提純柱 的英文怎麼說

中文拼音 [chúnzhù]
提純柱 英文
decontaminating column
  • : 提動詞(垂手拿著) carry (in one's hand with the arm down)
  • : 形容詞1 (純凈; 不含雜質) pure; unmixed 2 (純粹; 單純) simple; pure and simple 3 (純熟) skil...
  1. 6 - phosphogluconate dehydrogenase ( 6 - pgadase, ec 1. 1. 1. 44 ) was isolated by homogenate, ammunium sulfate fractionation, deae - sepharose chromatography, blue - sepharose affinity chromatography and gel filtration with sephadex g - 200 from bacillus subtilis, and some properties of the enzyme had been studied. a 113. 8 - fold purification was obtained with a 8. 2 % yield. the purified enzyme moved as a single electrophoretic band in page

    將枯草芽孢桿菌超聲波破壁后的粗取物進行分段鹽析、 deae - sepharose陰離子交換層析, blue - sepharosecl - 6b特異結合層析和sephadexg - 200凝膠過濾等化步驟,得到聚丙烯酰胺凝膠電泳為單一蛋白區帶,比活為1 . 46u mg的酶制劑。
  2. This enzyme was different with the ones reported in the past. a phosphatase was isolated from the chloroplast thylakoid membrane of ipomoea aquatica, by nacl extration, ammonium sulfate precipitation, ion - exchange chromatography and hydrophic chromatography through butyl - toyopearl 650m column

    使用nacl抽、硫酸銨分步沉澱、離子交換和butyl - toyopearl650m疏水層析等方法,從蕹菜葉綠體類囊體膜中分離化到一種蛋白磷酸酯酶。
  3. In this paper, by shorting the length of deae - cellulose column and increasing the ph of elution solution, one kind of go isozyme was purified rapidly from green leaves of spanictu brassica parachinensis bailey and vigna unguiculatew. ssp. sesquipedalis ( l )

    本文通過一種改進后的方法,即通過縮短deae - cellulose - 52的長度,以及高洗脫液的ph ,可在9h內從菠菜、菜心和豆角綠葉中化go同工酶。
  4. The method for determining avermectin ( avm ) in muscle of sturgeon ( a. schrenckif ) by high performance liquid chromatography with fluorescence detector was established. the homogenized muscle sample was extracted by acetonitrile, following centrifugation at 3000 g for 15 min

    採用乙腈作為肌肉中蛋白沉澱劑和阿維菌素的取劑,經堿性氧化鋁spe化,蒸乾和衍生化,用高效液相色譜( hplc )檢測。
  5. Then enzyme was purified with a deae - cellulose ( 5. 5x50cm ) column, a toyopearl hw - 65 ( 5. 5 x 50cm ) column and a sephadex g - 200 ( 5. 5 x 80cm ) column. finally, the enzyme was purified for 10 folds with the recovery of 17. 4 %. page showed a single band for the purified creatinase

    3 、肌酸水解酶的酶在硫酸銨飽和度為40 80之間完全沉澱,先後經過deae - cellulose離子層析、 toyopearlhw - 65疏水層析、 sephadexg - 200分子篩層析層析,最終使酶10倍,最終得率為17 . 4 。
  6. Get the inclusion bodies 2 ) western blot analysis of fusion protein expression ( 1 ) electrophoresis ( 2 ) transfer proteins from gel to membrane ( 3 ) blocking ( 4 ) incubation with primary antibody ( 5 ) enzyme conjugate incubation ( 6 ) substrate incubation. 3 ) gst detection module with cdnb enzymatic assay 3 purification of gst fusion proteins 1 the denaturalization of inclusion bodies. 2 purification using glutathione sepharose 4b column wash matrix with 1 pbs, prepare a 50 % slurry for batch purification method, pack column with matrix slurry

