western blot analysis 中文意思是什麼
western blot analysis
解釋
蛋白印跡分析-
The western blot analysis show that the antiserum induced by the new antigen plus hemocyanin could bind with the 22kd and 55kd proteins, which were existed in the testis tissue protein of mouse, rat and human. 2 ) the purified peptide emulsified in an equal volume of freund ' s adjuvant and immunized the female balb / c mice with 8 - 10 weeks old. the antiserums and the washings of vaginal membrane were detected by elisa, and shown the highest level of the specific antibody igg was 1 : 6000, while the iga was 1 : 300
二、 p3多肽與弗氏佐劑混懸后免疫近交系balb c小鼠后，在血清和陰道粘膜沖洗液中可檢測出特異性lgg 、 lga ，最高效價分別達到1 ： 6000和1 ： 300 ；免疫后的小鼠脾臟淋巴細胞增殖率升高，淋巴細胞培養上清液中分泌的il 4和inf y也升高，且以il 4更明顯。 -
The cells were harvested and total e. coli proteins were detected by sds - page to select expressed strains. western - blot analysis of the gel that showed a band of similar size was the major protein immunoreactive with the anti - 6xhis mab
Rpoifn經部分純化並充分復性后，用細胞病變抑製法測定rpoifn的抗濾泡性口炎病毒（ vsv ）活性。 -
Salt treatment had effects on growth, succulence and some physiological parameters. in present study, suaeda salsa seedlings were treated with different salts and isoosmotic peg to examine the succulence and some physiological parameters. the hydraulic conductance ( lo ) of the roots, the water permeability of protoplasts and western blot analysis of aquaporins in plasma membrane and tonoplast under nacl were determined
本實驗以鹽生植物堿蓬幼苗為材料，用不同的鹽及與nacl等滲的peg處理，測定肉質化及有關生理指標，並測定nacl處理下植物根的導水性，原生質體的水滲透性，並在分子水平上進行了細胞質膜及液泡膜水孔蛋白免疫雜交分析。 -
To investigate whether ha 132 is a structural component of hasnpv, western blot analysis of proteins in budded viruses ( bvs ) and occlusion derived virions ( odvs ) was conducted
對來自bv和odv的總蛋白質進行westernblot分析，結果表明ha132蛋白不是hasnpv病毒粒子的結構成份。 -
The positive colonies that grew on the ampicillin ( amp ) plate ( lb agar medium contaning 100 g / ml amp ) were screened and identified. sds - page and western blot analysis were performed to study the expression profiles of target gene cein in e. coli
從轉化的平板中篩選出陽性重組子，進行不同iptg濃度和不同誘導時間的表達研究，並利用sds - page電泳和westernblotting蛋白質印跡技術對外源基因cei _ （ 12 ）在大腸桿菌e -
Get the inclusion bodies 2 ) western blot analysis of fusion protein expression ( 1 ) electrophoresis ( 2 ) transfer proteins from gel to membrane ( 3 ) blocking ( 4 ) incubation with primary antibody ( 5 ) enzyme conjugate incubation ( 6 ) substrate incubation. 3 ) gst detection module with cdnb enzymatic assay 3 purification of gst fusion proteins 1 the denaturalization of inclusion bodies. 2 purification using glutathione sepharose 4b column wash matrix with 1 pbs, prepare a 50 % slurry for batch purification method, pack column with matrix slurry
三、 gst一hbrp重組蛋白的純化1 .超聲破碎細胞，離心，上清和沉澱進行sds一page電泳分析2 .樣品處理提取包涵體，變性后，加人用pbs平衡過的以utal腸onesepb抓脫4b ，室溫下孵育3 .以utadtionesepharose4b柱純化以utad雲onesepharose4b柱的準備根據毛山lel ，決定純化所需的gll衛ta1如oneseph娜se4b的柱床體積，用預冷的1xpbs清洗cldtathionese戶， se4b ，得到50 %的基質 -
Rt - pcr results suggested ha94 was a late gene. western blot analysis of extracts of hasnpv - infected hzaml cells revealed a specific protein of 43 kda from 48 h to 96 h p. i.
