viral genome 中文意思是什麼
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It refers to the release of the viral genome from its protective capsid to enable the nucleic acid to be transported within the cell and transcribed to form new progeny virions
它指的是將病毒基因組從它的保護性衣殼中釋放出來，使核酸能在細胞內轉運並能轉錄以形成新的子代病毒。 -
The homology of the other two motifs ( v and vi ), which are also quite conserved in other helicases, were lower than 30 %. the hasn pv helicase protein had considerable amino acid sequence similarity with other baulovirus helicases ( 58 % ), and the highest ( 66 % ) with semnpv and the lowest with xcgv. ( 43 % ). hasnpv helicase was the first helicase reported in single - nucleocapsid nucleopolyhedrovirus there are 5 homologue regions ( hrs ) in hasnpv genome which may play important roles in the viral replication
同源性比較發現hasnpv解螺旋酶的氨基酸序列與甜菜夜蛾核多角體病毒（ spodopteraexiguemnpv ， semnpv ）的解螺旋酶具有最高的同源性（ 66 ） ，與xestiac - nigrum顆粒體病毒（ xcgv ）解螺旋酶同源性最低（ 43 ） ， hasnpv解螺旋酶基因是第一個報道的單粒包埋核多角體病毒的解螺旋酶基因。 -
2. an up - dated method was employed to purify tumv in this research. using the protease k method, we acquired the viral genome - rna. a pair of specific primers was designed and synthesized based on the nucleotide sequences of tumv coat protein genes reported before and rt - pcr was used to clone the cp genes of the six tumv isolates
應用改進的蕪菁花葉病毒的提取方法從病葉中提取病毒粒體，應用蛋白酶k法從病毒粒體中提取病毒rna基因組，根據已報道的tumv的cp基因序列，設計併合成了一對特異引物，利用rt - pcr法克隆了6個分離物的外殼蛋白基因，與克隆載體puc19連接后通過熱激法轉化大腸桿菌dh5 。 -
In order to investigate the genomic organization of the single - nucleocapid nucleopolyhedrovirus of helicoverpa armigera, the ecori - n fragment located at 54. 8 - 59. 3 kbp of the viral genome was sequenced. the fragment contained 3762 bp helicase gene potentially encoding a protein with a molecular mass of 146 kda
對棉鈴蟲單核衣殼核多角體病毒（ helicoverpaarmigerdsingle - nucleocapsidnucleopolyhedrovirus ， hasnpv ）基因組中ecori ? n片段進行序列分析，獲得了完整的解螺旋酶基因（ hel ） ，其開放閱讀框大小為3762bp ，編碼一個分子量為146kda的蛋白質。 -
Chapter two is a report on genetic organization of the hindlll - l fragment of hasnpv. the fragment that is 7501 bp long and located at map units ( mu ) 12. 3 - 17. 9 of the viral genome was sequenced
對棉鈴蟲單核衣殼核多角體病毒（ hasnpv ）的研究歷史和近期對hasnpv的基因組學研究以及本論文的研究內容作了簡要介紹。 -
The close genetic relationship of goose parvoviruse and aav allows the examination of the molecular biological properties of the nonstructural proteins of gpv. after the gpv infected the cell the viral life cycle was regulated by the nonstructural proteins encoded by the virus. according to the published of gpv b strain genome nucleotide sequences in genbank and a pair of specific primers were disigned with oligo4. 1
本研究根據genbank發表的gpvb株全基因序列，藉助oligo4 . 1軟體設計一對引物，採用pcr技術擴增gpvh1株非結構蛋白ns2基因，並與pmd18 - t載體連接后測序，結果表明：鵝細小病毒h1株ns2基因核苷酸全長1356bp ，編碼451個氨基酸殘基，與gpvb株的ns2基因相比，核苷酸數目相同，有17個堿基、 6個氨基酸的差異；同源性分析表明：二者核苷酸序列同源性為98 . 75 ，推導氨基酸序列同源性為98 . 67 。 -
Plasmid pucis was used to construct viral genome library. ten fragments were obtained and some of them were partially sequenced and analysed using genbank data
利用puc18質粒構建了織線藻病毒部分基因組的dna文庫，獲得了10個基因組片段，作了部分克隆片段的序列測序和分析。 -
But it is doubt that the speciality and stability for the outcome of detecting bdv orf i genome. we will detect the indviduals ' ( include the neuropsychiatric 4 patients with bdv orfii postive or not and healthy blood donator ) middle fragment of bdv orf i to help defmiting whether internal healthy people and the certain neuropsychiatric patient such as with viral encephalitis, multiple, schizophrenia, affective disorder have catch a bdv infection or not, most of all, the research is for understanding if there is any difference in the fragment from animal and human beings infected with bdv, thereby to find another sensitive and stably marker for confirming bdv infection, and establish a solide bottom for advanced study
但對另一編碼p40蛋白序列的檢測結果的特異性和穩定性還不肯定，我們通過對先前檢測bdvorf基因片段為陽性或陰性的神經精神病人和健康獻血者進行了bdvorf基因中部片段的檢測。以幫助確定在國內健康人群和某些神經精神疾病患者中如：病毒性腦炎、多發性硬化、精神分裂癥以及情感障礙等疾病中是否存在bdv的感染，更為重要的是了解感染人和動物的該病毒片段是否存在差異，從而找出另一檢測bdv感染的敏感性和穩定性的標記物，為下一步的研究奠定良好的基礎。 -
There were six major open reading frames in genome of f48e9 strain which encode the viral np, p, m, f, hn and l protein, respectively
為了驗證該6個堿基是否只存在於強毒株及其與毒力的關系，我們又設計一對引物x1和x2 。 -
Sequence and phylogenetic analysis of segement a of chinese infectious bursal disease virus ( ibdv ) hb - bp strain isolated from hebei province was studied in this thesis. the experiments as below were included : double - strand rna of viral genome was purified by licl gradient precipitation, 48 hours after bursa was harvested from chicken which had been inoculated with hb - bp strain. referred to the published sequence two primers were designed and synthesized
給4周齡spf雛雞人工接種雞傳染性法氏囊病病毒hb - bp毒株， 48小時后採取法氏囊，用雙licl分級沉澱法提純全長基因組dsrna ，設計一對引物，通過rt - pcr方法進行了體外擴增，獲得hb株a節段基因全長cdna 。 -
The centre of excellence, located at the hepatitis research laboratory in the prince of wales hospital, can offer viral genome sequencing for patients who receive anti - viral therapy
中心於威爾斯親王醫院的肝炎研究實驗室，可以為接受抗病毒治療的乙肝病人進行病毒基因組排序。 -
There are many mutations leading to drug resistance exist in the viral genome which varies in different individuals especially in those receiving different anti - viral drugs
不同乙肝病人在接受不同程度的抗病毒藥物治療后，病毒會出現各式各樣的變異，導致病毒基因組產生抗藥性。
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