    三、 gst一hbrp重組蛋白的化1 .超聲破碎細胞,離心,上清和沉澱進行sds一page電泳分析2 .樣品處理取包涵體,變性后,加人用pbs平衡過的以utal腸onesepb抓脫4b ,室溫下孵育3 .以utadtionesepharose4b化以utad雲onesepharose4b的準備根據毛山lel ,決定化所需的gll衛ta1如oneseph娜se4b的床體積,用預冷的1xpbs清洗cldtathionese戶, se4b ,得到50 %的基質
  7. Foodstuffs - determination of ochratoxin a in wine and beer - hplc method with immunoaffinity column clean - up

    食品.葡萄酒和啤酒中赭麴黴素a的測定.免疫親和高效液相色譜法
  8. Foodstuffs - determination of ochratoxin a in barley and roasted coffee - hplc method with clean - up on a immunoaffinity column

    食品.大麥和炒制咖啡中赭麴黴素a的測定.免疫親和高效液相色譜法
  9. This material was loaded onto a silica gel column and the column was eluted with chloroform several times until the fraction of interest was collected. finally we obtained some light yellow green ointment liquid f i

    採用硅膠作干層析填充物,用氯仿作展開劑和洗脫液,反復硅膠層析,對柄海鞘鞘囊氯仿浸物進行分離、取與化,最後得到一淺黃綠色的油狀液體fraction (以下均稱f ) 。
  10. According the key factors we find, we bring forward a new conception : multilevel suppressor and design a new high performance suppressor whose ion - exchange membrane has bigger areas and using three electrodes including one cathode ( anode ) and two anodes ( cathode ), at the same time we fill the suppression compartment with one kind of ion exchange resin which has moderate exchange capacity. according to our experiment ' s results, we find the new type suppressor has quite high working current efficiency and suppressing capacity. in most cases, the suppressor ' s current efficiency is over 90 % ; the suppressor can transform the naoh ( concentration : 200mmol / l, flow rate : i. oml / min, conductance : over 10000 i - i s cm " ) to pure water ( conductance : 8. 9 it s cm in chapter 3, the high performance suppressor is applied in determination some trace - amounts ions in plating solution, sewage. in this chapter, we also have a research on the gradient ion chromatography

    第二章首先以xyz - 1型電化學抑制為例,分析了電化學抑制的抑制過程得出影響抑制容量的主要因素主要是抑制的電流效率和離子交換膜的極限電流密度,因此採用中等交換能力的離子交換樹脂作為抑制室的填料以高電流效率,在通常情況下電流效率可達到90以上;在選用同種離子交換膜的前下,可通過增加離子交換膜的有效面積達到高極限電流的目的從而高抑制的抑制容量,因此出了多級抑制的概念並據此研製了共電極式高容量電化學抑制,該抑制最高可將流速為1 . 0ml / min ,濃度為200mmol / l電導率超過10000 s ? cm ~ ( - 1 )氫氧化鈉溶液抑制為電導率低至8 . 9 s ? cm ~ ( - 1 )的水,並且具有穩定性高、分析結果準確等優點。
  11. The isolation and purification of dnaase in the earthworm the earthworm dnaase was purified from the tissue extract of earthworm by denaturing the protein with low ph buffer, high temperature, ammonium sulfate precipitation, deae - cellulose ( de52 ) chromatography and ultra - filter membrane

    雙胸蚓組織中dna酶的分離化雙胸蚓組織粗取液經過選擇性酸變性、選擇性熱變性、硫酸按分段鹽析、 deae一纖維素( de52 )層析、超濾膜分級分離后得到一個電泳的dna酶。
  12. Conclusion the co - utilization of silica gel column and gel chromatography can greatly improve the efficiency of isolation and purification

    結論硅膠色譜和凝膠色譜的混合利用可以高供試品的分離化效果。
  13. The bioactive strain ' s fermentation product was isolated and purified primarily using methods of solvent extraction, acid - alkali extraction, ab - 8 macropore absorption chromatography, 1 x 007 positive ion exchange chromatography, the result showed the purification product has prominent bioactivity inhibit staphalococcus aureus

