宋內志賀菌 的英文怎麼說
中文拼音 [sòngnèizhìhèjūn]
宋內志賀菌
英文
shigella snnei- 宋 : Ⅰ名詞1 (周朝國名) song a state in the zhou dynasty2 (宋朝) the song dynasty (960 1279)3 (...
- 內 : 名詞1. (內部; 里頭; 里邊) inner; inside; within 2. (妻子或妻子的親屬) one's wife or her relatives 3. (姓氏) a surname
- 志 : Ⅰ名詞1 (志向; 志願) aspiration; ambition; ideal; will 2 (文字記錄) records; annals 3 (記號)...
- 賀 : Ⅰ動 (慶祝; 慶賀) congratulate Ⅱ名詞(姓氏) a surname
- 菌 : 菌名詞1. (蕈) mushroom2. (姓氏) a surname
-
In two of these isolates with typical class 1 integron, it was showed that the integrons were both harbored in plasmids after intll southenbloting both on their genome dna and plasmid dna
1株福氏志賀菌y變種攜帶的基因盒類型與其中一株宋內志賀菌相同,為護漢17和aad減萬。A aada2 cassette, encoding aminoglycoside adenylyltransferase, was found in 2 isolates of shigella sonnei, and a two cassettes array of dfra1, encoding dihydrofolate reductase, and aadala in one isolate of shigella sonnei, and another two cassettes array of dfra1 7 and aada5 in two isolates of shigella sonnei and shigella flexneri y - variant each, and a dfrv cassette in one isolate of shigella flexneri 6, respectively
對其可變區測序分析,其中2株宋內志賀菌i類整合子均攜帶1個基因盒,為氨基糖昔腺營酞基轉移酶( aminoglycosideadenylyltransferase ) az基因( aa朔2 ) ,編碼對鏈黴素的抗性。Among 64 isolates of shigella sonnei and shigella flexneri employed this study, 59 were isolated from sporadic patients, 4 from patients of two outbreak events, and 1 from well water sample involved in one outbreak event, respectively, during 1998 to 2002
本研究的64株宋內和福氏志賀菌中, 63株分別於1998至2002年分離自杭州地區散發腹瀉病人、暴發病人, 1株分離自一起暴發事件的水源。One 3. 4 kb bamh i and pst i restricted fragment with intll positive was cloned from shigella flexneri 2a isolate c101 - 1 and sequenced. sequence analysis showed the fragment with a array of intll, atti site, two cassettes of blaoxa - 30 and aada1, encoding oxacillinase and aminoglycoside adenylyltransferase respectively, and partial sequence of is1, replacing the 3 " conserved region usually found in typical class 1 integron. the gene cassettes regions were examined in all these 30 isolates by pcr with a pair of primers annealing to the sequences of intll and is1, and pcr products with same length were amplif
結果表明,此29株intll陽性志賀菌株有著相同的基因盒區,我們將之稱為志賀菌非典型i類整合子( intll和inth一151同時陽性) ;福氏志賀菌中此非典型i類整合子總浙江大學博士學位論文檢出率高達84 . 8 % ( 28 / 33 ) ,尤其是福氏2a和2b中均為100 % ( 13 / 13和9 / 9 ) :宋內志賀菌中則較低,為3 . 2 % ( l / 31 ) 。6 out of 64 isolates of shigella sonnei and shigella flexneri were positive with all markers of the three region ( 9. 4 % ). all these pcr products of varied regions were sequenced
本文64株福氏和宋內志賀菌株中,三者均陽性浙江大學博士學位論文的菌株僅有6株,陽性率為9 . 4 % 。In this study, we examined class 1 and 2 integron in shigella sonnei and shigella flexneri isolates from hangzhou, china, to investigate their distribution and determine their gene cassette arrays and their location in genome. a atypical class 1 integron without 3 " conserved region was found to prevail among shigella flexneri isolates, and the excision models of it ' s gene cassette were charactered by inverse pcr. moreover the roles of gene cassettes in the atypical class 1 integron and class2 integron to confer resistance were discussed
本研究對杭州地區近年分離的福氏和宋內氏志賀菌株進行類和類整合子的檢測,了解其分佈特徵;進一步通過克隆測序和southenblot ,確定其攜帶的基因盒組成特徵以及其在基因組中的位置;在研究中發現了一種缺少3 』保守區的非典型類整合子,通過反轉pcr了解此整合子的切除基因盒的特徵;並對非典型類整合子和類整合子各自攜帶的aada賦予宿主菌鏈黴素抗性的不同能力,進行了探討。分享友人