western blot assay 中文意思是什麼
western blot assay
解釋
西式吸印雜交試驗-
Get the inclusion bodies 2 ) western blot analysis of fusion protein expression ( 1 ) electrophoresis ( 2 ) transfer proteins from gel to membrane ( 3 ) blocking ( 4 ) incubation with primary antibody ( 5 ) enzyme conjugate incubation ( 6 ) substrate incubation. 3 ) gst detection module with cdnb enzymatic assay 3 purification of gst fusion proteins 1 the denaturalization of inclusion bodies. 2 purification using glutathione sepharose 4b column wash matrix with 1 pbs, prepare a 50 % slurry for batch purification method, pack column with matrix slurry
三、 gst一hbrp重組蛋白的純化1 .超聲破碎細胞，離心，上清和沉澱進行sds一page電泳分析2 .樣品處理提取包涵體，變性后，加人用pbs平衡過的以utal腸onesepb抓脫4b ，室溫下孵育3 .以utadtionesepharose4b柱純化以utad雲onesepharose4b柱的準備根據毛山lel ，決定純化所需的gll衛ta1如oneseph娜se4b的柱床體積，用預冷的1xpbs清洗cldtathionese戶， se4b ，得到50 %的基質 -
In order to study the expression of 3 - defensins in liver as acute phase response proteins, a murine systemic acute phase responsive model was established by intraperitoneal injection o f lipopolysaccharide ( lps ) in our study. the mbd3 cdna sequence ( 145 - 169 bp ) was labled with [ - 32p ] atp as a probe to detect mbd3 mrna in different tissues by northern blot and analyze the time - and dose - dependant expression caused by lps. the 5 " flanking sequence ( - 167 - 179 bp ) of the mbd3 gene was designed as the probe and labled with [ - 32p ] dctp to investigate the binding of transcriptional factors to this region by electrophoretic mobility shift assay ( emsa ) and south - western blot
以小鼠mbd3基因145 ? 169bpcdna序列合成探針，經[ - ~ （ 32 ） p ] atp標記后通過northernblot方法檢測mbd3在肝臟中的表達，同時分析了mbd3基因誘導表達的組織特異性，劑效和時效關系；結合mbd3基因啟動子區序列分析，以- 164 ? - 179bp雙鏈dna序列合成探針，經[ - ~ （ 32 ） p ] dctp標記后通過電泳遷移率改變實驗（ emsa ）和south ? westernblot方法對參與mbd3在肝臟中誘導表達調節的轉錄因子進行分析。 -
Was first processed by dgd embedment and embedment - free technique and general technique for em morphology. a perinuclear structure consisted of interacted filaments we called lamina - like structure was observed. then using western blot assay, we found a lamin - like component band of 68kd protein in the three - step - fractionated cells. to investigate the distribution of the lamin - like protein in cells, a immunofluorescence experiment for in situ hybridization was designed using goat anti - lamin protein antibodies as the probes. the results revealed that the positive reactivity presented at different part of the cells. the perinuclear cross - actions were distinct, and cross - action with the oral apparatus and the cortex were also obtained
本文以dgd包埋去包埋技術對草履蟲的核纖層通過透射電鏡和免疫熒光分子雜交等技術進行了觀察。結果顯示，在核周存在由10nm纖維組成的核纖層免疫熒光結果表明，在核周呈陽性反應，並在其表皮口器等部位呈交叉的陽性反應蛋白分子雜交的反應帶在68kd處呈陽性反應。 -
The activity of the antiserum was tested by dot enzyme immunization assay ( diba ) with purified antigen. western blot of hela nuclear protein extract, which contained natural hbaf53, showed that the antiserum is also a specific antiserum to natural hbaf53. therefore the highly specific and sensitive antiserum can be applied to various studies
用純化的抗原蛋白，經斑點印跡試驗測得baf53抗血清的效價，又用提取的hela細胞核蛋白（含有天然baf53蛋白）進行免疫印跡分析（ westernblotting ） ，證明天然baf53蛋白也是該抗血清的抗原，說明獲得的多抗血清具有高特異性和敏感性，可用於多方面的研究。 -
Sds - page and western - blot assay show that the expressed product was 29kda and could be recognized by anti - 6his mcab
Page和westernblot鑒定，獲得原核表達的mbl蛋白。 -
Blood samples from 12, 000 hong kong citizens were collected and screened for the presence of the sars - cov antibody using enzyme - linked immunosorbent assay ( elisa ). positive samples underwent immunoflourescence assay ( ifa ) and western blot for confirmation
經初步酵素連結免疫吸附分析法（ elisa ）測試呈陽性的樣本更會透過免疫螢光法分析法（ immunofluorescenceassay ） ，及西方點墨法（ westernblot ）化驗以作最終確定抗體的存在。 -
Western blot assay using purified chloroplast confirmed that only cp was existed in it
通過葉綠體的western - blot檢測亦證明病毒粒子在葉綠體中的存在，而vp37蛋白在葉綠體中分佈較少。
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