培隆 的英文怎麼說

中文拼音 [péilōng]
培隆 英文
péron juan
  • 培 : 動詞1. （在根基部分堆上土） bank up with earth; earth up 2. （有目的地使成長、壯大） cultivate; foster; train
  • 隆 : 隆Ⅰ形容詞1 （盛大） grand2 （興盛） prosperous; flourishing; thriving 3 （深厚; 程度深） deep; in...
  1. Bone marrow mesenchymal stem cells of rats for in vitro repair of cortical neurons following anoxia - reoxygenation

    大鼠嗅鞘細胞原代培養克隆樣生長的人為干預效應
  2. In this study, iltv - nm98a strain and iltv - wanggang strain were multiplied in chorioallantois. a pair of primers were devised according to the nucleic acid sequence of iltv tk gene and the dna of multiplied virus was used as pattern to amplify the gene of tk by polymerase chain reaction ( pcr ). the product of pcr was linked with suitable plasmid. then, the recombined plasmid was converted to escherichia coli. the converted escherichia coli

    根據已發表的iltvtk基因的核苷酸序列設計一對pcr引物，以增殖的兩株iltv的dna為模板，分別對它們的tk基因進行pcr擴增。將回收的pcr產物連接到適當的質粒載體上，轉化感受態大腸桿菌，通過篩選對iltvtk基因的陽性克隆進行擴增培養。
  3. Part 1 : the culture and identification of es - d3 cells and the study of the efficiency of eb formation from es cells when grown on mef feeder layer in es culture medium or cultured in es culture medium supplemented with lif 1000u / ml, es - d3 cells being used in our experiments formed normal clones, expressed akp and kept their normal karyotype over many passages. the in vitro and in vivo differentiation experiments showed that es - d3 cells could differentiate into variety of cell types derived from three primary germ layers

    結果顯示： eso3細胞在小鼠胚胎成纖維細胞上和或含白血病抑制因於億f ）的es細胞培養液中形成典型的胚胎幹細胞克隆，堿性磷酸酶染色結果為強陽性，具有正常二倍體核型以及具有在體內外分化為三個胚層來源的組織細胞的潛能，而且具有形成種系嵌合動物的能力。
  4. Many studies show that leafy is high homolog even among distantly related plant species. exception of these, little studies on tissue culture and transformation of ginkgo have been done. this paper emphasizes on the isolation, cloning and analysing two ginkgo orthologs of leafy from the male tree

    為此，本實驗從銀杏leafy同源基因的克隆入手，分析其雌雄株lfy基因結構差異，構建lfy基因的植物正義反義表達載體，建立矮牽牛遺傳轉化體系，以研究銀杏lfy同源基因的功能，同時建立了銀杏組織培養體系，為銀杏的遺傳轉化和提早開花結果奠定基礎。
  5. 2. isolation and cloning of mouse embryonic germ cells of icr species primordial germ cells ( pgc ) were isolation from 8. 5 - 15. 5 dpc ( days post coitum ) embryos. eg cell lines with the characteristic of murine es cell line were established and continuously cultured to 6th passage

    Icr小鼠eg細胞的分離克隆研究本試驗以icr品系小鼠胚胎8 . 5 15 . 5dpcpgc為材料，經傳代培養，獲得能連續而穩定傳至6代的，具有胚胎幹細胞諸多特性的eg細胞系。
  6. These observations show that reprogramming is easier in interspecific embryos reconstructed with es cells than that reconstructed with somatic cells, and that es cells have the higher ability to direct the reconstructed embryo development normally than fibroblast cells. oocytes were reconstructed with outbreeding kunming albino mouse es cells and enucleated rabbit oocytes, and the effects of the passages of es cells and 6 - dmap on the development of interspecific reconstructed oocytes were analyzed. the interspecific reconstructed es - rabbit oocytes were activated either by combined two set electric pulses and 6 - dmap or by two set electric pulses

    以上結果顯示， 6 dmap能增加胚胎幹細胞異種重構卵的卵裂率，對重構卵囊胚的發育率影響不大；高培養代數和低培養代數的胚胎幹細胞用於異種核移植不影響異種重構卵的卵裂率和囊胚發育率；用胚胎幹細胞作為供核細胞；比體細胞作為供核細胞所構建的異種重構胚更容易進行重編程，並且胚胎于細胞指導異種克隆胚胎正常發育的能力強于體細胞。
  7. The deleted mutant pap gene was also cloned into yeast secreted expression ppic9k vector to form ppic9k ~ 3, then the vector was transferred into pachia pastoris gs115 strain. the specific expression protein was secreted into the medium after inducing with methanol and the protein amount reached about 50 - 60 u g per millilitre measured by uv - absorbed methods in the supernatant of the medium via high density fermentation. sds - page results showed that there was one protein band in the gel which molecular weight was about 34ku

