v gene 中文意思是什麼

v gene 解釋
v 區基因,v 基因
  • v :
  • gene : n. 【生物學】基因。 dominant gene顯性基因。
  1. Cluster analysis based on rapd showed that at 76 % similarity level, all tested isolated could be clusted into nine groups, i. e. 1 p. ostreatus and p. florida, ii p. ostreatm p. sapidus, p. spodoleucus and p. eryngii, iii p. colnmbinus, p. corlicalus, p. cornucopiae, p. nebrodensis and p. ferulae ; iv p. pulmonarius and p. sajor - caju, v three isolates with indefinite species, vi p. luber - regium, vii p. cilrinopileatus, viii p. djamor and p. salmoneoslramincus, ix p. abalonus and p. cysliodism. 4. a single uniform product 1. 46kb in size resulted from pcr amplification of the 5 " half of the 28s rrna gene for all isolates of pleurolus and the other three genera

    隨機擴增多態性dna的聚類分析表明，在76相似水平下，可將供試的側耳菌株聚成九大類，第一大類包括糙皮側耳、佛羅里達側耳；第二大類包括美味側耳、灰白側耳、剌芹側耳；第三大類包括哥倫比亞側耳、裂皮側耳、黃白側耳、阿魏蘑、白阿魏蘑；第四大類包括肺形側耳和鳳尾菇；第五大類為3株未定名的側耳；第六大類僅有具核側耳；第七大類為金頂側耳；第八大類包括紅平菇和桃紅側耳；第九大類包括鮑魚菇和囊蓋側耳。
  2. In this paper, a field strain of infectious bronchitis virus was isolated from proventriculus tissue, morphological observation by electron - microscope and the biological characterizations of the virus were studied, pairs of specific primers are designed and synthesized in correspondence with them, according to the published sequences of infectious bronchitis virus three structural protein ( spike protein s membrane protein m nucleocapsid protein n ) genes, the cdna of si gene, s2 gene, m gene. n gene of ib v isolate lx4 were amplified by rt - pcr and full sequences were first reported

    在此基礎上，根據國內外已發表的ibv基因序列，分別設計特異性引物，應用不同引物進行反轉錄合成cdna ，分片段對ibv的主要結構基因進行pcr擴增，並分別將各個目的片段克隆到puc19載體上，在大腸桿菌dh5中實現目的基因的分子克隆，經藍白斑篩選、限制性內切酶分析、 pcr鑒定，篩選出重組陽性質粒，並對各個目的基因片段進行序列測定，從而獲得ibv主要結構基因全序列。
  3. Are the changes in signal transduction and gene expression related to the structural alteration of the sugar chain on some surface receptors resulting from the transfection with sense or antisense gnt - v

    而細胞信號轉導的改變是否與細胞表面某些受體的糖鏈結構的改變（由gnt - v的正義或反義cdna轉染引起的）有關
  4. Human gnt - v contains 741 amino acids with six potential sites for n - glycosylation and bears high homology to gnt - v of rat. its gene is located on chromosome 2q21 containing 17 exons. gnt - v protein is encoded by exons 2 - 17 as open reading frame

    人類gnt - v由741個氨基酸組成，有6個潛在的n -糖基化位點，基因定位於染色體2q21 ，含有17個外顯子，其開放閱讀框架由外顯子2 - 17進行編碼。
  5. In the presented study, using h7721 human hepatocarcinoma cell line transfected with sense or antisense cdna of gnt - v, the effects of gnt - v on signal transduction an d its mechanism as well as the alteration of gene expression were investigated. we expected to elucidate whether and how the transfected glycosyltransferase modulate the cell signal transduction and gene expression

    本文以穩定轉染gnt - v正義或反義cdna質粒的h7721人肝癌細胞為材料，從下列四個方面對gnt - v影響信號轉導及其機制以及引起基因表達的變化進行了研究，企圖闡明糖基轉移酶轉染是怎樣調節細胞信號轉導的
  6. There are four imperfect repeats v - v - e - k - k - n / e - e of which the core sequence is similar to map ib of mouse. rna blot and rt - pcr analysis showed that this gene is expressed specifically in the mature pollen and can be classified as a late gene in pollen development

    計算機軟體分析st901基因核苷酸、氨基酸序列的相似性，結果表明， st901基因編碼區與探針sb401 、與高賴氨酸基因sblr 、與番茄tsb具有較高的相似性，它們可能來源於馬鈴薯的一個基因家族。
  7. Using the v. harveyi luminescence bioassay, expression of the luxs gene was detected in transformed tobacco. induced defence responses were observed after inoculated with plant viruses

    利用哈氏弧菌生物發光實驗檢測到該基因在煙草中得到表達，並通過病毒接種實驗觀測到轉化煙草被誘導的抗性反應。
  8. Culture supernatants of bt strains induced luminescence in v. harveyi reporter strain bb170, which can only response to ai - 2 signal moleculars, indicating that the bt strains have the luxs gene. by aligning the nucleotide sequences of the b. anthracis and b. cereus luxs genes, a pair of primers were designed and an 474 - bp fragment was amplified from bt strains by pcr

    首先通過生物發光實驗檢測到蘇雲金芽孢桿菌（ bacillusthuringiensis ， bt ）的培養液可以誘導特異性檢測ai - 2信號分子的哈氏弧菌報告菌株bb170發光，表明bt中含有群體感應信號分子ai - 2的合成基因luxs 。
  9. In e coli, dna polymerases are key enzymes involved in two distinguished pathways contributing to untargeted mutagenesis. replication of dna by pol v ( umuc ), in the presence of umud1, reca and single strand binding protein ( ssb ), is highly mutagenic and exhibits a predominant mutation pattern of base transversion. another error prone polymerase involved in untargeted mutagenesis is pol iv, encoded by dinb gene

