噬菌體肽庫 的英文怎麼說

中文拼音 [shìjūntǐtàikù]
噬菌體肽庫 英文
phage peptide library
  • 菌 : 菌名詞1. （蕈） mushroom2. （姓氏） a surname
  • 體 : 體構詞成分。
  • 肽 : 名詞[化學] （有機化合物） peptide
  1. Selection of ligand from a heptapeptide phage display library for lysozyme

    從七肽噬菌體展示庫中篩選蛋白質配基
  2. The monoclonal antibodies ( mab ) specific for the nucleoprotein of prrsv were used to select a phage random heptapeptide library

    採用噬菌體隨機7肽庫對單克隆抗體ge3識別的抗原表位進行了鑒定。
  3. Screening of mimicry peptide of japanese encephalitis virus e protein from phage 15 - mer peptide library

    從噬菌體15肽庫中篩選乙腦病毒糖蛋白模擬肽
  4. The wells of elisa plate were coated with pab ( 100ng / l ) against h3n2, then phage was added to the wells. after incubation, the wells were washed vigorously with tbst to remove nonbinding phage. phage bound to the antibody were eluted with 0. 2mol / l glycine - hcl ( ph2. 2 ) for 10 min at room temperature and neutrialized with 2mol / l tris - hcl ( ph9. 1 )

    以抗h3n2流感病毒的多克隆抗體（ 100ng l ）包被酶標板，加入制備好的肽庫，用tbst洗去非特異結合的噬菌體，加0 . 2mol l甘氨酸-鹽酸（ ph2 . 2 ） ，室溫放置10min以洗脫特異結合的噬菌體， 2mol ltris - hcl （ ph9 . 1 ）中和后，取2 l噬菌體接種大腸桿菌xl1 - blue菌進行空斑滴定，其餘噬菌體擴增後用于下一輪篩選，共重復3輪淘洗。
  5. Phage display describes a selection technique in which a peptide or protein is expressed as a fussion with a coat protein of bacteriophage, resulting in display of the fused protein on the surface of the virion, while the dna encoding the fussion resides within the virion

    自1985年gpsmith首次提出噬菌體展示技術以來，隨著生物技術的發展，噬菌體隨機肽庫已成為研究分子間相互作用的有力工具，特別是在抗原表位研究方面。
  6. In this experiment, to screen the epitope of e2 protein of classical swine fever virus ( csfv ) defined by the monoclonal antibody ( mcab ) all, which had been prepared in previous experiment, the mcab all was raised in mice and followed by purification, the concentration of protein was assayed by using the bca protein assay kit

    本室利用e2基因疫苗制備了多株單抗，為e2的抗原表位研究提供了條件，我們以噬菌體隨機12肽庫分析鑒定了豬瘟病毒e2蛋白的抗原表位，為深入研究豬瘟病毒的抗原結構、制備更有效的診斷試劑和疫苗提供更多理論依據。
  7. By elisa analysis, inhibition of binding of clq with the c ! q receptors on u937 cells and competitive inhibition of binding of clq with aggregated immunoglobulin g b y selected phage clones, and dna sequencing, a number of similar, but not identical, sets of sequences of clq - binding clones were identified. the deduced amino acid sequences of selected 9 peptides are wyegpftlytwp, hwdpfslsayfp, ltqhnspffllp, tsnpfflwypqp, qtpfqlw, npfnwts, spfxlts, fltwldp and fstflyp. they show significant efficiency to inhibit the binding of clq with the clq receptors on u937 cells and / or aggregated immunoglobulin g, which suggest that the selected peptides contain the modeling epitopes of clq receptor to bind the collage - like region or igg to bind the head domain of clq

    然後，採用噬菌體肽庫技術，以c1q為釣餌蛋白，從12肽庫和環7肽庫中親和篩選能與c1q結合的噬菌體克隆，經elisa 、 u937細胞配體結合抑制試驗、 aigg競爭抑制試驗及dna測序，獲得了9個具c1q抑制活性克隆的dna序列，其相應的氨基酸序列為： wyegpftlytwp 、 hwdpfslsayfp 、 ltqhnspffllp 、 tsnpfflwypqp 、 qtpfqlw 、 npfnwts 、 spfxlts 、 fltwldp 、 fstflyp ，它們可能模擬c1qr和或igg的c1q結合表位並抑制c1q的活性。
  8. In the present study, the express library of monoclonal anti - sp18 scfv ( single chain fragment variable ) gene is constructed and selected for further study of sp18 antigen on mammalian fertilization and embryogenesis. total rna were firstly isolated from these growing hybridoma cells which secretes monoclonal anti - sp18 antibodies. after obtained using rpas system, vh and vl genes were used to assemble scfv gene fragment with a linker primer

    應用重組噬菌體抗體庫技術，從分泌小鼠抗牛精子sp18抗體的雜交瘤細胞系中分離總rna ，克隆抗體重鏈和輕鏈可變區基因，加入連接肽引物（ linkerprimer ）組裝成單鏈抗體scfv （ singlechainfragmentvariable ）基因並用rs引物進行擴增， sfi 、 not酶切，回收后與pcantab5e載體相連，轉化e . colitg1宿主菌，構建單鏈抗體文庫。
  9. Methods : a set of oligonucleotide primers were designed and used to amplify the vh and vl gene from anti - hbsag fab antibodies screened from phage antibody library. the products were cloned into puc19 vector and their sequences were analysed. the vh and vl gene fragments were tethered by a peptide linker and a leader sequence coding region, with the leader sequence added at 5 " terminus of each gene ( l - vh - linker - vl ) and designated as l - scfv