    將缺失型pap基因克隆于酵母分泌型表達載體ppicgk構成重組載體，然後導入畢赤酵母（ p8chianastoris ）菌株gslls細胞中，在甲醇的誘導下，經過酵母高密度發酵進行pap的表達，經sds page分析，結果表明，在培養基上清液中含有一明顯的特異性蛋臼條帶，大小為34ku ，經western blotting分析，該蛋白與法國pap抗血清有特異性反應，體外活性檢測表明該蛋白對tmv的侵染性具有高度的抑制性，說明該pap基因在畢赤酵母gs中也得到了正確表達。
  8. Based on the previous studies, the research in this paper was carried out, mainly including two parts as follows : ( 1 ) anammox bacteria and aerobic ammonia oxidizers were detected in situ in 6 sediment samples taken from jiangsu province. molecular techniques, such as fish, pcr, dna cloning and sequencing etc. were used for this purpose. ( 2 ) the continuous cultivation of anammox bacteria from sediment samples were studied, which provides experimental basis for the bioaugamentation of eutrophicated sediment applying anammox process

    本論文在前人研究的基礎上，開展了以下兩個方面的工作： （ 1 ）採用分子生物學技術熒光原位雜交（ fish ） 、多聚酶鏈式反應（ pcr ） 、 dna克隆和測序等對采自江蘇省蘇州市、東太湖、新沂河等6個底質樣品進行了厭氧氨氧化菌和傳統氨氧化菌的原位檢測； （ 2 ）探討了以底質作為接種體進行厭氧氨氧化菌富集培養的可行性，為天然底質環境中厭氧氨氧化過程的強化，富營養化底質微生物修復的可行性提供一定的依據。
  9. Barley yellow dwarf virus often leads to a serious loss in the yield of wheat. but at present, we have not find excellent genes resistant to bydv in wheat, so transferring the excellent genes from relatives to cultivars of wheat will be a good method

    因此，植物耐鹽、耐黃矮病、耐蚜蟲等機理和相應耐性植物的培育，乃至相應耐性相天基因的分離與克隆及其基因工程育種的研究正在受到人們越來越多的關注。
  10. The leymus multicaulis has many excellent traits, such as immune or highly resistant to bydv, and highly tolerant to salt and aphid, which are controlled by some unknown genes

    這些特性都是目前種植的大多數農作物、牧草與草坪草栽培品種，本身缺乏急需改良的特性。因此，分離克隆控制這些優異抗逆特性的功能基因，非常必要。
  11. The results of large - scaled culture show that 48g clone crushed by tissue disintegrator in the course of initial period and its inoculating density is 0. 35g / l, a month later, the clone departed directly and its density is 1. 5g / l, the highest density of each jar could be obtained 410g, the growth speed mostly doubled per week

    大規模培養結果表明在20l廣口瓶中（有效水體15l ） ， 48g克隆在起始培養時經組織搗碎機粉碎切割，切割時間10s （細胞段長度約200um ） ，並按0 . 35g l接種，一個月後不粉碎直接分瓶，分瓶密度1 . 5g l ，一個月後最大密度410g瓶，生長速度約每周翻一番。
  12. The scientist who cloned dolly the sheep hijacked the idea for the experiment from his staff, it was claimed yesterday

    日前，培育出世界首只克隆羊多利的科學家伊恩?維爾莫特被控偷竊了同事的有關克隆羊的構想。
  13. The scientist who cloned dolly the sheep hijacked the idea for the experiment from his staff, it was claimed yesterday. professor ian wilmut, 63, successfully created the world ' s first cloned mammal

    日前，培育出世界首只克隆羊多利的科學家伊恩維爾莫特被控偷竊了同事的有關克隆羊的構想。
  14. Techniques in embryo technology ( such as in vitro production of embryos and animal cloning ) need large quantities of high quality oocytes. but the quality of in vitro matured oocytes from slaughtered animals is generally lower than that of the in vivo matured oocytes. it is usually thought that the reason for this poor quality in in vitro matured oocytes is the lack of capacitation during the dominancy of follicular development in vivo

