upstream restriction 中文意思是什麼
upstream restriction
解釋
上流節流- upstream : adv. 向上游，溯流。adj. 1. 溯流而上的。2. （石油工業等）上游的，和石油的勘探、開采及輸送到起運港口有關的〈石油工業此後的提煉等過程則稱為下游（downstream）產業〉。
- restriction : n. 1. 限制，限定。2. 拘束，束縛；自製。3. 【邏輯學】限定。
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In this study, we designed a pair of primers based on the sequence of the upstream and downstream of chicken il - 2 gene. about 600 bp chicken il - 2 cdna fragment was cloned from cona - stimulated chicken splenocytes by reverse transcription - polymerase chain reaction ( rt - pcr ) and was subcloned into puc18 vector. recombinant clone was demonstrated by restriction enzyme digestion and dna sequencing. next, we construct recombinant plasmid pproex ? t - il - 2. the cdna of chicken il - 2 gene was subcloned into bamh i / hind iii sites of vector. the recombinant plasmid pproex ? t - il - 2 was transformed into e. coli dh5a and the bacteria was induced with iptg. it was demonstrated by sds - page and western blot that a 18kda protein which was equal to chicken il - 2 protein in molecular weight was expressed in e. coli dh5a. the expression level was up to 30 % of the total bacterial proteins. the purified protein was used to prepare the antibody against chicken il - 2 protein
經酶切鑒定及dna序列測定，該基因為雞il - 2基因，其序列與sundick等報道的完全一致。在此基礎上，我們把雞il - 2基因亞克隆到大腸桿菌原核表達載體pproex ~ （ tm ） ht中，構建重組表達質粒並進行確證性序列測定，重組質粒測序結果表明將編碼雞il - 2成熟蛋白的基因正確地插入到原核表達載體pproex ~ （ tm ） ht的目的位點。重組質粒轉化受體菌dh5後用iptg於37進行誘導培養， sds - page和westernblot分析顯示，表達的雞il - 2融合蛋白分子量約為18kda ，表達的融合蛋白經薄層掃描發現目的蛋白表達量約占菌體蛋白的30 。 -
Over 13 kb saci restriction fragment was cloned into pgem - 7zf ( + ), mapped for restriction endonuclease sites and an about 5. 0kb fragment was further subcloned and sequenced. through coding region specific primer, we amplied it ' s corresponding cdna, named st901. st901 is 2889bp long, contains 1447bp putative promoter region within 5 " upstream and three exons ( 475bp, 140bp, 39bp ) and two introns ( 472bp, 2s3bp ) in the coding region, encodes a hydrophilic protein of 217 amino acid residues with a molecular mass of 24kda
以同源探針篩選馬鈴薯基因組文庫，得到四倍體馬鈴薯基因組dna ? st901 ，基因全長2889bp ，含3個外顯子（長度分別為475bp ， 140bp ， 39bp ）和2個內含子（長度分別為472bp ， 253bp ） ； 5 』端含有1447bp的啟動子區段，該區段具備一般啟動子的基本元件tatabox和caatbox ； 3 』非編碼區長63bp ，具hind酶切位點，沒有發現保守的加尾信號。
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