共轉染 的英文怎麼說

中文拼音 [gòngzhuǎnrǎn]
共轉染 英文
cotransfection
  • 共 : 共動詞[書面語]1. （圍繞） surround2. （兩手合圍） span with the hand
  • 轉 : 轉構詞成分。
  • 染 : Ⅰ動詞1 （用染料著色）dye 2 （感染） catch [contract] （a disease） 3 （沾染） acquire （a bad hab...
  1. To investigate the consequence of this interaction, aes - rfp fusion protein expression vector was constructed and co - transfected into nih 3t3 cells with tle1 - gfp fusion protein expression vector. confocal microscopy observation showed that aes could interact with tle1 at the cytomembrane region. moreover, this interaction inhibited the concentration of tle1 into nucleus

    在構建了紅色熒光蛋白aes表達載體后，將其與tle綠色熒尤蛋白載體共轉染細胞，共聚焦顯微鏡觀察發現這兩種分子在胞漿中有共存現象，而且aes的表達可抑制tlei向胞核內的聚積。
  2. The newly molted 5th instar larva of silkworm bombyx mori was infected with the recombinant virus

    另外，通過共轉染和篩選純化獲得了攜帶appa基因的重組家蠶桿狀病毒。
  3. The effectiveness of the nuclear localization signal selected was clearly tested in initial experiments. based on this, the co - transfection systems used in the study was established. we first observed the multinucleate cells and chromatin bridges in cultured hela cells when the cells are marked with gfp and hochest33342, after co - transfection with the gfp expression plasmids and rnai plasmids rhe or rhc

    當分別共轉染附加kozac序列和核定位信號的gfp與人集縮素smc亞基hcap一e特異的rnai質粒rhe和人集縮素smc亞基hca屍一c的f { nai質粒rhc時，都觀察到gfp和hochest33342標記的轉染后hela細胞表現多核和染色質橋現象。
  4. We also conducted the co - transfection with the rnai plasmids rhe and rhc and the selective marker gfp expressing plasmids simultaneously. there are three kinds of combination probability that could be observed by gfp marker

    在實驗中同時共轉染人集縮素smc亞基hcap一e和hcap一c特異的rnai質粒rhe和rhc和pcdna3 . 1 + kg到hela細胞。
  5. They are combination of co - transfection with rhe and gfp expressing plasmids, co - transfection with rhc and gfp expressing plasmids and co - transfection with plasmids rhe and rhc and gfp expressing plasmids at the same time. whichever the combination may be, we always observed the phenomena of chromatin bridge in co - transfected cells. these results indicate that the phenomena of chromatin bridge should be the characteristic feature of deficiency in the human smc subunits

    這時，存在三種可能的情況，即共轉染rhe和pedna3 . 1 * kc 、共轉染rhc和pedna3 . 1 + kg 、共轉染rhe和rhc和pc洲a3 . 1 + kg ，但都觀察到明顯的染色質橋現象，進一步證明這種表型是人集縮素中smc亞基的缺陷的特徵表型。
  6. In this study, the recombinant fowl - poxvirus was transfected into expressing the vp3 gene of isolated gpv h1 strain into the cef cells with fpv - 017 by liposome, which have the lacz reporter gene, earlier / latter promoters lp2ep2 of fpv, promoters p7. 5 and p7. 1 of vaccinia virus, replication unnecessary region of fpv - 017. following 6 cycles screenings, clonings, purification of blue plaques, detection of pcr and dot - elisa, which verified the genetic stable vp3 - fowlpox virus recombinant constructed successfully. this study provided the theoretical and practical foundation for development of gpv recombinant fowl - poxvirus genetic engineering vaccine, as well as provided substance preparatory for prevention the high mortality gpv

    本研究採用脂質體轉染方法，將含有完整gpvh1分離株vp3基因、報告基因lacz 、禽痘病毒早晚期啟動子lp2ep2 、痘苗病毒啟動子p7 . 5 、 p11和fpv - 017復制非必須區的轉移載體質粒psy681vp3lacz與fpv - 017共轉染雞胚成纖維細胞，經6輪蝕斑克隆、篩選、表達， pcr鑒定和dot - elisa檢測，證明該重組病毒已構建成功，並獲得了遺傳性狀穩定的鵝細小病毒vp3基因的重組禽痘病毒。
  7. The pbacph - egf plasmid dna was used to co - transfect bmn cell with the modified bombyx mori baculovirus bm - bacpak dna, which was first linearized by aocl. after two rounds of plaque isolation, the recombinant virus ( named bmbacph - egf ) was further verified with pcr and dot hybridization. the recombinant bmbacph - egf virus was used to infect bmn culture cells at a moi of 10

