外植體培養 的英文怎麼說

中文拼音 [wàizhítǐpéiyǎng]
外植體培養 英文
explant culture
  • 外 : Ⅰ名詞1 （外面） outside; external side 2 （外國） foreign country 3 （以外） besides; beyond; in ...
  • 植 : Ⅰ動詞1. （栽種） plant; grow; cultivate 2. （樹立） establish; set up Ⅱ名詞（姓氏） a surname
  • 體 : 體構詞成分。
  • 培 : 動詞1. （在根基部分堆上土） bank up with earth; earth up 2. （有目的地使成長、壯大） cultivate; foster; train
  • 養 : Ⅰ動詞1 （供養） support; provide for 2 （飼養; 培植） raise; keep; grow 3 （生育） give birth to ...
  1. In this study, the stem segments of new shoot with axillary buds of well - growth tetraploid black locust trees were used as explants. the effects of different basic mediums, different hormone kinds and their concentrations ratios, different sucrose concentrations on calli induction, buds differentiation and rooting in the process of establishment of high frequency regeneration system of tetraploid black locust were studied. on the base of high frequency regeneration system, the effects of various factors on transformation efficiency of badh mediated by agrobacterium tumefaciens were discussed in the light of gus histochemical assays

    本實驗首先以生長良好的四倍體刺槐優株上當年生新梢的帶腋芽莖段為外植體，研究了在四倍體刺槐高頻再生體系的建立過程中不同基本培養基、不同激素濃度及其配比、不同蔗糖濃度對愈傷組織的誘導、芽的分化及生根的影響；然後在得到高頻再生體系的基礎上，通過農桿菌介導法轉化甜菜堿醛脫氫酶（ badh ）基因，以gus染色組織分析為依據探討了影響轉化效率的各種因素，建立了高效、可重復的基因轉化體系，為四倍體刺槐目的基因的導入打下了基礎。
  2. Explants were then transferred onto the selection medium containing 500mg / l carbenicillin and loomg / l kanamycin and incubated at 25, 16 / 8h light / dark cycle. small leaves of adventitious shoots differentiated from explants were cut and dipped into gus staining solution. positive shoots, gus tinted, were induced to root

    分化出的不定芽切下半張葉片進行gus染色，呈陽性的植株從外植體上切下，在生根培養基（ ms 0刀sing lnaa 50mg幾kan ）中誘導根的形成。
  3. With 24 c or 4 c, the change trends of the content of the salidroside was basically consentaneous in the consecutive cultural eras of the callus. in the callus from the different explants, the influence was maximal to the the activity of pal enzyme and the influence was lowest to the the activity of ca4h enzyme, and the influence to the the activity of tal enzyme was ascertained according to the explant of the callus. so in the same explant with the different temperature or in the different explant with the same temperature, there was no incident between the content of the salidroside and the activity of enzymes pal, ca4h and tal, and we presumed that there may be emphasized particularly on different metabolic pathway of salidroside

    （ 2 ） 、愈傷組織的外植體來源、培養溫度條件和不同的繼代培養數都影響著其中紅景天甙的含量和苯丙氨酸解氨酶（ pal ） 、肉桂酸解氨酶（ ca4h ）和酪氨酸解氨酶（ tal ）這3種酶的活性；不管是葉來源還是莖來源，不管是24培養還是4培養的愈傷組織，在連續繼代培養中紅景天甙含量的變化趨勢基本上是一致的；無論是葉來源的愈傷組織還是莖來源的愈傷組織，培養溫度對pal酶的酶活性影響最大，對ca4h酶的酶活性影響最小，對tal酶的酶活性影響視不同的外植體來源而定；在相同外植體來源的愈傷組織中及不同的培養溫度的條件下，或是在不同外植體來源的愈傷組織中及相同溫度的培養條件下，其紅景天甙含量與pal酶、 ca4h酶和tal酶的酶活性之間沒有完全一致的對應伴隨關系。
  4. Wounded cotyledons of four - day - old seedlings of lettuce ( lactuca saliva l. )

    外植體在無抗生素的誘芽培養基（ ms 0
  5. Floral organs have been successfully induced from the regenerated floral buds of ffyacinthus orienralis l. by precisely controlling exogenous hormones in the medium. under high concentration of cytokinin and auxin, the regenerated floral bud produces only tepals

