transformed cell 中文意思是什麼

transformed cell 解釋
變異細胞
  • transformed : 使. . . 變形
  • cell : n 1 小室,單室;隔間,艙;〈詩〉茅舍;(單個的)蜂窩,蜂房。2 〈詩〉墓穴,墓。3 (大修道院附屬的...
  1. The e. coli strain jm109 was transformed with resultant plasmid pgex - 4t - 1 / 6 - 4. 4. the transformation was induced with iptg, then the total protein from cell extract was analyzed by electrophoresis on a 8 % sds - page in order to validate the gst fusion protein, and the fusion protein is about 90kd

    4 .用工ptg誘導含pgex一4t一1 / 6一4的轉化菌,提取初提物中的總蛋白,進行sds一聚丙烯酞胺凝膠電泳,檢測表達的融合蛋白大小越為gokd 。
  2. Metformin, an antihyperglycemic agent used in type 2 diabetes, was also inestigated because it inhibits ptp opening in transformed cell lines

    二甲雙胍是用於治療2型糖尿病的降糖藥,能夠抑制轉化細胞系ptp的開放,所以我們對其進行調查
  3. Somatic hybrid cells were obtained between ethionine resistant cell line of astragalus adsurgens pall, and agrobacterium / " / z / zogerae. s ' - transformed cell line of medicago saliva l. using protoplast fusion by peg method

    通過peg誘導原生質體融合和雜種細胞的篩選培養,首次得到沙打旺( + )紫花苜蓿的屬間體細胞雜種。
  4. After the recombinant plasmid pcdna3. 1 / ts87 was identified by digestion of hindlll and bamh i, it transformed into cos7 by lipofectamine. expression product was identified by immunohistochemical method, sds - page and western - blot. the immunocytochemistry result has shown that specific brown - staining grains were found in the cytoplasm of cells transformed by recombinant plasmid versus not seen in cells transformed by pcdna3. 1 or normal cells ; the sds - page result has revealed that a band about 3 8kb was found in cell lysis transformed by recombinant plasmid versus not in cells transformed by pcdnas. l or normal cells ; the western - blot result has showed that only the band about 38kd was recognized by sera from rabbit infected by t. s artificially and sera from rabbit immunized with soluble antigen of t. s and with protein expressed by ts87 gene and by a monoclonal antibody of t. s

    通過細胞的免疫組化,細胞裂解物的sds - page電泳, westem - blot分析檢測目的基因的表達情況。免疫組化結果顯示:重組質粒轉染的細胞質中有棕褐色顆粒,而空載體轉染細胞及正常細胞無此現象;細胞裂解物sds - page電泳結果顯示:只有重組質粒轉染的細胞在約38kd處有明顯的蛋白帶,這與理論計算的ts87基因表達蛋白的分子量為38kd基本一致; western - blot分析結果顯示:約38kd的蛋白帶能夠分別被旋毛蟲感染兔血清,成蟲蟲體可溶性抗原免疫兔血清, ts87基因原核表達蛋白免疫兔血清( ts87血清)以及一株具保護性的旋毛蟲單抗特異識別。
  5. In this paper, rebar corrosion state was judged with three electrochemical nondestructive measuring technologies, i. e. half - cell potential, a. c. impedance and time potential. when the rebar was transformed from passivation to depassivation, it can obtain the chloride ions corrosion critical content through taking and analyzing chloride ions content around the rebar

    本文利用半電池電位法、交流阻抗法和時間電位法三種電化學無損檢測技術判斷評估試件在試驗過程中鋼筋腐蝕狀況,當鋼筋由鈍化狀態轉為活化狀態時,取樣分析鋼筋周圍氯離子含量,得到了不同技術條件混凝土的氯離子臨界濃度。
  6. Then the linked products were transformed into the high competent cell of e. coli dh5a. based on - complementation of the detective - galactosidase, positive recombinant clone were screened from x - gal plate

