噬菌體轉變 的英文怎麼說

中文拼音 [shìjūnzhuǎnbiàn]
噬菌體轉變 英文
bacteriophage conversion
  • : 菌名詞1. (蕈) mushroom2. (姓氏) a surname
  • : 體構詞成分。
  • : 轉構詞成分。
  • 轉變 : change; convert; transform; turn
  1. E. coli xl1 - blue cells were tansformed by psurfpga and phages were rescued by m13ko7 helper phage particles. results showed that the heterodimeric enzyme was expressed as a fusion protein that matures to an active biocatalyst connected to the coat protein of phage fd

    以構建的粒psurfpga化具有琥珀突的大腸桿xl1 - blue ,以輔助m13k07超感染,進行青霉素g酰化酶基因的表達和在表面的展示。
  2. When phz2057 was introduced into s. lividans zx1, it could confer partial resistance to actinophage hau3 on s. lividans zx1

    將phz2057鉛青鏈黴zx1 ,能賦予鉛青鏈黴zx1對hau3的部分抗性。
  3. Culture of mg7 hybridoma cells and detection of antigen - binding affinity of mg7 mab by elisa 2. construction and identification of mg7 recombinant phage antibody library mrna was isolated from cultured mg7 hybridoma cells and converted into cdna ; the variable fragments of heavy and light chain were separately amplified and assembled into scfvs with a specially constructed dna linker by pcr. the scfvs dma was ligated into the phagmid vector pcantabse and the ligated sample was transfered into competent e. co / / tg1 to generate a bacterial form of mg7recombinant phage antibody library ; volume and recombinant ratio of the library were evaluated by means of bacterial colony counts and restriction analysis ( ecor i and hind iii )

    Mg _ 7重組庫的構建及鑒定從培養的mg _ 7雜交瘤細胞中提取並分離mrna ,反錄成cdna ;利用pcr分別擴增mg _ 7單抗的重鏈及輕鏈可區基因,並通過? dna連接子將二者連接起來形成mg _ 7單鏈抗基因;將mg _ 7單鏈抗基因插入pcantab5e ;將連接產物化感受態tg1大腸桿,制備細形式的mg _ 7重組庫;通過落計數和限制性酶切分析( ecor和hind )評估mg _ 7重組庫的容量和重組率。
  4. Thioredoxins, an ubiquitous small proteins with a redox active disulfide bridge in its conserved motif - cp ( g ) pc -, are universally distributed in eucaryote and procaryote and have a molecular mass of approximately 12kda. by its disulfide / dithiol interchange reaction, this protein can transmit the regulatory signals to seleted targets ( enzymes, transcription factors etc ) and plays an important role in many plant physiological processes that includes photosynthesis, dna synthesis, transcription, protein disulfide reduction, protein repair, filamentous phage assembly, cell apoptosis and seeds germinating and so on

    該蛋白質中含有保守的- cp ( g ) pc -氨基酸活性基序,該基序中的兩個半胱氨酸殘基可通過巰基二硫鍵的換實現其氧化還原狀態的化和電子氫的傳遞,對細胞中與氧化還原相關的多種生理過程的調節起重要作用。通過同許多酶類、蛋白類、細胞內活性因子相藕連, trx能對光合作用、 dna復制、基因錄、細胞凋亡和生長、組裝、蛋白質的還原和修復信號傳導等生理過程產生影響和調節。
  5. In the present study, the express library of monoclonal anti - sp18 scfv ( single chain fragment variable ) gene is constructed and selected for further study of sp18 antigen on mammalian fertilization and embryogenesis. total rna were firstly isolated from these growing hybridoma cells which secretes monoclonal anti - sp18 antibodies. after obtained using rpas system, vh and vl genes were used to assemble scfv gene fragment with a linker primer

    應用重組庫技術,從分泌小鼠抗牛精子sp18抗的雜交瘤細胞系中分離總rna ,克隆抗重鏈和輕鏈可區基因,加入連接肽引物( linkerprimer )組裝成單鏈抗scfv ( singlechainfragmentvariable )基因並用rs引物進行擴增, sfi 、 not酶切,回收后與pcantab5e載相連,化e . colitg1宿主,構建單鏈抗文庫。
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