They are jinfo mountain in nanchuan county ( natural protection section ), wuling mountain in qianjiang county ( national emphases forest demonstration county which forest cover rate is beyond 50 % ) and zhongliang mountain in beibei county ( artificial destruction is very grave ). some main land use patterns i. e. woodland, garden, infield, abandon infield, shrub and grassplot are selected in those three sample sites. four aspects on soil fertility index of karst environment under different land use patterns in these three sample sites, are revealed in this paper, by using the field test, indoor measure and analysis, outdoor experiment and field investigation, and the knowledge and technique of soil, ecology, physics and chemistry etc. they are physical characteristic ( effective soil thickness, organic layer thickness, soil texture, water - stable aggregate and soil water etc. ), chemical fertility ( organism, omni - n, omni - p, omni - k, alkali - nitrogen, available p, available k and rapid available k etc. ), soil animalcule ( bacteria, fungi, actinomyces and their grosses ) and soil - seed - pool ( plant community diversity index ) in karst ecosystem
本研究以重慶市的南川金佛山(自然保護區) 、黔江武陵山(國家重點退耕還林示範縣,森林覆蓋率50以上)和北碚中梁山(遭人為破壞嚴重)典型巖溶區為對象,選擇了幾種重要的利用方式,包括林地、果園、耕地、棄耕地和灌草坡,採用野外巖溶生態調查和室內試驗測量分析相結合的方法,以不同土地利用方式巖溶土壤肥力為重點,對不同土地利用方式土壤肥力特徵進行量化分析,找出巖溶土壤肥力差異的主要方面及其根本原因,論文主要從土壤剖面物理退化指標(有效土層厚度、有機質層厚度、質地、團聚體、水分含量等) ,化學肥力退化指標(有機質、全n 、全p 、全k 、
堿解n 、速效p和速效k等) ,樣地土壤微生物指標(細菌、真菌、放線菌數量及總量)以及樣地土壤種子
庫植物群落多樣性等4個方面對重慶典型巖溶區的土壤肥力特徵進行了較為詳細的分析研究,為巖溶地區士壤資源的合理利用及結構的調控管理提供依據。
Two spider silk protein genes ( avfl and avf2 ) screened from the library are characterized in a lot of repetitive motifs, a high guanosines or cytidines content, a strong preference for adenosine or thymidine in the third position of a codon and rich residues of glycines or alanines in the proteins translated
試驗結果,該文
庫容量為4 . 9 10 ~ 6 。從文
庫中篩選到avf1和avf2蛛絲蛋白新基因,具有典型重復序列多, g c含量高,密碼子第三個
堿基偏愛使用a t及編碼蛋白中含有大量gly和ala殘基等特點。
By blasting the homologous sequences in genbank databases, the sequence of grass carp gh cdna from pituitary is 98 % homologous compared with the previously cloned gh cdna of grass carp. the cgh cdna fragment was inserted into pgex - 4t - l to construct the expression plasmid. the recombinant plasmid was digested by bamh i and ecor i to identify whether the cgh cdna fragment was inserted into the plasmid, the pgcgh was transformed into e. coli bl21 competent cells
將得到的序列在genbank和embl數據
庫中進行了同源比較,結果顯示:本研究克隆到的草魚gh基因與genbank中登記的x60474草魚gh基因有12個
堿基的差異,編碼的氨基酸有3個氨基酸殘基的差異,同源性為98 ,影響蛋白質高級結構的保守二硫鍵為2個。
( 5 ) the maximum water level risen by the enclose tideland cultivation around the reservoir is 0. 66m, the responding quality is 2000mvs, which heavily effect the environment around the reservoir. the reservoir " s water level is risen because cross section and flood canal become narrow, which caused by building dam, this makes groundwater unsmooth, drainage, even flow from a lower to a higher space and soil salinized
66m ,且為2000m 』兒的常年洪水,對
庫區周邊環境的影響較大,因打壩築堤縮小過水斷面和行洪通道造成水
庫水位進一步抬升,致使
庫區周邊灌區地下水排水不暢,乃至倒灌,型成士地鹽
堿化。
Inherited genetic variation has a critical but as yet largely uncharacterized role in human disease. here we report a public database of common variation in the human genome : more than one million single nucleotide polymorphisms snps for which accurate and complete genotypes have been obtained in 269 dna samples from four populations, including ten 500 - kilobase regions in which essentially all information about common dna variation has been extracted
