virulence of the virus 中文意思是什麼

virulence of the virus 解釋
病毒毒性
  • virulence : n. 1. 有毒，毒力，毒性。2. 刻毒，惡毒，惡意。3. 【醫學】病毒性；致病力。
  • of : OF ＝Old French 古法語。
  • the : 〈代表用法〉…那樣的東西，…那種東西。1 〈用單數普通名詞代表它的一類時（所謂代表的單數）〉 （a） 〈...
  • virus : n 1 【醫學】病毒；濾過性病原體。2 毒素；毒害。3 惡意，惡毒。4 【計算機】（電腦）病毒〈指擾亂或破...
  1. In trpsin tolerance assay. this virus could resist to 1 % trpsis at 37 in an hour. in acid tolerance assay, this virus was resistant to ph3. 0 and ph5. 0 at 37 in 2 hours, and the average infection litre of the virus decreased little. in heat assay, at 50, the virus was processed from 5 minutes to 150 minutes and at each condition the viral virulence reduced to some certain degree. among these conditions, when at 50 in 30 minutes. the average infection litre of this virus decreased over 2 tilre. and when al 50 in an hour, cpe of ihis virus disappeared. when time was set for an hour. but with processed in different temperature as 50 60 70, 80, the virus losl the multiplication capacity complelely. in biological assay, we selected different cell lines to cultivate this virus by laking advantage of possesional cells at that time in our laboratory. then we found that fcwf cell line was the most sensitive to dxmv and mdck was the second. with f81 cell line, after passaged for 12 times continuously with low concentration of fcs. the virus could produce cpe. however, with vero cell line. the virus could not procuce any cpe after many passages. the hemagglutination and lumadsorption reaction test proved that this virus had no any reaction to erythrocyte of pig, fowl and cavy. by neutrolizaion assay, dxmv could be identified as a kind of ccv

    理化學研究表明，該病毒為rna病毒，對氯仿、乙醚敏感；胰酶試驗中，經37 、 1小時處理的病毒，仍然能夠在貓源細胞fcwf細胞上生長，並且毒力基本保持不變；耐酸性試驗中，病毒分別在ph5 . 0和ph3 . 0經37作用2小時，毒力僅下降一個滴度；耐熱性試驗中，該病毒在恆定溫度50 ，設定不同時間，從5分鐘到150分鐘，毒力均有不同程度下降，其中， 50作用30分鐘，病毒平均滴度下降2個單位； 50 ， 60分鐘， cpe消失；恆定時間1小時，設定不同溫度（ 50 - 60 - 70 - 80 ） ，病毒在細胞上完全喪失增殖能力， cpe消失。生物學試驗，利用實驗室現有條件，選擇不同的細胞系對該病毒進行培養，發現該病毒對貓源細胞fcwf最敏感； mdck細胞次之； f81細胞經多次傳代，亦可出現cpe ；而vero細胞則不敏感。血凝試驗表明，該病毒對豬、雞、人及豚鼠的紅細胞均無血凝性。
  2. By morphologic examination, structure characteristics ^ biological property n serological tests and pathology characteristic study, this virus was identified as the medium virulence chicken. new castle disease virus ( ndv ), whose pathology characteristics were appeared different from those of the standard virulence ndv. la sola vaccine could not give good protection against the infection of this virus isolate

    用spf雞胚在吉林省患病雞群中分離到一株病毒，經病毒形態學、結構特徵、生物學、血清學及致病性等試驗研究，確定該病毒為雞新城疫強毒，對lasota免疫雞的致病性及其致死率與雞新城疫標準強毒e _ 9f _ （ 48 ）對比差異顯著， lasota疫苗對該株病毒免疫保護效果不理想。
  3. Newcastle disease virus ( ndv ) is grouped under the family of paramyxoviridae, it causes serious infectious disease and incurred great losses to poultry industry. f and hn protein were main glycoproteins, which affect the virulence of the virus

    新城疫病毒（ newcastlediseasevirus ， ndv ）屬于副粘病毒科（ paramyxoviridae ）副粘病毒亞科（ paramyxovirinae ）腮腺炎病毒屬（ rubulavirus ）的成員，其主要結構蛋白融合蛋白（ f ）和血凝素-神經氨酸酶（ hn ）與其致病性密切相關。
  4. Avian paramyxovirus type 1 ( apmv - 1 ) may cause diseases, named differently in different poultry species, including chicken newcastle disease, goose paramyxovirus disease, pigeon paramyxovirus type 1 disease. the virulence and pathogenicity of apmv - 1 field isolates are different among the poultry species. there is not a technique available for the rapid diagnosis of the disease in all the species and the determination of virulence of the virus at the same time

    禽型副粘病毒（ avianparamyxovirustype1 ， apmv - 1 ）引起的相關疾病主要有雞新城疫、鵝副粘病毒病、鴿型副粘病毒病，但不同禽源的apmv - 1分離株對其它禽類的毒力和致病性都有所差異，雖然apmv - 1在多種禽類廣泛流行，目前仍未有一種既可檢測所有禽源的apmv - 1 ，同時又能鑒別其毒力強弱的快速診斷方法。
  5. This strain ' s virulence was judged by mean death time of chick embryos ( mdt ), intracerebral pathogenicity index in day - old chicks ( icpi ) and intravenous pathogenicity index in 6 - week - old chickens ( ivpi ) and it was found to be the virulent strain. at last, it was tested by the recurrent infection and found that it was the newcastle disease virus ( ndv ), and it was named hbg - 1 strain. in order to find the difference of the cleavage site of this strain with f48e9 and ? 30 strain, a part of the cleavage site of fusion protein gene fragment was amplified by rt - pcr using a primer and sequenced. the sequence analysis showed this strain had low homology with f4ge9 and cso. a phylogenetic tree based on the published sequences of ndv reference strains was constructed and showed the isolated strain hbg - 1 belonged to the genotype w ndv, a novel genotype ndv

    為了進一步探尋分離株與標準株的異同，又採用rt - pcr方法，擴增獲得分離株f _ o裂解位點附近的基因片段，經測序后與國際上已發表的新城疫病毒的核酸序列進行比較，結果表明其與標準株和疫苗株之間的同源性較低，僅為82 86之間。經系統發育進化樹分析后，判定該分離株為新城疫病毒（ ndv ）基因型。運用計算機軟體對其裂解位點處的氨基酸序列進行預測和分析，結果表明該分離株為新城疫病毒強毒株並具有基因型的典型結構特徵。
  6. Two amino acid substitutions near the vp2 - vp4 cleavage site were identified in the virulent strain jz2000 and might be involved in increased virulence of the virus. phylogenetic analyses indicated that these two strains were most closely related to some european strains but distinct from wibdv and variant strains

    分子系統進化樹分析表明， zj2000株和jd1株與cu - 1 、 p2 、 cef94 、 harbin等毒株的關系最近，而與歐洲、香港、日本的超強毒株和美國的變異株相對較遠。
  7. The fusion ( f ) protein and hemagglutinin - neuraminidase ( hn ) are the major virulence factors of the virus to which the host responds well with protective antibody production. recombinant plasmids containing the f or hn gene can induce specific immune responses against ndv. however, dna vaccination by injection or gene gun routes is rather inconvenient and costly in field applications in scaled animal farms

    融合蛋白（ f ）和血凝素-神經氨酸酶蛋白（ hn ）是ndv的主要宿主保護性抗原，以f基因和hn基因為免疫原研製的dna疫苗能誘導一定程度的免疫應答，但注射免疫或基因槍免疫途徑因實際操作和成本等問題難以推廣應用，而直介面服重組質粒dna免疫效果不理想。
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