    以風信子花被片為外植體，通過控制培養基中細胞分裂素和生長素的濃度，可誘導花芽的再生和控制產生花器官的種類，建立起離體花器官定向控制系統。
  6. 2. according to the effect of combination of different hormone concentration in the medium on callus formation and shoot induction of tomato cotvledons, we defined mso + 2. 0mg / l ba + - 0. 2mg / l iaa as optimum differential medium

    根據番茄子葉外植體在加有不同激素濃度配比的培養基上愈傷組織分化和芽再生的情況，確定最佳分化培養基為ms _ 0 + 2 . 0mg / lba + 0 . 2mg / liaa ； 3
  7. Such a piece of transplanted tissue is called an explant, and its placement on the culture medium is called inoculation

    這種被移植的組織稱外植體，將它放置在培養基上稱為接種。
  8. A homolog of ga sbeta - hydroxylase gene expression pattern was examined. hybridization signals of this gene could be detected at day 5 and however, they appear again after day 10 when explants cultured with hormones. it has the similar pattern when the explants cultured without hormones or uncultured

    風信子ga羥基化酶類似基回在外植體表達，在含有外源撒素條件下培養的前5天受到激素的抑制而幾乎不表達，第10天開始在花分生組織中表達。
  9. Application of naa and 6 - ba in tissue culture of sweet potato

    外植體對甘薯莖尖培養與植株再生的影響
  10. Carrot tissue culture and plant regeneration factors including explants, medium and culture condition are combined together to study the most efficient protocol of carrot tissue culture and plant regeneration thereof. the most suitable explant is fresh hypocotyls segment and precultured hypocotyls derived from 7 - 10 day old aseptic plantlets generating in dark or in dim light, the best recipe for cullus induction and subculture is b5c ( 85 with 0. 5mg / l 6ba and 0. 5mg / l 2, 4 - d ), the ideal recipe for plant regeneration is 65 or ms free of hormone. a phytotron with a 16 / 8 h day / night cycle, at 25 is feasible for plant regeneration, and occasional exposure to sun light dramatically stimulates plant growth

    建立了高效的胡蘿卜組織培養及再生體系以適于生產飲料的胡蘿卜「新黑田五寸人參」為材料，研究不同外植體、不同培養基，不同培養條件對胡蘿卜愈傷誘導及再生的影響，建立一套高效的胡蘿卜組織培養再生體系：最適于誘導愈傷的外植體是弱光或黑暗下發芽7 - 10d無菌苗下胚軸，最適合的愈傷誘導培養基和繼代培養是b _ 5c （ b _ 5 + 0 . 5mg l6ba + 0 . 5mg l2 ， 4 - d ） ，最適于植株再生的培養基為不添加任何激素的b _ 5或ms ，組織培養條件為25 、光照周期為16hr 8hr 。
  11. Agobacterium tumefaciens strain a311 carrying the plant tranfer vector pb1121, which contains the neomycin phosphotransferasell gene ( nptll ) and p - glucuronidase reporter gene ( gus ) both under the control of the camv 35s promoter, was used in the establishment of the genetic tranformation of white clover

    選用苗齡4 5天的帶柄子葉作為外植體，先將外植體預培2天，再與根癌農桿菌a311共培養3 4天後，轉入附加有40mg l ~ （ - 1 ）卡那黴素和400mg
  12. The regeneration system of tobacco was established. the adventitious shoots were induced from leaf explants of tobacco based on ms basal medium supple - - mented with 2. 0mg / l 6 - ba and 0. 3mg / l naa. then the regenerated plants were rooted on ms medium containing 0. 3mg / l naa

    植株再生體系的建立採用無菌苗煙草葉片為外植體，以ms為基本培養基，篩選出ms + 6 - ba2 . 0mg l + naa0 . 3mg l以誘導不定芽的分化，並於ms + naa0 . 3mg l的pa碩士學位論文v沉了砂「工隊nk 」主根培養基上生根，獲得完整再生植株。
  13. Leaves of tobacco ( nicotiana tabacum ) were wounded, infected by agrobacterium tumefaciens strain lba4404 and gv3101 : pmp90 harboring expression vector pbgsb and pbglsb and co - cultured for 3 days in darkness on the culture medium ( ms + 0. 5mg / l 6 - ba + 0. 7 % agor ph 5. 7 )

