constructed which serves as c arrier for transfer of dna via receptor - mediated endocytosis
本實驗在已成功獲得的pea功能區a ，功能區基因片段與人組蛋白h3基因片段的克隆菌株的基礎上，構建原核表達載體，將克隆質粒pmd - 18t - pea經nco ， mlu雙酶切，回收酶切產物；將克隆質粒pmd - 18t - h3經mlu ， xho雙酶切，回收酶切產物。 -
Dna sequence analysis showed that the cloned nk gene was highly homologous to the sequence reported by nakamura. the deduced amino acid sequence also had the conserved sequences ( serine 221, histidine 64, and aspartic acid 32 ) which was essential for the catalytic center of serine proteases. the nk gene was then cloned into the transfer vector pvl1393
本實驗以b . subtilis （ natto ）基因組dna為模板擴增了nk基因，測序結果顯示與nakamura報道的納豆激酶基因高度同源，其氨基酸序列也含有絲氨酸蛋白酶的活性保守中心（ serine221 ， histidine64 ， asparticacid32 ） 。 -
Sequences flanking tn5 - 1063a can be recovered from the genome of mutant by excision, self - ligation and transfer to e. coli. the total dna of mutant was excised with ecori, which cut the genome frequently but not cut the transposon. after sequencing the self - ligated transpon, dna fragment flanking tn5 was obtained. the result showed 042bm - x1 contains a tn5 insertion in the gene smc00190, which function was unknown and was demonstrated to be related to salt tolerance by this study, and the gene was named as rst - 0x1
通過ecori酶切突變株基因組，得到完整的tn5 （含有在大腸桿菌中起始復制的oriv ）及其側翼的序列片段，該片段自連后轉化大腸桿菌，以tn5兩端已知的序列設計引物進行測序。 blast的分析測序結果表明， 042bm - x1和042bm - x2中tn5分別定位在苜蓿中華根瘤菌1021染色體上smc02682和smc00419基因內部，本實驗證明它們和042bm耐鹽相關，命名為rst - 0x1和rst - 0x2基因。 -
Fusion gene by pcr was inserted into bombyx mori baculovirus transfer vector pbacpak. 8 and contransfected with lineared dna of bm - bacpak6 virus into bmn cells. the homologous recombination occurred inside the cells, and the recombinant virus bacpak - 6aa - hgm - csf was expressed, as identified by pcr and southern hybridization. the bmn cells and the fifth instars were infected by the recombinant virus bacpak - 6aa - hgm - csf
本研究首先通過pcr將家蠶桿狀病毒多角體蛋白起始密碼子后的18個堿基引入到hgm - csf基因的5 』端之前，然後將融合基因重組與家蠶桿狀病毒轉移載體pbacpak8中，獲得重組轉移載體pbacpak8 - 6aa - hgm - csf ，並與線性化bm - bacpak6dna共轉染家蠶細胞株，獲得重組病毒bacpak - 6aa - hgm - csf 。
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