孵化酶 的英文怎麼說

中文拼音 [fūhuà]
孵化酶 英文
hatching enzyme
  • 孵 : 動詞（使卵內的胚胎發育成雛鳥） brood; hatch; incubate; （of hens, birds） sit on eggs
  • 酶 : 名詞[生物化學] （生物體的細胞產生的有機膠狀物質） enzyme; ferment
  • 孵化 : hatch; incubate; gestation; brooding; hatching; incubation
  1. The hatching enzyme from brine shrimp, artemia shrimp, is a pivotal protease which help the encysted embryo escape from its hatching membrane when hatching

    鹵蟲孵化酶（ he ）是由鹵蟲早期胚胎特異性分泌的、在孵化過程中起關鍵作用的一種蛋白酶。
  2. The he was strongly inhibited by sbti and apmsf, and very sensitive to pmsf, lbti, and tlck, while not sensitive to chymostatin, bestatin, leupeptin, tpck, pepstatin, nem and iam. all these results imply that brine shrimp he was most probably a trypsin - type serine protease. the he could be strongly inhibited by edta in a dose - dependent manner, and 50 mmol / l edta exhibited more than 56. 5 % inhibition

    對孵化酶純化樣品進行生化性質和酶性質分析發現，鹵蟲孵化酶的最適反應溫度約為40 ，最適ph為8 . 5左右；該孵化酶對p - apmsf 、 sbti極為敏感，對pmsf 、 lbti和tlck也非常敏感，但對chymostatin 、 leupeptin 、 pepstatin 、 bestatin 、 tpck 、 nem和iam不敏感，表明該酶極可能是一種屬于胰蛋白酶類型的絲氨酸蛋白酶。
  3. The distribution of the brine shrimp hgcs varies greatly from the species studied till now. one hour after hatching, neither the dorsal - anterior area nor the other dorsal area remained positive immunoreactivity signal. and 2 hours after hatching, there was no typical hgcs in the body of the brine shrimp and the remained hatching enzymes may participate in digesting the left vitellin in the nauplius

    鹵蟲hgc最初出現至孵化前1h時均為全身性分佈，從孵出到孵出后2h ，頭鹵蟲孵化酶的生物化學性質及孵化腺細胞的免疫組織化學研究部的孵化酶顆粒已經減少，而變為非全身性分佈，到孵出后sh ，孵化酶顆粒已基本消失殆盡。
  4. Methods 1 ) statistic methods including factorial experiment was carried out to optimize the major conditions for sample management, and the feasible negative and positive control for fcm analysis of cd62p expression were check out

    方法1採用濃度梯度法優化gprp濃度條件，採用析因設計優化凝血酶濃度和37孵育時間條件，尋找最佳陰、陽性對照。
  5. Protienase assisted hatching in vitro fertilization

    試管嬰兒應用蛋白酶人工輔助孵化
  6. In young chickens aev induces paralysis, ataxia and muscular dystrophy, while in older chickens, infection is usually subclinical, resulting in a decline in egg production and hatchability. infectivity was shown to remain unaffected by chloroform, low ph, pepsin, trypsin and deoxyribonuclease. magnesium cations were shown to stabilise preparations of the virus against heat inactivation. the buoyant density of virions are 1. 31g / ml. the diameter of the virion was estimated to be 22 to 30nm. the aev can be adapted to grow in chicken embryo. the inability of aev to grow effeciently in most cell cultures

    幼雞感染該病毒后，引起麻痹、頭頸震顫甚至共濟失調，而成雞常呈亞臨床感染或導致產蛋量和孵化率下降。病毒的感染性不受氯仿、低ph 、胃蛋白酶、胰酶和脫氧核糖核酸酶的影響，鎂離子可增強病毒對熱的穩定性，病毒的浮密度為1 . 31g ml ，直徑為22 - 30nm ，該病毒主要在雞胚中增殖，在大多數細胞培養物中不生長。
  7. Anti - p21 mouse monoclonal antibody from beijing zhongshan biotechnology anti - mouse or anti - rabbit igg secondary antibody from santa cruz biotechnology ly294002 from sigma biotechnology tritonx - 100 from boehringer mannhein gmbh fluorescein isothiocyanate ( fitc ) conjugated anti - mouse igg antibody was purchased from beijing zhongshan biotechnology hepes from e. metck darmstadt methods superovulation and collection of eggs for superovulation, female kunming mice 4 - 5 week old were injected with pregnant mare serum gonadotropin ( pmsg ), and after 46 - 48 hours with human chorionic ginadotropin. ( hcg ). one - cell fertilized eggs were collected on the next day from oviduct of females

    取4一5周齡成熟雌性昆明系小白鼠，腹腔注射pmsg （孕馬血清促性腺激素） 10iu ， 46一48小時后腹腔注射hcg （人絨毛膜促性腺激素） 1oiu ，將注射hcg后的雌鼠與8周以上的成熟雄鼠合籠交配，次日檢察陰栓，將查到陰栓的雌鼠處死，取輸卵管于mz培養液中，解剖鏡下撕開壺腹，釋放細胞團，然後用300林歲nil透明質酸酶消化去除顆粒細胞，口控吸管將卵細胞在m :中反復清洗，然後置於孵箱中，根據時間點收集g2期細胞。
  8. From the above results it can be concluded that the dorsal - anterior area and the dorsal area are probably the main position of he secretion

    孵出后仍殘留的孵化酶有可能參與了鹵蟲幼體內殘余卵黃顆粒的消化降解，此結論還需進一步驗證。
  9. The brine shrimp he was prepared and purified by 67 % ammonium sulfate precipitation, deae - sepharose fast flow ion - exchange chromatography, and sephacryl column gel - filtration, and its biochemical and enzymological properties were identified in this study. it was found that the deduced molecular weight of he in sds - page is about 98. 5 kda, and its proteolytic activity was optimized at ph of 7. 5 - 8. 5 and at temperature of 40, respectively

    我們利用67硫酸銨沉澱、 deae - sepharosefastflow陰離子交換柱層析和sephacryl凝膠過濾柱層析，並以酪蛋白為其蛋白酶水解活性的檢測底物、以卵膜為其卵殼裂解活性的特異性底物，從鹵蟲胚胎孵化液中分離純化出了鹵蟲的孵化酶分子，其在sds - page電泳中的分子量約為98 . 5kda 。
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