    中國熱帶農業科學院、華南熱帶農業大學2003屆碩士研究生採用有機劑抽法、酸堿抽法、大孔吸附樹脂層析和陽離子交換樹脂層析等方法對hsl 306菌株發酵產物進行了初步分離化。
  14. Abstract : in this paper, a kind of technological scheme for one - step precision forging spur gear with large module is put forward by using positive friction role. experiment, in which commercial pure lead is taken as test material, is done in the special die designed, and force vs. stroke curve is recorded. it is shown that positive friction role can efficiently make a contribution to the filling of the tooth cavity, but increase forming force

    文摘:針對大模數直齒圓齒輪,出了利用積極摩擦一步成形的工藝方案,並用工業鉛作為試驗材料,在設計的專用模具上進行試驗,記錄了力? ?行程曲線,結果表明:利用積極摩擦作用能夠有效地保證直齒圓齒輪精鍛時齒腔充滿,但同時增加了變形力。
  15. To isolate and purify dnaase in the earthworm first, the tissue extract of earthworm was prepared by dissolving the earthworm with sucrose and denaturing the protein with low ph buffer. then dnaase was purified by denaturing the protein with higher temperature. the following steps were ammonium sulfate precipitation, deae - cellulose ( de52 ) chromatography and filtration by ultra - filter membrane

    雙胸蚓組織中dna酶的分離化採用蔗糖溶解雙胸蚓,並選擇性酸變性制備雙胸蚓組織粗取液,再經選擇性熱變性、硫酸銨分段鹽析、 deae ?纖維素( de52 )層析及超濾膜分級分離對雙胸蚓組織中dna酶進行分離化。
  16. Herba ephedrae was saturated with 0. 5 % hydrochloric acid, the extract was extracted with ether and chloroform respectively, from which ephedrine was separated and purified by column chromatography

    以0 . 5 %鹽酸浸泡麻黃草,分別用乙醚、氯仿萃取麻黃粗物,通過色譜法分離麻黃堿。
  17. This paper presents the effects of some features on the productivity of raw c60 materials, such as distance and approaching speed of electrodes, helium partial pressure and arc current etc. then we separate and purify the raw materials and obtain pure solid c60 of 99. 9 % and compare the purification efficiency and effect of different fluxion phase and fixed phase and discuss the effects of the experimental conditions, such as the depositing speed, the type of the substrate, the surface structure of the substrate and the temperature of the substrate. finally, we use xps, afm, ultraviolet, infrared and raman to analyze the component, structure and feature of the films qualitatively and quantitatively

    本文首先研究了氦氣分壓、弧電流大小、電極間距以及電極推進速度等實驗條件對制備c _ ( 60 )粗品產率的影響;接著選用色譜法分離得到了度大於99 . 9的c _ ( 60 )固體,比較了不同流動相和固定相的效率和效果;然後採用自己改進后的真空鍍膜機,利用電阻式加熱蒸鍍方法,得到了c _ ( 60 )薄膜和不同摻雜比的銀摻雜薄膜;探討了沉積速率、襯底種類、襯底表面結構以及襯底溫度等實驗條件對薄膜結構的影響;最後通過xps , afm ,紫外,紅外,拉曼對薄膜的成分、結構和特性作了定性和半定量分析。
  18. The enzyme retained full activity after being treated at room temperature for 1 hour at ph between 4. 0 and 11. 5. the enzyme can be incubated at 50 for 4h with only less 50 percent loss of activity and is stable in the frozen state. when streptomyces griseus atcc14811 was cultured in 10. 3 % sucrose yeme liquid medium, production of extracellular cholesterol oxidase increased for 5 days before decrease

