培加酶 的英文怎麼說

中文拼音 [péijiā]
培加酶 英文
pegademase
  • 培 : 動詞1. （在根基部分堆上土） bank up with earth; earth up 2. （有目的地使成長、壯大） cultivate; foster; train
  • 酶 : 名詞[生物化學] （生物體的細胞產生的有機膠狀物質） enzyme; ferment
  1. This experiment passing to grope for the carbon source constitutes of the culture medium and using t. reesei rut c - 30 induced the expression of # - mannanase ( # - 1, 4 - mannan mannohydrolase ec 3. 2. 1. 78 ). in this experiment i put the constant carbon source ( lactose and locust bean gum ) in the foundation culture medium ( mandels nourishment liquid ) of t. reesei rut c - 30, then proceeded the variable carbon source ( dragon spruce fiber, com rush pith fiber, wheat straw fiber, wheat straw xylan, corn rush pith xylan, dragon spruce mannan ) to single factor, double factor, three factor, four factor and five factor orthogonal experiment. 1 determined the activity of p - mannanase using locost bean gum as substract by the 3, 5 - dinitosalicylic acid method, and observed the growing situation of the gernic at the end i selected the directions for the inducement expression of the # ? mannanase from trichoderma reesei rut - c30 that contained the dragon spruce fiber, wheat straw xylan, dragon spruce mannan

    在里氏木霉rutc - 30的基礎培養基（ mandels營養液）中加入固定碳源乳糖和槐豆膠，然後將可變碳源（雲杉纖維、玉米芯纖維、麥桿纖維、麥桿木聚糖、玉米芯木聚糖、雲杉甘露聚糖）進行單因子、雙因子、三因子、四因子、五因子的里氏木霉rutc - 30正交培養實驗，並以槐豆膠為底物用3 ， 5二硝基水楊酸法測定培養液中?甘露聚糖酶的活力。從而確定了酶活最高且菌體生長良好的含雲杉纖維、麥桿木聚糖和雲杉甘露聚糖的誘導培養基為最佳培養基，用該培養基培養的里氏木霉（ t . reesei ） rutc - 30使其轉錄的-甘露聚糖酶（ - 1 ， 4 - mannanmannohydrolaseec3 . 2 . 1 . 78 ） mrna量能夠滿足rt - pcr的要求。
  2. It is the optimal time for subjecting creatine to the medium when cultured to 12h and the concentration of creatine was 0. 75 %. creatine, sarcosine and choline chloride could induce the creatinase production and creatine was the optimal inducer, but creatinine and urea could not induce the creatinase production. 3 purification of creatinase the process of creatinase purification was performed as follows : first the enzyme was completely precipitated in the range of 40 - 80 % of saturation with ammonia sulfate fraction precipitation

    最佳氮源為玉米漿和蛋白腖，最佳比例為2 ： 3 ，最佳濃度為1 . 6 ；加入其它碳源時有助於菌株穩定產酶； 100ml搖瓶的最佳裝液量為15ml ；肌酸、肌氨酸和氯化膽堿都能誘導菌株產酶，其中肌酸誘導產酶的效果最好，而肌酐和尿素不能誘導菌株產酶；誘導物肌酸的最適加入時間為接種培養12小時后，最適加入量為0 . 75 。
  3. 3 warm your reagents ( media, pbs, trypsin ) to 25 - 37 c prior to use

    細胞操作前要把培養液、 pbs和胰酶在37c水浴中加溫。
  4. Methods : hyperosmotic pressure animal model was established by administering 3 % sodium chloride as drinking water to rats or increasing osmotic pressure of the culture medium. osmoregulation positions in the brain, reciprocal projection pathways between the medullary visceral zone ( mvz ) and supraoptic nucleus ( son ) or hypothalamic paraventricular nucleus ( pvn ), oscillation of intracellular calcium in cultured neurons and astrocytes were studied by means of anti - fos, glial fibrillary acidic protein ( gfap ), tyrosine hydroxylase ( th ) or vasopressin ( vp ) multiple imrnunohistochemical staining, immuno - electronic microscope, wga - hrp retrogradely tracing and cell culture methods. results : ( 1 ) fos positive neurons within the mvz, parabrachial nuclei, locus ceruleus, pvn, son, subfomical organ increased markedly

