virus culture 中文意思是什麼
virus culture
解釋
病毒培養-
Tissue culture is the most important tool used in virus assay.
組織培養是用於病毒試驗的最重要的手段。 -
It is an important that bacteria contaminated vaccine in the biologicals production. we collected 703 samples of cell culture, virus cultivation and harvest which were contaminated by bacteria during poliovaccine production within two years. we checked these samples by bacteriological method and antibiotics sensitivity tests were done. it shows that 1 ) the main contaminated bacteria come from staphylococci, bacilli and streptococci of environment in the poliovaccine production. 2 ） it is effect that antibiotics to contaminated bacteria are doxycycline, albiotic, prescription 2, cefotaxime na salt, gentamycin, neomycin, aureomycin and erythromycin
在疫苗生產實踐中，細菌污染是影響疫苗質量和產量的關鍵性因素，筆者通過了兩年左右的時間，選取正常生產中零星細菌污染的細胞培養瓶、病毒培養瓶及收毒污染樣品等共703份，進行細菌學檢查，並對造成污染的主要細菌種類進行了各種抗菌藥物的耐藥性實驗，結果表明：我所脊灰疫苗生產中主要的污染威脅來自環境中的葡萄球菌，潛在威脅是桿菌和鏈球菌；強力黴素、林可黴素、配方2 、噻孢黴素鈉鹽、慶大黴素、新黴素、金黴素和紅黴素等抗生素對目前引起污染優勢細菌-葡萄球菌有明顯的抑菌效果，可作為疫苗生產后備抗菌手段參考 -
A pair of primers were chemically synthesized based on the cdna sequence of hog cholera virus strain brescia and used to amplify the cdna fragments of envelope glycoprotein e2 gene of hclv which coding the major protective antigen by reverse transcription - polymerase chain reaction ( rt - pcr ) from total intact rna extracted from infected calf testicle cell culture by hclv
以豬瘟兔化弱毒犢牛睪丸細胞毒（ hclv ）中提取的細胞總rna為模板，以rt - pcr技術，擴增出了完整e2基因的cdna片段。經電泳、酶切分析，證實了所擴增片段為e2基因特異性片段。 -
Shoot tip culture for elimination virus in hot pepper
辣椒莖尖培養脫毒研究 -
Research advance in technique of sweet potato meristem tip culture for virus - free
外源激素對甘薯不同品種莖尖培養與植株再生的影響 -
The supernate of virus culture was used as templet in overlapping pcr, then the interesting gene was obtained
利用重組pcr的方法，以病毒培養液上清為模板，把二個目的基因串聯在一起。 -
Sds - page results showed that there was a clear target protein band in mut + recombinant supernatant after 48 hours of culturing, while a faint band only in muts recombinant after 72 hours. western - blotting result showed that there was no remarkable difference of yield between mut + and muts recombinants after 6 days induced. anti - virus activity tests revealed that culture supernatants of mut + and muts recombinants could inhibit tmv infection with high efficiency in the same concentration and there was no significant difference between them
結果表明，誘導培養48小時后， mut ~ +重組菌株表達產物在sds - page膠上顯現出清晰的目的蛋白帶，而mut ~ s重組菌株培養72小時才能顯示微弱的目的帶； western - blotting雜交信號強度表明，同樣培養6天的mut ~ +和mut ~ s重組菌株表達產物在表達量上沒有明顯差別。 -
Cucumber mosaic virus resistant mutant from tobacco ' s anthers treated with ray through anther culture
輻射與花藥培養相結合篩選煙草抗黃瓜花葉病毒病突變體 -
In the adherent culture, the virus did not result in cell lysis after the insect cells were infected
而在本論文中，我們嘗試將此一非溶裂昆蟲細胞/桿狀病毒表現系統轉移到旋轉瓶的懸浮培養。 -
In order to identifiy the virus further, a set of double nested primers for canine coronavirus was selected. the primers were designed in s gene region from ccv including two pairs of primers : ccvfl - ccvrl, ccvf2 - ccvr2. the first is a pair of outer primer, and can amplify a fragement of 1086bp. the second is a pair of inner primer. and can amplify a fragement of 515bp. using the nested primers, many ccv strains can be identificated including k378, insave - l, ccv 1 - 71 etc. synthesizing this set of primers, we selected the panda ' s liver - tissue materials and some different passages of viral culture to amplify by rt - pcr, and all of them respectively gained two target fragements of 1086bp and 515bp, but the control cell did not
合成該套式引物，選擇大熊貓原代病料和病毒各代細胞培養物，經套式（ nested ） rt一pcr擴增，可得到一與設計值5巧bp相符的dna片段，經bst一xl （ 590 ， 1110 ）酶切鑒定，證明該擴增片斷為特異性片段;回收大熊貓肝組織原代病料和細胞培養物第2 、 3 、 29代的ccvfz一ccvrz擴增片段，純化，送生物公司測序。 -
Culture of the attenuated a66 strain of duck hepatitis virus in chicken embryo fibroblast
66弱毒株在雞胚成纖維細胞上的培養 -
