trna 中文意思是什麼

trna 解釋
氨基酰
  1. The complete nucleotide sequence of the mitochondrial genome of f. limnocharis was detailedly compared with those of 5 other amphibians. the nucleotide sequences of 22 trna encoded by 6 amphibians mitochondrial genomes were combined and aligned to the homologous sequences of the 11 veterbrate taxa. using teleosts as outgroup, the phylogenetic analyses results show that mp, nj and ml trees all strongly support the monophyly of living amphibians with respect to other living tetrapods and favor a sister group relationship for caecilians and salamanders

    我們在測定了澤蛙線粒體全基因組序列的基礎上,與已知其它的5種兩棲類進行詳細的比較分析,同時選擇了11種高等脊椎動物的線粒體全基因序列,以硬骨魚類做外群,用22個trna基因合併數據進行系統發生重建分析,結果表明mp 、 nj和ml樹都強力地支持現生兩棲類動物為單系群並且蠑螈類和蚓螈類為姐妹群關系(自引導值分別為92 、 99 、 100 ) 。
  2. A four - gene block, a rwo - trna gene cluster and six single trna genes involved in the rearrangement, the second gene rearrangement type of mitochondrial dna reported for any pancrustacea arthropod. comparisons of mitochondrial gene arrangements of decapod suggested that rearrangement of the four gene - block found in eriocheir is informative for high level phylogenetic study of decapod

    發生重排的基因包括一個4基因塊的重排、 1個2基因的trna基因簇的重排和6個單一trna基因的重排,形成泛甲殼類線粒體dna的另一種基因重排類型。
  3. Like mrna, both trna and rrna are transcripts of chromosomal dna.

    TRNA及rRNA同mRNA一樣,都是染色體DNA的轉錄產物。
  4. The expression profile of trpt1 was also examined using mtn blot from clontech. the trpt1 was highly expressed in skeletal muscle and in heart, but lower or undetectable in other tissues assayed. we thus suggested that the trna splicing may exhibit tissue specificity

    據此,我們推測;人體內的兩種trna剪接方式可能也存在著組織差異,即:種剪接方式存在於某些組織中,而另一種剪接途徑可能存在於其它組織中。
  5. Polymyositis ( pm ) and dermatoyositis ( dm ), devastating inflammatory muscle diseases, are associated with the myositis - specific anti - jo - 1 autoantibody, found in 25 - 40 % of such patients. anti - jo - 1 antibodies react with histidyl - trna synthetase

    抗組氨酰? trna合成酶抗體,臨床上又稱抗jo - 1抗體,是診斷這類疾病的標志性抗體。
  6. The immediate transcript of a trna gene is known as precursor trna.

    TRNA基因的直接轉錄物叫做tRNA前體。
  7. Scolopendra multilan is a common species belonging to scolopendromorpha, chilopoda. the sequence of scolopendra multilan mtdna determined is 11700 bp in length, about 70 % of the complete sequence. the sequenced portion contains 2 rrna genes, 1 a + t - rich region, 8 protein - coding genes and 18 trna genes

    本文對少棘蜈蚣的線粒體基因組進行了研究,已經測定的序列長11700bp ,約為全長序列的70 ,包含了2個rrna基因、 1個at富含區、 8個蛋白基因、 18個trna基因。
  8. Our previous work identified delayed mutation occurred at target supf trna gene in plasmid transfected into cells pretreated with low concentration of alkylating agent n - methyl - n ' - nitro - n - nitrosoguanidine ( mnng )

    在收回的質粒上我們檢測到了延遲發生的、高於對照5倍以上的突變。由於w在細胞中的半壽期僅為1
  9. The morphological diagnostic characters for many mature and early instar larvae are still lacking in china. partial sequences of the mitochondrial cytochrome oxydase i and ii and a transfor rna ( co i co ii and trna ) gene of the adults and larvae from caddisflies were. lepidostomaflavum, l. fui, l. arcuatum, paraphlegopteryx morsei, apsilochorema unculatum and apsilochorema hwangi, and the larval and adult stages of these species were sequenced and associated

    採用dnastarpackage中的editseq軟體進行序列編輯、 orf查找;採用clustalx軟體進行序列比對( alignment ) ;比對結果輸入mega2 . 1軟體計算各樣品間的遺傳距離,並基於kjmura2 - parameter模型,用鄰接法( neighbor - jojning , nj )構建系統發生樹,通過自展( bootstrap1000次)檢驗獲得系統樹分支的置信度。
  10. A page gel, stained by the petiodic acid - schiff ' s method to reveal glycoproteins, further displayed that the bindng - protein was a glycoprotein belonging to lectin, which contained 17. 4 % ( w / w ) neutral carbohydrate content of the glycoprotein detected by the phenol / h2so4 method. peptide mapping was comparable to the reported protein in protein bank. the database homology search ( ncbi blast ) indicated that the binding - protein shared 70 - 80 % homogeneity to l - aspartate oxidase, aspartyl / glutamyl - trna ( asn / gln ) amidotransferase subunit b, glutamyl - trna reductase, histidyl - trna synthetase

