搖瓶 的英文怎麼說

中文拼音 [yáopíng]
搖瓶 英文
shake flask
  • 搖 : Ⅰ動詞（搖擺; 使物體來回地動） shake; wave; rock; turn Ⅱ名詞（姓氏） a surname
  • 瓶 : 1. （瓶子） bottle; vase; jar; flask 2. （姓氏） a surname
  1. Shake - flask culture condition of lysine fermentation were studied with czl. appropriate feeding of organic niteogen sources was favorable for fermentation jn our experiment, the concentations of the yeast extract and beef extract were 15g / l and 25g / l. the initial ( nh02so4 and glucose concentrations suitable for fermentor production ranged form 55g / l and 150 - 180g / l respectively

    通過搖瓶發酵實驗初步確定czl的發酵條件為：初糖： 150 - 180g / l ，採用無機氮源和有機氮源配和使用，最佳組合是：硫酸銨質量濃度為55g / l ；酵母膏質量濃度為15g / l ，牛肉膏質量濃度為25g / l為最佳。
  2. The cultural conditions such as temperature, fermentation period and the compound of medium are studied. the result of test show that suitable factors for both bacterium to grow and active substance to produce are 28, 200rpm and 72 hours. the bacterium is gotten through centrifuge with 8000rpm for 20min. then the bacteria is diluted and colophony named s - 8 is put into and used to absorbed active substance for 4 hours

    對該菌株發酵條件的研究表明：該菌株用馬鈴薯葡萄糖液體培養基發酵培養， 28 ， 200rpm搖瓶中振蕩培養72h可獲得高活性的發酵產物，用蘇雲金芽孢桿菌hd - 1做指示菌，將發酵液稀釋40倍生測仍可形成明顯的抑菌圈。
  3. Fermentation condition of the long chain dicarboxylic acid in shake flask by candida tropicalis

    搖瓶發酵制取長鏈二元酸工藝條件的研究
  4. Shaking flask experiments and hplc analyses showed that the four mutants no longer produced the toxic oligomycin, and only made four components of avermectins b, which were avermectin b1a, b1b, b2a, b2b. the yields of avermectins b in these mutants were separately equal to those in cz8 - 73. this revealed that olma genes deletion did n ' t affect the biosynthesis of avermectins

    將4株經southern雜交驗證正確的基因缺失突變株進行搖瓶發酵和hplc檢測，發現4個突變株均不再產生寡黴素而僅產阿維菌素b組分，阿維菌素的總產量和b1的產量與出發菌株相當，說明寡黴素pks基因簇的缺失並不影響阿維菌素的生物合成。
  5. Bp neural network - based optimization for nostoc punctiforme during flask cultivation

    神經網路對念珠藻搖瓶培養條件的優化
  6. Study on the conditions for shaking flask fermentation of the exopolysaccharide production from an oligotrophic bacteria

    一株寡營養細菌胞外多糖的搖瓶發酵研究
  7. 5. exploring fermentation synthesis of quinic acid ( qa ) using bioengineering strain under shake flask conditions synthesis of qa using recombinant e. coli constructs was examined in m9 accumulation medium. the result indicated that qa was produced from d - glucose in the culture supernatant, but it is necessary to explore further the consequences of fermentation in next work

    工程菌搖瓶發酵產酸初步觀察初步探索了奎尼酸工程菌在m9積累培養基中的發酵產酸條件，結果表明工程菌經直接發酵由葡萄糖產生了奎尼酸，但尚須進一步優化發酵條件提高產酸率。
  8. It is the optimal time for subjecting creatine to the medium when cultured to 12h and the concentration of creatine was 0. 75 %. creatine, sarcosine and choline chloride could induce the creatinase production and creatine was the optimal inducer, but creatinine and urea could not induce the creatinase production. 3 purification of creatinase the process of creatinase purification was performed as follows : first the enzyme was completely precipitated in the range of 40 - 80 % of saturation with ammonia sulfate fraction precipitation

    最佳氮源為玉米漿和蛋白腖，最佳比例為2 ： 3 ，最佳濃度為1 . 6 ；加入其它碳源時有助於菌株穩定產酶； 100ml搖瓶的最佳裝液量為15ml ；肌酸、肌氨酸和氯化膽堿都能誘導菌株產酶，其中肌酸誘導產酶的效果最好，而肌酐和尿素不能誘導菌株產酶；誘導物肌酸的最適加入時間為接種培養12小時后，最適加入量為0 . 75 。
  9. Shaking flask experiments and hplc analyses showed that 99 isolates still produced four components of avermectins b, in which the yields of avermectins b in 82 isolates were only about 0. 1 ~ 2 % of those produced by the parental strain olm73 - 12. 2 of 82 isolates were confirmed to be the correct gene replacement mutants by pcr and sequence analysis. the mutant only producing avermectins bl was not detected

    搖瓶發酵和hplc分析結果表明，有99個突變株仍然產阿維菌素b的4個組分，其中82株的發酵單位很低，僅為出發菌株olm73 - 12的0 . 1 2 ，從中挑取2株經pcr擴增和測序驗證，均發生了正確的基因取代；沒有檢測到僅產b1的突變株，這表明阿維菌素b2組分的產生並不是因為阿維菌素pks上dh2的部分活性所造成。
  10. The shake flask experiments showed that b. caryophylli ys13 could accumulate polyhydroxyalkanoates containing monomers ranging from 3 - hydroxybutyrate ( 3hb ) to 3 - hydroxydecanoate ( 3hd ) when cultivated on glucose and / or fatty acids. previous study of burkholderia strain showed that this strain possessed at least two pha biosynthesis pathways, one of which is similar with ralstonia eutropha ( type i pha biosynthesis pathway )