    方法以從噬菌體抗體庫中篩選獲得的抗hbsag的fab抗體基因為模板，分別擴增出其輕、重鏈可變區（ v _ l 、 v _ h ）基因，通過重組pcr方法將輕、重鏈可變區基因用連接肽（ gly _ 4ser ） _ 3的編碼序列連接，並引入前導肽編碼序列，構建具有l - v _ h - linker - v _ l結構的單鏈抗體基因。
  10. Construction of murine phage antibody library and selection of n - peptide - binding single - chain antibodies

    小鼠噬菌體抗體庫的構建和n -肽結合單鏈抗體的篩選
  11. In the present paper the application of dna library, phage display random peptide library, phage antibody library, etc. in the research of serodiagnostic reagents and their special values were reviewed

    本文綜述了基因文庫、噬菌體展示肽庫及噬菌體抗體庫技術在血清學診斷試劑研製中的應用及其各自的優點。
  12. In this study we screen the phage - epitope library in attempt to predict the receptor recognition site on vegf, identify peptide able to block the interaction of vegf - kdr and find a potent vaccine against tumor angiogenesis by targeting vegf

    [方法]以兩個具有中和vegf活性的抗- vegf單克隆抗體jh121 、 vg189為目標，分別對噬菌體隨機7肽、 12肽庫進行篩選。
  13. [ results ] all the selected clones gave strong signal in dot bloting and elisa

    [結果]對肽庫進行三輪篩選后，噬菌體克隆具有良好的富集效果。
  14. In this study, a novel strategy to inhibit the activation of the complement system has been developed. to get the purified clq as the target molecule for biopanning, the first step of our purification was to isolate the clq from clr2cls2 by affinity chromatography on igg - sepharose 4b column

    本研究另闢蹊徑，以補體經典激活途徑始動分子c1q為靶標，採用噬菌體肽庫技術，親和篩選能與c1q結合的噬菌體克隆，研製可抑制補體激活的c1q模擬短肽。
  15. Part ii screening of tnfa mimotopes from phage display peptide library : based on the results of screening tnfa binding - peptides, we have tried to use neutral tnfa mcab j1d9 as target to screen tnfa mimotopes from c7c phage display peptide library, which may be another form of antagonist for tnfa, and the mimotopes were identified by sandwich elisa. after 3 rounds of screening, we got 9 phage clones identified as positive clones which can bind with mcab j1d9. we also identified the binding between mimotopes and tnf receptor by competitive elisa, and the results showed strongly binding. the amino acid sequence results shown three different sequences : c - rrpaqsg - c - nkhnrki - c and c - rgmsrki - c

    在對噬菌體環七肽庫進行三輪親和性篩選后，隨機挑選20個噬菌體克隆， elisa鑒定出9個陽性克隆，經dna測序推出三種氨基酸序列： c - rrpaqsg - c 、 c - nkhnrki - c和c - rgmsrki - c ，其中優勢克隆序列為c - rrpaqsg - c ；鑒定結果顯示陽性克隆能夠與tnf受體結合，並且能夠阻斷tnf與受體的結合，提示篩選得到的環七肽克隆展示肽具有tnf的抗原性及與tnf受體結第一軍醫大學顧士學位論文合的特性，為tnfa表位模擬肽。
  16. Our research can be divided into the following four parts : part i screening of tnfa - binding peptide from phage display peptide library : the tnfa binding - peptides were screened from c7c phage display peptide library by using rhtnfa as target protein and identified by sandwich elisa, for exploring whether the binding - peptides can be used as antagonist of tnfa or not

    篩選tnf小分子模擬肽及結合肽對于tnf研究具有重要的意義。本研究包括以下四個方面： 1 、 tnf結合肽的研究：以rhtnf為靶對噬菌體環七肽庫進行篩選，以尋求可拮抗tnf活性的小分子短肽。
  17. The antibody induced by peptide gwyydal abolished vegf - induced huvec growth in dose dependent which specifically express the kdr. moreover, both of the peptide gwyydal and vasavfysalve displaying on phage also inhibited the growth of huvec

    [結論]從噬菌體肽庫中篩選到了與抗體有高親和力的陽性噬菌體克隆，噬菌體表達的7 、 12肽gwyydal 、 vasavfysalve模擬了vegf的抗原表位，引起顯著的抗- vegf抗體反應。
  18. Phage display technique and its application in toxoplasma gondii research

    噬菌體肽庫展示的應用及其最新進展
  19. Radiolabeling of filamentous phage peptide library with 99mtc and its biodistribution in normal mice

    標記絲狀噬菌體肽庫及其在正常小鼠體內的分佈
  20. These phage display peptides may be the tnfa mimotopes. part iii specific c7c tnfa mimotope phage clone as target for screening of tnfa specific binding - peptides from 12mer phage display peptide library : by using the specific c7c tnfa mimotope as target, we developed a novel approach for screening phage peptide library targeting specific phage display peptides. we used c7c tnfa mimotope phage clone lcs - 7 ( c - rrpaqsg - c ) as target to screen 12mer phage peptide library and identify the positive clones by phage elisa

    3 、以特異性噬菌體克隆為靶篩選肽庫的研究? ?一種肽庫篩選新方法的建立：為了深入研究細胞因於與配基（受體或抗體）相互作用的關鍵殘基及其空間構象，在上述研究基礎上，我們以tnfa表位模擬肽克隆為靶篩選噬菌體十二肽庫，找尋與之結合的tnfa結合肽噬菌體克隆；並以此拓寬了噬菌體肽庫的應用范圍。
分享友人
分享到 Line

分享到臉書