提克達 的英文怎麼說
中文拼音 [dīkèdá]
提克達
英文
bisanthe-
The kremlin ' s security council ended months of acrimonious debate on thursday and backed cuts of some 600, 000 people over five years
克里姆林宮的安全委員會在本周四結束了常達數月的激烈爭論,通過了這項在五年內裁軍60萬的提議。Screening target ' gene of artemisinin antimalarials using drug - western from cdna expressing library : 12 b - deoxoartemisinyl - ( 4 ' - oxyacetic acid ) phenyl ether was linked to bsa by using of edc cross ! inker and the product acted as drug - probe
對重組pmd一18一t克隆載體及pqe一30表達載體雙酶切,提取tctp基因和pqe一30空載體並使二者重組,然後轉化m15 ,挑取陽Methods : an artificial gene for fl extracellular domain cdna was synthesized by using favored genetic codons of pichia pastroris. by inserting human fl extracellular domain cdna coding 156 amino acid resi dues into pichia pastoris expression vector ppic9k containing aox1 promoter and the sequences of alpha secreting signal peptides, a recombinant expression plasmid ppic9k - fl was constructed, and integrated into the alcohol oxidase region of the host genome
為了提高外源基因的表達量,我們根據畢赤氏酵母偏愛密碼子人工合成了編碼fl胞外區156個氨基酸的cdna序列,目的序列被定向克隆到酵母分泌型表達載體ppic9k質粒上,構建ppic9k - fl表達質粒。Lots of suggestions were put forward, including two extremely bankable young american stars : leonardo dicaprio and matt damon, along with a slightly older one, tom cruise
很多建議被提舉出來,包括兩個非常有投資收益潛力的年輕美國演員:萊昂納多和馬特達蒙,還有稍微大一點的湯姆克魯斯。In this paper, first strand cdna of 3abc gene was synthesized using template rna extracted from cells infected with fmdv. the complete 3abc gene about isoobp was amplified by pcr and ligated into pgem - t easy vector. after transforming e. coli dh5 a, ampicillin resistant colonies were isolated and plasmid dna was prepared and analyzed by restriction analysis and pcr. presence of the full length 3abc gene was verified by nucleotide sequence analysis and the plasmid containing the expected sequence was named as pgem - 3abc. comparing the aquired sequence of 3abc with that of reference strains, the homology is more than 99 percent. the pgem - 3abc was digested with sal i and bgl ii and ligated into xho i and bgl ii - digested expression vector ptriex - 4 neo. lt was identified by restriction analysis and pcr and sequencing that this fragment had a 17bp deletion hi the nucleotide sequence 708bp of 3abc gene, which happened to form a terminator codon behind 3ab gene, but it contained the complete open reading frame ( orf ) of 3ab gene. positive clones were selected and induced with lmmol / l isopropyl - d - galactoside ( iptg ), bacteria were detected by sds - page and western blotting after properly treated. the results showed that the 3ab gene expressed successfully in e. coli and 33. 5ku fusion protein can be recognized by the positive bovine serum of fmdv. the amount of target protein is over 26 % of the total bacteria protein by gel thin layer scanning analysis