    方法：通過給予大鼠飲用3氯化鈉或提高培養基滲透壓濃度的方法復制高滲刺激模型，主要採用抗fos 、膠質原纖維酸性蛋白（ gfap ）和酪氨酸羥化酶（ th ） （或加壓素? vp ）免疫組織化學多重染色、免疫電鏡、 wga - hrp束路追蹤結合免疫組織化學多重染色、細胞培養等實驗方法，系統觀察了中樞參與滲透壓反射的調控部位、下丘腦視上核（ son ）神經元? ast超微結構的變化、延髓內臟帶（ mvz ）和son及下丘腦室旁核（ pvn ）之間往返投射通路和神經元的性質及其與ast的關系、培養神經元和ast內鈣波的變化。
  5. The xylanase activity of the mutant kept stable after 10 generations. after orthogonal designing experiment, the optimum fermentation conditions of a. niger j 506 were obtained, which is as followed : concentration of the major carbon resource 4 %, ratio between bran and corncob 5 : 5, concentration of glucose 0. 1 %, concentration of ammonium oxalate as supplemental nitrogen resource 2. 0 %, the initial ph of liquid medium 5. 0, 100ml / 250ml flask

    經過正交試驗設計，得出突變株a . nigerj506產木聚糖酶最佳的工藝條件為：主碳源濃度4 、麩皮與玉米芯的比例為5 ： 5 、輔加碳源葡萄糖的濃度是0 . 1 、輔加氮源草酸銨的濃度是2 . 0 ，培養基初始ph為5 . 0 ， 250ml三角瓶的裝液量為100ml 。
  6. Secondly, analysis of peroxidase isoenzyme with polyacrylamidedel electrophoresis for was performed in order to investigate the changes of gene expression under sound stimulation. it could be seen from electrophoresis gel that each group had 6 enzyme bands. new enzyme band in pod electrophoretogram was n ' t detected for stressed groups

    此外，在部分實驗組的培養基中加入不同濃度的蛋白質合成抑制劑環己亞胺酮（ chm ）后發現， pod和cat的活性有所降低，暗示著聲波處理使保護酶活性升高的原因可能是聲波處理促進了細胞內酶的合成。
  7. When sparsely - planted rice overgrew its earing period, light intensity in the mid - lower part increased, photosynthesis prolonged, activity of nitrate and peroxide decreased, protein content increased by more than 50 %, velocity of leaf ageing slowed, but accumulation of dry matter of grain accelerated and 1000 - grain weight increased too

    摘要稀植栽培的水稻，抽穗后田間表現為中下部光強明顯增加，光合作用時間延長，硝酸還原酶和過氧化物酶活性降低，蛋白質含量增加50 %以上，葉片衰老延緩，籽粒干物質積累加速，千粒重提高。
  8. Method four bacteria were separately cultured and measured the transparent circle after adding the lodine solution

    方法將各菌種接于澱粉酶試驗培養基，培養后滴加稀碘溶液，觀察透明圈，判定產酶能力。
  9. The optimum conditions in wheat bran and rice bran on solid state fermentation were 3 % corn starch as additional carbon sources, 4 % ( nh4 ) 2 so4 as additional nitrogen sources, 60 % moisture content of the medium, 25

    在麩皮米糠培養基中，添加3的玉米澱粉、 4的（ nh _ 4 ） _ 2804可顯著提高固體曲中植酸酶酶活，培養基最佳含水量為60 ；最佳產酶溫度為25 。
  10. Effect of yeast cultures on fibrolytic bacterial population and activities of fiber hydrolytic enzymes in the rumen

    添加不同酵母培養物對瘤胃纖維分解菌群和纖維素酶活的影響
  11. In vitro injury models of brain slice ( ogd and nmda insult ) and primary neuronal cultures ( nmda insult ) oxygen / glucose deprivation ( ogd ) - induced injury of rat hippocampal slice in vitro the rat hippocampal slices prepared were allowed to recover in the normal artificial cerebrospinal fluid ( acsf ) bubbled with gas mixture of 95 % o2 + 5 % co2 for 1 h, then they were thansfered to glucose - free nacsf which was bubbled with gas mixture of 95 % n2 + 5 % co2. after treatment with ogd, the slices were placed into 2 % ttc solution in dark and incubated at 37 * cfor 1h. the slices were weighted and a 50 : 50 mixture of ethanol / dimethyl sulfoxide was then added to extract the formazan in dark for 24 h

