志賀子 的英文怎麼說
中文拼音 [zhìhèzi]
志賀子
英文
shigako-
Study on molecular epidemiology of shigella in hangzhou district during 1998 to
2005年志賀菌分子分型及變異研究Lately several investigations reported class 1 and 2 integron in shigella strains and their association to antibiotic resistance
新近數起研究報道了志賀菌中類和類整合子與菌株耐菌性關系。A aada2 cassette, encoding aminoglycoside adenylyltransferase, was found in 2 isolates of shigella sonnei, and a two cassettes array of dfra1, encoding dihydrofolate reductase, and aadala in one isolate of shigella sonnei, and another two cassettes array of dfra1 7 and aada5 in two isolates of shigella sonnei and shigella flexneri y - variant each, and a dfrv cassette in one isolate of shigella flexneri 6, respectively
對其可變區測序分析，其中2株宋內志賀菌i類整合子均攜帶1個基因盒，為氨基糖昔腺營酞基轉移酶（ aminoglycosideadenylyltransferase ） az基因（ aa朔2 ） ，編碼對鏈黴素的抗性。" of course i ' ve got a bit of flak from my mates calling me skippy, and the guys from work sent me a get well card that ' s a warning sign for a kangaroo.
當然，我也遭到了妻子的些許抱怨，她管我叫跳不停。公司的同事們送給我一張賀卡，祝福我早日康復，那上面畫了一個袋鼠警示標志」 。One 3. 4 kb bamh i and pst i restricted fragment with intll positive was cloned from shigella flexneri 2a isolate c101 - 1 and sequenced. sequence analysis showed the fragment with a array of intll, atti site, two cassettes of blaoxa - 30 and aada1, encoding oxacillinase and aminoglycoside adenylyltransferase respectively, and partial sequence of is1, replacing the 3 " conserved region usually found in typical class 1 integron. the gene cassettes regions were examined in all these 30 isolates by pcr with a pair of primers annealing to the sequences of intll and is1, and pcr products with same length were amplif
結果表明，此29株intll陽性志賀菌株有著相同的基因盒區，我們將之稱為志賀菌非典型i類整合子（ intll和inth一151同時陽性） ;福氏志賀菌中此非典型i類整合子總浙江大學博士學位論文檢出率高達84 . 8 % （ 28 / 33 ） ，尤其是福氏2a和2b中均為100 % （ 13 / 13和9 / 9 ） :宋內志賀菌中則較低，為3 . 2 % （ l / 31 ） 。In this study, we examined class 1 and 2 integron in shigella sonnei and shigella flexneri isolates from hangzhou, china, to investigate their distribution and determine their gene cassette arrays and their location in genome. a atypical class 1 integron without 3 " conserved region was found to prevail among shigella flexneri isolates, and the excision models of it ' s gene cassette were charactered by inverse pcr. moreover the roles of gene cassettes in the atypical class 1 integron and class2 integron to confer resistance were discussed
本研究對杭州地區近年分離的福氏和宋內氏志賀菌株進行類和類整合子的檢測，了解其分佈特徵；進一步通過克隆測序和southenblot ，確定其攜帶的基因盒組成特徵以及其在基因組中的位置；在研究中發現了一種缺少3 』保守區的非典型類整合子，通過反轉pcr了解此整合子的切除基因盒的特徵；並對非典型類整合子和類整合子各自攜帶的aada賦予宿主菌鏈黴素抗性的不同能力，進行了探討。2. characteristic of atypical class 1 integron during the detection of typical class 1 integron using pcr, we found that among 30 isolates, most of which belonged to shigella flexneri, all were positive for intll but negative for their varied regions, and most of them were also negative for 3 " conserved region except three isolates positive
2 .非典型i類整合子的分佈及一級結構特徵在檢測典型i類整合子過程中，發現了30株intll陽性，但可變區陰性、 3 』保守區陽性僅有3株（陽性率為5 . 1 % ） ，推測福氏志賀菌株中可能廣泛存在著某種非典型i類整合子。分享友人