擴增產物連接到pgem - teasy載體中,轉化大腸桿菌dh5菌株,篩選氨芐青霉素抗性菌落,提取質粒經酶切鑒定、 pcr分析以及確證性測序證明,所克隆的1500bp左右的片段含有完整的3abc基因,與國外參考序列相比,同源性在99以上。將重組質粒pgem - 3abc和表達載體ptriex - 4neo分別用sal和bgl與xho和bgl消化后,亞克隆3abc基因至原核表達載體ptriex - 4neo中,通過酶切鑒定、 pcr擴增以及序列分析,發現克隆到ptriex - 4neo載體上的片段於3abc基因708bp處出現了17bp的缺失,碰巧在3ab基因后形成一終止密碼子,但3ab基因的閱讀框架完整,選出含有3ab基因完整閱讀框架的陽性克隆,用iptg誘導表達,收集菌液進行sds - page電泳、 westernblotting分析,結果表明, 3ab基因在大腸桿菌中成功表達,其表達產物為分子量33 . 5ku的融合蛋白,並能被口蹄疫病毒陽性血清識別。經薄層掃描分析,表達量占總蛋白量的26以上。The superior armor protection of the heavy cast turret on the kv - 1 model 1942 tank has its cost ! the weight of the tank now reaches 47 tones and this weight are consider heavy even for a modern tank standard
Kv - 1 1942年重型鑄造炮塔坦克雖然防護能力得到提高,但是車體重量增加的問題又出現了,其戰斗全重達到了47噸,這個重量在現代的坦克而言都是很重的。Many studies show that leafy is high homolog even among distantly related plant species. exception of these, little studies on tissue culture and transformation of ginkgo have been done. this paper emphasizes on the isolation, cloning and analysing two ginkgo orthologs of leafy from the male tree
為此,本實驗從銀杏leafy同源基因的克隆入手,分析其雌雄株lfy基因結構差異,構建lfy基因的植物正義反義表達載體,建立矮牽牛遺傳轉化體系,以研究銀杏lfy同源基因的功能,同時建立了銀杏組織培養體系,為銀杏的遺傳轉化和提早開花結果奠定基礎。Porcine transmissible gastroenteristis is an importan contagious disease endangering the development of swine. in other to establish a rapid diagnosis method and provide effective immunogenic products, the nucleoprotein ( n ) gene of porcine transmissible gastroenteristis virus ( tgev ) was cloned. expressed and its expressed product was purified
為建立對豬傳染性胃腸炎快速有效的診斷方法,並試圖在預防上提供有效的免疫制劑,本論文首次在我國對豬傳染性胃腸炎病毒核衣殼蛋白基因進行了克隆、鑒定、表達及重組核蛋白的純化;並在細胞上對重組核衣殼蛋白抗體的中和效力進行了測定。This part is set up for analyzing systematically the relationship between rli and rural economic development, summarizing the influence law of rli to rural land management benefit, analyzing the innovative mechanism, institutive achievement and difficult position and gauntlet of the present rli in china so as to lay a practical foundation for the innovation design of rli. the third part from chapter 6 to chapter 8 is innovation. in this part, the binding factors, innovative objective and basically guiding ideology of present rli innovation have been analyzed first, then according to the basically theory that relations of production must be suited to productive forces and the basically demands of socialist market economic system, in the light of peasant ' s receptive level and receptive capacity, the innovative model by stages of rural land property rights has been made, finally, the relationship between family farming and farming in scale has been analyzed, the latent profit and its binding factors of present family farming have been expounded, the concrete measures of perfecting family farming institution and pushing on the farming in scale have been put forward
第六章根據制度創新理論和市場經濟理論,結合我國國情,論述了現階段我國農地制度創新的制約因素、創新的目標及基本指導原則,確定了我國農地制度創新的方式;第七章根據生產關系必須適應生產力發展水平的馬克思主義基本原理和建立社會主義市場經濟體制的要求,結合我國現代化建設分「三步」走的戰略目標及農民的接受程度和能力,認為我國未來的農地制中文摘要度不應該拘泥於某一固定模式,而應該建立在系統連貫性的動態優化組合和階段性漸進演化的基本格局之中,並構建了我國農地產權制度的階段性創新模式:第八章通過對農業生產的特點和家庭經營的優勢分析,結合發達國家農業發展的經驗,提出了家庭經營是我國21世紀農地經營的最佳組織形式的結論;論述了農業家庭經營與農地規模經營的關系,分析了現階段我國農業家庭經營的潛在收益及其實現的制約因素,進而提出了完善農業家庭經營制度和推進農地適度規模經營的具體措施。Saddam hussein proposed the most complete self - government plan ever offered to the kurdish people in northern iraq
