序列誘導 的英文怎麼說

中文拼音 [xùlièyòudǎo]
序列誘導 英文
sequential induction
  • 列 : Ⅰ動1 （排列） arrange; form a line; line up 2 （安排到某類事物之中） list; enter in a list Ⅱ名詞1...
  • 誘 : 動詞1. （誘導） guide; lead; induce 2. （使用手段引人隨從自己的意願） lure; seduce; entice
  • 導 : 動詞1. （引導） lead; guide 2. （傳導） transmit; conduct 3. （開導） instruct; teach; give guidance to
  • 誘導 : guide; lead; induce; guidance; induction
  1. Objective to induce vancomycin - resistantant s aureus in vitro and to observe the changes of coagulase gene sequences of s. aureus and its relationship with vancomycin resistance

    摘要目的研究萬古黴素敏感金黃色葡萄球菌經過體外誘導變成萬古黴素中介耐藥的金黃色葡萄球菌后凝固酶基因序列有無改變。
  2. In this paper, first strand cdna of 3abc gene was synthesized using template rna extracted from cells infected with fmdv. the complete 3abc gene about isoobp was amplified by pcr and ligated into pgem - t easy vector. after transforming e. coli dh5 a, ampicillin resistant colonies were isolated and plasmid dna was prepared and analyzed by restriction analysis and pcr. presence of the full length 3abc gene was verified by nucleotide sequence analysis and the plasmid containing the expected sequence was named as pgem - 3abc. comparing the aquired sequence of 3abc with that of reference strains, the homology is more than 99 percent. the pgem - 3abc was digested with sal i and bgl ii and ligated into xho i and bgl ii - digested expression vector ptriex - 4 neo. lt was identified by restriction analysis and pcr and sequencing that this fragment had a 17bp deletion hi the nucleotide sequence 708bp of 3abc gene, which happened to form a terminator codon behind 3ab gene, but it contained the complete open reading frame ( orf ) of 3ab gene. positive clones were selected and induced with lmmol / l isopropyl - d - galactoside ( iptg ), bacteria were detected by sds - page and western blotting after properly treated. the results showed that the 3ab gene expressed successfully in e. coli and 33. 5ku fusion protein can be recognized by the positive bovine serum of fmdv. the amount of target protein is over 26 % of the total bacteria protein by gel thin layer scanning analysis

    擴增產物連接到pgem - teasy載體中，轉化大腸桿菌dh5菌株，篩選氨芐青霉素抗性菌落，提取質粒經酶切鑒定、 pcr分析以及確證性測序證明，所克隆的1500bp左右的片段含有完整的3abc基因，與國外參考序列相比，同源性在99以上。將重組質粒pgem - 3abc和表達載體ptriex - 4neo分別用sal和bgl與xho和bgl消化后，亞克隆3abc基因至原核表達載體ptriex - 4neo中，通過酶切鑒定、 pcr擴增以及序列分析，發現克隆到ptriex - 4neo載體上的片段於3abc基因708bp處出現了17bp的缺失，碰巧在3ab基因后形成一終止密碼子，但3ab基因的閱讀框架完整，選出含有3ab基因完整閱讀框架的陽性克隆，用iptg誘導表達，收集菌液進行sds - page電泳、 westernblotting分析，結果表明， 3ab基因在大腸桿菌中成功表達，其表達產物為分子量33 . 5ku的融合蛋白，並能被口蹄疫病毒陽性血清識別。經薄層掃描分析，表達量占總蛋白量的26以上。
  3. Based on the structure and function analysis of hirudin, a potent thrombin inhibitor, and some platelet aggregation inhibitors, which contain the recognition sequence argglyasp as their functional motif, two chimeric antithrombotic molecules were designed by introducing rgd sequence to hirudin cterminus. these chimera genes were constructed by pcr and inserted into the expression vector pet21a, the constructs were confirmed by restriction enzyme digestion and dna sequence analysis. these recombinant plasmids were transformed into