    目前胚胎工程技術研究和開發（如體外生產胚胎和體細胞克隆等）需要大量高質量的成熟卵母細胞，但利用屠宰動物卵巢卵母細胞經過體外成熟培養而獲取的卵母細胞質量還遠不如體內成熟卵母細胞，其原因一般認為是由於缺乏體內主卵泡階段的獲能作用。
  15. The donkey - adapted strain of eiav ( dv116 ), parental strain of eiav - dla, is a highly virulent isolate which was developed by sequential passaging a virulent eiav strain in donkeys in 1970s. the donkeys inoculated with fatal doses of eiav d strain can always be killed within in the first acute disease period. in this study, we constructed a full - length provirus dna clone of dv116 by pcr

    本實驗中將eiav驢強毒dv體外感染驢白細胞培養物，一定時間后收獲病毒（本文中簡稱dv116 ） ，提取eiav前病毒dna ，以pcr法分別擴增並克隆了包含全長基因的三段前病毒dna片段，以雙脫氧法測定了dv116病毒全基因序列共8236個核苷酸。
  16. In the condition of suspending culture, the gametophytic clone has much higher instantaneous growth rate than stilling culture

    在充氣懸浮培養條件下克隆的瞬時生長率明顯高於靜置培養。
  17. This article mainly discusses the large - scaled cultural conditions of clone in gametophyte laminaria. it studies the proper temperature, light time, light intensity, the consumption of nitrogen and phosphorus, the cultural state and inoculating density on the growth of gametophytic clone, the experiment of large - scaled culture had been done under comparatively proper conditions, in the same time, we studied the growth rule of gametophytic clone in the cultural process

    本文主要探討了海帶配子體克隆的大規模培養條件，對海帶配子體克隆生長的適宜溫度、光照時間、光照強度、營養鹽消耗、培養狀態以及接種密度進行了研究，並在相對適宜條件下進行了大規模培養實驗，同時，對海帶配子體克隆在培養過程中的生長規律進行了研究。
  18. In order to study the function of cycling2 in vitro culturing cell line, we used pires - g2 eukaryotic expression vector transfecting human gastric cell line sgc - 7901 and human embryo kidney hek - 293 cells by lipofectamine plus reagent, and studied the function of cycling2 expression on the cell proliferation in vitro, further investigated the regulation mechanism of cycling2. at the same time, we made a study on the expression level change of cycling2 in normal gastric tissue and different type and different stage of gastric carcinoma tissue. material and method 1 material : piresneo vector was purchased from clonetech, plasmid extraction and purification kit was purchased from qiagen company ; rpmi 1640, dmem fetal calf serum were obtained from gibco / brl ; lipofectamine plus and g418 were purchased from life technologies ; ultrasensitive ? s - p kit, mouse monoclonal antibody p21wafl ( in use ), dab staining kit were purchased from maixin company

    實驗材料與方法1 .實驗試劑高糖dmem 、 rpmll640和胎牛血清購自美國g山eo / brl公司; dmewf12 （ 1 : 1 ）混合培養液購自美國hyclone公司;胰蛋白酶購自美國si目叮a公司; hepes由美國amersco公司分裝;脂質體轉染試劑（ upofectalnineplusreageni ）和以18為美國玩vitrogen公司產品; piresneo載體購自美國cloneteeh公司;質粒提取及純化試劑盒購自德國qiagen公司; ultresensitive翎s一p免疫組織化學試劑盒;鼠單克隆抗體戶3 （ do一7 ）蛋白（即用型） ;鼠單克隆抗體p21waf ， （即用型） ; dab染色試劑盒均購自福建邁新公司;鼠單克隆抗體pziwa曰（濃縮型） ;辣根過氧化酶標記羊抗鼠二抗購自北京中山公司; ecl試劑盒購自美國santacruze公司; dcproteinassay試劑盒購自bi 。
  19. A gene library of psedomonas fluorescens g2 was constructed in the cosmid vector pla2917 using e. coli jm109 as the host strain. two recombinants, pgr3 and pgr7, which can confer glyphosate resistance ofe. coli jm109 were identified from the selective medium containing 10mm glyphosate

    以粘粒pla2917為載體、大腸桿菌jm109為受體菌構建熒光假單胞菌g2的基因組文庫，在含有10mm草甘膦的固體選擇培養基上篩選出兩個耐受克隆pgr3和pgr7 ，插入片段分別為7kb和11kb 。
  20. Sfagm is a five - year project managed by canadas department of agriculture agriculture and agri - food canada and funded by the canadian international development agency cida

    辦公室聯合舉辦的乳品安全控制培訓研討會在和林格爾縣國際大酒店隆重召開。
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