寡聚酶 的英文怎麼說
中文拼音 [guǎjù]
寡聚酶
英文
oligomeric enzyme-
In addition to avermectins, s. avermitilis produces oligomycin, a strongly toxic compound. gene deletion vector pxl05 was used to disrupt oligomycin polyketide synthase ( pks ) encoding genes ( olma ) in streptomyces avermitilis cz8 - 73, the producer of anthelmintic avermectins b and the cell growth inhibitor oligomycin. olma gene cluster in the chromosome was displaced by deletion allele on the plasmid via double crossover
本研究以產阿維菌素b和寡黴素的阿維鏈黴菌cz8 - 73為出發菌株，構建了基因缺失載體pxl05 ，並將其轉入cz8 - 73中，通過缺失載體和染色體之間的同源雙交換，對染色體上長達90kb的寡黴素聚酮合酶（ pks ）基因簇（ olma ）進行了缺失。The results showed mn and ni complexes possibly bind to dna by the mode of interaction, whereas zn complex possibly bind to dna by the modes of interaction and electrostatic binding. 5. in addition, we conjugated cleavage system with recognize system and analyzed joint products by hplc, which provide experimental basic for design of dual effects cleavage
此外，本文還選用咖啡酸純品來突破切割體系與識別體系（用氨基臂修飾的寡聚脫氧核苷酸）的連接，並用高效液相色譜法分析其偶聯產物，為今後設計併合成一種具有特異識別和高效切割雙重功能的人工核酸酶提供了實驗基礎。Rapd ( random amplified polymorphic dna ), which bases on the polymerase chain reaction ( pcr ), is by far one of the most commonly molecular techniques to uncover dna sequence polymorphisms. the basic priciple of this technique is that an arbitrary primer ( usually lobp oligonudetide ) is used to amplify random segments of dna, and a small number of fragments will be amplified when the primer anneals on each strand over a length range. if sequence variation is present at the priming site, then a fragment may not be amplied, so the dna polymorphic can be detected
Rapd （隨機擴增多態性dna ）技術是二十世紀90年代發展起來的一項dna分子多態性檢測技術，它建立於聚合酶鏈式反應（ pcr ）技術基礎之上，利用隨機合成的寡聚核苷酸序列為引物（一般為10個bp ） ，分別與dna的兩條單鏈結合，在dna聚合酶的作用下，對基因組的特定區域進行pcr擴增，其電泳結果為不同大小和數目的dna譜帶即rapd圖譜，可反映基因組相應區域的dna多態性。Both of them are localized within the endoplasmic reticulum ( er ) and possess atp - binding sites. envidence has shown that grps function as molecular chaperones by assisting in the proper folding and assembly of proteins within the er
同時他們又是分佈在內質網（ endoplasmicreticulumer ）腔內的分子伴侶，具有弱的atp酶活性，與atp結合后可以協助新生蛋白質的轉位、折疊以及寡聚蛋白的組裝。A reverse - transcriptase polymerase chain reaction ( rt - pcr ) based technique was developed to detect newcastle disease virus ( ndv ) of different poultry species origin. four oligonucleotide primers, based on the differences of nucleotide sequence at the cleavage site of fusion ( f ) protein gene between virulent and non - virulent strains of apmv - 1, were designed to amplify specific dna fragment from different viruses
依據apmv - 1融合蛋白（ f ）基因裂解位點的核苷酸序列與其毒力的相關規律，分別設計合成了四條寡核苷酸引物，建立了一個可迅速檢測不同禽源apmv - 1並可鑒定強、弱毒株的逆轉錄酶?聚合酶鏈式反應（ rt - pcr ）技術。And the two pah ' s of pcr primers that bind to the adapter and the sequence of f fragment close by tn5 respectively were also designed. the genomic dna of b8 was isolated, digested with bamh i, and ligated to the adapter. using the two pairs of the primers, two rounds of pcr were performed hi turn and a fragment of 239bp was amplified successfully. lt was proved by cloning and sequencing that 18bp of the fragment is the sequence opposite to f fragment on the left of tn5 insertion site in b8f, the other is part of the 728 bp of f fragment. this result makes it possible to continue to carry out chromosome walking, to clone and sequence the whole genes of b fragment and f fragment, and to reveal the antagonistic molecular mechanism of b8
試驗研究設計併合成了由40和44個堿基的寡聚脫氧核苷酸組成的染色體爬行接頭，在接頭序列和測定的f片段近tn5的序列上，設計了2對染色體爬行用的pcr引物，從b8菌株中提取基因組dna ， bamhi酶切，與染色體爬行接頭連接，依次用2對引物進行pcr ，擴增出239bp產物，經克隆、測序，發現其中18bp為擴增的相應于f片段在b8f菌株tn5插入位點對面的序列，其餘則為f片段728bp序列的一部分，為進一步進行染色體爬行，克隆和測定整個b和f基因，揭示陽菌株的拮抗分子機制提供了技術資料貯備。分享友人