    經限制酶消化和dna序列分析，證明兩種重組質粒與設計完全一致。由於rgd -水蛭素嵌合基因上游連接了金黃色葡萄球菌蛋白a spa的信號肽序列，在iptg誘導下兩種嵌合分子都獲得了分泌表達，表達產物主要集中在細胞周質空間。
  4. Since the important roles of eo protein in the viral infection. immunity and virus - host interaction. the homology of 21 csfv strains was investigated by sequence analysis of eo genes in this study, which will provide some evidence for epidemiological study. in addition, the eo gene of hog cholera lapinized vaccine ( hclv ) strain was expressed in the prokaryotic and eucaryotic systems, and the recombinant proteins were preliminarily analyzed by immunological method

    鑒于eo蛋白在病毒感染，誘導機體免疫及與宿主細胞相互關系中的作用，本研究克隆了2株豬瘟病毒eo基因並將其與其它毒株eo基因進行了序列分析，揭示了我國豬瘟病毒流行株之間的遺傳演化關系，為豬瘟病毒的流行病學研究提供依據。
  5. Bonification and centrifu - gation were recommended for the lysis of collected cells and the supernatant and precipitation were collected respectively. as to the plenty of include bodies in the precipitation, denationalization, detergence, purification and dissolution, last regeneration were recommended to acquire great deal of expressed gst fusion proteins

    Coltblzi生產融合蛋白，重組后的dna序列包括一個pgex質粒，依照pgex質粒的融合蛋白的表達是在tao啟動子的控制之下，而枯啟動子可由乳糖的類似物i剛來誘導它表達。
  6. Above all, this paper discusses the frame, system functions, user demands, construction preconditions and conformity planning based on the introduction of study and application situation ; then, planning project, planning process and foundation of guidance system in grades, including guidance strategy in four grades are studied ; whereafter, the thesis analyzes setting requirements and modes, installation angle, dimension and colour, display contents and arranging sequence and fonts of parking guidance sign, it is mainly studied that calculational method of distance between the variable message signs

    本論文首先在介紹停車誘導系統研究與應用情況的基礎之上，論述系統框架結構、系統功能、用戶需求、建設停車誘導系統的條件與停車誘導系統與智能交通系統的整合規劃等問題;接下來制定了停車誘導系統規劃方案，提出區域性停車誘導系統規劃步驟，研究分級誘導體系的建立方法，提出四級誘導策略;然後分析停車誘導標志布設的具體要求、設置方式、安裝角度、尺寸與顏色、顯示內容及排列順序、字體等問題，重點研究並給出可變信息板設置間距的計算方法。
  7. The experimental procedure that begins with a cloned segment of dna, or a protein sequence, and uses this knowledge to introduce programmed mutations ( through directed mutagenesis ) back into the genome in order to investigate gene and protein function

    在已知dna克隆片段或蛋白質序列上引導程序性的變異（通過直接誘變）並返回到基因組，來研究基因和蛋白質功能的實驗方法。
  8. Rna silencing is a common phenomenon of rna degradation that is induced by homologous sequences. virus and transposon invasions and various kinds of aberrant rnas can provoke rna silencing

    Rna沉默是生物體中普遍存在的一種由同源序列引起的rna降解過程，病毒或轉座子入侵、以及體內產生的各種異常結構rna都可能成為誘導rna沉默發生的因素。
  9. The hwtx - i gene was chemically synthesized according to its known cdna sequence, the gene was inserted into vector ppic9k which contained aoxj promotor and the sequence of a secreting signal peptide - a - factor, the cloning ppic9k / hwtx - i was constructed and confirmed by two - step pcr and dna sequence analysis, then it was transformed into host strain gs115, a his + muts cell line was screened and multicopy transformants were screened by various g418 concentrations, the multicopy transformant was named gh1. gh1 was cultivated in flasks. after 6 days of induction by 0. 5 % methanol, the supernatant was checked by 16. 5 % tricine - sds page, which showed there was a band in the position of 3. 5 - 6. 1kd, then it was isolated and desalted by ultrofiltration followed by ion exchange of cm column, after reverse phase hplc of ci8 and vacuum drying, the purified rhwtx - 1 was obtained which was proved to be correct recombinant hwtx - i by tricine sds - page, maldi - tof mass spectrometry, amino acid composition analysis, the n - terminal amino acid sequence and its biological activity, the final field of the purified rhwtx - i was about 80mg / l, accounting for 23. 6 % of it total secretory proteins

