感桿分體 的英文怎麼說
中文拼音 [gǎngǎnfēntǐ]
感桿分體
英文
rhabdomere-
Newcastle disease virus ( ndv ) strain 695, a thermostable nature avirulent strain, were replicated in embryonated chicken eggsand its rna was extracted from allantoic fluid. referred to the reported sequence of f gene, a pair of primers were designed and synthesized. f gene of ndv b95 strain was amplified by rt - pcr, the pcr products were checked by agrose gel electrophoresis and purified by agrose gel fracion method
利用從國外引進的新城疫熱穩定性天然弱毒b _ （ 95 ）株接種spf雞胚繁殖病毒，經處理后提取病毒的基因組rna ，參考國內外發表的ndv融合蛋白基因序列，設計一對特異性引物，經反轉錄聚合酶鏈式反應（ rt - pcr ）擴增出約1700bp大小的特異性片段，將此片段回收純化后，利用t - a克隆技術將其克隆到pgem - t - easy克隆載體中，再轉化大腸桿菌jm109感受態細胞，轉化后經分子量比較、 pcr鑒定和酶切分析篩選陽性克隆。In this study, iltv - nm98a strain and iltv - wanggang strain were multiplied in chorioallantois. a pair of primers were devised according to the nucleic acid sequence of iltv tk gene and the dna of multiplied virus was used as pattern to amplify the gene of tk by polymerase chain reaction ( pcr ). the product of pcr was linked with suitable plasmid. then, the recombined plasmid was converted to escherichia coli. the converted escherichia coli
根據已發表的iltvtk基因的核苷酸序列設計一對pcr引物，以增殖的兩株iltv的dna為模板，分別對它們的tk基因進行pcr擴增。將回收的pcr產物連接到適當的質粒載體上，轉化感受態大腸桿菌，通過篩選對iltvtk基因的陽性克隆進行擴增培養。The result showed that the homology rate of pila gene among the 5 avian pathogenic e. coli strains tested and one human e. coli were from 89. 8 % to 91. 1 %, and the homology rate of amino acid were from 88. 5 % to 91. 8 %. the homology rate of pila gene sequence among 5 avian pathogenic e. coli strains tested and avian pathogenic e. coli reported ( serotype o1, o2, o78 ) were from 87. 8 % to 90. 2 %, and the homology rate of amino acid were from 84. 6 % to 91. 2 %. there had homology in avian pathogenic e. coli. there had some common antigen side in type 1 pili of avian pathogenic e. coli
結果表明：運用msha法檢測1型菌毛的檢出率為80 （ 36 45 ） ， pcr法的檢出率為95 . 5 （ 43 45 ） ， pcr方法用於1型菌毛的檢測比msha更加敏感、快速、特異性強；選擇5株優勢血清型雞源致病性大腸桿菌代表株（ o _ （ 89 ） ， o _ （ 119 ） ， o _ （ 141 ） ， o _ （ 127 ） ）的1型菌毛pila基因的pcr擴增片段經純化后，分別定向克隆到puc18質粒的多克隆位點，構建了含有目的基因片段的克隆質粒，並轉化到dh5株大腸桿菌載體菌中，篩選獲得陽性克隆菌株。Adventitious shoots could be rooted and formed regenerated plants on 1 / 2 ms medium supplemented with naa0. 4mg / l. hairy roots could be induced from the basal surface of the explants 8 days after inoculation of a. ficoidea cv. " ruliginosa " leaf explants with a. rhizogenes atcc 15834
紅龍草葉片外植體被發根農桿菌atcc15834感染8d后，從形態學下端葉脈處陸續分化出乳白色的不定根， 21d后，葉片外植體的生根率高達92 . 5 。Culture of mg7 hybridoma cells and detection of antigen - binding affinity of mg7 mab by elisa 2. construction and identification of mg7 recombinant phage antibody library mrna was isolated from cultured mg7 hybridoma cells and converted into cdna ; the variable fragments of heavy and light chain were separately amplified and assembled into scfvs with a specially constructed dna linker by pcr. the scfvs dma was ligated into the phagmid vector pcantabse and the ligated sample was transfered into competent e. co / / tg1 to generate a bacterial form of mg7recombinant phage antibody library ; volume and recombinant ratio of the library were evaluated by means of bacterial colony counts and restriction analysis ( ecor i and hind iii )