薩達姆?海珊提議給伊拉克北部的庫爾德人完全的自治權。Gene engineering based on reducing na + toxicity creats a new approach to plant salt tolerance. this is the first time expressing of sod2 in arabidopsis thaliana and maybe attribute to research the ion homeostasis in the cell. the sod2 gene was isolated from s. pombe by rt - pcr method and confirmed by sequencing
雖然sod2基因已經克隆,但是sod2在高等植物中異源表達及對其影響還未見報道,因此sod2基因在擬南芥中過量表達,對研究細胞的離子均衡及運用基因工程手段有效地提高植物的抗逆性具有重要意義。Plants are thought to remove na + from the cytoplasm by transporting it into the vacuolar or out of the cell using na + / h + exchangers localized in the vacuolar and plasma membranes, respectively. sos1 encoding a plasma membrane na + / h + antiporter and atnhxl encoding a vacuolar na + / h + antiporter were isolated from glycophytic arabidopsis thaliana, and overexpression of atnhxl and sos1 in arabidopsis thaliana increased the salt tolerance of transgenic plants significantly
目前,擬南芥細胞內控制na ~ +外排的基因sos1及離子區隔化基因atnhx1均已克隆, sos1及atnhx1在擬南芥中的過量表達顯著提高了轉基因植株的耐鹽性,開創了降低na ~ +毒害的基因操作新途徑。An iraqi spokesman confirmed that saddam ' s half brother, barzan ibrahim al - tikriti, and the former head of iraq ' s revolutionary court, awad hamed al - bandar, were hanged before dawn on monday
伊拉克政府發言人證實,薩達姆同母異父的兄弟巴爾贊.易卜拉欣.提克里提以及前伊拉克革命法庭的負責人阿瓦德Though below average height and not particularly handsome in the traditional sense, within 5 years tom cruise was starring in some of the top grossing films of the 1980s including top gun 1986 ; the color of money 1986 ; rain man 1988 and born on the fourth of july 1989. by the 1990 s he was one of the highest paid actors in the world earning an average 15 million dollars a picture in such blockbuster hits as interview with the vampire : the vampire chronicles 1994 ; mission : impossible 1996 and jerry maguire 1996 for which he received an academy award nomination for best actor. in 1990 he renounced his devout catholic beliefs and embraced the church of scientology claiming that scientology teachings had cured him of the dyslexia that had plagued him all of his life
就在壯志凌雲大獲成功的1986年,他與影壇宿將保羅-紐曼共同主演了金錢本色color of money ,由他飾演年輕氣盛的撞球天才文森特,該片為保羅-紐曼帶來了一尊奧斯卡金像1988年,他在雨人rain man一片中飾演「雨人」的弟弟查理雖然評論界都把注意力集中在主演達斯廷-霍夫曼的身上,但查理這個不斷轉變的角色較之一直呆頭呆腦的「雨人」更具難度1992年,克魯斯又與傑克-尼科爾森在法庭片好人寥寥few good ma n中大唱對臺戲,該片也獲得了奧斯卡獎提名。A rescue worker stands on the grounds of the vast petrochem ? ical complex in the port city of skikda, january 20, 2004, following a huge explosion on the evening of january 19
1月20日,一名救援人員站在斯基克達(阿爾及利亞一個省城)液化天然氣提煉廠爆炸現場。" we know where they are. they ' re in the area around tikrit and baghdad and east, west, south and north somewhat.
"我們知道它們(大規模殺傷性武器)在哪兒.他們在巴格達與提克里特之間,東,西,南,北各個方向.At least 15 people were killed and 8 others injured late sunday while a rocket slammed into a target in iraq ' s northern city of tikrit, the us military and local hospital said
當地時間10月31日,伊拉克前總統薩達姆家鄉提克里特的一家飯店遭到火箭襲擊,造成15名伊拉克人死亡,大約8人受傷。Ifyou have a message for mark darcy, speak after the tone
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