One 66kd band appeared except 44kd main band when go isozyme above was subjected to sds - page and ce - sds, indicating this go isozyme was similar to that from spinach leaves which contained 40kd and 66kd simultaneously. whether b - mercaptoethanol was added or not when go isozyme was subjected to in sds - page and ce - sds, 40kd main band and 66kd band still appeared, indicating two subunits were not linked by covalent disulfide. amino acid analysis shew that the ratios of basic to acidic amino acid of go isozyme and its 40kd acidic subunit were 0
菜心go同工
酶的sds - page和sds -毛細管電泳( ce - sds )顯示,該
酶除了含40kd主帶外,還有很淺的66kd帶,和之前我們提出的菠菜go同工
酶含40kd酸性亞基和66kd堿性亞基相似; sos - page和ce - sds電泳中,無論加入-
巰基乙醇與否, go同工
酶都只有40kd主帶和66kd淺帶,表明菜心go同工
酶中40kd酸性亞基和66kd堿性亞基不是以共價二硫鍵相連;用制備性sds - page法獲得菜心go同工
酶的40kd亞基,並和菜心go同工
酶一起測定其氨基酸組成,該go同工
酶及40kd亞基的堿酸性氨基酸的比例分別為0 . 66和0 . 54 ,表明40kd亞基可能是個酸性蛋白,而66kd帶則是個堿性蛋白。
There are at least three kinds of ep in petal : thiol - proteinase, metallo - proteinase, serine proteinase. they gather at the site between 43 kd and 66. 2 kd ( about 52 kd ) on the substrate gel electrophoresis. the optimum ph of thiol - proteinase, metallo - proteinase is ph 6, which are looked as acid ep, the serine proteinase has two forms, one is acid ep ( ph 6 ), and the other is alkali ( ph 10 )
花瓣中都至少有3種內肽
酶:
巰基蛋白
酶、金屬蛋白
酶、絲氨酸蛋白
酶,三者聚集在底物膠電泳膠條的43kd 66 . 2kd之間的位置,約為52kd ;前兩種最適ph為6 ,是酸性內肽
酶,后一種有酸( ph6 ) 、堿( ph10 )兩種存在形式,既是酸性內肽
酶又是堿性內肽
酶。
The effects of six effectors, polyethylene glycol ( peg ) 6000, d - sorbitol, trehalose, dithiothreitol, glycerol and glutarie dialdehyd on pal activity in enzymatic reaction were investigated in this article
摘要實驗考察了聚乙二醇6000 、 d -山梨醇、海藻糖、二
巰基蘇糖醇、甘油、戊二醛對
酶反應中苯丙氨酸解氨
酶活力的影響。
These proteases were different with the ones reported in past and it suggests that there may be more proteases associated in psii of chloroplasts of spinach. such as, one of them could be resistant to 1 mol / l nacl and be almost inhibited by 1 mmol / l dtt or mercaptoethanol
比如,一種蛋白
酶對1mol l濃度的nacl溶液不敏感,但是受到dtt和
巰基乙醇的強烈抑制以及1mmol ledta的部分抑制,而且這種蛋白
酶能夠降解18kd外周多肽,在較廣的ph值范圍內都具有活性。
The method was modified by adding suitable insoluble pvp and higher concentration of p - mercapto ethanol in the extract solution. using the modified method, high quality genomic dna prepared from ginkgo, grape and myrica are colorless and are susceptible to digestion with the restriction endonucleases and can be used as template for pcr
我們將ctab法稍做改良:研磨樣品時加入適量的不溶性pvp ,提高-
巰基乙醇在提取液中的比例,從而提取到了無色、可
酶解、可作pcr模板的高質量的銀杏基因組dna 。
By response surface experiment, it was found that the combination use of 0. 54 % peg6000, 0. 62 % trehalose and 0. 31 % dtt could effectively improve the pal activity by 92. 9 %
響應面實驗發現,海藻糖、聚乙二醇6000 、二巰基蘇糖醇在實驗濃度范圍內交互作用很小, 0 . 62 %海藻糖、 0 . 54 %聚乙二醇6000和0 . 31 %巰基蘇糖醇的組合可提高酶活92 . 9 % 。