Mg _ 7重組噬菌體抗體庫的構建及鑒定從培養的mg _ 7雜交瘤細胞中提取並分離mrna ，反轉錄成cdna ；利用pcr分別擴增mg _ 7單抗的重鏈及輕鏈可變區基因，並通過? dna連接子將二者連接起來形成mg _ 7單鏈抗體基因；將mg _ 7單鏈抗體基因插入pcantab5e ；將連接產物轉化感受態tg1大腸桿菌，制備細菌形式的mg _ 7重組噬菌體抗體庫；通過菌落計數和限制性酶切分析（ ecor和hind ）評估mg _ 7重組噬菌體抗體庫的容量和重組率。Meq protein, highly expressed in the insect cell line sf9 by the baculovirus vector was immunized into balb / c mice and the immunized spleen cells were collected and fused with the tumor cell line sp2 / 0 via peg - 1000 in vitro. the hybridoma cells were cloned and screened for the ability of anti - meq mcab secretion by fa with the mdv ga infected chicken embryo fibroblast ( cef )
利用通過桿狀病毒載體在昆蟲細胞系sfg上高度表達的meq蛋白產物免疫balb / c小鼠，然後收獲其免疫脾細胞並與腫瘤細胞系spz / 0通過peg于體外融合;獲得的雜交瘤細胞被克隆並通過與mdv感染的雞胚成纖維細胞（ cef ）做免疫熒光試驗（ fa ） ，進行其分泌抗meq單克隆抗體（ mcab ）能力的篩選。Our company is specialized in supplying all kinds of terminal block, switch test double block, ground block, fuse block, three - level sensor block with light, double block, universal block, integral block, nylon cable ties, cable ties, releasable cablle ties, self - locking cable tie, marker calbe ties, flexible pipe, cable marker, expansion nail / plug, wire joint, cable clamp, nail clip, screw on wire connector, self - adhesive tie mount, spiral wrapping band, wire connectors, cable tie mounts, nylon fasteners, cable gland, nylon ties, cable terminal, terminals, terminal connector, wiring duct, pre - insulated terminals, cable markers, electrician tools, cabling accessories, plastic cable ties, wire tie, tie wrap, beaded tie wraps, tie wrap adhesive, tie wrap tool, cable, wires, terminal connector, terminal block, nail, nylon cable glands, pe tubing and quick screw connector, circle nail cable clips, self - adhesive tie mounts, expand nails, spiral wrapping bands, screw on wire connectors, copper connecting terminals, pre - insulated electrician tools, wire connectors, cable tie mounts, nylon fasteners, nylon ties, cable terminal, pre - insulated terminals, cabling accessories, plastic cable ties, wire tie, tie wrap, beaded tie wraps, tie wrap adhesive, tie wrap tool, cable, wires, terminal connector, terminal block, nail, nylon cable glands, cable accessories, wiring accessories etc. we offer superior product and best service with competitive price
我們公司是專門供應各種終端塊，交換機測試雙重座，地面座，保險絲座，三層傳感器座輕型雙座通用塊，積分塊，尼龍電纜聯系，電纜聯系，松解錨索關系自鎖式電纜接頭，光纜標記關系，軟管，電纜標記，膨脹釘/插頭，電線接頭，電纜鉗，夾釘，螺絲電線連接，自粘配合摩，螺旋帶包，導線連接器，線纜配合固定座，尼龍緊固件，電纜壓蓋，尼龍關系，電纜終端，終端，終端連接器，電線導管，預終端絕緣，電纜料，電工工具，電纜配件，塑料電纜聯系，配合鋼絲，為配合劇情，串珠配合纏上搭膠包裹，包裹配合工具，電纜，電線，終端連接器，終端塊，釘，尼龍索腺體，聚乙烯管材及快速接頭螺絲，圓釘電纜夾，自粘配合固定座，擴大釘，螺旋帶包螺桿導線連接器，銅接線端子，預絕緣電工工具，電線接頭，電纜接頭固定座，尼龍拉鏈，尼龍關系，電纜終端，預終端絕緣，電纜配件，塑料電纜聯系，配合鋼絲，為配合劇情，配合串珠包，搭包膠，配合工具包，電纜，電線，終端連接器，終端塊，釘，尼龍腺體電纜，電纜配件，線路配件等，我們提供卓越的產品和最好的服務，具有競爭力的價格In chapter 2, author points out firstly that the elastic deformation of elastic units of a robot ' s wrist force sensor will be enlarged by the end - effector, the instruments and the work pieces, so the elastic deformation of the sensor will influence the location accuracy or kinetic accuracy of end point of a robot, under the condition