    離體腦片損傷模型（ ogd和nmda ）及原代培養神經元nmda損傷大鼠離體海馬腦片缺氧缺糖（ ogd ）損傷大鼠離體海馬腦片制備后，在通氧混合氣的正常腦脊液（ nacsf ）中恢復60min ，然後移入通氮混合氣的無糖腦脊液（吵化sf ）中缺氧缺糖，取出腦片與2 ttc避光37 』 c溫浴60dll ，染色后根據每克濕重加入20ml抽提液（乙醇：二甲亞礬一50 ： 50 ） ，在密閉容器內避光置24h ，測量前搖勻后取200pi至96孔板，在490urn波長，酶標儀測定各孔od值。
  12. The techniques used in soft - thick luzhou liquor production, which include the enrichment of microbial in manmade cellar mud, koji making technology, piling, fermentation and distil improvement, are described

    摘要綿柔豐滿濃香型白酒生產中應重視如下四方面的技術措施：人工窖泥培養微生物區系的完善性、大麴培制工藝中增加蛋白源和提高蛋白酶活力、堆積發酵在生產中的應用及蒸餾設備的改進。
  13. A gene encoding choline monooxygenase from atriplex hortansis and the approach to produce plants of salt and drought tolerance. shouyi chen, yiguo shen, jinsong zhang and baoxing du

    膽堿單加氧酶基因及培育耐旱耐鹽植株的方法.陳受宜，沈義國，張勁松，杜保興
  14. L lp - hsdl ma of i. 5kb was detected in the rna extracted from cult ' ured chorionic trophoblast using northem blot hybridhation method. the expression of ll6 - hsdl wa was induced by treating the chorionic cells with dexamethasone ( l0 - ', l0 ' m ) for 24h, and the induction was blocked by co - treatinent of the cells with ru486. these results suggest a gr - mediated effect of glucocorticoid on the rnrna expression and reductase activity of l l 9 - hsd1

    人h合成的肌?一加x門』 m ）對培養的絨毛膜滋養層細胞的11p hsdi還原酶活性具有誘導作用，此誘導作用可以被gr阻斷劑ru486門0川）所阻斷； northern印跡雜交方法進一步證實， dex亦可誘導絨毛膜滋養層細胞11p叫inrna的表達，此誘導作用也可以被ru486門0叫川）所阻斷
  15. All other chemicals were of reagent grade. method : 1. mtt assay : for determining the influence of the medium containing 17 - estrogen or progesterone on cells growth, caov3 were treated with them for 48h and then determined in mtt assay

    2細胞端粒酶制備：將培養的細胞實驗組加人含不同濃度的17p一雌二醇和孕激素的培養液，對照組加同實驗組等量的乙醇門人分別培養12h上4h 8h 。
  16. The results showed : the best additive water to wheat bran solid medium was 35 percent. a. niger an01001 produced high activity phytase when it was cultivated at 30 ? from 90 to 120 hours, and obtained the highest enzyme activity at 114 hours. phytase activity was effected by different buffer and ph, enzyme activity was high at ph 5. 5 and 6

    對該菌的產酶條件進行研究的結果表明：麩皮固體培養基的最適加水量為35 ； 30培養90 120h之間產酶都比較高，在114h酶活最高；在提取時不僅ph對酶得率有影響，而且不同提取液所要求的最適ph也不同，用乙酸緩沖液提取時，當ph為5 . 5 、 6 . 5時酶活較高，進行水提取時，在ph7 . 5時酶活最高；利用cacl _ 2溶液進行提取的濃度達到3時，酶活最高。
  17. Amperometric immunosensors are highly sensitive to multiple electrochemical analytical technology and conventional elisa method. compared other electrochemical sensing technologies, they are the advantage of low cost, simplicity to use, high sensitivity, ease to digitize and real - time output. one of the obstacles retarding the practical application of immunosensors is their reusability

    安培分析電化學免疫傳感器是一種耦聯電化學分析技術和傳統的酶聯免疫技術的高靈敏度傳感裝置，與其它電化學免疫傳感技術相比，更加靈敏、快速，而且裝置便宜，操作簡單，易數字化，能方便地實現實時輸出和監測。
  18. Amperometric immunosensors are highly sensitive to multiple electrochemical analytical technology and conventional elisa method. compared other electrochemical sensing technologies, they are advantage of low cost, simplicity to use, high sensitivity, ease to digitize and real - time output. one of the obstacles retarding the practical application of immunosensors is their reusability

    安培分析電化學免疫傳感器是一種耦聯電化學分析技術和傳統的酶聯免疫技術的高靈敏度傳感裝置，與其它電化學免疫傳感技術相比，更加靈敏、快速，而且裝置便宜，操作簡單，易數字化，能方便地實現實時輸出和監測。
分享友人
分享到 Line

分享到臉書