抗重鏈抗體 的英文怎麼說

中文拼音 [kàngzhòngliànkàngtǐ]
抗重鏈抗體 英文
anti-heavy chain antibody
  • 抗 : Ⅰ動詞1 （抵抗; 抵擋） resist; combat; fight 2 （拒絕; 抗拒） refuse; defy 3 （對等） contend with...
  • 重 : 重Ⅰ名詞（重量; 分量） weight Ⅱ動詞（重視） lay [place put] stress on; place value upon; attach im...
  • 鏈 : Ⅰ名詞1. （鏈子） chain Ⅱ動詞（用鏈栓住） chain; enchain Ⅲ量詞（計量海洋上距離的長度單位） cable length
  • 體 : 體構詞成分。
  1. Culture of mg7 hybridoma cells and detection of antigen - binding affinity of mg7 mab by elisa 2. construction and identification of mg7 recombinant phage antibody library mrna was isolated from cultured mg7 hybridoma cells and converted into cdna ; the variable fragments of heavy and light chain were separately amplified and assembled into scfvs with a specially constructed dna linker by pcr. the scfvs dma was ligated into the phagmid vector pcantabse and the ligated sample was transfered into competent e. co / / tg1 to generate a bacterial form of mg7recombinant phage antibody library ; volume and recombinant ratio of the library were evaluated by means of bacterial colony counts and restriction analysis ( ecor i and hind iii )

    Mg _ 7重組噬菌體抗體庫的構建及鑒定從培養的mg _ 7雜交瘤細胞中提取並分離mrna ，反轉錄成cdna ；利用pcr分別擴增mg _ 7單抗的重鏈及輕鏈可變區基因，並通過? dna連接子將二者連接起來形成mg _ 7單鏈抗體基因；將mg _ 7單鏈抗體基因插入pcantab5e ；將連接產物轉化感受態tg1大腸桿菌，制備細菌形式的mg _ 7重組噬菌體抗體庫；通過菌落計數和限制性酶切分析（ ecor和hind ）評估mg _ 7重組噬菌體抗體庫的容量和重組率。
  2. Thus, immunologists have sought smaller molecules with the antigen - recognition capability of antibodies. the variable domains of the heavy ( h ) and light ( l ) chains are sufficient for antigen recognition but the non - covalent complex of the two variable domains ( fv ) is unstable. it is possible to genetically engineer a single - chain fv ( scfv ) with the h chain v region connected to the l chain v region via a 15 amino acid linker composed of serine and glycine amino acid residues

    二、日本血吸蟲單克隆杭獨特型抗體np30的單鏈狐（ scf ）的構建、表達及對balbic小鼠誘導保護性作用研究l 、日本血吸蟲單克隆杭獨特型抗體np0的單鏈抗體歸cfv ）的構建、表達通過pcr方法體外擴增並經測序驗證的重鏈、輕鏈可變區（ vh 、 vl ）基因先後重組入原核表達質粒ptha90相應的位點上，中間通過一連接肽（ gly在er ） 。
  3. Recovery estimated from the safh4 plant line indicates that 9 ( jl g of pure active scfv can be obtained per gram of fresh leaf material, on a laboratory scale. the production of the scfv antibody proteins in plant root exudates was also addressed. the scfv antibody protein was continuously secreted from the transgenic tobacco roots into a simple hydroponic medium at 630 to 760 ng g - 1 dry weight of root day - 1

    在水培條件下，轉基因煙草根可連續分泌具有活性的重組抗乙肝病毒表面抗原pres1 （ 20 - 47 ）單鏈抗體進入到液體培養液中，不須破壞植物即可連續獲得重組單鏈抗體，為利用植物生物反應器連續生產單鏈抗體開辟了新途徑。
  4. For bus system installation, the focus is put on cable selection principle, installation process and antinoise measure ; for security protection equipment installation, the intrinsic safety concept, installation method equipment selection and installation under two electric power supply modes ( entity concept and fisco model ) and the bus system protection against surge voltage are illustrated ; for network component installation, the selecting, installing and integrating methods of general purpose terminators, repeaters, link equipment, bus power supply and impedance module are described

    在總線電纜的安裝方面，著重討論了電纜的選型原則、安裝方法和抗噪防擾措施；在安全保護設備安裝方面，介紹了總線系統中本質安全的概念、安裝方法、兩種供電方式（實體概念和fisco模型）下設備的選擇與安裝以及總線系統浪涌電壓的保護和設備安裝方法；在網路元件的安裝方面，主要列舉了通用終端器、中繼器、鏈路設備、總線電源及其阻抗模塊等的選擇、安裝和集成方法，為總線系統的安裝提供了可以借鑒的實用經驗。
  5. New york ( reuters health ) dec 19 - lactobacilli transformed to carry heavy - chain antibody fragments appear to protect against rotavirus infection, swedish investigators report in the december 1st issue of the journal of infectious diseases

