搖瓶培養 的英文怎麼說

中文拼音 [yáopíngpéiyǎng]
搖瓶培養 英文
shake flask culture
  • : Ⅰ動詞(搖擺; 使物體來回地動) shake; wave; rock; turn Ⅱ名詞(姓氏) a surname
  • : 1. (瓶子) bottle; vase; jar; flask 2. (姓氏) a surname
  • : 動詞1. (在根基部分堆上土) bank up with earth; earth up 2. (有目的地使成長、壯大) cultivate; foster; train
  • : Ⅰ動詞1 (供養) support; provide for 2 (飼養; 培植) raise; keep; grow 3 (生育) give birth to ...
  1. The cultural conditions such as temperature, fermentation period and the compound of medium are studied. the result of test show that suitable factors for both bacterium to grow and active substance to produce are 28, 200rpm and 72 hours. the bacterium is gotten through centrifuge with 8000rpm for 20min. then the bacteria is diluted and colophony named s - 8 is put into and used to absorbed active substance for 4 hours

    對該菌株發酵條件的研究表明:該菌株用馬鈴薯葡萄糖液體基發酵, 28 , 200rpm中振蕩72h可獲得高活性的發酵產物,用蘇雲金芽孢桿菌hd - 1做指示菌,將發酵液稀釋40倍生測仍可形成明顯的抑菌圈。
  2. Bp neural network - based optimization for nostoc punctiforme during flask cultivation

    神經網路對念珠藻搖瓶培養條件的優化
  3. 5. exploring fermentation synthesis of quinic acid ( qa ) using bioengineering strain under shake flask conditions synthesis of qa using recombinant e. coli constructs was examined in m9 accumulation medium. the result indicated that qa was produced from d - glucose in the culture supernatant, but it is necessary to explore further the consequences of fermentation in next work

    工程菌發酵產酸初步觀察初步探索了奎尼酸工程菌在m9積累基中的發酵產酸條件,結果表明工程菌經直接發酵由葡萄糖產生了奎尼酸,但尚須進一步優化發酵條件提高產酸率。
  4. It is the optimal time for subjecting creatine to the medium when cultured to 12h and the concentration of creatine was 0. 75 %. creatine, sarcosine and choline chloride could induce the creatinase production and creatine was the optimal inducer, but creatinine and urea could not induce the creatinase production. 3 purification of creatinase the process of creatinase purification was performed as follows : first the enzyme was completely precipitated in the range of 40 - 80 % of saturation with ammonia sulfate fraction precipitation

    最佳氮源為玉米漿和蛋白腖,最佳比例為2 : 3 ,最佳濃度為1 . 6 ;加入其它碳源時有助於菌株穩定產酶; 100ml的最佳裝液量為15ml ;肌酸、肌氨酸和氯化膽堿都能誘導菌株產酶,其中肌酸誘導產酶的效果最好,而肌酐和尿素不能誘導菌株產酶;誘導物肌酸的最適加入時間為接種12小時后,最適加入量為0 . 75 。
  5. Through screening a lot of mutants with the method of transparent zones and culture filtrate, the best four were obtained with high - yield of stable phb depolymerase, named as 02, 04, 09 and 14

    以青黴( penicillium . sp ) ds9701為出發菌株,通過紫外線誘變分生孢子,採用透明圈初篩和搖瓶培養復篩的方法,獲得4個能穩定遺傳的phb解聚酶高產菌株。
  6. An investigation has been carried out of fed batch culture according to the optimum condition of batch culture

    搖瓶培養最優條件為基礎,進行了補料實驗。
  7. Specific pichia clony pcr product showed that foreign phytase gene was integrated into the host cell. the experimental results from flask fermentation and phytase activity assay indicated that phytase gene was effectively expressed by the recombinant pichia

    挑選轉化子經過bmgy搖瓶培養、 bmmy誘導發酵后,用釩鋁酸按法測定了表達產物的酶活性,結果表明重組菌株可有效表達具有生物學活性的植酸酶。
  8. The optimal condition of fermenation was 30 " c, 48hours, and the vibration frequency was 115 rpm. 20g / l of lys concentrtion was obtained under this condition

    在500ml的三角裝液量為30ml于往復式床上振蕩,頻率為115rpm ,振幅78mm ,溫度30 ,時間48 。
  9. Flask cultivation time was 30h or so

    搖瓶培養周期以30h左右為宜。
  10. Different cultivation condition such as induced time, methanol concentration and ph of culture medium was studied at shake flask cultivation

    通過搖瓶培養初步摸索了誘導時間、甲醇濃度、基ph值等條件對mut ~ +重組菌株表達pap的影響。
  11. The ade + transformants were selected and fermented in flasks with 20ml bmmy medium, then, induced by 0. 5 % methanol. the expression protein was analyzed by sds - page after five days of induction. sds - page analysis revealed that the high - level expression recombinant strains of pmad16 / pmet a b / abp2304 and pmad16 / pmet a a / abp780 had specific bands at 75kd and 55kd separately, account for 30 % and 10 % of the total protein separately, which were purified using probond resin purification system, and obtained 15mg at levels above 0. 75g / l and 7mg expression protein at levels above 0. 35g / l separately once purification, the purity is both above 90 %

