搖混培養 的英文怎麼說
中文拼音 [yáohúnpéiyǎng]
搖混培養
英文
shaking culture- 搖 : Ⅰ動詞（搖擺; 使物體來回地動） shake; wave; rock; turn Ⅱ名詞（姓氏） a surname
- 混 : 混形容詞1. （渾濁） muddy; turbid2. （糊塗; 不明事理） foolish; stupid
- 培 : 動詞1. （在根基部分堆上土） bank up with earth; earth up 2. （有目的地使成長、壯大） cultivate; foster; train
- 養 : Ⅰ動詞1 （供養） support; provide for 2 （飼養; 培植） raise; keep; grow 3 （生育） give birth to ...
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In vitro injury models of brain slice ( ogd and nmda insult ) and primary neuronal cultures ( nmda insult ) oxygen / glucose deprivation ( ogd ) - induced injury of rat hippocampal slice in vitro the rat hippocampal slices prepared were allowed to recover in the normal artificial cerebrospinal fluid ( acsf ) bubbled with gas mixture of 95 % o2 + 5 % co2 for 1 h, then they were thansfered to glucose - free nacsf which was bubbled with gas mixture of 95 % n2 + 5 % co2. after treatment with ogd, the slices were placed into 2 % ttc solution in dark and incubated at 37 * cfor 1h. the slices were weighted and a 50 : 50 mixture of ethanol / dimethyl sulfoxide was then added to extract the formazan in dark for 24 h
離體腦片損傷模型（ ogd和nmda ）及原代培養神經元nmda損傷大鼠離體海馬腦片缺氧缺糖（ ogd ）損傷大鼠離體海馬腦片制備后，在通氧混合氣的正常腦脊液（ nacsf ）中恢復60min ，然後移入通氮混合氣的無糖腦脊液（吵化sf ）中缺氧缺糖，取出腦片與2 ttc避光37 』 c溫浴60dll ，染色后根據每克濕重加入20ml抽提液（乙醇：二甲亞礬一50 ： 50 ） ，在密閉容器內避光置24h ，測量前搖勻后取200pi至96孔板，在490urn波長，酶標儀測定各孔od值。
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