    將帶有hwtx -基因的ppic9k經blgii線性化后，轉化酵母宿主菌gs115原生質體后經篩選陽性克隆並經表型鑒定為his ~ + mut ~ s酵母菌，進一步用遺傳毒素g418篩選多拷貝的轉化菌株，命名為gh1 ；將gh1甲醇酵母菌用0 . 5的甲醇誘導表達，發酵上清經90飽和度的（ nh _ 4 ） _ 2so _ 4沉澱， yw - 3 （ mwc03000 ）的超濾膜超濾，再經cm陽離子交換， c _ （ 18 ）反相hplc純化得到分子量為4kd左右的組分，其中4289 . 05的組分經質譜鑒定，氨基酸組成分析和序列測定為正確的表達產物，生物學活性表明其活性為天然毒素活性70 % ，表達量為80mg / l 。
  10. Analysis on hypervariable sites in mitochondrial dna of malignant transformed cells induced by trans - bpde and nickel sulfide

    致癌物誘導惡變細胞中線粒體高變區序列分析
  11. The high - enzyme activity has 2 base changes, resulting in long amino acid sequence with native amylase. this inducing method resolved the problem of non - effective induction as in base analogue induction. and the method we used provide a new measure for this kind of work

    高酶活編碼區位點突變導致c -端序列變化和終止子的后移本誘變方法克服了用堿基類似物在體內誘變由於核酸復制酶等的校正作用而造成誘變無效的難題，為基因的誘變找到了一條新途經。
  12. In order to study the expression of 3 - defensins in liver as acute phase response proteins, a murine systemic acute phase responsive model was established by intraperitoneal injection o f lipopolysaccharide ( lps ) in our study. the mbd3 cdna sequence ( 145 - 169 bp ) was labled with [ - 32p ] atp as a probe to detect mbd3 mrna in different tissues by northern blot and analyze the time - and dose - dependant expression caused by lps. the 5 " flanking sequence ( - 167 - 179 bp ) of the mbd3 gene was designed as the probe and labled with [ - 32p ] dctp to investigate the binding of transcriptional factors to this region by electrophoretic mobility shift assay ( emsa ) and south - western blot

    以小鼠mbd3基因145 ? 169bpcdna序列合成探針，經[ - ~ （ 32 ） p ] atp標記后通過northernblot方法檢測mbd3在肝臟中的表達，同時分析了mbd3基因誘導表達的組織特異性，劑效和時效關系；結合mbd3基因啟動子區序列分析，以- 164 ? - 179bp雙鏈dna序列合成探針，經[ - ~ （ 32 ） p ] dctp標記后通過電泳遷移率改變實驗（ emsa ）和south ? westernblot方法對參與mbd3在肝臟中誘導表達調節的轉錄因子進行分析。
  13. The results of emsa showed the obvious retardant bands, which suggest that some proteins in nuclei can bind to the specific dna sequence of mbd3 gene after lps treatment. 5. the molecular mass of this dna binding protein was assessed between 43. 0 - 66. 2 kd by south - western blot

    4 .電泳遷移率改變實驗有明顯的滯后帶形成，表明lps刺激后，肝臟組織的細胞核內有轉錄因子與mbd3基因特異的dna序列結合，參與mbd3基因的誘導表達5 . south一westemblot進一步確定此轉錄因子分子量在43 . 0一「 . 2kd之間。
  14. Progress in research on shape memory alloy films in mems field