    篩選ade +表型轉化子, 20mlbmmy搖瓶培養,用0 . 5甲醇誘導表達5天後, sds - page檢測結果表明:選出的重組高效表達菌株pmad16 pmet b abp2304和pmad16 pmet a abp780都存在明顯的表達特異條帶,分子量分別為75kd和55kd ,分別占其總蛋白的30和10 ,經過probondresin鎳親和層析柱都得到了純化,其純度都在90以上,一次純化分別可得到大約15mg和7mg表達蛋白,推知表達量分別高達0 . 75g l和0 . 35g l以上。
  12. Shake - flask study on high - density culture condition of genetically engineered pichia pastoris

    高密度條件的研究
  13. The 102 strains which can produce hydrolyzed circles were obtained using alternative medium containing phytate - calcium. after being isolated and purified, these strains were inoculated into fermented medium, shaking in 28 c at 220r / min for 5 days, then their enzymatic activities were determined by ammonium molybdate - phosphate colorimertry under the condition of 37 cand ph2. 5. the result showed there were 24 strains with higher enzymatic activities among the 102 strains, after the rescreening, 7 strains were gained with enzymatic activities beyond 15u / ml and stable ability of producing acidic phytase, of which, enzymatic activity of the strain 14 was the highest, reaching 53. 86u / ml, and it was preliminarilly identified as aspergillus. niger, then numbered as aspergillus. niger 14

    用植酸鈣選擇性平板基從土樣中篩選出了102株能產透明圈的菌株,經分離純化后,接入液體發酵基, 28 、 220r min發酵5天,在37 、 ph2 . 5條件下用釩鉬酸銨法測定其所產植酸酶的活力,結果顯示,酶活較高的有24株,經再次復篩后,酶活大於15u ml且產酶性能穩定的共有7株,其中以14 ~ #菌株的酶活最高,可達53 . 86u ml ,經初步鑒定為黑麴黴,編號為aspergillus . niger14 ~ # 。
  14. In total 102 strains of acidic phytase producing strains were selected from soil by selective plate containing calcium phytate. among them 32 strains with relatively large clear circle were purified and re - selected by shaking - culturing. after fermented for 5days at 28 c and shaking at 220r / min, the activity of phytase was determined by nh4vo4 - ( nh4 ) 6mo7o24 method at 37 c and ph2. 5 or ph5. 5

    主要結果如下: 1植酸酶高產菌株的篩選利用植酸鈣選擇性子板基從土樣中篩選出102株酸性植酸酶產生菌,從中挑選出透明圈較大的菌株32株,經分離純化後分別進行復篩, 28 、 220r / min發酵5天後,在37 、 ph2 . 5或ph5 . 5條件下用釩鉬酸銨法檢測其酶活,結果發現有3株菌產酶活性較高且產酶性能較為穩定。
  15. Western blot analysis showed that rhpk - 5 ( recombinant human plasminogen kringle 5, rhpk - 5 ) protein was recognized by mab same as native hpk - 5. the result suggested that we obtained correct gene sequence of hpk - 5 and got the purified rhpk - 5. section ii : construction of pbv220 / hpk - 5 vector for obtaining high - level hpk - 5 expression system, the hpk - 5 gene was recombined with plasmid pbv220 to construct the vector of pbv / hpk - 5

    Coli )作為宿主,經sds - page分析,篩選表達量最高的菌株作為發酵用工程菌株;用western - blot方法鑒定hpk - 5因子的免疫學活性;用發酵的方法,研究發酵基的體積(溶解氧) 、組成成份及誘導起始時間和誘導持續時間對目的蛋白表達量的影響,優化hpk - 5基因工程菌的表達條件。
  16. The yeast strain 12y - 5 was identified at the species level using two yeast taxonomy systems published by. j. w. kreger - van rij ( 1984 ) [ 24 ] and j. a. barnett ( 1983 ). [ 19 ] in addition, the ability to assimilate several compounds as main sources of carbon and nitrogen was evaluated at the 500ml shake - flask and 3. 7l kfl bioreactor scales. effects of ph, temperature, concentration of dissolved oxygen and utilizable sugar on the growth rate of cells and the rate of the conversion of substrate into biomass were studied

    進而用正交試驗法對該酵母生長的基配方及影響生長的重要理化因素進行了較為深入的研究,在此基礎上對影響生長的重要理化因素進行了寬范圍的測驗:並以最佳的條件為基礎,在3 . 7升kfl -生物反應器上進行了放大試驗。
  17. The vhb expression in the strain hv was investigated by co - binding assay. in flask culture, the strain hv had almost cell densities compared with e. colihms174 in the early phase of the growth

    ,發現在前期, hv的生長與無vhb基因的對照菌e . colihms174相差不多,到後期時, vhb基因整合菌hv才略有生長優勢。
  18. The fermentation with 10l mechanically stirred fermentor was 20 hours shorter than that in the conical flasks with the highest esterase activity 14. 6 u / ml, which was 30 % than that in the conical flasks. the optimal reaction conditions of esterase were temperature 35 and ph 9. 0. the esterase was stable under 40 between 5. 0 - 9. 0

    10l發酵罐發酵與三角發酵相比,周期縮短20h ,最大酶活達到14 . 6u ml比酶活提高30酯酶的最適反應條件;溫度為35 , ph值為9 ,該酯酶在ph5 9及40以下的條件下比較穩定。
  19. Without documents related to original strain, this thesis had to probe into the primary technological conditions of original strain. and eventually made the certain radial fermentation condition

    在未提供出發菌原始資料的情況下,對出發菌的斜面基及配方、生長習性等進行了摸索,確立了基本的發酵條件。
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