    重組轉移載體dna與經bsu36i酶切線性化的修飾型病毒bm - bacpakdna共轉染家蠶bmn細胞，兩輪空斑篩選和純化，獲得重組病毒rbmbacph - egf ，經pcr擴增、 dna點雜交等方法鑒定證實重組病毒中已正確插入ph - egf融合基因。
  8. On the other hand, our previous studies have shown that the nr2b construct with a gfp tag in its n - terminus cotransfected with nr1 subunit can reach the cell surface and be detected by surface staining with anti - gfp antibody in live cells. the nr2b construct will be retained in the endoplasmic reticulum ( er ) when expressed alone in heterologous cells

    我們以往的研究表明，用綠熒光蛋白（ gf內在信號膚下游n末端標記nrzb亞單位，與nri共轉染時能形成與野生型相似的nmda受體通道，且可以通過抗gfp抗體標記活細胞膜表面表達的nmda受體復合物。
  9. After transfecting the shrna based on telomerase htert into hela cells with calcium phosphate co - precipitation procedures, we detected hela cells viability by trpan blue staining method

    以磷酸鈣共沉澱轉染法將shrna轉染hela細胞。于不同的時間用臺盼藍染色法檢測細胞生存能力。
  10. The angiostatin baculovirus transfer vector was co - transfected with viral dna into sf9 cells according to the manufacturers protocol. to purify the recombinant virus, we used the plaque assay to screen the pure recombinant plaque and amplify it to generate p - 1 stock

    構建重組病毒：用已經構建好的angiostatin桿狀病毒轉移載體pbluebachiszb和病毒dna共同轉染sfg細胞，通過蝕斑實驗篩選出純的重組斑點並擴增產生p二病毒貯存液。
  11. Finally, a tranfer vector named as pltk - ha was constructed based on pltk - uni with insertion of ha gene of h3 subtype srv. the pltk - ha and prv bartha - k61 genomic dna were co - transfected into 50 - 70 % confluent vero cells in 6 - cm dishes. based on the expression of lacz gene, recombinant prv were selected and purified by blue - colored virus plaque

    利用脂質體介導的方法，將pltk - ha與prvbartha - k61基因組共轉染于亞單層vero細胞，依據報告基因lacz在細胞中的表達，篩選藍色蝕斑的重組病毒，經數次蝕斑純化后， pcr鑒定、 western - blot分析。
  12. After obvious cytopathogenic effects developed, virus - contained supematants were harvested, and the progeny viruses were screened for lacz - expressing viruses by a plaque assay using x - gal. single blue plaques were picked, and a recombinant prv stably expressing lacz gene ( designated as rprv - lacz ) was obtained after ten cycles of plague purification and pcr identification. the results showed that the lacz gene expression cassette was stably expressed in the recombinant rprv - lacz derived from bartha - k61 strain

    該載體與具有高度感染性的bartha - k61株基因組dna通過脂質體加plus法共轉染vero細胞，採用甲基纖維素固定病變， x - gal染色，經過10代藍斑純化獲得了一株穩定表達lacz基因的ge tk基因缺失突變株，命名為rprv - lacz 。
  13. After multiple, plasmid ploxifn and pbs185 dna were co - transfected into the green fluorescent cell clones, then filtrated the non - fluorescent cell clones. two non - fluorescent cell clones, the green fluorescent cell clones and the cells which were co - transfected by the plasmid ploxifn and pbsiss dna were checked up by pcr

    首先將質粒ploxgfpdna電轉染牛胎兒成纖維細胞，並用g418篩選，篩選出綠色熒光細胞克隆，增殖培養之後，將質粒ploxifn和pbs185dna共轉染熒光細胞克隆，篩選出不發光的克隆。
  14. In order to get the soluble recombinant eo protein and inspect the protein expression status convinently, the egfp and eo gene were ligated into baculovirus transfer vector. with the co - transfecting sf9 cells of baculovirus recombinant transfer vector and linearized viral dna, and plaque purification in the posttransfection procedure, the pure recombinant baculovirus were harvested, which infected the sf9 cells for amplifying to generate a p - l stock. in the meantime, the fluorescence microscopy detection indicated expressed egfp protein to confirm the heterogenous protein expression of recombinant baculovirus. the pi - stock from a pure plaque was used to generate a high liter p - 2 stock, which was determined in liter as 1. 14 107pfu / ml by performing a plaque assay. when a volume of p - 2 stock infected the sf9 cells with moi 5 - 10 for expression, the strong fluorescence was obeserved on the day 3 of postinfection

