western hybridization 中文意思是什麼

western hybridization 解釋
蛋白質雜交
  • western : adj 1 西的，西方的；在西的；向西的；從西方來的。2 〈W 〉 西洋的，西歐的。3 日落西山的，衰頹的。n ...
  • hybridization : 混成作用
  1. Nogo - 66 receptor, ngr, cloned in 2001, is a leucine - rich - repeat glycophosphatidylinositol - anchored membrane protein which mediates nogo - 66 inhibition of axonal outgrowth. both the long acidic amino - terminal domain and the nogo - 66 fragment have strong neurite growth inhibitory activity suggest that nogo - a has at least two inhibitory domains. northern blot, in situ hybridization, western blot and immunocytochemistry analyses show that in addition to oligodendrocytes, nogo - a mrna and nogo - a protein are also expressed in neurons in developing and adult brain and spinal cord, nogo - a is also found in peripheral organs such as heart and testis

    Northernblot 、原位雜交、 westernblot和免疫組化結果證明： nogo amrna和nogo蛋白除了在cns的寡突膠質細胞中表達，還表達于發育階段和成年的腦、脊髓和外周神經節的某些神經元中，在外周組織如睪丸和心臟也有表達； nogoe在cns和pns以及多種外周組織中有廣泛分佈； nogo （除表達于腦和心臟外，在骨骼肌中有較高表達。
  2. The localization and expression of prolactin receptor from inner mongolia alpas cashmere goat were studied by sacpic staining, in situ hybridization and western blotting. samples of skin were taken at interval three months from birth, three months old, six months old, nine months old, ten months old or twelve months old, which correspond to summer, autumn, winter and spring. paraffin sections of hair follicles were stained with sacpic staining and in situ hybridization. the protein of prolatin receptor is abstracted from samples of skin in order to study on expression of prolactin receptor. there are prolactin receptors in outer root sheath, dermal papilla and inner root sheath. the growth of primary follicle is continuous

    本實驗從絨山羊出生后每隔三個月采一次皮樣，共分為4個月齡（ 3 、 6 、 9 、 10或12 ）段，通過製作石蠟切片，原位雜交、染色，並提取皮樣蛋白做westernblotting等實驗研究方法，研究了催乳素受體mrna催乳素受體在不同生長季節的內蒙古阿爾巴斯白絨山羊皮膚毛囊中的定位與表達，染色結果發現阿爾巴斯白絨山羊初級毛囊全年持續生長，次級毛囊的生長情況隨季節而變化，秋冬季生長旺盛，夏季生長緩慢與絨毛生成規律呈正相關。
  3. Was first processed by dgd embedment and embedment - free technique and general technique for em morphology. a perinuclear structure consisted of interacted filaments we called lamina - like structure was observed. then using western blot assay, we found a lamin - like component band of 68kd protein in the three - step - fractionated cells. to investigate the distribution of the lamin - like protein in cells, a immunofluorescence experiment for in situ hybridization was designed using goat anti - lamin protein antibodies as the probes. the results revealed that the positive reactivity presented at different part of the cells. the perinuclear cross - actions were distinct, and cross - action with the oral apparatus and the cortex were also obtained

    本文以dgd包埋去包埋技術對草履蟲的核纖層通過透射電鏡和免疫熒光分子雜交等技術進行了觀察。結果顯示，在核周存在由10nm纖維組成的核纖層免疫熒光結果表明，在核周呈陽性反應，並在其表皮口器等部位呈交叉的陽性反應蛋白分子雜交的反應帶在68kd處呈陽性反應。
  4. In the first part of the present work, the expression changes of angiotensinogen ( agt ) and angiotensin - converting enzyme ( ace ) as well as its time course characteristics were investigated by rt - pcr, in situ hybridization and western blotting. we also prepared the polyclonal antiserum against agt for the following work

    故本工作應用rt - pcr和原位雜交、 western印跡分析等方法對模擬失重大鼠不同部位動脈血管血管緊張素原（ angiotensinogen ， agt ）及血管緊張素轉化酶（ angiotensin - convertingenzyme ， ace ）基因表達變化的時程特徵進行了觀察，並為后續工作制備了抗agt多克隆抗血清。
  5. The hybridization effect and important trait comparison of the litter sazi in taihu - pig and western - pig

    家豬基因組含有大量保守非編碼區
  6. In the present experiment studies, an acute traumatic model of lateral cortical impact was employed to study expressive changes of microtubule associated protein - 2 ( map - 2 ), cyclooxygenase - 2 ( cox - 2 ), glial cell line - derived neurotrophic factor ( gdnf ), caspase - 3 mrna and protein after brain injury in rats. immunocytochemical staining, western blotting, nucleic acid in situ hybridization with an oligonucleotide probe and computer image analysis were used to detect the dynamic changes of map - 2 mrna, cox - 2 mrna, gdnf mrna, and caspase - 3 mrna in the cortex after moderate traumatic brain injury ( tbi )

    本實驗從自行設計大鼠腦損傷落體打擊器開始，先行建立了一個便於觀察和施加處理因素、控制性好、重復性好的動物模型，選用30g擊錘從25cm高處下落，沖擊應力d為355 . 09kpa ，打擊大鼠右頂部，造成中等程度的閉合性腦損傷，從病理形態學、組織超微結構觀察及微管相關蛋白- 2 （ microtubuleassociatedprotein2 ， map - 2 ） 、環氧合酶- 2 （ cyclooxygenase2 ， cox - 2 ） 、膠質源性神經營養因子（ glialcellline - derivedneutrophicfactor ， gdnf ） 、 caspase - 3基因及蛋白表達的時間性變化，詳盡系統地闡述腦損傷后各指標變化的時間規律性及表達差異可能的形成機制。
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