    在umud ' ， reca和單鏈結合蛋白ssb的協助下， polv （ umuc ）能在單鏈模板上催化dna合成並產生高頻率的以堿基顛換為主要形式的突變；另一個與非定標性突變有關的易誤dna聚合酶是pol ，為dinb基因的編碼產物。
  10. Methods : the rearranged gene fragment coding tcr y v region of the jurkat cell line was obtained by rt - pcr technique the pcr product was cloned into the eukaryocytic expressive vector pcdnas to construct pcdna3 / tcr y. after confirmed by sequncing. pcdnas / tcr y plasmids were amplified in bacteria extracted by alkaline lysismethod

    方法：本文採用rt ? ? pcr的方法擴增jurkatt淋巴瘤細胞特異性重排的tcr可變區基因片段，克隆到真表達載體pcdna _ 3中，經序列測定無誤后，堿裂解法大量提取質粒，制備dna疫苗。
  11. This expression vector plbcas - hsa - lgl has the following advantages : i ) the 1. 7kb promoter is able to drive cell - specific and hormone - dependent expression ; ii ) the inclusion of intron - 1 can increase expression level of fusion genes ; iii ) the 5 ' utr of bovine p - casein mrna may have a positive role in both transcriptional and post - transcriptional regulation ; iv ) the gfp gene make the selection of positive clone among embryos possible ; v ) the gfp gene can be easily excised via cre - mediated recombination between the two loxp sites after the expression vector has been integrated into chromosome ; vi ) the two incompatible lox sites, loxp and lox2272, would facilitate cre - recombinase mediated cassette exchange ( rmce ), which in theory will leading to develop a technology of site - specific gene expression in animal mammary glands

    該載體的特點是：具有可以調控外源基因在乳腺中特異表達的牛-酪蛋白基因5 `端側翼區和包括第一外顯子及內含子在內的5 `端調控區；將人血清白蛋白cdna準確地置於牛-酪蛋白基因第二外顯子中的翻譯起始密碼子atg之後，而且沒有增加額外的序列和使人血清白蛋白cdna移碼；引入標記基因gfp ，便於在胚胎期鑒定陽性胚胎，減少受體；引入cre lox重組系統： （ ? ）標記基因gfp的兩端的兩個loxp位點可以在表達載體整合到基因組后，刪除標記基因； （ ? ）餘下的一個loxp位點可以和前面的lox2272位點組成cre重組酶介導的盒式交換系統。
  12. According to the cdna of hwtx - v, hwtx - v and mhwtx - v might be come from the same gene. the difference of two toxins may be result from post - translation modification

    根據hwtx -的cdna分析，我們認為這兩個毒素來自於同一個基因，是經過翻譯后加工形成的天然突變體。
  13. Then some necessary elements such as gfp gene, neo gene were inserted between the loxp site and the loxs 11 site and obtained a new gene targeting vector pioxgfp. on the other hand, the chicken v - ifn cdna was cloned into the vector plox between the loxp site and the lox511 site and got another gene targeting vector ploxifn

    合成loxp和lox511位點，將其同方向地克隆到載體pegfp - 1的多克隆位點之內，構建成含有lox位點的通用載體? ? plox ，然後將報告基因gfp及neo基因片段克隆到載體plox上的loxp和lox511序列之間，構建成一基因打靶載體? ploxgfp 。
  14. Under low ph conditions, vde converts violaxanthin ( v ) to a and z within minutes. in this study, we not only cloned genes encoding vde enzyme from rice and spinach, but also transferred svde gene into tobacco

    紫黃質脫環氧化酶（ vde ）是葉黃素循環的關鍵酶，在較低的ph條件下，它能在數分鐘內將紫黃質（ v ）轉變為z和a 。
  15. Comparing with database in genbank, the results showed ej175 had 56 % homology to mrna for a known pollen allergen - like protein in arabidopsis thaliana. ej175 was proved to encode gene of pathogenesis - related protein bet v i family by conserve domain analysis

    對該基因可讀框編碼的蛋白進行結構功能域分析，證實了該基因4 - 147位氨基酸存在病程關聯蛋白betvi家族（ pathogenesis - relatedproteinbetvifamily ）的的保守結構域bet - v - i 。
  16. Polymorphism of bovine tlr2 gene digested with ecor v and its associations with somatic cell score

    酶切多態性與體細胞評分的關聯分析
  17. Svde gene was also amplified from spinach by rt - pcr. the plant expression vector pcb - antisvde was constructed, in which svde gene was controlled by camv35s promoter and nos terminator. antisense transgenic tobacco plants v. ere obtained from leave explants via agrobacterium tumefaciens - medialed transformation

    從菠菜中克隆了svde基因，並構建了該基因的反義抑制植物表達載體pcb - antisvde ，用根癌農桿菌介導法轉化煙草，獲得了大量的轉基因植株。
  18. First, mrna was purified from the total rna extracted from fresh spleens of nonimmunized mice of kunming, then the cdna library was achieved via reverse transcription pcr. gene fragments encoding v

    首先從未免疫小鼠的脾臟中提取總rna ，分離出mrna ，經逆轉錄合成其總cdna 。
  19. Conclusion : the target gene of the domain iv of the chain v 2 could be gained from keratinocytes and expressed in prokaryotic cells in high efficiency

    結論： ln - 5 2鏈功能區基因可從皮膚組織中獲得，並在原核細胞中得到高效表達。
  20. So through gene engineering technology, we clone v - src gene into pgex - kt, expressed in e. coli tgi and dh5 a after transformation and iptg induction

    因此，我們設想在大腸桿菌中表達v - src蛋白，填補目前研究的一些缺陷。
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