    連續梯度聚丙烯耽胺凝膠電泳、 sds一聚丙烯酞胺凝膠電泳和等電聚焦的結果表明該蛋白分子量為1 「 . skda ,由二個相同的亞基組成,亞基分子量為」 . ikda ,等電點為8 . 25 .糖蛋白染色結合考馬斯亮藍染色的結果證實此結合蛋白是個糖蛋白,其中糖含量為17 . 44 % ,蛋白含量為82 . 56 % .凝集反應確定該糖蛋白也是一個凝集素
  11. In our laboratory, a unique mutation detection system using a shuttle vector plasmid has been established to demonstrate that a low concentration of mnng ( 0. 2 m ) can induce nontargeted mutation in mammalian cells : the mammalian cells were exposed to 0. 2m mnng for 2. 5h, then a shuttle plasmid pz189 carrying supf trna gene was transfected into cells after 24h culture. we found a 5 - fold higher mutation frequency of the plasmid replicated in pretreated cells than the spontaneous mutation frequency of the plasmid replicated in control cells. this kind of mutation did not occur immediately after mnng exposure

    我們實驗室曾用一特殊的突變檢測系統,直接證明dna損傷劑可在哺乳動物細胞誘發非定標性突變:首先用低濃度( 0 . 2 m )的短壽烷化劑mnng (半壽期為1 . 1hr )處理細胞2 . 5h后,繼續培養24h ,將重組有用作突變檢測的靶基因supftrna基因的穿梭質粒pz189轉入細胞復制,發現在未受致癌物直接攻擊的穿梭質粒中有較自發突變率高5倍以上的靶基因突變。
  12. Lin s x, wang q, wang y l. interactions between escherichia coliarginyl trna sythetase and its substrates, biochemistry. 1988, 27 : 6348

    汪靜英,王應睞.琥珀酸脫氫酶的研究- -琥珀酸脫氫酶還原細胞色素c的性質.生物化學與生物物理學報. 1981 . 13 : 347 - 352
  13. The partial sequences of the mitochondrial co i, co ii and trna gene of the adults and larvae of 4 species from lepidostomatidae and the adults and larvae of apsilochorema unculatum and a. hwangi were sequenced and compared in this study. in the sequences obtained ( 1718 ~ 2239bp ), a % + t % was about 69. 2 %, 267 nucleotide sites were substituted in apsilochorema and 266 nucleotide sites were substituted in lepidostomatidae. at bias in the third codon position site was much higher than the other two sites, reaching about 81. 9 %

    {的比對分析表明:在coi區的一45一個比對位點中,有1260個保守位點, 191個變異位點,其中98個轉換位點, 93個顛換位點;發生在密碼子第三、第一和第二位點的變異分別為78 % 、 18 %和4 % ;種內序列歧異度為o一0 . 9 % ,而種間為一4 %一24 % 。
  14. Direct damage on supf trna gene can be neglected because half - life of mnng is 1. 1 hour and the interval between treatment and transfection was as long as 12 - 24 hours. therefore the mutagenesis is not lesion directed

    20m的mnng經洗滌后的極微量殘留經過10 ? 20多個半衰期的降解后,己不足攻擊轉人的qdna分子,因此這種突變顯然是發生在mnng直接攻擊部位以外的正常堿基上。
  15. Meanwhile, base composition in rrna genes, the secondary structrues of trna and the potential stem - loop structure in the noncoding region were also analysesed in the present study

    同時分析了d1na基因的堿基組成、 trna的二級結構、非編碼區潛在的莖環結構。
  16. Amino acids combined in trna complexes may be regarded as sequestered, or compartmented.

    結合於tRNA復合物中的氨基酸也可以認為是互不相關的,或者是被分室隔開的。
  17. Pit13, an interactor binding to trpt1 protein, was screened by yeast two - hybrid, and confirmed by pull down and co - ip assays. the association of trpt1 with pit 13 demonstrated that trpt1 should probably participate in other activities besides in pre - trna splicing. more experiments will be required to determine the role of pit 13 protein

    0酵母以雜交篩選、加衛工加wn檢測及ip實驗均證明m卜1 」且與功能未知的pit13蛋; 」之間存在著相互作用,說明imj基因除了參與trna剪接之外,還可能具有其它方面的功能,有待進一步研究。
  18. The results show that there are 7 strains hpi positive except 1 strain hpi negative. hpi in 6 strains of 7 hpi - positive strains is inserted into asnt - trna site. the expression of fyua gene, which encoded fyua, the receptor of ybt, was upregulated by extracellular ybt level

    研究結果提示,除1株eaggec中國分離株hpi 」外,其餘7株均為hpi且7株中有6株攜帶的hpi毒力島均插入在染色體的asnttrna位點。
  19. The immediate transcript of a trna gene is known as precursor trna

    Trna基因的直接轉錄物叫做trna前體。
  20. Hisityl - trna synthetase catalyzes the aminoacylation of trnahis in the initial step of protein biosynthesis. the involumen of histidyl - trna synthetase in autoimmune diseases is another feature of the enzyme. in studies, it is reported that purified jo - 1 antigen can increase the detection rate of anti - jo - 1 antibody. but it is difficult to obtain a single component by biochemical extraction. genetic engineering can help us to desolve this problem. after looking up mrna sequence encoding histidyl - trna synthetase in genebank, we used rt - pcr technology to gain its full length dna sequence. the vestor ptybllwas used in the construction of expressing vestor. we transformed the jo - 1 gene into er2566 and used this system to express fusion jo - 1 antigen

    本實驗從人胎盤中提取總rna ,利用rt - pcr技術獲得了編碼jo - 1的基因整長序列,選用impact - cn系統中的ptyb11載體,構建了jo - 1基因的克隆與表達載體,並轉化大腸桿菌er2566 ,經過抗性篩選、分子量大小比較、雙酶切鑒定、和pcr鑒定等多種方法驗證,篩選出了5個陽性克隆。
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