of that the robot technology facing the developing of heavy load, light mass and high accuracy. it is discussed respectively that the relationship between the differential kinemics in the sensor ' s coordinate and the location accuracy or kinetic accuracy of the end point. error matrixes of location and kinemics of the end point are presented respectively based on the differential kinemics in the sensor ' s coordinate, and the on - line error compensation methods are introduced subsequently
第二章首先指出機器人腕力傳感器彈性體的彈性變形經過機器人末端連桿、工具、工件等的放大后，會對機器人末端精確定位和運動產生的影響；然後分別研究了傳感器坐標系內的微分運動與機器人末端工件精確定位、運動的關系；在此基礎上，研究了基於腕力傳感器彈性體微分運動的機器人末端定位、運動誤差的誤差矩陣及其在線誤差補償方法；基於機器人動力學的機器人末端定位、運動誤差的誤差矩陣及其在線誤差補償方法；最後，以puma型機器人為對象，給出了基於腕力傳感器內微分運動的機器人末端定位、運動誤差及其在線補償方法的模擬實例：給出了基於機器人動力學的機器人末端定位、運動誤差及其在線補償方法的模擬實例；模擬結果表明， 1 ）基於腕力傳感器的機器人末端定位誤差在腕力傳感器允許的載荷下可達十分之幾毫米級。The transformed hairy roots were detected for agropine to make sure the transformation was successful. a conclusion was reached as follows : agrobacterium rhizogenes r1601 was an ideal strain for hairy roots transformation from trichosanthes bracteata voigt which exhibited extraordinary transformation rate ( 60 % ). od600 = 0. 7 was the optimum infection concentration for the tested transforming agent, with temperature at 25, ph at 6. 0, 20min of infection and 48h of co - culture and phytohormone at 1. 0mg / l being the most favourable hairy root - inducting pattern
通過實驗和對結果的數據分析，得出下面的結論：發根農桿菌r1601是較好的大苞栝樓發根的誘導菌株，它對預培養24h的大苞栝樓子葉外植體具有最好的轉化效率和發根誘導率（ 60 ） ，最適的菌株感染濃度od _ （ 600 ）為0 . 7 ，溫度27 、 ph值為6 . 0 、 20min的感染時間和48h的共培養，以及黑暗條件和1 . 0mg / l的激素最有利於發根誘導的環境條件。This paper gives a comprehensive presentation about the fundamental of the flow - measuring method by flume, the design of khafagi flume and the buoy - level mechanism, the signal conversion by the angle measuring sensor and the deduce of the flow equation. it also introduces the hardware design, the development and emulation on software of the intelligent measuring meter in detail. the error of flow measurement is analyzed and calculated in the end either
本文對槽式流量測量的基本原理、卡發基槽和浮桿測量機構的設計、角度傳感器的信號轉換以及流量計算公式的推導做了全面的闡述；詳細介紹了智能儀表應用系統的硬體電路設計和應用軟體的開發及模擬調試；最後對測量系統的各項誤差進行了分析和計算。The genotype is a main factor in the genetic transformation via agrobacteriwn - mediated. the results of orthogonal experiments of the factors which affect mainly the transformation illuminates that the erniuxin genotype + bacterium 15 x + explant + dip - dye is the best one. the cocultivation time is 28 days. the experiment shows that : the erniuxin cotyledon regeneration frequency is very high, and that the dongnong901 hypocotyl regeneration frequency is very high
確立了農桿菌介導轉化過程中基因型是影響轉化率的主要因素。通過極差分析，基因型為「二牛心」 ，菌液濃度15倍的，外植體為子葉，感染方法為浸泡法是最佳的組合。農桿菌與外植體共培養的時間為28h 。Culture supernatants of bt strains induced luminescence in v. harveyi reporter strain bb170, which can only response to ai - 2 signal moleculars, indicating that the bt strains have the luxs gene. by aligning the nucleotide sequences of the b. anthracis and b. cereus luxs genes, a pair of primers were designed and an 474 - bp fragment was amplified from bt strains by pcr
