wild type gene 中文意思是什麼
wild type gene
解釋
野生型基因-
All f1 seedlings showed wild - type salt - sensibility, and two of four mutants were detected that the proportion of the salt - tolerance seedlings to salt - sensibility seedlings was 1 : 3 in f2 progenys. the results of genetic analysis suggested that this mutants have a monogenic recessive mutation in a nuclear gene
F _ 1代白花授粉所得的f _ 2代中，兩個株系抗鹽植株與不抗鹽植株的比例為1 ： 3 ，表明該突變體是隱性單基因突變體。 -
Wild type gene
野生型基因 -
Preparation and identification of recombinant adenoviral vectors containing human wild type spk and its mutant gene
攜帶人鞘氨醇激酶及突變體基因重組腺病毒載體的制備及表達 -
Four out of 150 random primers could detect dna polymorphism between the two mutants and the wild type arabidopsis thaliana, which provided a strong evidence for the truth of the mutants. only one primer gave a specific dna fragment about 1200bp which two mutants own but the parent do n ' t. we drew a preliminary conclusion that the fragment might be related to the salt - tolerance gene
擬南芥抗鹽突變體的rapd分析結果表明， 150條引物中有四條引物在突變體和野生型擬南芥之間擴增出多態性，證明了dna水平突變的發生，其中1條引物在突變體的擴增產物比在野生型的擴增產物多出一個大小約為1200bp的片段，初步認為該片段可能與植物的抗鹽性有關。 -
Construction of recombinant fowlpox virus coexpressing aiv ha and ndv f. for the construction of transfer vector pfgs11haf, aiv ha gene of f strain in puc18ha and ndv f gene of f48e8 strain in puc19f were removed and inserted into pfgs11. recombinant fowlpox viruses ( rfpv ) coexpressing aiv ha gene and ndv f gene were constructed by using different promoters of ps and pe / l. recombinant rfpvs were derived by dosper liposome - mediated transfection with the two transfer vectors on chicken embryo fibroblast ( cef ) monolayer cultures which were infected by wild type fpv chinese vaccine strain 282e4 3 - 4 hours earlier
Puc18ha和sk質粒同時經hind 、 kpn酶切后連接得中間質粒skha ；將質粒skha用bamhi酶切回收ha基因插入到插入載體pfgs11中的bamhi位點，通過酶切鑒定獲得了pfgs11ha ；將含ndvf基因的質粒puc19f用hind 、 sal酶切經klenow酶補平插入到經sma酶切后的skifn中pe / l啟動子下獲得中間質粒skf ，再將質粒skf和puc18質粒先分別用ecor 、 xho酶切klenow補平，后再共同用sac酶切連接得puc18pelf ， sal酶切回收pe / l - f基因盒插入到pfgs11ha的sal位點，通過酶切鑒定獲得了pe / l - f與ps - ha同向的表達載體pfgs11haf 。 -
This has allowed the development of a balanced - lethal system for the expression of heterologous antigens in vaccine strains using vectors containing the wild - type asd gene from s. typhimurium and asd mutant salmonella hosts
Sds一page和westem一blot檢測表明，菌苗侯選株能夠分別表達出相對分子質量為64kda的尿素酶b蛋白與77kda的尿素酶b和熱休克蛋白a的融合蛋白。 -
Accordingly, ers2 - 1 is still able to confer ethylene insensitivity via a single receptor gene ers1 in the quadruple mutant, but at a highly reduced level compared to its function in the triple mutants. the major difference between the quadruple and triple mutants is the absence of a wild - type ers2 gene in the quadruple mutant, and we propose that the dominance conferred by ers2 - 1 can be mediated and amplified via the wild - type ers2 to the subfamily i receptor ers1
三突變體的遺傳背景與四突變體相比，只是在三突變體中保留了ers2的野生型基因，而當這個野生型的ers2基因突變后，對乙烯不敏感的ers2 - 1基因的功能便減弱了，說明顯性基因ers2 - 1在etr1 - 7 ； err2 - 3 ； ein4 - 4三突變體中的功能可以經由活化ers2后再傳給ers1 ，而不僅僅是直接傳給ers1 。 -