    從引物和基因序列的比對分析結果看, zhyf006序列與上下游引物的配對比例分別為s4
  7. The hwtx - i gene was chemically synthesized according to its known cdna sequence, the gene was inserted into vector ppic9k which contained aoxj promotor and the sequence of a secreting signal peptide - a - factor, the cloning ppic9k / hwtx - i was constructed and confirmed by two - step pcr and dna sequence analysis, then it was transformed into host strain gs115, a his + muts cell line was screened and multicopy transformants were screened by various g418 concentrations, the multicopy transformant was named gh1. gh1 was cultivated in flasks. after 6 days of induction by 0. 5 % methanol, the supernatant was checked by 16. 5 % tricine - sds page, which showed there was a band in the position of 3. 5 - 6. 1kd, then it was isolated and desalted by ultrofiltration followed by ion exchange of cm column, after reverse phase hplc of ci8 and vacuum drying, the purified rhwtx - 1 was obtained which was proved to be correct recombinant hwtx - i by tricine sds - page, maldi - tof mass spectrometry, amino acid composition analysis, the n - terminal amino acid sequence and its biological activity, the final field of the purified rhwtx - i was about 80mg / l, accounting for 23. 6 % of it total secretory proteins

    將帶有hwtx -基因的ppic9k經blgii線性化后,轉化酵母宿主菌gs115原生質體后經篩選陽性克隆並經表型鑒定為his ~ + mut ~ s酵母菌,進一步用遺傳毒素g418篩選多拷貝的轉化菌株,命名為gh1 ;將gh1甲醇酵母菌用0 . 5的甲醇誘導表達,發酵上清經90飽和度的( nh _ 4 ) _ 2so _ 4沉澱, yw - 3 ( mwc03000 )的超濾膜超濾,再經cm陽離子交換, c _ ( 18 )反相hplc純化得到分子量為4kd左右的組分,其中4289 . 05的組分經質譜鑒定,氨基酸組成分析和序列測定為正確的表達產物,生物學活性表明其活性為天然毒素活性70 % ,表達量為80mg / l 。
  8. The full length cdnas of 17 - hsd1 and 17 - hsd3, 17 - hsd8 were obtained by library screening and race, respectively. expression patterns ( tissue distribution ) of three types 17 - hsds were checked by rt - pcr and northern blot. the recombinant constructs of 17 - hsdl / pet blue2 and 17 - hsd8 / pcdna 3. 1 were made and subsequently transformed into the corresponding host expression cell of ( de3 ) placi and mammalian hek 293 cell

    首先從genebank下載在其他脊椎動物中已被克隆17 - hsd1 , 17 - hsd3的氨基酸和核甘酸序列,並在序列保守區域設計簡並引物,然後分別以羅非魚卵巢和精巢cdna為模板進行rt - pcr擴增得到17 - hsd1 , 17 - hsd3的中間片段。
  9. Chromosome numbers were no remarkable difference with the control. analysis of isoenzyme ( peroxidase, cytochrome oxidase, esterase ) and rapd indicated that the regenerated plants from protocalli were different with the control. protoplasts were isolated from agrobacterium rhizogenes a ^ transformed cell line of medicago sativa l.

    染色體檢查,同工酶(過氧化物酶、細胞色素氧化酶、酯酶)和rapd分析表明,來源於抗性系原生質體的再生植株具一定的遺傳穩定性,但與野生型相比亦發生了一些變異。
  10. Then, we transformed those two genes into tomato and tobacco plants via agrobacterium tumefaciens. after verified by antibiotic resistance, reporter gene examination, southern blot detection, and genetic segregation analysis, we obtained 3 and 7 transgenic tobacco plants with one copy of rbcs 3a - gus or rbcs 3c - gus gene, respectively. further, we established two suspension - cultured cell lines using above mentioned two kinds of transgenic tobacco plants

    對得到的再生煙草植株分別進行了報告基因表達水平檢測、 southern - blot鑒定以及t _ 0代轉基因種子遺傳分離規律分析,分別得到了單拷貝的穩定表達番茄rbcs3a - gusa 、 rbcs3c - gusa和35s - gusa基因的轉基因煙草3株、 7株和1株,同時還得到單拷貝的只轉化gusa基因的陰性對照轉基因煙草3株。
  11. After electrophorised on 1 % agarose gel, the pcr production was purified with agarose gel dna extraction kit. the segment was ligated with vector pmd18 - t and then was tranformed into the competent cell of dh5 a. a construction mstnd - pmd18t was generated by inserting the sequence of 254bp into pmd18 - t vector and selecting the sense clones. positive clone was identified by three ways : endonuclease digestion, pcr and sequencing. the result showed that the cloned sequence coincides with the designed sequence. this construction was digested with nco i and xho i and ligated the pet28a ( + ) vector digested with the same enzymes using dna ligation kit. the production of ligation reaction was transformed into the competent cell of bl21 ( de3 ). after 12 - 16 hours of culture, several colnes appeared on the plate. some positive clones were selected to extract their plasmid. these plasmids were digested by nco i and xho i and indentified by pcr. a contraction, mstnd - pet28a was generated. the result showed that the cloned sequence coincides with the designed sequence