首先通過生物發光實驗檢測到蘇雲金芽孢桿菌（ bacillusthuringiensis ， bt ）的培養液可以誘導特異性檢測ai - 2信號分子的哈氏弧菌報告菌株bb170發光，表明bt中含有群體感應信號分子ai - 2的合成基因luxs 。With the deep insifht about china market and excellent leverage with its global agents, yicheng logistics can provide tailor made solution to its clients
通過這種代理網路和其對市場的深刻洞察所形成的卓越的杠桿作用，怡誠物流使其每一個客戶充分體會到量體裁衣的感受。The amplified phage be used repeat the selection. the selection was repeat three times. the titter of phage indicated that the eluted phage enriched with the rounds increasing
山西醫科大學碩士學位論文第三次淘洗的噬菌體感染大腸桿菌xli一blue ，鋪制平板，隨機挑取18個分離良好的噬斑，制備噬菌體原種。During light adaptat ion the arrangement of microvillus in the rhabdom was in disorder, and the diameter of rhabdom was reduced. at the same adaptation the area of the perirhabdomal vacuole was reduced and the number of the multi vesicular body was increased. besides that the d i str i but i on and the amount of the p i gment granule, the lamellar body were influenced by light adaptation
光適應時感桿束的微纖毛的平均直徑膨大，排列零亂，感桿束的平均直徑減小；膜下儲泡囊平均直徑減小；小網膜的細胞質中胞飲泡和小囊泡數量較多；多囊體的平均直徑減小，數量較少；板膜體較少，色素顆粒多，分佈於細胞的各個層面。6. transformation system of mustard a serials of kanamycin concentration was added to optimum medium to test the explants resistance capacity of two kinds of mustard. the transformation procedures described were derived from numerous regeneration and trasformation designed to test factors that might affect shoot regeneration, which including length of co - cultivation. those producing the best result parameters were described as below : after the mustard explants were precultured on regeneration medium for 2 days. they were inoculated with agrobacterium for 20 minutes. inoculated explants were co - cultivated for 4 days and in shadow at first 2 days. then transferred to the same medium plus 30 mg / l kanamycin and 500mg / l garb. all of them were transferred to fresh medium every 2 weeks. the kan - resistant plants were regenerated
芥菜外植體高頻遺傳轉化體系的建立在最適培養基上試驗了兩類芥菜的三種外植體對卡那黴素的敏感性、預培養天數、浸菌時間等因素的影響，建立了芥菜高頻轉基因再生體系：取生長4天的芥菜子葉、下胚軸和25天的葉片在分化培養基上（ ms + ba3 . 0mg / l + naa0 . 1mg / l ）預培養2 - 3天後，投入農桿菌菌液中浸染20分鐘，在分化培養基上暗培養2天，正常條件下培養2天後，轉入抗性培養基（ ms ba3These include cell growth characteristics, expression levels, intracellular and extracellular expression, posttranslational modifications, and biological activity of the protein of interest, as well as regulatory issues in the production of therapeutic proteins. in addition, the selection of a particular expression system requires a cost breakdown in terms of process, design, and other economic considerations. section i : construction of pet22b ( + ) / hpk - 5 vector the hpk - 5 gene encoding 82 amino acid residues from c462 to c543 was recombined with the sequence of plasmid pet22b ( + ) for constructed a new expressed vector pet / hpk - 5
方法在對hpk - 5 （ humanplasminogenkringle5 ， hpk - 5 ）因子的基因序列和蛋白質序列進行分析的基礎上，利用pcr技術分別構建其可溶性原核表達載體和不溶性原核表達載體；用pcr快速檢測法及其基因測序儀測序以鑒定pet22b hpk - 5和pbv220 hpk - 5重組質粒，用不同的感受態大腸桿菌（ eIn this experiment hcv structural gene was amplified by polymerase chain reaction ( pcr ), and was inserted into baculovirus expression vector pfastbacl to construct a recombinant transposing vector pfbl - cee. the plasmid pfb 1 - cee was transformed into dh1 obac competent e. coli cells. high molecular weight dna was prepared from the overnight cultures from the selected e. coli colonies, which was recombinant baculovirus shuttle vector containing hcv structural gene, named bac - cee