The future characterization and genetic analysis for candidate mutant were carried out and find that some candidate mutant ( such as roi30 doil - 1 doi0311131 ) have good phenotype by drought h2o2 aba - stressed treatment. at the same time we also observe the development of candidate mutant at different growth stages carefully. many modal difference between mutant an d wild type at the same period were found, such as more rosette layering fatty and big in leaves, advancment or delay for the flower period, rosettes living in the main stem, shorten in figure, the amount of seed little, sterilization etc. these physiological and modal changes may reflect with maladjustment in expressions of some gene and confusion on their inner control, . we will futher study concrete and detailed function mechanism
我們對這些擬南芥侯選突變體進行進一步的鑒定和遺傳學分析，發現ro口口、 doil 、 doi口jlll3i等潛在突變株對aba 、過氧化氫及早脅迫有明顯表型，同時對潛在突變體的生長發育進行了詳細的觀察，發現多數潛在突變株與同條件下野生型比出現了許多明顯的形態改變，如:蓮座基葉增多、分層、肥大，花期提前或延遲，主莖生輪座，株型矮化，產籽量少，不育，敗育等，這些生理和形態上的差異很可能反映了它們內部某些基因的表達受到了影響、代謝調控發生了紊亂，具體和詳細的作用機制還需要進一步的研究。 -
No trace of any newly expressed protein band was noticed in supernant as well as in the cells by sds - page, except the verification of the substitution of beta - galactosidase gene ( the lose of galactosidase protein band ), which is a selective marker of the wild - type virus. elisa test results suggested the expression of egf in cells, but not in culture supernant. the quantitative calculation suggested the expressed egf was about 6 - 7 u g ( as egf standard ) per flask ( 2 > < 106 cells ) in the cellular extract
將重組病毒rbmbacph - egf以10moi感染bmn細胞， 72小時后收集培養細胞和上清；培養上清和經超聲波處理的細胞樣品elisa檢測發現胞內樣品中存在能與egf抗體免疫反應的產物，粗略估計表達量約6 7 g 2 10 ~ 6個細胞（相當于egf標準品） ；重組病毒rbmbacph - egf穿刺接種5齡家蠶幼蟲，每隔24h收集蠶血淋巴，經elisa檢測發現第4天表達量最高，根據egf標準曲線計算蠶血淋巴的表達量約32 g ml ； elisa定性實驗還發現正常蠶血也存在與egf抗體間交叉反應的物質。 -
The photoabsorption properties of three kinds of br molecule films ( the wild - type br, the chemical enhanced br, the gene - variant br ) are investigated by measuring their absorption spectrum. using the gene - variant br film as a light - modulator, by the experiment of write - readout image and the analysis of image contrast, the light - modulated property of the modulator is studied. the relationship between wavelength of the modulated light and response time of the modulator is discussed emphatically
本論文概述了細菌視紫紅質分子結構、功能特性及研究發展的歷史與現狀，介紹了目前已有的和潛在的一些重要應用，通過對吸收光譜響應特性的測試研究了三類br分子薄膜（野生型，化學修飾型，基因修飾型）的光吸收特性，通過圖像的記錄/讀取實驗及圖像的對比度分析探討了基因改性型br分子薄膜的光調制特性，著重研究作為一個光調制器件對調制波長和調制時間的響應特性。 -
Our results provided direct evidence that pf40 gene play a role in the tissue development, with knockout this gene the lateral buds were not visible. the plant hormones auxin and cytokinin appear to play a major role in controlling this process, therefore, the concertrition of auxin and cytokinin was measured, however, the concentration of the two hormone was variation a little in transgenic plants, but the ratio of the two was more than 5 fold in the pf40 gained function plant than that in wild type. furthermore the pf40 gene effected differentiation of the vascular bundles in different transgenic plants, results showed the pf40 could change both xylary fibers and vessel
進一步的實驗發現pf40基因會影響植物側芽發育的早期，即pf40基因作用在側芽形成與發育的早期。對轉基因穀子的組織切片的分析還表明pf40基因引起不同形式的轉基因穀子的維管組織的發育的差異，轉化正義載體穀子的木質部變多，維管系統增多，而反義或干擾的轉基因穀子的木質部數量變少，木質部分化異常。從轉基因的結果可見pf40基因可以減弱植物的頂端優勢，這種作用是通過改變植物體內激素的含量實現的。 -
