virulent gene 中文意思是什麼
virulent gene
解釋
毒性基因-
In recent years, the outbreak of nd appear some new characters, it is reported that the amount of immunity defeat on nd increased, even chicks with high hi titer can be infected by ndv virulent strain. the gene - engineering vaccine is badly needed to control nd outbreak. so newcastle disease virus strain b95, a naturally avirulent and heat stable strain, was introduced from australia
新城疫是國際獸疫局公布的16種烈性傳染病之一。近年來，新城疫的爆發出現了許多新特點，免疫失敗報道增多，甚至抗體水平較高的雞群也發病。為控制新城疫的爆發，急需研製基因工程苗。 -
Pseudorabies virus ( prv ) is the causative agent of aujeszky ' s disease, which results in significant losses in pig husbandry. thymidine kinase ( tk ) gene is one of the main virulent genes of prv, and it is essential to the propagation of prv in the nerve tissues, but dispensable for virus replication and infection in other tissues
偽狂犬病是由偽狂犬病病毒（ pseudorabiesvirus ， prv ）引起的家畜和多種野生動物的一種以發熱、奇癢、呼吸和神經系統疾病為特徵的急性傳染病，妊娠母豬可發生死胎和流產，是危害養豬業的一個重要傳染病。 -
Researchers found that high concentrations of salt in the stomach appear to induce gene activity in the ulcer - causing helicobacter pylori bacterium that causes it to become more virulent
研究人員發現，胃中高濃度的鹽可以誘導致潰瘍性幽門螺旋桿菌的基因活性，從而使其更具危害性。 -
This strain ' s virulence was judged by mean death time of chick embryos ( mdt ), intracerebral pathogenicity index in day - old chicks ( icpi ) and intravenous pathogenicity index in 6 - week - old chickens ( ivpi ) and it was found to be the virulent strain. at last, it was tested by the recurrent infection and found that it was the newcastle disease virus ( ndv ), and it was named hbg - 1 strain. in order to find the difference of the cleavage site of this strain with f48e9 and ? 30 strain, a part of the cleavage site of fusion protein gene fragment was amplified by rt - pcr using a primer and sequenced. the sequence analysis showed this strain had low homology with f4ge9 and cso. a phylogenetic tree based on the published sequences of ndv reference strains was constructed and showed the isolated strain hbg - 1 belonged to the genotype w ndv, a novel genotype ndv
為了進一步探尋分離株與標準株的異同，又採用rt - pcr方法，擴增獲得分離株f _ o裂解位點附近的基因片段，經測序后與國際上已發表的新城疫病毒的核酸序列進行比較，結果表明其與標準株和疫苗株之間的同源性較低，僅為82 86之間。經系統發育進化樹分析后，判定該分離株為新城疫病毒（ ndv ）基因型。運用計算機軟體對其裂解位點處的氨基酸序列進行預測和分析，結果表明該分離株為新城疫病毒強毒株並具有基因型的典型結構特徵。 -
The comparison of meq gene sequences of different pathotypes of marek ' s disease virus meq genes of different pathotypes of marek ' s disease virus ( mdv ) were amplified, in the whole opening reading frame ( orf ) by polymerase chain reaction ( pcr ) technique, sequenced and compared with the published sequence of ga strain ( representing vmdv ). cvi988 / rispens and 814, commercial vaccine strains popularly used worldwide and in china respectively ; 648a, representing very virulent plus ( vv + mdv ) and 6 field isolates, originated from guangxi commercial chickens with visceral lymphomas and vaccine breaks, representing high pathogenic ( hpmdv ) and two of them ( g2 and n ) were proved to be vvmdv, were used
