插入片段 的英文怎麼說

中文拼音 [chārùpiānduàn]
插入片段 英文
insertion element
  • 插 : 動詞1. （把細長或薄的東西放進、擠入、刺進或穿入; 插上; 插進） stick in; insert 2. （中間加進去; 加進中間去） interpose; insert
  • 入 : Ⅰ動詞1 （進來或進去） enter 2 （參加） join; be admitted into; become a member of 3 （合乎） conf...
  • 片 : 片構詞成分。
  • 段 : Ⅰ量詞（部分） section; segment; part; paragraph; passage Ⅱ名詞（姓氏） a surname
  • 插入 : insert; infix; run in; break in; patch; insertion; plug in; intercalate; intercalation; intromiss...
  • 片段 : part; passage; fragment; extract; segment; bit; episode; snatch; section
  1. There are three short insertions in the maize 23s gene.

    玉米的23s基因中有三個短的插入片段。
  2. Cut off beta fragment from plasmid prok. ii with hindlll and ecor i as insert, and cut pa into linear plasmid as vector fragment. link the insert and vector fragment together with t4 ligase, and the new vector with gene beta and gus was constructed

    用hind和ecor雙酶切prok質粒，獲得beta基因片段作為插入片段，用hind和ecor雙酶切a質粒作為載體片段，將插入片段與載體片段相連，即構建成含有beta和gus的雙基因載體。
  3. The individuals of rhd - positive phenotype with intact exons carried generally insert fragments and boxl box2 and box3 and this proved that inserts or rh box could n ' t affect the express of rh d gene. in 2 of the 5 wei nationality pedigrees whose proband were rh d - negative, rhc / e phenotype of all the rh negative individuals was ccee. rhd exon 4nsert and rh box did not be found in all individuals

    在7個先證者為rhd陰性的漢族家系中，大部分成員均出現插入片段和rhbox ，且在遺傳上符合孟德爾遺傳定律， d外顯子完整且表型為rhd陽性的家系個體成員廣泛帶有插入片段和box1 、 box2或box3 ，插入片段或rhbox並未影響d基因的表達。
  4. To study on structure and inheritance of rh d gene interaction between gene expression of rh d and rh c / e and influences on rh d gene expression of inserts and rh box - methods : 20 pairs of oligonucleotide specific primers for exon, intron 2 4, insert and rh box of rh d, rhc / e gene were designed and composed the polymerase chain reaction - sequence specific oligonucleotide primer ( pcr - ssp ) was used to amplify the rh c / e gene, rh d gene, exon, intron, insert and rh box in 106 samples of unrelated individuals and 7 han nationality ancestries and 5 wei nationality ancestries whose proband were rh d - negative

    目的：觀察中國漢族非血緣關系隨機個體、漢族及維吾爾族家系rh血型的c e基因、 d基因外顯子、內含子、插入片段以及rhbox ，研究rhd基因結構及遺傳規律， d基因表達與c e基因的關聯，以及插入片段和rhbox對d基因表達的影響。
  5. In this study, we at first aimed at obtaining the gene encoding the specific ige antibody related proteins by immunoscreening the schistosoma juponi cum adult worm cdna library with the pooled high - titer ige antisera from the individuals living in the schistosome epidemic regions

    J測定。 jnij序結果顯示，該插入片段為1200hp ，第一開讀框長507hp ，編碼169個氨基酸殘基，理論分子量為19 3kdi 。
  6. The minimum region of dna fragment required for an expression of red fluorescent light could be reduced to 1. 5 kb

    其中有一個亞克隆（稱為red - sac - sal ）盡管僅含1 . 5kb左右原插入片段，仍然能在紫外線下發射紅色熒光。
  7. A gene library of psedomonas fluorescens g2 was constructed in the cosmid vector pla2917 using e. coli jm109 as the host strain. two recombinants, pgr3 and pgr7, which can confer glyphosate resistance ofe. coli jm109 were identified from the selective medium containing 10mm glyphosate

    以粘粒pla2917為載體、大腸桿菌jm109為受體菌構建熒光假單胞菌g2的基因組文庫，在含有10mm草甘膦的固體選擇培養基上篩選出兩個耐受克隆pgr3和pgr7 ，插入片段分別為7kb和11kb 。
  8. Chapter 2 screening of genes related to the ovary development of the mitten crab ( eriocheir japonica sinensis ) by cdna macroarray analysis plasmids were extracted from all the clones in the subtracted cdna libraries with alkali lysis method, then the inserted fragments in plasmids were amplified by pcr

    2用cdna微陣列篩選中華絨螯蟹卵巢發育相關基因將差減cdna文庫中的所有克隆用堿裂解法提取質粒，然後以提取的質粒為模板，用pcr方法擴增插入片段。
  9. This article dealt with cloning and sequencing of chitinase and endoglucanase genes of bacillus spp. and recombinant biocontrol isolates of bacillus spp

    質粒分析證明克隆子中含有重組質粒，外源插入片段大小為2 . 6kb左右，與pcr最初產物的大小一致。
  10. This method has several strong points : ( 1 ) eliminating the possibility of ringing self of vector. ( 2 ) the inserting fragments ca n ' t ligate each other. ( 3 ) the translating rate with the partially filled in method is equal to phosphatase method

    末端半補齊技術的優點有： （ 1 ）徹底消除載體自環化的可能性； （ 2 ）消除了插入片段自身相互連接的可能性； （ 3 ）同常用的堿性磷酸酯酶法相比較，採用半補齊技術其連接產物的轉化率不受影響。
  11. Differential hybridization of human testis cdna micorarrays human testis cdna microarrays were constructed by spotted the pcr product amplified from human testis library. then membranes were hybridized using the probes of embryonic testis ( 6 months ) and adult testis