    紐約（路透社健康版） 12月19日瑞典研究人員首次在12月傳染病雜志上發表報告稱：經改造攜帶重鏈抗體片斷的乳酸菌有抗輪狀病毒感染的作用。
  6. The mitochondria presents several defense systems, of which the ubiquinone ( coenzyme q10 ), which is one of the components of the respiratory chain and which has a significant antioxidant function acting upon membrane proteins

    線粒體具有幾種防禦系統，泛醌（輔酶q10 ）是呼吸鏈的組成成分之一，在膜蛋白質上具有重要的抗氧化功能。
  7. This is important in attempting to use antibodies as anti - tumor reagents, for example

    基因為日本血吸蟲單克隆抗獨特型抗體np30輕鏈和重鏈可變區基固。
  8. Lts membrane properties and effect on cross - iinking biomoiecuiars were discussed. protein a was selected as a sensing membrane owing to the fact that it contains a active spot which can bind fc portion of antibodies. so the orientated immobilization of antibody was achieved

    丁二烯-苯乙烯共聚物作為膜材料，研究了膜的性質和對生物大分子修飾的效果；另外，對具有專一性結合抗體fc功能區的蛋白a作為中間鏈接層進行了膜性質、定向修飾功能、重復性方面的研究。
  9. The library was rescued with phage m13k07 in order to display scfv on the surface of the phage and to form the recombinant phage antibody library. one of positive scfv clones, named pcsal, was selected with phage - elisa after panning and screening by bull sperm three times. scfv fragment, amplified from pcsa1, was ligated to pmd18 - t vector for sequencing analysis

    取陽性重組噬菌體抗體克隆株pcsa1 ， pcr擴增其scfv基因，篩選重組子進行序列測定，發現其序列符合小鼠抗體基因的一般特徵，並且與幾株抗磷酸膽堿的抗體重鏈和輕鏈可變區序列的同源性達80以上；推測pcsa1scfv針對的抗原是磷酸膽堿類物質。
  10. In the present study, the express library of monoclonal anti - sp18 scfv ( single chain fragment variable ) gene is constructed and selected for further study of sp18 antigen on mammalian fertilization and embryogenesis. total rna were firstly isolated from these growing hybridoma cells which secretes monoclonal anti - sp18 antibodies. after obtained using rpas system, vh and vl genes were used to assemble scfv gene fragment with a linker primer

    應用重組噬菌體抗體庫技術，從分泌小鼠抗牛精子sp18抗體的雜交瘤細胞系中分離總rna ，克隆抗體重鏈和輕鏈可變區基因，加入連接肽引物（ linkerprimer ）組裝成單鏈抗體scfv （ singlechainfragmentvariable ）基因並用rs引物進行擴增， sfi 、 not酶切，回收后與pcantab5e載體相連，轉化e . colitg1宿主菌，構建單鏈抗體文庫。
  11. Methods : a set of oligonucleotide primers were designed and used to amplify the vh and vl gene from anti - hbsag fab antibodies screened from phage antibody library. the products were cloned into puc19 vector and their sequences were analysed. the vh and vl gene fragments were tethered by a peptide linker and a leader sequence coding region, with the leader sequence added at 5 " terminus of each gene ( l - vh - linker - vl ) and designated as l - scfv

    方法以從噬菌體抗體庫中篩選獲得的抗hbsag的fab抗體基因為模板，分別擴增出其輕、重鏈可變區（ v _ l 、 v _ h ）基因，通過重組pcr方法將輕、重鏈可變區基因用連接肽（ gly _ 4ser ） _ 3的編碼序列連接，並引入前導肽編碼序列，構建具有l - v _ h - linker - v _ l結構的單鏈抗體基因。
  12. They found that np30 had partial cross - reaction with sea and maa and could be used as " antigen reagent " in the immunodiagnostic assays based on antibody detection of schistosomasis japonica. it is difficult to obtian gaa by genetic engineering methods, because gaa is glycoprotein

    基固，擴增產物鑒定后，將其克隆apuc19載體，重組子用san驢r 』 s雙脫氧鏈終止法和自動測序法測定其序列，序列與genebank中及已發表的抗體序列進行比較分析。
  13. Expression levels of scfv antibodies reached up to 0. 5 % of total soluble proteins in leaves. the biologically active scfv was easily purified ( to 90 % purity ) from safh4 plant material using immobilized metal ion affinity chromatography

    以固相金屬離子親和色譜法，從轉基因煙草中一步分離到純度達90的具有生物活性的單鏈抗體，每克葉片組織可獲得9 g重組蛋白。
  14. After dissolving inclusion bodies, renaturing inclusion bodies and further purification by immobilized metal ion affinity chromatography ( imac ), the fusion protein trx - scfv of electrophoretic purity was obtained, they still possessed antigen - binding activity

    經變性復性，用固相金屬離子親和色譜法一步分離純化了具有功能的單鏈抗體，在一升的發酵液中得到78mg的重組蛋白。
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