tris buffer 中文意思是什麼
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By means of discontinuous tris - hcl buffer system and poly aery 1 amide gel electrophoresis ( page ), zymogram of 23 species of 11 genera in 4 subfamilies mirinae, orthotylinae, phylinae and deraeocorinae are gained. by specific primer pcr and sequencing, 57 cyt b gene 433bp sequences are obtained from 34 species respectively belonging to 17 genera in 5 subfamilies mirinae, orthotylinae, phylinae, deraeocorinae, bryocorinae from 64 species of 23 genera which involved in this research, and 2 outgroup species of family anthocoridae as well. 1
首次通過特異引物擴增和基因測序的研究方法從被試的5亞科23個屬的64種盲蝽中獲得了盲蝽亞科、合墊盲蝽亞科、葉盲蝽亞科、赤爪盲蝽亞科和蕨盲蝽亞科bryocorinae5個亞科17個屬的34種盲蝽以及外群花蝽科anthocoridae2種花蝽的cytb基因序列57條。 -
In order to produce monoclonal antibodies, first, several v. dahliae isolates were grown in liquid czapeak medium, after rinsing mycelia and eliminating zoospores, the fungal tissue was homogenized with the pestle in liquid nitrogen and then transferred to test tubes and was centrifuged in tris - hcl buffer
在制備抗原的過程中，首先液體振蕩培養了若干株棉花黃萎病菌，經過沖洗除孢子、液氮研磨，用tris - hcl抽提，再離心制得菌絲蛋白提取液，可作為電泳樣品。 -
At first, 1. 67 u g per well mcab all was coated on three wells of a plate, and then 1. 5 x 1011 phage virion was diluted and added, after incubating with the target, wash away unbound phage by tbst ( 0. 1 % tween - 20 ), the bound phage was eluted with ph 2. 2 tris - gly buffer and amplified, the specially bound phage was enriched by taking through addition binding / amplification cycles. ln the following cycles, the stringency of panning can be increased by raising the concentration of tbst or decreasing that of mcab all, collecting and titering the washing phage of last time and output phage in each round, the selective ratio and the false positive rate of each round were worked out, the gradually increasing of selective ratio and decreasing of positive rate shows that the panning was effective. after 4 rounds of panning, 11 phage clones were selected after competitive - ellsa, the dna samples of 8 positive clones and 1 negative clone were sequenced and all the foreign peptides inserted was also deduced, a clear consensus binding sequence emerged
在本實驗中，利用隨機12肽庫對抗豬瘟病毒（ classicalswinefeverviruscsfv ）糖蛋白me2的單抗a11進行表位篩選，經過四輪篩選以後，隨機挑取11個克隆作競爭- elisa檢測，結果表明，所挑11個克隆中，有9個克隆能對me2蛋白和a11反應產生抑制作用，抑制率最高可達64 ； dna測序以後經過dnastar軟體分析，發現它們的核心序列為anwralsl ，該核心序列與豬瘟病毒e2蛋白的28 - 35位氨基酸ttwkeysh具有同源性；夾心- elisa檢測和western - blotting試驗均證明所挑陽性克隆能被a11所識別；人工合成含核心序列的多肽經間接elisa試驗證實，也能被a11識別。 -
However for the closed stomata, induced by aba, the distribution density of particles in gcv is greatly reduced with the obviously enlarged volume, b ) buffers regulate ph of in vitro gcv sap in the tip of micro - capillaries : certain amount of buffer ( mes - tris ) is filled from the tip of micro - capillaries beforehand, and the ph of gcv sap that is sampled by the same micro - capillary sh ould be regulated by the buffer
觀察結果與本試驗室已發表的氣孔開放過程的實驗結果一致：開放態氣孔保衛細胞液泡所含顆粒狀物的線度極小（平均直徑10nm ） ，且分佈密度極高，而經aba處理的關閉態氣孔保衛細胞液泡所含顆粒狀物的線度很大（平均直徑50nm ） ，分佈密度很小。 -
Western blot analysis we treated 200 fertilized eggs in a series of steps including lysing them in l0ul of lysis buffer ( 50mm tris - hcl ph 7. 5, 250mm nacl, 5mm edta, imm dtt, 0. 1 % triton, 50mm sodium orthovanadate, looug / mg pmsf and tpck, 50ug / ml tlck, 1 ug / ml leupeptin pepstatin and aprotinin ), frozing them below - 70, and then thawing them at room temperature for 10 min
2 . westem印跡將處理組及對照組小鼠一細胞g2期受精卵各200個取出，轉移到ep - pendoff管中， 5000甲m離心4分鐘，棄去上清，加入ro川粉碎緩沖液，充分振蕩混勻，在液氮中經過2一3次的凍融循環，迫使卵細胞裂解，加人等量的樣品緩沖液沸水中煮5分鐘，用於westem印跡分析。 -
We used gradient dilution renaluration ( renaturation buffer : 0. 14mol / l tris - hcl ph8. 0. 10mmol / lgsh, 2mmol / lgssg. 5 % glycerol, 0. 02mol / l l - arg ) and gel filter renaturation by sephacral s200 to refold the inclusion body respectively. to get pure nk4, it was purified by gst affinity gromatochraphy. to detect the angiostatic activity of nk4 in vivo, it was tested using cam
6 ）誘導溫度對pg廠x一4t一l一nk4表達菌株bl21表達目的蛋自的影響分別進行了25 』 c 、 300c 、 37溫度狀態卜，以iptg化學誘導，發現其均可表達，全菌表達量: 37最高，其次為30誘導時。
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