    4以煙草（品種sr ）無菌苗葉片為外植體，採用農桿菌浸染的葉盤轉化法，用構建好的植物表達載體對煙草進行遺傳轉化。外植體置於無抗生素的誘芽培養基（ ms 0
  14. Rate of cluster buds induced by different gingkgo explants is not the same. among them, cluster buds can be obtained directly from stems and mostly from axillar bud, but the amount is little and differentiation rate is only 20 %

    銀杏不同外植體對叢生芽的誘導不同，銀杏莖段培養可以直接得到叢生芽，但數量極少，而且大部分屬于腋芽萌發，分化率20左右。
  15. Pepper ( capsicum annuum l. ) is one of the important vegetables and condiments. in order to laying down the foundation for the selecting and breeding of polyploid of pepper, a experiment of induction polyploids of pepper by colchicines treatment in clones of pepper was carried out

    本實驗用萌動的辣椒種子在ms培養基中培養獲得無菌苗，取下胚軸為外植體，在ms + 6 - ba2 . 0mg l + iaa1 . 0mg l培養基中誘導形成愈傷組織。
  16. Cotyledon and true leaves of stem mustard were cultured on ms medium supplemented with different concentrations of various cytokinins ( ba, cppu, tdz or kt ) alone or in combination with naa or iba, and the adventitious buds regeneration rate was evaluated.

    以榨菜子葉、真葉為外植體進行離體培養，用ba 、 cppu 、 tdz和kt等細胞分裂素和生長素naa 、 iba組合誘導子葉、真葉再生不定芽試驗。
  17. The results are as follows : exogenous hormones enhance hft gene expression. sequence alignment suggests hft ( hyacinthus forkhead transcription factor ) is a novel gene. its expressing level was enhanced by the hormones, and lower level were maintained in explants uncultured or cultured without hormones

    外源激素提高hft基因的表達水平， hft在外植體接種于含有生長素和細胞分裂素的ms培養基上培養24小時、 5天、 10天和15天的材料中均表達，並且其表達水平逐漸增高。
  18. This review includes the following subjects : induction frequency of haploids depend on genotypes, developmental stage of explants, temperature pretreatment of the explants, medium composition and the conditions of cultivation

    迄今己有9個科21種植物用這一方法誘導出單倍體植株，植物的基因型、外植體的發育程度、接種前的預處理、培養基和培養條件等均能影響誘導率的高低。
  19. The transformed hairy roots were detected for agropine to make sure the transformation was successful. a conclusion was reached as follows : agrobacterium rhizogenes r1601 was an ideal strain for hairy roots transformation from trichosanthes bracteata voigt which exhibited extraordinary transformation rate ( 60 % ). od600 = 0. 7 was the optimum infection concentration for the tested transforming agent, with temperature at 25, ph at 6. 0, 20min of infection and 48h of co - culture and phytohormone at 1. 0mg / l being the most favourable hairy root - inducting pattern

    通過實驗和對結果的數據分析，得出下面的結論：發根農桿菌r1601是較好的大苞栝樓發根的誘導菌株，它對預培養24h的大苞栝樓子葉外植體具有最好的轉化效率和發根誘導率（ 60 ） ，最適的菌株感染濃度od _ （ 600 ）為0 . 7 ，溫度27 、 ph值為6 . 0 、 20min的感染時間和48h的共培養，以及黑暗條件和1 . 0mg / l的激素最有利於發根誘導的環境條件。
  20. Aimed at developing a set of methods efficient in inducing the hairy roots with agrobacterium rhizogenes from trichosanthes bracteata voigt to meet needs for hairy roots for bioreactor culture research and application ; and also in order to investigate the roles of explants, agrobacterium rhizogenes, affecting factors and their magnitude and the underlying mechanism in the transformation, therefore to determine the optimum explants, pre - culture time, tested strain concentration, infection time, co - culture time, affecting factors and their effects working together and to obtain the optimum transforming condition by performing orthogonal test, this project was launched. with cotyledons and stems from trichosanthes bracteata voigt plantlets as explants, agrobacterium rhizogenes r1601 and r1000 as infection agents, transformation was performed using different methods and under various circumstances

    為了建立一種有效的誘導大苞栝樓發根的方法及其發根培養體系，以適合於發根生物反應器擴大培養研究和應用基礎的需要；本研究對誘導發根的外植體在發根農桿菌和環境因子等影響作用下進行發根的誘發及其可能的機理進行探討，從而找出用於發根誘導和培養的最佳外植體、預培養時間、發根農桿菌濃度、感染時間和共培養時間、誘導條件，並通過正交試驗得出優化的誘導條件。
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