To prepare the wild type mbl in prokaryotic system, a pair of primers was designed and synthesized, and was used to amplify mbl gene from the recombinant vector pgem - mbl that contans wild type mbl cdna. a recombinant prokaryotic expression vector, pet28 - mbl, was constructed by inserted the mbl gene into plasmid pet28 ( b ), and after transfected it into ecoli bl21 ( de3 ) and induced with iptg, recombinant mbl protein was expressed successfully
本實驗另選用了原核表達質粒pet28 （ b ） ，根據已構建好的含有mbl野生型基因的t載體pgem - mbl ，設計一對引物， pcr擴增mbl基因，凝膠回收，雙酶切pcr產物和pet28 （ b ）質粒， t4連接酶連接，轉化大腸桿菌dhsa ，氨芋選擇培養挑取克隆鑒定。 -
Secondly another vector phz1117 which for replacement of sc ( j11. 17 / scd78. 01, was constructed. phz1117 was transformed into wild type strain m145 and a gene replacement strain named hxy3 was obtained
其次，構建了另一個用於置換scq11 . 17 scd78 . 01的置換載體phz1117 ，得到基因置換菌株hxy3並通過southern雜交驗證，與野生型m145相比，沒有觀察到hxy3有明顯的表型變化。 -
The expression and localization of wild - type p53 - gfp fused gene on human high - metastasis hepatocellular carcinoma cell line
熒光蛋白融合基因在高轉移人肝癌細胞中的表達與定位 -
Isolation and gene type detection of wild - type measles viruses in shaanxi province
陜西省麻疹野病毒的分離及基因分型 -
( 3 ) we further demonstrated that the atcull component of scfco11 complexes was modified via axr1. mutations axr1 resulted in reduction of the modified atcull component of scfcol1 complex. ( 4 ) the mutant alleles of axri ( axr ] - 3 and axr ] - 12 ) showed moderate insensitivity - to ja - inhibitory root growth and mild reduction of ja - inducible gene expression of atvsp, thi2. i and pdfi. 2 compared with that of wild type
以表達融合蛋白flag - coi1的axr1基因突變體axr1 - 3為材料，用- flag抗體進行免疫共沉澱分析發現， scf ~ （ coi1 ）復合體中修飾的atcul1蛋白的含量明顯降低，表明axr1基因突變影響對scf ~ （ coi1 ）復合體中修飾的atcul1蛋白的修飾。 -
Approximately 7000 colonies were screened. one mutant was obtained, and its enzyme activity is 2. 09 - fold higher than that of wild - type a - aldc in low temperature. the two nucleotide substitutions were occurred in the evolved a - aldc gene
根據定向進化的基本思想，我們利用易錯pcr的方法，篩選了近7000個菌落，最終得到了一株在低溫下也有較高酶活力的突變體。 -
( 2 ) compared with wild - type torenia fournier, antisense acc synthase gene transformated plants of torenia fournieri had the following changes : the rate of ethylene biosynthesis dramatically decreased in the plants. cholrophyll content in the leaves is 1. 15 times of that of the wild - type torenia fournier leaves. the transformed plants had more flowers
（ 2 ）反義acs轉基因藍豬耳與野生型的藍豬耳相比較，乙烯的生成速率顯著下降：葉片葉綠素的含量提高，是野生型的1 . 15倍；花開的更多，花的壽命增加一天；單個果實的種子的數量和重量均有所增加；葉片多胺的含量顯著高於野生型的藍豬耳；植株對氧化脅迫、酸脅迫、乾旱脅迫的抗性均有增加。 -
Whether the gene was transformed into the arabidopsis was confirmed by pcr - southern blotting, southern blotting and northern blotting. 1. pcr - southern blotting showed that all kanamycin - tolerant plants had strong positive signals, and no signal was shown in wild type plants
Pcr結果擴增出1 . 4kb的特異性條帶，表明sod2已整合進擬南芥中， pcr - southern進一步證實了pcr結果的正確性。 -
The result demonstrated that the ctbvpl fusion gene was inserted into the c. reinhardtii chloroplast genome and after three rounds of spc selection the recombinant chloroplast dnas were the majority, and the wild - type chlorop last dnas
W七sternblot和elisa免疫雜交分析表明ctbvpi融合蛋白在衣藻葉綠體中得到表達，表達量占可溶性總蛋白量的3一4 % （ lmg可溶性總蛋白含有30一40pg的重組蛋白） 。
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