本實驗用pcr擴增hcv結構區基因，克隆到桿狀病毒表達載體pfastbacl中，構建成重組轉座載體pfb1 - cee ，轉化dh10bac大腸桿菌感受態細胞，篩選陽性菌落，抽提大分子質粒dna ，獲得含hcv結構區基因的重組桿狀病毒穿梭載體bac - cee ，脂質體介導轉染sf9昆蟲細胞，出現細胞病變后，收集含有重組桿狀病毒顆粒的培養上消，重新感染sf9細胞，收集sf9細胞，進行12 . 5 sds -聚丙烯酰胺凝膠電泳，可見表達的蛋白條帶。In order to further investigate the role of axudl in human tumor carcinogenesis and the potential association between the axudl gene expression status and the stimulation of transforming growth factor beta in human cancers, the present study was performed in three aspects as follows : ( 1 ) cloning full length enconding region cdna of axudl and construction of eukaryotic vector that expression the fusion protein of axud1 and influenza virus hemagglutin ha epitope tag ; ( 2 ) exploring the time and dose effects of tgf - 1 on the expression - of axudl gene in hepg2 hepatoma cells and spc - a1 lung carcinomas cells, and studying the effects of overexpression of axud1 on the expression of cell cycle and apoptosis related protein in hepg2 hepatoma cells ; ( 3 ) construction and expression of human axudl in e. coli m15. the following main results and conclusions can be obtained from the present study : 1. the full length ecnoding region of human axudl cdna from human peripheral blood lymphocytes was successfully cloned using one step rt - pcr method, and constructed into a eukaryotic expression vector which can be expressed a ha - axud1 fusion protein with axud1 and influenza virus hemagglutin ha epitope tag. the recombinant plasmid was identified by polymerase chain reaction, restriction endonuclease maping and sequencing, this expression vector might be instrumental to further study the function of axud1 protein in tumor cells
為了進一步研究axud1在人類腫瘤發生中的作用及axud1基因的表達狀況與tgf -介導的信號通路的關系，本實驗研究分為三個部分： （ 1 ） axud1基因cdna全長編碼區的克隆和ha表位標記的axud1基因表達載體的構建； （ 2 ）探討肝癌細胞hepg2和肺腺癌spc - a1細胞中tgf - 1誘導的axud1基因表達的時間、劑量效應以及誘導表達的可能機理，並研究axud1的過表達對細胞周期和細胞凋亡相關蛋白表達的影響； （ 3 ） axud1原核表達載體的構建及其在大腸桿菌中的表達。本實驗的主要結果和結論如下： 1利用一步法rt - pcr成功地從人類外周血淋巴細胞中克隆出axud1基因編碼區cdna ，並將其構建入真核表達載體中，編碼的ha - axud1融合蛋白帶有流感病毒凝血素ha的表位標記肽段。Based on the analysis of present situation, this paper advances the approaches to maintaining advanced nature of the party membership in colleges and universities : firstly, improving political awareness through persistent party spirit education ; secondly, improving the sense of honor and responsibility by cultivating campus culture ; thirdly, providing the system safeguard by strengthening the evaluation mechanism ; fourthly, innovating the carrier of advanced nature maintaining with the image improvement program ; fifthly, enhancing the self - discipline by implementing self - education, self - management and self - supervision
筆者從黨員教師先進性要求的內涵及現狀分析著手，提出保持高校黨員先進性的對策： （ 1 ）持之以恆地開展黨性教育，提高黨員教師的思想政治素質； （ 2 ）努力營造校園環境，提高黨員教師的政治榮譽感和責任感； （ 3 ）健全考核機制，為保持黨員個體先進性提供製度保障； （ 4 ）實施黨員形象工程，創新黨員保持先進性的載體； （ 5 ）啟動自我教育、自我管理和自我監督標桿，增強黨員教師自律意識。分享友人