Rt - pcr結果表明ha94是一個晚期基因，在感染后24小時開始檢測到病毒的轉錄產物，持續到表達至96小時。 -
Western - blot analysis showed the expressed protein was specific to antisera against prv fa strain. the ge - elisa for differentiation of prv infected from vaccination was developed using the expressed ge protein as antigen
Coli中高效表達的偽狂犬病病毒ge蛋白為抗原，以辣根過氧化物酶（ hrp ）標記的spa為二抗，建立了ge - elisa鑒別診斷方法。 -
To investigate the functions of these factors, cells were treated with anti - c - fos, anti - c - jun and anti - ras antibodies and examined microscopically for the cell cycle progression. the results indicated that c - fos - like protein, c - jun - like protein and ras - like protein played important roles in checkpoint regulation in physarum polycephalum. western blot analysis showed that the c - fos - like protein and c - jun - like protein may act in cell cycle checkpoint by changing the cyclin b1 - like protein and p53 - like protein expression ; and the ras - like protein may act by changing the cyclin bl - like protein, p53 - like protein, c - fos - like protein and c - jun - like protein expression
蛋白免疫印跡分析的結果表iv明，當s期、 gz期和前期細胞內具有功能活性的類ras蛋白的量減少時，細胞中的細胞周期調控因於類cyclinbi蛋白、類c jun蛋白、類c fos蛋白和類p53蛋白的表達水平，與未經抗體處理時相比發生了變化（或升高或下降） ；而山抗體處理引起的多頭絨泡菌細胞內具有功能活性的類c fos和類c jun蛋白的減少，使細胞內類cyclinbi蛋白和類p53蛋白的表達水平與抗體未處理時相比也發生了明顯的改變。 -
The secretive expression of scfv fusion proteins were confirmed by sds - page and western blot analysis in cell split products and condensed supernatant
建系細胞裂解產物和濃縮的培養上清經sds page及westernblot分析，證實了scfv融合蛋白的分泌性表達。 -
Western blot analysis demonstrates that it reacts positively with goat antibody
Western - blot鑒定表達產物有特異的抗原抗體反應。 -
With ni2 + - lda - sepharose, the cbd tag was removed. according to the results of sds - page and western blot analysis, the product of outflow was the target protein lt a 27. the purity of lt a 27 was about 95 %
運用niz +金屬鰲合親和層析柱進一步快速純化去除融合頭，上樣流出經sds一隊ge分析和western印跡分析ii1 :明得到的純化產物為淋巴毒素缺失體蛋白lt凸27 ， lt 2 :產物純度可達95 %以上。 -
The protein has the immunogenicity of human calcitonin verified by western blot analysis. after optimization of condition, fusion protein was concentrated by ultrafiltration and cleaved by factor x a, then recombinant novel hct was obtained
表達條件優化后，可溶性蛋白的表達量占胞內總蛋白的10 。經超濾濃縮后，融合蛋白在xa因子作用下產生重組的新型降鈣素，進一步分離純化得到該蛋白的初級純品。 -
27kd protein bands were detected in transgenic plants by western blot analysis, and the results showed that the foreign lea3 gene could express in transgenic strawberry plants
Westernblot分析，在轉化植株中檢測到了27kd雜交帶，表明在轉化草莓體內有外源基因翻譯產物的積累。 -
Western blot analysis we treated 200 fertilized eggs in a series of steps including lysing them in l0ul of lysis buffer ( 50mm tris - hcl ph 7. 5, 250mm nacl, 5mm edta, imm dtt, 0. 1 % triton, 50mm sodium orthovanadate, looug / mg pmsf and tpck, 50ug / ml tlck, 1 ug / ml leupeptin pepstatin and aprotinin ), frozing them below - 70, and then thawing them at room temperature for 10 min
2 . westem印跡將處理組及對照組小鼠一細胞g2期受精卵各200個取出，轉移到ep - pendoff管中， 5000甲m離心4分鐘，棄去上清，加入ro川粉碎緩沖液，充分振蕩混勻，在液氮中經過2一3次的凍融循環，迫使卵細胞裂解，加人等量的樣品緩沖液沸水中煮5分鐘，用於westem印跡分析。 -
Western blot analysis showed that rhpk - 5 ( recombinant human plasminogen kringle 5, rhpk - 5 ) protein was recognized by mab same as native hpk - 5. the result suggested that we obtained correct gene sequence of hpk - 5 and got the purified rhpk - 5. section ii : construction of pbv220 / hpk - 5 vector for obtaining high - level hpk - 5 expression system, the hpk - 5 gene was recombined with plasmid pbv220 to construct the vector of pbv / hpk - 5
Coli ）作為宿主，經sds - page分析，篩選表達量最高的菌株作為發酵用工程菌株；用western - blot方法鑒定hpk - 5因子的免疫學活性；用搖瓶發酵的方法，研究發酵培養基的體積（溶解氧） 、組成成份及誘導起始時間和誘導持續時間對目的蛋白表達量的影響，優化hpk - 5基因工程菌的表達條件。 -
By northern and western blot analysis, we examined expression pattern of vfcpk1 in different parts including roots, stems, leaves, mesophylls and epidermal peels in broad bean at mrna or protein levels, and effects of different external stress treatments on vfcpkl
通過northem和westernblot分析，我們研究了蠶豆鈣依賴蛋白激酶vfcpk1在蠶豆不同部位包括根、莖、葉、葉肉和下表皮中的mrna和蛋白質水平上的表達情況以及不同外界脅迫處理對vfcpk1表達的影響。 -
The elisa and western blot analysis indicated that the reaction between annserum and antigen was high specifically. to block the annfaf expression in strawbern. " fruit, the antisense gene of annfaf
為獲得annfaf基因的表達缺陷型植株，以研究該基因在草莓果實成熟過程中的調控作用，本研究構建了annfaf反義融合基因的植物重組表達質粒prok - annfaf 。 -
To investigate whether orf94 is a structural component of hasnpv, western blot analysis of proteins in budded viruses ( bvs ) and occlusion derived virions ( odvs ) was conducted
對hasnpv感染的hzam1細胞的樣片進行westernblot分析，從感染后48h到96h檢測到一條43kda大小的特異蛋白質帶。 -
Western blot analysis of fractionated cell extracts reveals that mature mint is cleaved into approximately 110 kda ( n - terminal ) and approximately 250 kda ( c - terminal ) fragments
韓驛教授與thofljo研究組合作對小鼠的mtwt進行了基因剔除，對dopartmentofmedicalgenet 。 。
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