    搖瓶發酵研究表明， biysi3可以利用葡萄糖和多種脂肪酸作為碳源，合成從4個碳原子數的聚羥基丁酸（ phb ）到10個碳原子數的聚羥基癸酸（ phd ）在內的多種pha 。
  11. Through screening a lot of mutants with the method of transparent zones and culture filtrate, the best four were obtained with high - yield of stable phb depolymerase, named as 02, 04, 09 and 14

    以青黴（ penicillium . sp ） ds9701為出發菌株，通過紫外線誘變分生孢子，採用透明圈初篩和搖瓶培養復篩的方法，獲得4個能穩定遺傳的phb解聚酶高產菌株。
  12. An investigation has been carried out of fed batch culture according to the optimum condition of batch culture

    以搖瓶培養最優條件為基礎，進行了搖瓶補料實驗。
  13. Standard test method for determining the aerobic aquatic biodegradation of lubricants or their components using the gledhill shake flask

    用gledhill搖瓶測定潤滑劑或其化合物需氧水中生物降解性的標準試驗方法
  14. Specific pichia clony pcr product showed that foreign phytase gene was integrated into the host cell. the experimental results from flask fermentation and phytase activity assay indicated that phytase gene was effectively expressed by the recombinant pichia

    挑選轉化子經過bmgy搖瓶培養、 bmmy誘導發酵后，用釩鋁酸按法測定了表達產物的酶活性，結果表明重組菌株可有效表達具有生物學活性的植酸酶。
  15. When both genes were co - expressed in e. coli, the activity of ppsa varied from 2. 1 - 9. 1 fold comparing to control, but the activity of tkta was relatively stable ( 3. 9 - 4. 5 fold ). whatever the two genes were expressed respectively or cooperatively, both could promote the production of dahp, the first intermediate of the common aromatic pathway, but co - expression was more effective on forming dahp and screened ppt - and ptp - as more effective. the results demonstrate that co - expression of ppsa and tkta can improve the production of dahp, and what ' s more, when multigenes co - expressed, the recombinant which has coordinated enzymes activity is optimum

    莽草酸途徑的最優化和整體調控基因csra的敲除正是上述改變的分子基礎，同時也為三種芳香族氨基酸的基因工程菌的構建打下了基礎; 7 .在國內外首次實現了共同途徑限制性底物關鍵酶ppsa刁無『及arog與分支途徑關鍵酶基因phea的串聯高效表達，所構建的重組質粒ptga ，其ppsa 、 tkta 、 arog 、 cm和pd的酶活分別比對照提高了3 、 2 、 2 ， 5 、 4 、 2 . 3倍，且其酶活比較協調一致; 8 .將ptga導入到篩選的基因敲除和基因替換菌株大腸桿菌31884 c甲b中，搖瓶發酵證實比以往所構建的基因工程菌株具有較高的phe產量和糖轉化率率，分別為0 . 448 %和22 . 4 % 。
  16. The transfermants with highest resistance to g418 ( 4 g / l in ypd ) were screened to study their expression level in 150 ml shaking flask. having been induced with methanol for 36 hours, the target enzyme could be examined in the supernatant by measuring amidolytic activity

    首次將大連蛇島賅蛇毒類凝血酶成熟基回克隆到表達載體ppicgk中，經電激轉化至畢氏酵母菌株gs15中，再經甲醇誘導，在150ml搖瓶畔1獲得細胞外分泌表達產物。
  17. Flask cultivation time was 30h or so

    搖瓶培養周期以30h左右為宜。
  18. Different cultivation condition such as induced time, methanol concentration and ph of culture medium was studied at shake flask cultivation

    通過搖瓶培養初步摸索了誘導時間、甲醇濃度、培養基ph值等培養條件對mut ~ +重組菌株表達pap的影響。
  19. The ade + transformants were selected and fermented in flasks with 20ml bmmy medium, then, induced by 0. 5 % methanol. the expression protein was analyzed by sds - page after five days of induction. sds - page analysis revealed that the high - level expression recombinant strains of pmad16 / pmet a b / abp2304 and pmad16 / pmet a a / abp780 had specific bands at 75kd and 55kd separately, account for 30 % and 10 % of the total protein separately, which were purified using probond resin purification system, and obtained 15mg at levels above 0. 75g / l and 7mg expression protein at levels above 0. 35g / l separately once purification, the purity is both above 90 %

    篩選ade +表型轉化子， 20mlbmmy搖瓶培養，用0 . 5甲醇誘導表達5天後， sds - page檢測結果表明：選出的重組高效表達菌株pmad16 pmet b abp2304和pmad16 pmet a abp780都存在明顯的表達特異條帶，分子量分別為75kd和55kd ，分別占其總蛋白的30和10 ，經過probondresin鎳親和層析柱都得到了純化，其純度都在90以上，一次純化分別可得到大約15mg和7mg表達蛋白，推知表達量分別高達0 . 75g l和0 . 35g l以上。
  20. Water quality - evaluation of the aerobic biodegradability of organic compounds at low concentrations - shake - flask batch test with surface water or surface water sediment suspensions

    水質.評價低濃度有機化合物有氧生物降解能力.地表水或地表水懸浮質泥沙搖瓶試驗
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