An infectious eiav clone was recovered by transfecting fatal donkey dermal ( fdd ) cell cultures and donkey leukocyte ( dl ) culture in vitro with the full - length gene clone of dv. the virus ( designed pd70344v ) derived from the third passage in dl culture was observed by electron microscope and the reverse transcriptase ( rt ) activity was determined
將包含全基因片段的三個基因克隆以限制性內切酶消化后順次連接克隆到載體ptz18r上，構建了4個全長基因的分子克隆，分別命名為pd30343 、 pd70333 、 pd70343 、 pd70344 ，其中兩個轉移到低拷貝載體plg338上，命名為plgd30343 、 plgd70344 。 -
The eiav - pok8. 2 - his was transfected into the donkey leukocyte culture. following an incubation period, reverse transcriptase activity was detected in cell culture supernatants. cytopathic effect was observed by no. six passages post - infection in donkey leukocyte infected by the virus derived from eiav molecular clones eiav - pok8. 2 - his
提取前病毒dna ， pcr擴增p1p4片段，亞克隆至pmd18 - t載體，測序證明前病毒dna含有六個組氨酸，從而在體外獲得了感染性克隆病毒株。 -
Twenty years later scientists found that a virus that causes the veterinary disorder newcastle disease shows a preference for infecting tumor cells and began to try to enhance that tendency by growing the viruses for generations in human cancer cells in laboratory culture dishes
20年後，科學家發現引起新城雞瘟的病毒，特別偏愛感染癌細胞，因此他們在實驗室里的人類癌細胞株中培養此種病毒，試圖加強這種傾向。 -
In vitro axenic culture of a strain of trichomonas vaginalis carried with virus
陰道毛滴蟲攜病毒株體外純培養 -
The pbacph - egf plasmid dna was used to co - transfect bmn cell with the modified bombyx mori baculovirus bm - bacpak dna, which was first linearized by aocl. after two rounds of plaque isolation, the recombinant virus ( named bmbacph - egf ) was further verified with pcr and dot hybridization. the recombinant bmbacph - egf virus was used to infect bmn culture cells at a moi of 10
重組轉移載體dna與經bsu36i酶切線性化的修飾型病毒bm - bacpakdna共轉染家蠶bmn細胞，兩輪空斑篩選和純化，獲得重組病毒rbmbacph - egf ，經pcr擴增、 dna點雜交等方法鑒定證實重組病毒中已正確插入ph - egf融合基因。 -
No trace of any newly expressed protein band was noticed in supernant as well as in the cells by sds - page, except the verification of the substitution of beta - galactosidase gene ( the lose of galactosidase protein band ), which is a selective marker of the wild - type virus. elisa test results suggested the expression of egf in cells, but not in culture supernant. the quantitative calculation suggested the expressed egf was about 6 - 7 u g ( as egf standard ) per flask ( 2 > < 106 cells ) in the cellular extract
將重組病毒rbmbacph - egf以10moi感染bmn細胞， 72小時后收集培養細胞和上清；培養上清和經超聲波處理的細胞樣品elisa檢測發現胞內樣品中存在能與egf抗體免疫反應的產物，粗略估計表達量約6 7 g 2 10 ~ 6個細胞（相當于egf標準品） ；重組病毒rbmbacph - egf穿刺接種5齡家蠶幼蟲，每隔24h收集蠶血淋巴，經elisa檢測發現第4天表達量最高，根據egf標準曲線計算蠶血淋巴的表達量約32 g ml ； elisa定性實驗還發現正常蠶血也存在與egf抗體間交叉反應的物質。 -
The paper overviews the formation and characteristics of the technique of rapid propagation of free virus in plant tissue culture, and its application in flower, wood, fruit tree, vegetable. . etc., and introduces the main technical link to produce the seedling, including the function and choice of media, the principle and need of donor plants, tame method and transplant request of plant, productive plan of seedling and budge means of cost
摘要綜述了植物離體快速繁殖技術和脫毒技術的形成、特點及其在花卉、林木、果樹、蔬菜等方面的應用，闡述了利用快繁與脫毒技術生產種苗的主要技術環節，包括培養基的作用和選配要點、外植體選取的原則和快繁與脫毒的不同要求、試管苗馴化的方法與移栽要求、種苗生產計劃的制定與成本預算方法。 -
The biogas research institute under the the ministry of agriculture, and the pengzhou bureau of agriculture. he investigated some issues in sichuan such as the tissue culture of virus - free seed potato, technology extension and construction of energy in the rural areas
農業部沼氣科學研究所以及彭州市農業局，就四川在脫毒馬鈴薯種薯組織培養技術推廣以及農村能源建設等問題進行了調研，同時也分別向四川的相關部門工作人員介紹了加拿大脫毒種薯生產體系建設生物能源研究等方面的情況。 -
" these five farms, which are located close to the earlier - infected farms, had been on our suspicion list since february 5. they had been quarantined to facilitate our staff to conduct a thorough investigation. after detailed examination and laboratory testing including virus culture, they proved to be infected
他說：這五個雞場位於較早時被證實受感染的雞場附近，署方因此懷疑這些場內的雞只的健康可能出現問題，在二月五日起將農場隔離，以便本署職員進行徹底調查。
分享友人