我們現在發表一個人類基因組常見遺傳變異的公開資料
庫:這是一個對來自四個群體269個樣品內的一百萬個單核苷酸多態性( snps )進行了完整並準確的分析的研究成果;另外也確認了十個dna片段(每個片段含有五十萬個
堿基)的所有常見遺傳變異資訊。
Qualitative analysis on the effect of flood control and engineering safety -, seizing lots of water conservancy effective adjust storage. then making water level risen and reservoir backwater enlarged in the same flow level ? effect on beach and the buildings across river, soil salinization caused by flow from a lower to a higher place and unsmooth groundwater drainage destroy of travel resource caused by disorder enclose tideland for cultivation in reservoir silt up flood plain are given, then presents enclose tideland for cultivation dyke is important cause which water level rise in reservoir
本文從青銅峽水
庫泥沙淤積入手,分析水
庫泥沙淤積特性和
庫區圍墾堤開發利用淤積灘地的現狀,對防洪安全及工程安全的影響、侵佔大量的有效調蓄
庫容、造成同級流量下水
庫水位抬升水
庫回水上延、對沿岸及過河建築構成影響、因水位抬升致使
庫區周邊灌區地下水排水不暢乃至倒灌而造成土地鹽
堿化、對水
庫庫區大量淤積灘地無序圍墾造成旅遊資源破壞等五方面影響的定性分析,提出
庫區圍墾堤是水
庫水位抬升的重要原因。
The potency of vecuronium is the greatest to adult - nicotinic acetylcholine receptor and the potency of rocuronium is the greatest to embryonic - nicotinic acetylcholien receptor, as rocuronium, vecuronium and atracurium applied individually
對成人型乙酰膽
堿受體抑製程度由大到小依次為維
庫溴銨阿曲
庫銨羅
庫溴銨,其時胎兒型乙酰膽
堿受體抑製程度由大到小依次為羅
庫溴銨維
庫溴銨阿曲
庫銨。
Objective this study was designed to examine the interaction between rocuronium, vecuronium and atracurium on adult - and embryonic - muscular nicotinic acetylcholine receptors with whole cell clamp patch
摘要目的應用全細胞膜片鉗研究羅
庫溴銨、維
庫溴銨或阿曲
庫銨對成人型和胎兒型肌肉乙酰膽
堿受體的作用。
The chloroplast shsp gene was screened from the cdna library of tomato flower by pcr strategy and confirmed by sequencing. but difference was found at 3 bases of the sequence from the reported in genbank. then, an integrated vector prok ii of the chloroplast shsp gene and nptii gene ( a kanamycin resistant gene ) with camv35s promoter was constructed and introduced into tomato mediated by agrobacterium tumefaciens lba4404. transgenic tomato were screened by their ability of growing on media containing kanamycin
本實驗採用pcr方法從番茄花cdna文
庫中克隆到葉綠體shsp基因,經測序證實與genbank中已發表的序列在編碼區相差2個
堿基,其中一個
堿基導致1個氨基酸的改變。將葉綠體shsp基因定向克隆于帶有組成性表達啟動子camv35s的植物表達載體prok中,凍融法轉化農桿菌lba4404 ,利用葉圓盤法對番茄進行ti質粒介導的遺傳轉化。
Chapter 2 screening of genes related to the ovary development of the mitten crab ( eriocheir japonica sinensis ) by cdna macroarray analysis plasmids were extracted from all the clones in the subtracted cdna libraries with alkali lysis method, then the inserted fragments in plasmids were amplified by pcr
2用cdna微陣列篩選中華絨螯蟹卵巢發育相關基因將差減cdna文
庫中的所有克隆用
堿裂解法提取質粒,然後以提取的質粒為模板,用pcr方法擴增插入片段。
The dynamics of soil seed banks and seed movement was investigated in three bare alkali - saline patches in songnen grassland, northeast china, for exploring their potential role in the vegetation restoration of bare alkali - saline patches
摘要在松嫩平原不同退化程度的草地中選取三個次生光
堿斑,通過分析其土壤種子
庫和表面種子流動態,探討了土壤表面種子流在次生光
堿斑植被恢復中的潛在作用。
Abstract : yingdongpo gold mine as an example of gold mine, it ' s oredressing wastewater quality was investigated. alkali chlorinating process being adopted for treating the oredressing wastewater of yingdongpo gold mine, it has achieved good result in removing the cyanide and heavy metal. after analyzing the experimental result for the affecting factors, the project that using the method of intermittence reaction to treat the cyanide wastewater is proposed