    利用硫酸銨鹽析及deae -纖維素離子交換層析化灰色鏈黴菌atcc14811發酵上清液中的膽固醇氧化酶,理化性質研究表明酶作用晟適ph為8 . 0 ,最適溫度為45 , ph穩定范圍在ph4 . 0 - 11 . 5之間,在50條件下保溫4h ,仍保留54酶活力。
  19. Under the horizontal earthquake action and wind force, aim at " the pure frame structure with rectangle columns ", " the pure frame structure with special - shaped columns ", " the frame - truss structure with special - shaped columns " and " frame - shear wall structure with special - shaped columns ", earthquake action analysis was done by the spacial finite element method through the changes structural parameter. analyzing systematically " structure vibration mode ", " vibration period ", " structure lateral rigidity ", " seismic action force ", " seismic response force ", " floor seismic shear force ", " lateral horizontal displacement of structure " and " members internal force ". results indicate : ( 1 ) based on equal area, the special - shaped columns replaced the rectangle columns, the structural lateral rigidity enlarges, the lateral displacement minishes obviously, the earthquake response increase slightly, the biggest increasing amount of frame columns axis - compress ratio is smaller than 0. 08. the whole aseismic performance of structure has improved ; ( 2 ) when the section ' s length and section ' s thickness ratio of special - shaped columns is smaller than 3. 6, the structure benefits to resist seismic action ; ( 3 ) the angle of horizontal seismic action with the whole coordinate is 0 degree, structure earthquake response is bigger, belonging to a control factor of structure aseismic design ; ( 4 ) the frame - truss structure with special - shaped columns and the " a " - brace has the biggest lateral rigidity ; ( 5 ) the frame - shear wall structure with special - shaped columns have bigger lateral rigidity and smaller displacement, members internal force enlarged just rightly, have much superiority of resisting seismic action ; ( 6 ) in the higher seismic fortification criterion region ( 8 degree of seismic fortification intensity ), aseismic disadvantageous building site ( iii type site ), adopting special - shaped columns structure system, should reduce possibly the building ' s self - weight in order to reduce the earthquake response ; ( 7 ) the response spectrum method of computing seismic response and the time - history analysis method have similar analysis result

    西安理工大學碩士學位論文在水平地震作用下,並考慮風荷載組合,分別對「矩形框架結構」 、 「異型框架結構」 、 「異型框一析架結構」及「異型框-剪結構」等四種結構體系,通過改變結構參數,運用空間有限元方法,進行地震作用計算。系統地分析研究「結構振型」 、 「振動周期」 、 「結構側向剛度」 、 「結構地震反應力」 、 「樓層地震剪力」 、 「結構側向位移」 、 「層間位移角」 ,以及「構件內力」 。結果表明:在等面積原則下,異型代換矩形后,結構剛度增大,側向位移明顯減小,地震反應力略有增加,框架軸壓比最大增幅小於8 % ,結構整體抗震性能有所高;當異型肢長肢厚比小於等於3 . 6時,結構有利於抗震;水平地震作用力與整體坐標夾角為0度時,結構地震作用效應較大,屬結構設計的控制因素之一;異型框一析架結構採用「人」字斜撐,側向剛度大於「八」字斜撐和「人一八」字混合斜撐;異型框一剪結構側向剛度大、位移小,構件內力增大適中,是一種抗震性能優越的結構體系:在抗震設防烈度較高地區( 8度)和抗震不利的建築場地( m類場地) ,採用異型結構體系時,應盡可能減輕結構自重,降低地震作用力;分別採用「振型分解反應譜」法和「時程分析」法進行地震作用計算,兩種方法所得結果基本一致。
  20. 70kb and 130kb in size respectively. though pks gene cluster for antibiotics fr - 008 was cloned, the genes for amino - mycosamine residue remained unknown. thus, attempt was also made to clone the desired gene ( s ), streptomyces sp

    首先從大量的發酵液中取分離了鏈黴菌fr - 008產生的抗生素fr - 008 ,並通過大孔樹脂吸附、層析等手段進行了初步
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