    磁場誘導有序排列和自組裝的研究進展
  15. In this study, we designed a pair of primers based on the sequence of the upstream and downstream of chicken il - 2 gene. about 600 bp chicken il - 2 cdna fragment was cloned from cona - stimulated chicken splenocytes by reverse transcription - polymerase chain reaction ( rt - pcr ) and was subcloned into puc18 vector. recombinant clone was demonstrated by restriction enzyme digestion and dna sequencing. next, we construct recombinant plasmid pproex ? t - il - 2. the cdna of chicken il - 2 gene was subcloned into bamh i / hind iii sites of vector. the recombinant plasmid pproex ? t - il - 2 was transformed into e. coli dh5a and the bacteria was induced with iptg. it was demonstrated by sds - page and western blot that a 18kda protein which was equal to chicken il - 2 protein in molecular weight was expressed in e. coli dh5a. the expression level was up to 30 % of the total bacterial proteins. the purified protein was used to prepare the antibody against chicken il - 2 protein

    經酶切鑒定及dna序列測定，該基因為雞il - 2基因，其序列與sundick等報道的完全一致。在此基礎上，我們把雞il - 2基因亞克隆到大腸桿菌原核表達載體pproex ~ （ tm ） ht中，構建重組表達質粒並進行確證性序列測定，重組質粒測序結果表明將編碼雞il - 2成熟蛋白的基因正確地插入到原核表達載體pproex ~ （ tm ） ht的目的位點。重組質粒轉化受體菌dh5後用iptg於37進行誘導培養， sds - page和westernblot分析顯示，表達的雞il - 2融合蛋白分子量約為18kda ，表達的融合蛋白經薄層掃描發現目的蛋白表達量約占菌體蛋白的30 。
  16. And many important cis - acting regulatory elements were found in the cloned sequence region by plantcare software analysis. ref promoter was predicted to be most probably induced by light, hot, gibberellin, ethenen or wound

    經plantcare軟體分析發現序列中含有多種調控元件，經預測ref啟動子可能被光、熱、 ga 、乙烯或傷所誘導。
  17. Positive probe was made from the floral meristem materials cultured for 5 days and 10 days with hormones by rna reverse transcription and labelling with radioactive dctp ; two negative probes from uncultured explants and it cultured for 5 days and 10 days. then, we did calculating signal arrays, sequencing, sequence analysis and alignments on genebank etc. finally 229 different ests were got from the cdna library

    用在含有外源激素的培養基上培養5天（花分生組織開始形成）和10天（花分生組織已基本形成）的風信子外植體為材料，構建起cdna文庫。利用cdnamicroarrray技術，經過雜交篩選和est分析序列分析，最終從cdna文庫中獲得了229個不同的外源激素誘導響應的基因序列。
  18. The constructed vector was identified by sequencing. after induction by iptg, four polypeptides coding epitope expressed, respectively. research in this dissertation laid foundation for the development of nd - epitope - vaccine

    經測序鑒定與原序列沒有突變及移碼后，經iptg誘導，有四段含表位的多肽獲得了表達。
  19. By combining the information from the cdna sequences with functions of their homologs, we suggest that two kinds of hormones may play important roles during flower regeneration in a few ways : ( i ) proteolytic system of ubiqutin / 26s proteosome complex, ( ii ) actin - dependent vesicular cycling of auxin efflux carrier, ( iii ) regulating transcription of genes, ( iv ) phosphorylation and dephosphorylation

    經過進一步的篩選，結合序列特徵和同源基因的功能信息對這些基因功能進行了初步分析。認為兩種外源激素可能通過泛素26s蛋白酶體復合體對調節蛋白的降解機制，生長素運輸載體肌動蛋白依賴的小泡運輸，基因轉錄調節，磷酸化和去磷酸化等調節過程誘導風信子花分生組織的再生。
  20. Escs can be induced into neuron - like cells by sequential neural induction the undifferentiated escs grew in colonies, with clear bound. alkaline phosphatase staining showed dark brown positive particles were distributed in every esc colony. after the sequential neural induction, the cells converted into neuron - like cells, with homogeneous forms, which had round bright cell bodies and thin long bipolar or multipolar processes

    5誘導分化前後細胞端粒酶活性檢測（ trap ）結果1 .使用序貫誘導法可將escs大比例誘導分化為神經元樣細胞未分化的escs聚集成團狀生長，細胞排列緊密，集落邊界清楚，堿性磷酸酶染色呈強陽性，胞漿布滿棕黑色顆粒。
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