文摘:以銀洞坡金礦為例,對選礦廠含氰廢水進行了水質調查,採用
堿性氯化法處理銀洞坡金礦尾礦
庫的含氰廢水,在去除氰化物和重金屬方面都取得了較好的效果,提出了採用間歇式方法處理含氰廢水工藝方案。
In light of great harm from alkali - aggregate to concrete, the alkali activation of the limestone to be used as the aggregate for the concrete in the construction of zhangfeng reservoir is fully tested with petrographic method, accelerated mortar bar method and autoclave expansion test etc., so as to ensure the durability of the anti - abrasion concrete therein
摘要
堿骨料對混凝土的危害很大,為保證抗沖磨混凝土的耐久性採用巖相法、砂漿棒快速法、壓蒸法等4種方法對陽城下川灰巖進行檢驗,以充分揭示張峰水
庫混凝土粗骨料是否存在
堿活性。
Twenty - six induced proteins were analyzed by matrix assisted laser desorption / ionization time - of - flight mass spectrometry ( maldi - tof / ms ) and identified by database searching. among them, 20 ones were assigned and their functions were known, including salt stress proteins, or enzymes are related to salt stress, y - butyrobetaine and iipopolysaccharide synthesis, metabolism pathway and signal transduction
應用maldi - tof ms對26個鹽激誘導蛋白進行分析,將獲得的肽指紋圖譜經數據
庫檢索,初步確定了20個誘導蛋白的功能,其中包括與鹽脅迫、 -丁酰甜菜
堿、脂多糖合成、代謝途徑和信號傳導等有關的酶。
The library consisted of 1. 3 x 106 clones with an average insert size of about 18kb. the capacity of this library was about 20 times the equivalent of the genome of atriplex centralasiatica iljin. screening the genomic library with a 400bp probe located at the 5 ' end of the badh gene obtained by rt - pcr, we got four positive clones
3x10 『個重組噬菌體,插入片段大小約為18kb ,含插入片段的頻率為100隊以中亞濱蓉甜菜
堿醛脫氫酶門adh )基因近5 』端的約400hp片段為探針,篩選中亞濱蓉基因組文
庫,得到了4個陽性克隆。
We analyzed their sequence characterizations, genomic structures and transcription levels under salinity stress. the results indicated that ssvp showed highest homology to the vacuolar h + - ppase ( cvp ) gene from chenopodium rubrum and phylogenetic analysis indicated that ss vp and cvp share a cluster, which showed that they might be more similar in evolution. southern blot analysis showed that there was more than one copy of ssvp in the suaeda salsa genome
本實驗從400mmol lnacl處理的鹽地
堿蓬地上部分構建的zap - cdna文
庫中克隆了編碼mapkk的全長cdna ( ssmapkk , ay093683 )和編碼液泡膜h ~ + - ppase的部分cdna ( ssvp ) ,據已知序列設計引物利用pcr方法獲得編碼鹽地
堿蓬液泡膜h ~ + - ppase的全長cdna 。
Previously, we have constructed a 400mmol / l nacl - treated cdna library of s. salsa and acquired 1000 ests ( expressed sequence tags ) by the large - scale partial sequencing of randomly selected cdna clones. in this research, we isolated a cdna that maybe encode an aquaporin ( s. salsa plasma membrane intrinsic protein, sspip, bf023789 ) located in plasma membrane of s. salsa. then we analyzed the sequence characterizations, genomic structures and transcriptional levels under salinity stress
本實驗室構建了400mmol lnacl處理的鹽地
堿蓬( s . salsa )地上組織的cdna文
庫,隨機挑取單克隆進行測序,獲得了可能編碼鹽地
堿蓬質膜水通道蛋白( s . salsaplasmamembraneintrinsicprotein , sspip )的cdna ,其序列號為bf023789 。
The transgenic arabidopsis plants co - overexpressing of ssnhxi and a vp1 were also been analyzed to examine salt tolerance of the transgenic plants. the results were shown as follows : 1. cdna cloning and sequence analyzing of subunits b, h and c of tonoplast h + - atpase in halophyte suaeda salsa full cdna sequences encoding tonoplast h + - atpase subunits b, h and c were isolated from the cdna library of halophyte suaeda salsa through est analysis
鹽地堿蓬液泡膜h ~ + - atpaseb 、 h 、 c亞基基因克隆及序列分析利用est隨機挑取克隆測序的策略從鹽地堿蓬葉片cdna文庫分離得到編碼鹽地堿蓬液泡膜h ~ + - atpaseb 、 h 、 c亞基的全長cdna序列。