    此外，為了得到可溶性重組eo蛋白並便於觀察重組蛋白的表達情況，我們將egfp基因與eo基因相連插入昆蟲桿狀病毒轉移載體中，與線性桿狀病毒dna共轉染sf9細胞后通過噬斑純化得到純的重組桿狀病毒，將其感染sf9細胞制備p1種子液，同時用熒光顯微鏡觀察綠色熒光蛋白的表達情況剔除表達效果差的重組桿狀病毒。再用p1種子液感染sf9細胞制備高效價的p2種子液。通過病毒液的梯度稀釋和噬斑測定，確定p2種子液的病毒滴度達1 . 14 10 ~ 7pfu ml 。
  15. To check if human rap 1 gap can form dimer in mammalian cells, pcdna - myc - raplgap ( human ) and pmt2 - ha - raplgap ( human ) were constructed and co - transfected into a14 cells

    為解決這個問題，我們構建tpcdna3llmyc rp1gap和pmtz ha raplgap重組質粒，將其共轉染入ai4細胞。
  16. The recombinant pcr technic was used to introduce a linking peptide klgggg to the site between scfv single chain form of the monoclonal antibody sz - 51 specific for the glycoprotein gmp140 on activated platelet membrane and uk32 low molecular weight form of pro - urokinase, to make the scfv - linker - uk32 chimeric gene. this gene was cloned into the transfer vector pbacpak9, and cotransfected with bacpak6 bsu36i digest into sf 9 cells. the fusion protein was secreted into the medium. in the fifth day after the cotransfection, the supernatant of the medium showed 107 iu ml fibrinolytic activity, higher than 25 iu ml fibrinolytic activity of scfv - uk32. elisa showed that the supernatant had the binding activity to activated platelet. wastern blotting also indicated that the supernatant could bind to the monoclonal antibody of urokinase b chain

    為了提高重組導向溶栓分子scfv - uk32的溶纖活性，通過重組pcr方法在編碼scfv與uk32的堿基之間引入編碼klgggg連接肽的堿基序列，並克隆到轉移載體pbacpak9上，通過與線性病毒dna bacpak6 bsu36i digest共轉染到昆蟲細胞sf 9內，進行表達。表達產物分泌到上清中，共轉染后第5d天用纖維平板法測得sf 9細胞上清溶纖活性達到107 iu ml ，比未引入連接肽的scfv - uk32的表達活性25 iu ml高。
  17. Cat ( chloramphenical acetyl transferase ) elisa assays to identified the function of constructed plasmid. the result indicates mutant p1p2, p1p3p2 and p6p2 repressed to cmv promoter. same as ie promoter two special sequences " atcgt " located at two side of the transcription initial site of promoter

    共轉染hplp細胞，結果表明所有的缺失突變體對cmv啟動子都表現抑制活性，我們發現在ie180和p啟動子的轉錄起始位置兩側同時具有兩個特徵序列（ 5 』 atffit 3 』 ） 。
  18. Fusion gene by pcr was inserted into bombyx mori baculovirus transfer vector pbacpak. 8 and contransfected with lineared dna of bm - bacpak6 virus into bmn cells. the homologous recombination occurred inside the cells, and the recombinant virus bacpak - 6aa - hgm - csf was expressed, as identified by pcr and southern hybridization. the bmn cells and the fifth instars were infected by the recombinant virus bacpak - 6aa - hgm - csf

    本研究首先通過pcr將家蠶桿狀病毒多角體蛋白起始密碼子后的18個堿基引入到hgm - csf基因的5 』端之前，然後將融合基因重組與家蠶桿狀病毒轉移載體pbacpak8中，獲得重組轉移載體pbacpak8 - 6aa - hgm - csf ，並與線性化bm - bacpak6dna共轉染家蠶細胞株，獲得重組病毒bacpak - 6aa - hgm - csf 。
  19. Pbluebachisc - vp7 was identified and analped by compnding restriction endonuclease, pcr and nested pcr on the basis of the genetic sites of pbluebachisc, which was idenhfied as vp7 gene of prv hafied pbluebachisc - vp7 and barmidn - blue dna were cbeinfected insect cell sro and harvested mmbinan beculovirus 5d later identification with restriction empme and pcr showed vp7 gene has been arembwt comehy with baculovirus dna and namd a - l

    純化該重組質粒並與線性桿狀病毒dnabac - n - blue共轉染昆蟲細胞sr9 ， 5d后收獲重組病毒。重組桿狀病毒dna分子的pcr及酶切鑒定表明，獲得了prv - vp7基因與桿狀病毒dna的重組子，命名為a - 1代病毒。
  20. Using the green fluorescent cell clones as the template, a product of 4kb which was the same as the result of pcr of the plasmid ploxgfp was got. on the other hand, not only a 4kb product but also a 1000bp product and a 500bp product were showed when the co - transfected cells were used to be the template

    以綠色熒光細胞克隆為模板擴增出了包括gfp基因、 neo基因等在內的約4kb的條帶；以共轉染后的未經篩選的細胞為模板既擴增出了約1000bp的條帶，又擴增出了約4kb和500bp的條帶。
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