本研究通過三個方面進行了研究，取得了如下主要實驗結果： 1mdv不同致病型（ pathotypes ）毒株meq基因序列的比較研究應用pcr技術擴增了不同致病型mdvs ，即國際通用型疫苗毒cv1988 rispens株和中國特有的疫苗毒814株、特超強毒648a株（ vv + mdv ）以及6個廣西分離到的野毒株共9個毒株的meq基因，進行核苷酸序列的測定並與標準強毒ga株（ vmdv ）進行核苷酸和氨基酸序列的比較。 -
The gene was 1668bp in length, encoding the f protein composed of 553 amino acids. sequence analysis and its secondary structure prediction showed that the f protein had three major hydrophobic regions consisting of about 25 amino acids, six potential glycosylation sites and thirteen cysteines. a sequence region of basic amino acids, rrqrrf, was found at the f, - f2 cleavage site, indicating that f4ge9 was a typical virulent strain
序列分析和二級結構預測表明， f _ （ 48 ） e _ （ 9 ）株f蛋白含有3個分別由25個氨基酸組成的疏水區，存在6個潛在的糖基化位點， 13個半胱氨酸殘基，裂解位點區域的氨基酸序列為rrqrrf ，說明f _ （ 48 ） e _ 9株是一株典型的強毒株。 -
Four mcabs were developed and can detect the meq gene expression in the cef infected with oncogenic mdvs and naturally occurred md tumor cells by fa and immunohistochemistry technique respectively, but ca n ' t detect that in the cef and normal chicken liver tissue respectively infected with non - virulent vaccine virus cvi988 / rispens. the results demonstrated that meq is highly expressed in t
獲得4株穩定產生抗meq蛋白mcab的雜交瘤細胞，其中3g12e6單抗通過fa和免疫酶組化技術能夠檢測到mdv致瘤株感染的cef及自然md腫瘤細胞中表達的me叮基因產物，而在非致瘤株cvi988 / rispens感染的cef和免疫的正常雞的組織中則未檢測到。 -
Detection of specific gene and analysis of virulent gene of e. coli o157 h
7特異基因檢測與毒力基因分析 -
The virulent gene after analyzing two closely related strains of the h5n1 obtained from infected geese in southern guangdong province in 1996 - one highly pathogenic in chickens and the other harmless
研究人員分析了1996年從廣東省的受感染鵝身上抽取的兩種關系密切的h5n1病毒，致力於研究該病毒的基因。 -
A reverse - transcriptase polymerase chain reaction ( rt - pcr ) based technique was developed to detect newcastle disease virus ( ndv ) of different poultry species origin. four oligonucleotide primers, based on the differences of nucleotide sequence at the cleavage site of fusion ( f ) protein gene between virulent and non - virulent strains of apmv - 1, were designed to amplify specific dna fragment from different viruses
依據apmv - 1融合蛋白（ f ）基因裂解位點的核苷酸序列與其毒力的相關規律，分別設計合成了四條寡核苷酸引物，建立了一個可迅速檢測不同禽源apmv - 1並可鑒定強、弱毒株的逆轉錄酶?聚合酶鏈式反應（ rt - pcr ）技術。 -
Infectious bursal disease virus has been a great concern for the poultry industry for a long time, particularly for the past decade when its " re - emergence " in variant or highly virulent forms. in this study, two sets of primers ( pta and pts, ibda and ibds ), flanking the hyper - variable region of vp2 gene, were designed to run a reverse transcription polymerase chain reaction ( primary rt - pcr ) and nested - pcr. both of these assays can amplify all of 12 reference strains which including pathotypes cibdv, vvibdv and vibdv, but not the 5 negative reference pathogens of chicken
結果12個參考毒株均能擴增出約679bp的目的片段，而陰性對照的常見5種雞病病原：雞新城疫病毒（ ndv ） 、雞傳染性支氣管炎病毒（ ibv ） 、雞傳染性貧血病毒（ caiv ） 、大腸桿菌和多殺性巴氏桿菌均沒有擴增到任何片段；應用建立的技術對疑似ibd的34份臨床病料進行檢測，並同時在基礎rt - pcr擴增的片段內設計另一對引物進行nested - pcr 。
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