    F _ 1長38bp , r _ 1長36bp ,其它片段均40bp長, f _ 1和r _ 1片段兩端分別加上限制性內切酶nco和xho的識別位點序列。用成對單鏈片段進行延伸反應,然後用其他單鏈片段作為引物,進行pcr擴增,用dna快速純化回收試劑盒回收所得254bppcr產物,與pmd18 - t載體連接、轉化dh _ 5 。受體菌感受態細胞,利用藍白斑遺傳學篩選法篩選陽性克隆,提取其質粒,採用nco和xho雙酶切鑒定,獲得了254bp的片段;用pmd18 - t載體上的特異引物rv - m和m13 - 47進行pcr鑒定,獲得300bp的片段。
  12. Methods the 54th generation of transformed human embryonic tendon cells and artificial composite materials of carbon fibers ( cf ) and polyglycolic ( pga ) were co - cultured in vitro to construct tet. lt was frozen in liquid nitrogen with four kinds of cpa for 2 months. post - thawed quickly and transplanted into hind limbs of nude mice, and repaired the defects of achilles tendon. after 2, 4, 6, 8, 12 weeks, the morphological, histological, ultrastructure, short tandem repeat loci and immunohistochemistry examination were detected, and biomechanical strength of tet were examined. result tendon cell survived and could secret type i collagen after 12 weeks to transplanted into nude mice. in the group of dmso + raffmose + kh2o4, vacuole in mitochondrion degraded i tendon cell ranged in order, abundant collagen fibers were found and linked each other and the biomechanical strength was increased as time elapsed. c onclusion dmso + raffmose + kh2o4 could protect tet in deep low temperature

    組織工程肌腱制備完成後在四種抗凍劑保護下液氮凍存2月;快速復溫后植入裸鼠以修復跟腱缺損, 2 、 4 、 6 、 8 、 12周后取出,觀察形態學、組織學、電鏡和免疫組織化學變化,短串聯重復位點檢測和生物力學變化。結果實驗組組織工程肌腱體內植入12周后仍有肌腱細胞存活並分泌型膠原;隨著時間延長, 10二甲基亞碸( dmso ) +棉子糖( 30mmol l ) + kh _ 2po _ 4 ( 25mmol l )組線粒體空泡減少,肌腱細胞排列整齊,膠原纖維增粗並連接,抗拉強度增高。
  13. Some systems allow the irus to infect cells but do not permit prolonged replication and production of irus, while other systems rely on deriaties of permissie irus isolates for efficient replication in transformed ( mutated ) cell lines

    一些培養體系可使病毒感染細胞而不能促進其大量復制繁殖,還有一些培養體系需要誘導劑使病毒在突變細胞株內充分復制。
  14. Transformed, oncogenic precursors, possessing both defining neural - stem - cell properties and the ability to initiate intracerebral tumours, have been identified in human brain cancers

    人類腦部腫瘤中已經證實,突變的致瘤前體細胞不僅具有神經幹細胞性能並且能致腦部腫瘤發生的潛能。
  15. Panning and enrichment ofmg7 recombinant phage antibody the transformed cells were infected with m13k07 helper phage to produce a phage form of mgy recombinant phage antibody library ; after three rounds panning of the library with the mgrbinding antigen highly expressed gastric cancer cell line kato iii, the mg7 scfv displayed phage dones were selected from the enriched recombinant phages by elisa