    人睪丸cdna微陣列雜交從人睪丸文庫中pcr擴增插入片段，將pcr產物點膜制備成cdna微陣列，然後提取胚胎和成人睪丸的mrna ，制備成探針，分別與微陣列進行雜交，獲取差異表達克隆。
  12. Then, the plasmid was transformed into jm109. the full length pstvd cdna recombinant plasmid was further identified by restriction mapping analysis and the nucleotide sequence of cdna cloned in pmd 18 - t vector was analyzed with ab1 377 dna sequence. test showed that the cdna of this chinese pstvd isolate was the same as pstvd - kf - 6 mild " type " isolate which originated from naturally infected potatoes in field of cornell university potato breeding program

    利用rt - pgr技術，設計一對引物，對田間採集的經鑒定含pstvd的陽性樣品進行全序列擴增，並將所得產物純化回收，連接到pmd18t - vector中，並轉化至大腸桿菌jm109中，挑選白斑進行雙酶切（ saci / ecorv ）鑒定證明插入片段為359bp大小，進行序列測定，所得克隆基因為pstvd全序列負鏈，大小為359bp 。
  13. One of them was identified as the beth gene of halobacillus sp. d8. a hydrophobicity plot of beth revealed an alteration of hydrophobic and hydrophilic segments that was characteristic for integral membrane protein, suggesting the presence of 12 transmembrane - spanning segments and belonged to bcct family

    將重組質粒測序，插入片段大小為4kb左右，通過blast比較，結果顯示含有三個orf框，其中一個為甘氨酸甜菜堿轉運蛋白，對其氨基酸組成進行疏水性分析，推測為含有12個跨膜域的跨膜蛋白，屬于bcct家族。
  14. Compared with a reported cmv - cp gene sequence, the homology of nucleotide sequences were 100 %. the sequencing result also demonstrated the recombinant vector pet - 22b - cp has a proper orf encoding 218 amino acids. the recombinant vector was transformed into bl21 ( de3 ) cells. transformants were grown and induced by the addition of isothiopropylgalactoside ( iptg ) to a concentration of imm with continued shaking at 37 ?

    酶切鑒定及序列測定表明，重組表達質粒pet - 22b - cp連接區域符合設計要求，具有正確的開放閱讀框架，插入片段含有218個氨基酸的完整編碼區，其核苷酸序列與報道的cmv - cp基因的同源性為100 。
  15. The library size is 1. 2x 106 recombinants. the average length of inserted fragments in the library was longer than 450bp, which was testified by both pcr and sequencing analysis

    藍白斑檢驗結果表明cdna文庫滴度為1 . 2 10 ~ 6 ，重組效率為95 . 9 ， pcr檢測及序列分析結果驗證插入片段平均長度大於450bp 。
  16. The predicted protein contained 441 amino acids. the activity of the epsp synthase encoded by these two subclones was shown by complementation of the aroa mutation in e. coli strain er2799, indicating two subclones possess a intact activity of the enzyme 5 - enolpyuvyl - 3 - phosphoshikimic acid synthase

    採用互補試驗驗證分離的與草甘膦耐受相關的dna片段的生物學功能，結果顯示r3h1和r7h1均能使大腸桿菌epsp缺陷型菌株er2799恢復在限制性培養基上的生長能力，證明這兩個亞克隆的插入片段具有完整的epsp合成酶功能。
  17. We sequence the inserted gene fragment of the indentified recombinant clone. the result is : angiostatin gene orf ( open reading frame ) links with the orf in expression vector correctly. but the first base of the codon aaa coding for lys414 in plg kringle 4 domain mutates from a to g which leads lys change to glu

    隨后取通過上述鑒定的重組克隆菌，對重組子插入片段測序，結果為： as基因開放讀碼框與表達載體的讀碼框正確匹配相連，但在其kringle4區相當于編碼plg的lys ~ （ 414 ）密碼子aaa的第一位堿基由a突變為g ，導致相應的氨基酸殘基突變為glu 。
  18. The library consisted of 1. 3 x 106 clones with an average insert size of about 18kb. the capacity of this library was about 20 times the equivalent of the genome of atriplex centralasiatica iljin. screening the genomic library with a 400bp probe located at the 5 ' end of the badh gene obtained by rt - pcr, we got four positive clones

    3x10 『個重組噬菌體，插入片段大小約為18kb ，含插入片段的頻率為100隊以中亞濱蓉甜菜堿醛脫氫酶門adh ）基因近5 』端的約400hp片段為探針，篩選中亞濱蓉基因組文庫，得到了4個陽性克隆。
  19. The pcr product was cloned into pmd18 - t vector. the positive recombinant clone was identified by pcr and endonuclease digest

    提取重組質粒經pcr鑒定和酶切鑒定后，對插入片段進行序列測定及分析。
  20. By cheeking the transformed bacterial colonies, we had filtrated the masculine clone successfully. the sequencing result showed that the inserting fragment contained intact coding sequences of dh. pban ( pheromone biosynthesis activating neuropeptide ) and three sgnps ( suboesophageal ganglion neuropeptide ) and the leading frame of it was correct

    測序結果表明，重組載體的插入片段中含有dh 、性信息素生物合成激活肽（ pheromonebiosynthesisactivatingneuropeptide ， pban ）及三個食道下神經節肽（ suboesophagealganglionneuropeptide ， sgnp ）的完整的編碼序列，且其閱讀框架（ readingframe ）完全正確。
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