    3 mg _ 7重組噬菌體抗體庫的淘篩用m13ko7輔助噬菌體感染轉化菌,以挽救出噬菌體形式的抗體庫;用高表達mg _ 7抗原的胃癌細胞系kato對抗體庫進行三輪淘篩;用elisa篩檢呈現有mg _ 7scfv的噬菌體單克隆。
  16. Rapl belongs to ras superfamily of small gtp - binding proteins, which is considered to control cell growth, differentiation and survival. rapl was regarded as an antagonist of ras because it can revert the phenotype of oncogene ras transformed cells. recent researches show that rapl has its ras - independent functions in a variety of cellular systems besides its effect as an antagonist of ras

    Rap1除具有逆轉原癌基因ras激活突變體所導致的細胞形態改變而被認為是ras的拮抗物外,最近的研究還發現rap1在一系列的細胞系統中有獨立於ras之外的功能,如控制與細胞粘附有關的事件等,因而成為研究的熱點。
  17. Sequence analysis shows that they share 98. 75 % similarity at the dna, and 98. 67 % at the protein level. ns2 gene was cloned into the multiple cloning sites of prokaryotic expression vector pgex - 6p - l. the recombinant plasmid pgex - 6p - ns2 was constructed and transformed to the competent cell bl21 ( de3 ) plyss, positive bacterium strain was induced by iptg

    將ns2基因插入到原核表達性質粒pgex - 6p - 1的ecor 、 bamh多克隆位點之間,將重組原核表達質粒pgex - 6p - ns2轉化到bl21 ( de3 ) plyss感受態細胞中,獲得了表達ns2基因的陽性亞克隆重組子,在含amp的lb液體培養基中培養,經iptg誘導表達,用sds - page分析表達產物。
  18. In this study, pichia pastoris system had been utilized for expression of fmdv 2c3abc gene which aimed for establishing a sensitive and specific molecular dignosis method. first, 2c and 3abc genes were amplified individually from p2 and 3abc postive clones and ligated together using pcr method, then this 2c3abc product was cloned into pgem - t easy vector and transformed e. coli dh5a competent cell. a postive recombinant plasmid which contained whole 2c3abc gene had been confirmed by pcr, enzyme digestion and sequencing. after that, the 2c3abc gene was sub - cloned into ppiczaa expression vector and transformed e. coli dh5 a competent cell and selected by zeocin ? antibiotic. the postive recombinant expression vector was linerized and electro - transformed pichia pastoris smd1168 competent cell. a recombinant pichia pastoris had been obtained by zeocin ? antibiotics selection and induced with 0. 5 % methanol for target protein expression. the expression product was analysised by sds - page and western blotting assay. the result sh owed that 2c3abc gene was expressed successfully in pichia pastoris and the product was a 95ku fusion protein which could be recognized by anti - fmdv serum. the amount of target protein was over 15 % of the total bacteria protein by gel thin layer scanning analysis. this research had supplied materials for establishing a fmd diagnosis method to differentiate infected animals from vaccinated animals

    首先,用p2和3abc陽性克隆通過連接pcr方法獲得目的基因並將其克隆到pgem - teasy載體上,並轉化e colidh5a感受態細胞中,經pcr 、酶切以及測序證明得到了完整的2c3abc基因,並與國內外參考序列進行比較分析。然後,將目的基因亞克隆于ppiczaa表達載體並轉化大腸桿菌dh5a ,以zeocin ~ ( tm )抗性篩選陽性克隆,大量提取重組表達質粒並用pme酶線性化后電轉化入畢赤酵母smd1168感受態細胞,通過zeocin ~ ( tm )抗生素梯度濃度篩選,獲得重組酵母用0 . 5甲醇誘導表達,通過sds - page電泳、 westernblotting分析,結果表明, 2c3abc基因在畢赤酵母中成功表達,其表達產物為一95ku的融合蛋白,並能被口蹄疫病毒陽性血清識別。
  19. A recombinant expression plasmid was first constructed by inserting the vitreoscilla hemoglobin gene ( vgb ) into the plasmid pchi, which contains the chitinase gene cloned from bacillu circulans c - 2. to study the effect of vhb on cell growth and chitinase production, the expression plasmid was transformed into escherichia coli jm109

    為了進一步提高幾丁酶基因在大腸桿菌的表達,將透明顫菌血紅蛋白基因( vgb )插入到幾丁酶基因表達質粒pchi后轉入大腸桿菌jm109中, vgb基因的表達促進了轉化菌的生長和提高了幾丁酶基因的表達,溶氧水平越低,作用越顯著。
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